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Citations for Roche :
1701 - 1750 of 6574 citations for Adrenocorticotropic Hormone ACTH ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... the remaining dermis was washed in Ca2+ and Mg2+ free PBS 5 times and incubated in a digestion buffer containing 2 mg/ml collagenase A (Roche), 100 µg/ml of DNase I (Sigma ...
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bioRxiv - Genomics 2021Quote: ... Cells from two biological replicates were lysed with ChIP lysis buffer (5 mM PIPES pH8, 85 mM KCl, 1% Igepal) with a protease inhibitor (PI) cocktail (Roche). Nuclei were collected by centrifugation at 2,000 rpm ...
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bioRxiv - Genomics 2020Quote: ... 60 μl of anti-mouse magnetic beads were washed PBS/BSA (5 mg/ml BSA) and incubated with 3 μl of anti-Myc (Roche) or Anti-Rpb3 antibodies (Neoclone ...
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bioRxiv - Immunology 2021Quote: ... Lungs were cut into small pieces and digested in 5 mL of RPMI containing DNase I (100 μg/mL, Roche), Dispase II (800 μg /mL ...
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bioRxiv - Microbiology 2021Quote: ... Cells were harvested by centrifugation at 3,800 × g for 10 min at 4 °C and resuspended in lysis buffer (50 mM NaH2PO4, 300 mM NaCl, 50 mM imidazole, 5% glycerol and Complete Protease Inhibitor Cocktail Tablet (Roche), pH 8.0) ...
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bioRxiv - Physiology 2020Quote: ... RBC lysis and post-lysis wash buffers contained 5 mM Tris-HCl/pH 8.0 and complete Protease Inhibitor Cocktail tablets (cPIC; Roche, 04693116001), as opposed to sodium-phosphate buffer/pH 7.5 and PMSF and pepstatin A—a modification required to obtain good mass-spectrometry data ...
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bioRxiv - Neuroscience 2019Quote: The brain samples were homogenised in 5 volumes (w/v) of sterile PBS containing cOmplete Mini EDTA-free Protease Inhibitor Cocktail tablets (Roche), sodium fluoride (10 mM ...
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bioRxiv - Plant Biology 2021Quote: ... The abundance of specific transcript was measured by probing 1 μL cDNA by quantitative real-time PCR in a total volume of 10 µl containg 5 μL SYBR-green master mix (Roche), 0.5 μM forward and reverse primer and water ...
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bioRxiv - Microbiology 2020Quote: ... Membranes was incubated secondary antibody for an hour in 5% milk and developed using Lumi-light western blot substrate (Roche) to detect HRP ...
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bioRxiv - Microbiology 2020Quote: ... for 30 minutes at 37°C and 5 µg was converted to cDNA by reverse transcription using Transcriptor reverse transcriptase (Roche) and random hexaoligos (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5% NP-40 and supplemented with RNase inhibitor (Murine, M0314, New England Biolabls) and protease inhibitors cocktail (cOmplete Mini, Roche), and passed through a 25G needle 7 times ...
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Programmed ER fragmentation drives selective ER inheritance and degradation in budding yeast meiosisbioRxiv - Cell Biology 2021Quote: ... Pellets were resuspended by bead beating for 5 min in 100 μL TE supplemented with 3 mM and 1x protease inhibitors (Roche) with 100 μL acid-washed glass beads ...
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bioRxiv - Biophysics 2021Quote: ... The pellet was re-suspended in lysis buffer (50 mM HEPES, 500 mM NaCl, 5% glycerol, pH 7.5) supplemented with cOmplete™ protease inhibitor cocktail (Roche), 10 μg/mL DNAse I ...
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bioRxiv - Biophysics 2021Quote: ... The pellet was re-suspended in lysis buffer (50 mM HEPES, 500 mM NaCl, 5% glycerol, pH 7.5) supplemented with cOmplete™ protease inhibitor cocktail (Roche), 10 μg/mL DNAse I ...
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bioRxiv - Biophysics 2021Quote: ... The pellet was re-suspended in lysis buffer (50 mM HEPES, 500 mM NaCl, 5% glycerol, pH 7.5) supplemented with cOmplete™ protease inhibitor cocktail (Roche) and split into 6 fractions ...
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bioRxiv - Immunology 2022Quote: ... Precipitated pellet was then resuspended in 100 μL of RIPA buffer plus laemli supplemented with 5 mM diisopropylfluorophosphate (DFP) and protease inhibitor cocktail (Roche) and heat denaturated for 10 min at 95°C ...
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bioRxiv - Neuroscience 2022Quote: ... The flow through was discarded and the beads washed 5 times with wash buffer (CL48 diluted ¼ in PBS and supplemented with complete protease inhibitor cocktail (Roche)) and sample buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... then lysed for 10 min on ice with IP lysis buffer (10 mM Tris-HCl pH 7.6, 150 mM NaCl, 0.5% NP-40, 5% Glycerol and Roche protease inhibitor). Lysates were centrifuged at 17,000 × g for 10 min and supernatant incubated overnight at 4 °C with 2 μg of anti-PTBP1 antibody (monoclonal-ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: Sense and anti-sense DIG-labelled RNA probes were synthesised from 5’ and 3’ regions of tert using DIG RNA Labelling Mix (Roche). A 562bp 3’ region of tert ...
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bioRxiv - Pathology 2022Quote: ... and brain tissues were homogenized using a Teflon-glass homogenizer in ice-cold homogenization buffer (0.32 M sucrose, 5 mM EDTA, 10 mM Tris-HCl, and pH 7.4, phosphatase inhibitor cocktail tablet; Roche, Mannheim, Germany), centrifuged at 4500 rpm for 10 min at 4°C and the supernatants were collected.
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bioRxiv - Biochemistry 2022Quote: ... purified Q23-huntingtin (5 µg) was purified in the presence of complete protease and phosphatase inhibitor cocktails (Roche Applied Science) and incubated with or without PRKACA (20 ng) ...
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bioRxiv - Molecular Biology 2022Quote: ... Yeast pellets of approximately 50 OD600 units in size were resuspended in 600 µL of lysis buffer (5% SDS, 50mM TEAB, supplemented with protease and phosphatase inhibitor tablets (Roche)) and the 2 mL screwcap tube was filled to capacity with acid-washed glass beads ...
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bioRxiv - Neuroscience 2022Quote: Mounted coronal cryosections were rinsed in PBS for 3 times (5 min) and thereafter incubated in Blocking Reagent (Roche Diagnostics) for 15 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were harvested by centrifugation at 7000g at 4°C for 10 minutes and resuspended in 25 mL of cold TBS buffer (50 mM Tris-HCl, 500 mM NaCl, pH 8) with 5% glycerol and EDTA-free protease inhibitors cocktail (Roche). Cells were lysed by sonication and purified using Glutathione-Sepharose 4B (Cytiva ...
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bioRxiv - Neuroscience 2022Quote: ... homogenized on ice in 5 volumes (w/v) of T-per extraction buffer complemented with protease inhibitor tablets (Complete Mini Protease Inhibitor Tablets, Roche) and phosphatase inhibitor cocktail tablets (phosSTOP ...
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bioRxiv - Cell Biology 2022Quote: ... 30 s at 72°C) and final extension 5 min at 72°C using KAPA Taq DNA polymerase (KAPA BIOSYSTEMS). PCR products were visualized on 2% agarose gels containing SYBR Safe DNA gel stain (Invitrogen) ...
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bioRxiv - Developmental Biology 2022Quote: ... Signal was revealed with 4 μL of NitroBlue Tetrazolium/5-Bromo-4-Chloro-3-Indolyl Phosphate (NBT/BCIP) Stock Solution (Roche)/ml of AP reaction buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Glycerol was added to a final concentration of 10% as well as Turbonuclease (5 µL) and a protease inhibitor cocktail tablet (Roche). The cells were lysed by sonication (70% amplitude,10 s pulse/10 s pulse off for 2.5 min) ...
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bioRxiv - Neuroscience 2021Quote: ... This was spun down (5 minutes at 1800 RMP, 4°C) and the pellet then digested in 10ml collagenase/dispase (1mg/ml; Roche) and DNase I type IV (40µg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... un-probed input RNA was also subjected to Sanger sequencing: RNA was dissolved in 5 μl water and supplemented with 1 μl of 10 mM corresponding ddNTP (Roche), then reverse-transcribed into cDNA and analysed by PAGE as described below.
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bioRxiv - Molecular Biology 2021Quote: ... pre-let-7 and pre-miR-16 molecules (miRVana) were radioactively 5’-labeled with 32P isotopes by incubation with 32P-ƴATP and the phosphorylating enzyme T4 PNK (Roche) for 30 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... 30 mM imidazole, 1mM DTT, 0.75% CHAPS, 1 μM DNases, 5◻mM MgCl2 and EDTA-free protease inhibitor cocktail [Roche]). Purification was similar as the above instead of the elution buffer used (50 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed 5 times with ice-cold PBS and lysed in RIPA buffer supplemented with cOmplete Protease Inhibitor Cocktail (Roche). Cell lysis was facilitated by sonication and following centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBST with 5% milk protein and probed with the following antibodies: 1:1000 anti-HA (Roche), then 1:1500 goat anti-rat IgG-HRP (Dako) ...
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bioRxiv - Neuroscience 2020Quote: ... nt 588-682 from sequence NM_001110222 detected with the mDCX probe (Universal ProbeLibrary probe #68, Roche, Cat.No. 04688678001; 5′-(Fam)-CTGCTCCT-(Dark Quencher)-3′) ...
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bioRxiv - Neuroscience 2020Quote: ... nt 172–244 from sequence NM_011443 detected with the mSOX2 probe (Universal ProbeLibrary probe #63, Roche, Cat.No. 04688627001; 5′-(FAM)-AGGAGGAG-(Dark Quencher)-3′) ...
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bioRxiv - Neuroscience 2020Quote: ... nt 33-140 from sequence NM_016967 detected with the mOLIG2 probe (Universal ProbeLibrary probe #21, Roche, Cat.No. 04686942001; 5′-(Fam)-CAGAGCCA-(Dark Quencher)-3′) ...
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bioRxiv - Neuroscience 2020Quote: ... nt 376-486 from sequence NM_008742 detected with the mNT3 probe (Universal ProbeLibrary probe #49, Roche, Cat.No. 04688104001; 5′-(Fam)-GGCCACCA-(Dark Quencher)-3′) ...
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bioRxiv - Neuroscience 2020Quote: ... nt 225-323 from sequence NM_001244198 detected with the mPAX6 probe (Universal ProbeLibrary probe #67, Roche, Cat.No. 04688660001; 5′-(Fam)-CTCCAGCA-(Dark Quencher)-3′) ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Systems Biology 2022Quote: Limb buds were dissected and incubated for 5 min in 270 µL of 0.22 Wunsch unit/mL Liberase TM (Roche, 5401119001) in EDTA-free ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Embryos were then washed with wash buffer and their nuclei stained with 5 ug/mL DAPI (4′,6-diamidino-2-phenylindole, Roche) prepared in wash buffer for 10 minutes at 30 ºC.
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bioRxiv - Genomics 2022Quote: ... Pellets were harvested after 16-18h and resuspended in lysis buffer (50 mM Tris-HCl, 500 mM NaCl, 5% glycerol, 1 tablets per 50 ml protease inhibitor (EDTA-free, Roche), 0.2 mM PMSF ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were incubated with an equimolar amount of DMSO as negative control and with phytohemagglutinin (5 μg/ml) (PHA, Roche) as a positive control ...
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bioRxiv - Genetics 2022Quote: ... Expression patterns were visualized with a Nitro-Blue Tetrazolium Chloride/5-Bromo-4-Chloro-3-Indolyphosphate p-Toluidine Salt (NBT/BCIP) system (Roche). Sections were mounted with Vectamount (Vector laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were centrifuged at 300 g for 5 minutes and red blood cells were lysed from samples using RBC lysis buffer (Roche) in 5 ml for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... resuspended in 1 mL ice-cold lysis buffer (100 mM NaCl, 5% glycerol, 1 mM dithiothreitol and protease inhibitor cocktail (cOmplete, EDTA-free; Roche) in 50 mM Tris HCl ...
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bioRxiv - Neuroscience 2019Quote: ... cells were lysed in Hepes buffer (20 mM Hepes, 1% CHAPS, 100 mM NaCl, 5 mM EDTA, protease inhibitor cocktail (Roche), phosphatase inhibitor cocktail III (Sigma)) ...
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bioRxiv - Biochemistry 2019Quote: ... were lysed with lysis buffer (25mM Tris, 150mM NaCl, 1mM EDTA, 1% NP-40, 5% Glyceral) with proteinase inhibitor cocktail (Roche) on ice for 30 min ...
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bioRxiv - Immunology 2020Quote: ... Cells were cultured for 24 hours in the presence of SARS-CoV-2 specific MPs [1 μg/mL] or 5 μg/mL phytohemagglutinin (PHA, Roche) in 96-wells U-bottom plates at 1×106 PBMCs per well ...