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Citations for Roche :
1751 - 1800 of 7579 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... digested for 1 hour with collagenase (Roche, 11088831001, 1 mg/ml) in RPMI medium at 37°C ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1 mM DTT and 1 × EDTA-free protease inhibitor cocktail (Roche). Lysates were clarified by centrifugation at 17,000 ×g ...
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bioRxiv - Plant Biology 2021Quote: ... 10% glycerol) containing 1 mM PMSF and 1× protease inhibitor (ROCHE) followed by incubation for 30 min at 4°C (shaking) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mL human insulin (final concentration 20 μg mL-1, Roche), 1ml BSA (final concentration 50 μg mL-1 ...
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bioRxiv - Plant Biology 2023Quote: ... 1% Triton X-100,10% glycerol) containing 1× Protease Inhibitor Cocktail (Roche) and incubated for 30 min at 4℃ ...
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bioRxiv - Neuroscience 2022Quote: ... 1% sarkosyl and protease inhibitor (1 tablet per 10 ml, Roche), and incubated for 60 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... lysed by 1 mg mL-1 Collagenase-D (Roche, Basel, Switzerland) for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mM EDTA) supplemented with 1× Complete protease inhibitor cocktail (Roche) and 2 mg/ml of fatty acid-free bovine serum albumin (BSA) ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mM EDTA) supplemented with 1× Complete protease inhibitor cocktail (Roche) and 0,2% of fatty acid-free bovine serum albumin (BSA ...
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bioRxiv - Immunology 2024Quote: ... 1 g/l glucose and 1× protease inhibitor cocktail (Roche, 5892970001). Cells were treated in microcentrifuge tubes with compound or DMSO and incubated at 37°C for 1 h.
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bioRxiv - Plant Biology 2024Quote: ... 1 mM PMSF and 1 x complete protease inhibitor cocktail (Roche). Immunoprecipitation experiments with GFP-trap beads were performed according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... the cDNA of our samples were diluted 10 fold and 4 µl were added to a mix of 5 µl FastStart Universal SYBR Green Master (Roche, Germany), 0.4 µl of nuclease-free water and 0.3 µl of forward and reverse primer (see primer sequences in Supplementary Table 1) ...
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bioRxiv - Cancer Biology 2020Quote: ... Two milligrams recombinant MYC and 12 μl T7-HCF-1VIC were rotated overnight at 4°C in Kischkel buffer + PIC (Roche 05056489001). Anti-FLAG M2 Affinity Gel (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... following a 30 min incubation at 4 °C in the presence of protease inhibitors (cOmplete™ EDTA-free Protease Inhibitor Cocktail, Roche). Following centrifugation at 16,000 g for 10 min ...
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bioRxiv - Genetics 2022Quote: ... 10% of cleared lysate was set aside to serve as input samples and the remainder was incubated at 4°C with anti-GFP antibodies (Roche #11814460001) for 3 h under gentle rotation ...
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bioRxiv - Neuroscience 2020Quote: WB was performed on cell lysates obtained by harvesting cells with lysis buffer (Tris 125 mM pH 6.8, 4% sodium dodecyl sulfate, 20% glycerol) with Complete Protease Inhibitor Cocktail (Roche, Basel, Switzerland) and sonicating ...
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bioRxiv - Molecular Biology 2023Quote: ... and CBD patients were gently homogenized at 4 °C in 5 mL of TBS buffer containing one tablet of cOmplete™ mini EDTA-free protease inhibitor cocktail (Roche) at a concentration of 20% w/vol using a Dounce homogenizer ...
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bioRxiv - Neuroscience 2023Quote: ... Each brain with known weight was homogenized at 4°C using automated homogenizer and with IX RIPA buffer containing protease inhibitor tablet (Roche, Germany). After that the brain lysates were centrifuged at 12,000 rpm for 10 mins ...
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bioRxiv - Neuroscience 2023Quote: ... The hybridization signal was revealed with NBT (nitroblue tetrazolium) and BCIP (5-bromo-4-chloro-3-indolyl phosphate) (both from Roche Diagnostics) mixed in B2 in a proportion of 0.45 µl of NBT and 4.5 µl of BCIP in 5 ml of B2 ...
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bioRxiv - Molecular Biology 2022Quote: ... and developed in a solution of nitroblue tetrazolium chloride (NBT) and 5-bromo-4-chloro-3-indoxyl phosphate (BCIP; Roche, Switzerland) for 15 minutes at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... and then the detection reaction was carried out in a buffer containing 3.5 µl/ml of of nitro blue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) (Roche, Bâle, Switzerland). The reaction was stopped with 50 mM EDTA in PBS and embryos were washed in PBSTw ...
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bioRxiv - Immunology 2022Quote: Purified DNA from mouse fecal pellets or sorted cells was subjected to 16S variable region 4 PCR amplification using barcoded 515F and 806R primers (47) and the KAPA2G Robust HotStart ReadyMix (KAPA Biosystems), with the following cycling conditions ...
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bioRxiv - Cell Biology 2024Quote: ... assay was performed following our previous publication(4, 55) and the instruction of In Situ Cell Death Detection Kit TMR red (Roche, 12156792910). Cells were cultured on coverslips for 36 h ...
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bioRxiv - Neuroscience 2022Quote: ... Cerebellar specimen gained from 4-weeks old Prpf8Y2334N mice were processed using KAPA RNA Hyperprep Kit with RiboErase (Kapa Biosystems/Roche) due to discontinuation of the Lexogen library construction kit ...
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bioRxiv - Neuroscience 2022Quote: ... Cerebellar specimen gained from 4-weeks old Prpf8Y2334N mice were processed using KAPA RNA Hyperprep Kit with RiboErase (Kapa Biosystems/Roche) due to discontinuation of the Lexogen library construction kit ...
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bioRxiv - Microbiology 2023Quote: ... All steps were performed at 4°C and buffers were all supplemented with protease inhibitor cocktail from Roche (cOmplete tablets, EASYpack). The pellets were washed once in buffer A (50 mM Tris/HCl pH8.0 ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were finally revealed with NBT (nitroblue tetrazolium) and BCIP (5-bromo-4-chloro-3-indolyl phosphate) (both from Roche Diagnostics) mixed in B2 ...
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bioRxiv - Systems Biology 2024Quote: ... 1 ng was taken as a template for a PCR reaction (Supplementary Table 4, primers F: 42-65, R: 66-77) using KAPA HiFi HotStart ReadyMix (Roche, KK2602) for adding IIlumina indices and machine adapters ...
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bioRxiv - Physiology 2024Quote: ... Blood glucose was measured after 4–5h of food withdrawal in mice using a glucose meter (ACCU-CHECK instant, ROCHE diagnostics). At a predetermined time ...
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bioRxiv - Genomics 2024Quote: ... PCR amplification of libraries equivalent to 4 pg of DNA was performed with the KAPA HiFi Uracil+ kit (Roche, Cat No.KK2802) and primers having a Y-adaptor sequence with a phosphorothioate bond(* ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 mM KCl, 0.05 mM EDTA, 10% Glycerol, 1 mM DTT, 1 mM PMSF, 1× Complete Mini protease inhibitor, Roche) resuspended in RIPA buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were harvested and incubated in buffer A (50 mM MMT pH 8.0/300 mM KCl/10 mM MgCl2/5 % glycerol) containing 1 g L-1 lysozyme/2.5 U mL-1/SmDNAse//complete protease inhibitor cocktail (Roche) for 1 h prior to lysis using EmulsiFlex C5 (Avestin ...
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bioRxiv - Cell Biology 2022Quote: ... The nuclei pellet was lysed in 400 μl RIPA buffer 1% SDS (1X PBS, 1% IGEPAL-C630, 0.5% sodium deoxycholate, 1% sodium dodecyl sulfate, protease inhibitor cocktail (Roche)) and incubated on ice for 10 min ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 1 mM protease inhibitor cocktail (1 mM EDTA, 20 mM NaF, 0.5% NP-40, 1% Triton X-100) (Roche) and 0.5% Na3VO4 on ice ...
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bioRxiv - Microbiology 2021Quote: ... and LiCl (250 mM LiCl, 1% NP-40, 1% sodium deoxycholate, 1 mM EDTA, 20 mM Tris-HCl PH8.0, complete protease inhibitor [Roche]) washing buffers ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mM Hepes pH 7.7, 1 mM MgCl2, 1 mM EGTA, 1% Triton TX100 supplemented with protease inhibitor cocktail, Roche). 20µl of HPC4 beads were supplied with 1mM Ca2+ ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mM Hepes pH 7.7, 1 mM MgCl2, 1 mM EGTA, 1% Triton TX100 supplemented with protease inhibitor cocktail, Roche). Clarified extracts were incubated for 2 h with agarose beads previously coupled to 10 µg of non-immune rabbit IgG or 10 µg of affinity purified Arpin antibodies (according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mM Hepes pH 7.7, 1 mM MgCl2, 1 mM EGTA, 1% Triton TX100 supplemented with protease inhibitor cocktail, Roche). Extracts were incubated with anti-GFP agarose beads (GFP-trap ...
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bioRxiv - Neuroscience 2020Quote: ... γ at a 1:1:1 ratio) using retro-inverso dioleoylmelittin (riDOM) 0.2 mg/nl (synthesized at Hoffman-La Roche) and PEI 25 kD 0.67 mg/ml ...
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bioRxiv - Plant Biology 2023Quote: ... Meristem-enriched samples or whole seedlings were ground in liquid nitrogen and homogenized in cold protein extraction buffer (50 mM Tris HCl pH 7.5, 10% glycerol, 1 mM DTT, 1% IGEPAL, 1× Roche EDTA-free protease inhibitor and 1× Roche phosphatase inhibitor) ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 mM NaCl, 1 mM EDTA, 1% SDS, 0.1% Na deoxycholate, 1% Triton X-100, and 13 protease inhibitor cocktail [Roche]) was collected as nuclear proteins ...
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bioRxiv - Neuroscience 2023Quote: ... the nonspecific staining was blocked with 10% normal donkey serum in PBS with 1% BSA and 0.3% triton for 1 hour and then incubated with mouse anti-BrdU (1: 1000, Roche), rabbit anti-Ki67 (1 ...
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bioRxiv - Molecular Biology 2024Quote: ... The cell pellet was resuspended in 800 μL of lysis buffer (1× PBS, 0.05% Tween 20, 1 mM DTT, and 1× EDTA-Free Complete Protease Inhibitor Cocktail [Roche]) per 25 mL of culture medium ...
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bioRxiv - Molecular Biology 2020Quote: Proliferative capacity of cells was analysed using 5-bromo-2’-deoxy-uridine (BrdU) labelling (Roche, Basel, Switzerland). Cells seeded on coverslips were pulsed with 10 μM BrdU for 60 min at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.05M Tris HCl pH 7.9, 5μM MgCl2, 5μM CaCl2, 2% SDS supplemented with 1x protease inhibitor cocktail, ROCHE) by sonication on ice ...
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bioRxiv - Microbiology 2020Quote: ... 10 mM imidazole and 2 mM β-mercaptoethanol) supplemented with DNaseI (AppliChem) and Complete Protease inhibitor (Roche). After one passage through a French press cell ...
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bioRxiv - Microbiology 2020Quote: ... Cobas SARS-CoV-2 PCR Test for the Cobas 6800 System (Roche Molecular Systems, Branchburg, NJ, USA) was used to detect the presence of SARS-CoV-2 [11].
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bioRxiv - Genetics 2022Quote: ... The PCR procedure was performed by adding 25μL 2 × KAPA HiFi HotStart Ready Mix (KAPA biosystems, KM2602) with 2.5ul primers of NEB Oligos kit and 2.5ul Illumina universal primers ...
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bioRxiv - Genetics 2021Quote: ... Worms were then resuspended in 0.4 ml Buffer B70 supplemented with 2 × cOmplete Proteinase inhibitor cocktail (Roche) and dripped into liquid N2 with 1 ml pipette tips to form small pearls ...
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bioRxiv - Immunology 2020Quote: ... Fresh tissue was initially cut into small pieces and digested with collagenase VI (2 mg/ml; Roche) for 30 minutes at 37°C ...