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Citations for Roche :
2001 - 2050 of 7579 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... DNA (2 µl) was added to the mixture with FastStart SYBR Green Master Mix kit (Roche, Basel, Switzerland) and specific primers SP_F 5’-GAC-CAG-TCG-AAC-GCA-CAT-TG-3’ and SP_R 5’-CGG-AGA-GGG-TTG-TTG-TGT-CT-3’ ...
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bioRxiv - Physiology 2024Quote: ... Glomus cells were dissociated using a mixture of collagenase P (2 mg/ml; Roche Applied Science, Indianapolis, IN), DNase (15 μg/ml ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µl RNA were incubated for 10 min at room temperature with 0.6 µg baker’s yeast tRNAPhe (Roche), 1 mM MgCl2 and increasing amounts of protein in a volume of 20 µl ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor tissue was mechanically dissociated into 2-4mm pieces and then digested in 2.5 mg/mL Liberase and 0.1 mg/mL DNase (Roche) for 1 hour at 37C ...
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bioRxiv - Plant Biology 2022Quote: ... 1 X protease inhibitor cocktail and 1 X phosphatase inhibitor cocktail from Roche) ...
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bioRxiv - Immunology 2021Quote: ... and 10% glycerol) containing 1 mM PMSF and 1 × protease inhibitor cocktail (Roche). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM EGTA and 1% Triton X-100 with protease/phosphatase inhibitor (Roche) mixture ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM DTT) with 1 mM PMSF and protease inhibitor cocktail tablets (Roche) by vigorous shaking in a fast prep cell breaker (Bio 101 ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 mM EDTA and supplemented with 1× Complete protease inhibitor cocktail (Roche) by using a Teflon pestle (Schuett Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM Na3VO4 and 1 mM NaF) supplemented with protease inhibitor cocktail (Roche) and incubated on ice for 30 min ...
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bioRxiv - Plant Biology 2022Quote: ... 1 mM DTT and 1× cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche). Fifty micrograms of total protein were loaded onto SDS-PAGE gels and separated by electrophoresis ...
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bioRxiv - Microbiology 2024Quote: ... Lysozyme 1 mg/ml and 1 tablet of EDTA-free protease inhibitor (ROCHE). Cells were broken passing twice through an Emulsiflex C5 (ATA scientific ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 1 mM EDTA and 1×Complete EDTA free-tablet (Roche Diagnostics, Basel, Switzerland) in distilled water ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM DTT and 1 tablet of complete EDTA-free (Roche, Basel Switzerland)] ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM DTT and 1 tablet of complete EDTA-free (Roche, Basel Switzerland)] ...
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bioRxiv - Neuroscience 2023Quote: ... in a 1:1 ratio and added to white 384 well plates (Roche). Plates were run on a 45-cycle protocol using the LC 480 II system (Roche).
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bioRxiv - Cell Biology 2024Quote: ... 1 mM Na3VO4 and 1 mM NaF) supplemented with protease inhibitor cocktail (Roche) and incubated on ice for 30-60 min ...
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bioRxiv - Developmental Biology 2022Quote: ... 25mM NaCl, 1 mM EGTA, 1.5 mM MgCl2, 1 mM DTT, 1% Triton TX-100, 10% Glycerol, 1X Roche Complete protease inhibitors) during 1h at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Tumor tissues were minced into 1×1 mm fragments and enzymatically dissociated in HBSS containing 1 mg/ml collagenase A (#11088793001; Roche, Basel, Switzerland) and 1 μg/ml DNase I (#07900 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell pellets were lysed in 1500 μl ice cold RIPA buffer (50 mM Tris-Cl pH 7.4, 150 mM NaCl, 1% NP40, 0.5% Na-deoxycholate, 0.1% SDS, 1 mM EDTA, Roche cOmplete and 1 mM PMSF). Cell lysates were clarified by centrifugation (13000 x g at 4°C for 10 min ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 mM EDTA) supplemented with 1 mM PMSF and 1 tablet of cOmplete Protease inhibitor (Roche, cat. No. 11 873 580 001) per 50 ml using a MP Biomedical Fast-Prep-24 5G bead beater ...
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bioRxiv - Neuroscience 2024Quote: ... volume of lysis buffer (25 mM HEPES pH 7.5, 200 mM NaCl, 1 μg ml−1 benzonase, 1 mM PMSF and Roche protease inhibitor tablets). Cells were lysed by dounce homogenization and the NALCN complex was subsequently solubilized by addition of 2% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... volume of lysis buffer (25 mM HEPES pH 7.5, 150 mM NaCl, 1 μg ml−1 benzonase, 1 mM PMSF and Roche protease inhibitor tablets). Cells were lysed by dounce homogenization and the NALCN complex was subsequently solubilized by addition of 2% (w/v ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting pellet was washed four times with buffer II (10 mM Tris–HCl pH 8.0, 10 mM MgCl2, 0.25 M sucrose, 1% triton X-100, 1 mM DTT, 0.1mM PMSF, 1% Roche protease inhibitor cocktail tablet) until the green color was completely removed ...
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bioRxiv - Microbiology 2020Quote: ... The cross-linking reaction was quenched by incubating the cells with 0.125 M glycine for 5 min with mild agitation at room temperature followed by centrifugation at 3,000 rpm for 5 min at 4°C with a subsequent PBS wash (containing 0.01X protease inhibitor cocktail or PIC; #11836170001, Roche Applied Science, Indianapolis, USA). Following the complete removal of PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... 5-bromo-4-chloro-3-indolylphosphate (BCIP) and nitroblue tetrazolium chloride (NBT) according to the manufacturer’s protocol (Roche Applied Science, Indianapolis, IN, USA). The sections were then mounted ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by incubation (16 h at 4 °C) in the presence of 10 pM TBST containing complete TM protease inhibitor cocktail (Roche Pharma, Basel, Switzerland). The membranes were washed three times with TBS (150 mM NaCl ...
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bioRxiv - Developmental Biology 2020Quote: ... for 16-20h incubation at room temperature and colorimetric signals were detected using Nitro blue tetrazolium chloride and 5-bromo-4-chloro-3-indolyl phosphase (NBT-BCIP; Roche Applied Sciences 11383221001). RNAScope ISH was conducted for FGF15 and Ptch1 ...
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bioRxiv - Biochemistry 2021Quote: ... The band corresponding to the crosslinked nanobody-antigen complex was then excised from the gel and subjected to in-gel digestion with trypsin (Roche, 1μg, 4 h) or chymotrypsin (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... Oocytes were then incubated for 60 min at 4 °C with 100 μg/ml rat monoclonal anti-HA antibody (clone 3F10, Roche Pharmaceuticals, Basel, Switzerland) in 1% BSA/ND96 ...
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bioRxiv - Molecular Biology 2022Quote: ... Antimouse secondary antibodies conjugated with alkaline phosphatase was used to detect color signal formed by BCIP/NBT (5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium) substrates (Roche Biochemical, Mannheim, Germany).
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bioRxiv - Cell Biology 2022Quote: ... Cells at 70% confluence were harvested by trypsinization (after 3-4 hours treatment with 100 ng/mL colcemid [Roche #10295892001] for metaphase spreads), washed with PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Strand-specific RNA libraries were prepared for sequencing (3 - 4 biological replicates/treatment) using a KAPA Stranded mRNA-Seq Kit (Kapa Biosystems, MA, USA). Poly-A mRNAs were purified from 100 ng of total RNA using poly-T-oligo-magnetic beads (Kapa Biosystems ...
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... 1% NP-40, 0.5% sodium deoxycholate, 1 mM EDTA pH 8 and 1 tablet of protease inhibitor cocktail Roche cOmplete (Roche, Basel, Switzerland) and 1 tablet of phosphatase inhibitor PhosSTOP (Roche)) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with the primary antibodies diluted in blocking solution (H3K9me3: ab8898, 1:500, HP1α: CST #2616, 1:200, HA: Roche 3F10, 1:250) for 1h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates were prepared by incubating cells in RIPA lysis buffer (25 mM Tris pH 7.6, 150 mM NaCl, 1% NP40, 1% deoxycholate, 0.1% SDS, 1X protease inhibitor cocktail from Roche and 1 mM of PMSF) on ice for 30 minutes and cell lysate was clarified by centrifugation at high speed (15 000 rpm ...
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bioRxiv - Physiology 2022Quote: ... cell pellets were resuspended in RIPA Buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% IGEPAL CA-630, 1% deoxycholate, 1 mM DTT, DNase, and Roche cOmplete Protease Inhibitor Cocktail) and lysed via sonicating water bath ...
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bioRxiv - Microbiology 2023Quote: ... 1% NP-40, 1 mM EDTA, 1 mM EGTA, 0.1% SDS, 1X EDTA-free protease inhibitor cocktail [#04693132001, Roche], and 0.5% sodium deoxycholate) and collected using a cell scraper ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.5, 150 mM NaCl, 1 mM EDTA, 10 % [v/v] glycerol, 1 mM DTT, and 1 × complete Protease Inhibitor [Roche; Cat. No. 058929700001]) was added to homogenised plant material (100 μl extraction buffer per 100 mg plant material) ...
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bioRxiv - Biochemistry 2023Quote: ... 0.2% Triton X-100, 1 mM EDTA, 1 mM dithiothreitol, 1 mM NaF, 10 mM β-glycerophosphate, 0.1 mM Na3VO4, 1x Roche cOmplete protease inhibitors). Protein lysates (12 µg per lane ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were subsequently lysed (1 mM NaF, 1 mM Na3VO4, 10 nM calyculin A, 1 mM PMSF, 1 mg complete EDTA-free protease inhibitor cocktail [Roche] in RIPA buffer), scraped ...
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bioRxiv - Biochemistry 2022Quote: ... 1% NP-40, 150 mM NaCl, 1 mM EDTA pH 8.0, 1 mM EGTA pH 8.0 and Roche complete protease inhibitor EDTA-free) and acid-washed glass beads using a Precellys machine (6 × 30 seconds ...
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bioRxiv - Cell Biology 2022Quote: ... 1% SDS, 1 mM EDTA, 1 mM DTT, 1 mM Na3VO4×2H2O, 5 µM pepstatin A, 10 µM leupeptin, 2X Roche protease inhibitor cocktail), 200µl glass beads were added ...
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bioRxiv - Genetics 2022Quote: ... 5 mM EDTA. 0.2% Triton X-100, 1 mM PMSF, 1% Glycerol, 1 pellet/50 ml EDTA free Protease inhibitor [Roche] and 25 μM MG132). 100 μl diluted mixture was used as input ...
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bioRxiv - Neuroscience 2024Quote: ... rat anti-Elav (1:200) (DSHB), mouse anti-β-galactosidase (1:200) (DSHB), Rabbit anti-Wallenda (1:100, (5)) mouse anti-GFP (1:200, Roche, Basel, Switzerland), Rabbit anti-Ref(2)P (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... 50 mM Tris/HCl pH 7.4/50 mM NaF, 10 mM Na4P2O7, 2 mM MgCl2 and Complete Mini, EDTA-free protease inhibitor [Roche]). Lysates were cleared and incubated with GFP-Trap agarose beads for 60 min ...
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bioRxiv - Cell Biology 2020Quote: ... 2% SDS and 50 mM DTT with the addition of anti-protease (cOmplete cocktail, Roche 11 873 588 001). Samples were boiled 5 min at 95°C before loading on polyacrylamide gels ...
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bioRxiv - Cell Biology 2020Quote: ... Monoclonal (3F10) antibody directed against the HA epitope and monoclonal (B-2) antibody against GFP were purchased from Roche and Santa Cruz Biotechnology (Dallas ...
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bioRxiv - Immunology 2021Quote: ... Culture media was supplemented with 500 U/mL human IL-2 (Tecin from Roche, kindly provided by the NIH) starting on culture day 2 ...
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bioRxiv - Developmental Biology 2021Quote: ... 2-μl aliquots of all cDNA samples were analyzed in triplicate on 96-well optical PCR plates (Roche Diagnostics). GAPDH or TBP was used as the reference gene and all analyses were performed using the ΔΔCt method with Roche LightCycler 96 system software ...