Labshake search
Citations for Roche :
1501 - 1550 of 6235 citations for Adrenocorticotropic Hormone ACTH ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... Each 5 μl reaction contained 2.5 μl of LightCycler 480 SYBR Green I Master mix 2X (Roche Applied Science), 0.5 μl of each primer (300 nM final ...
-
bioRxiv - Microbiology 2023Quote: ... 0.5 mM EDTA, 0.1 % NP-40, 20 mM KCl, 150 mM NaCl, plus a protease inhibitor cocktail from Roche) and centrifuged for 2 minutes and 2,500 g at 4 °C ...
-
bioRxiv - Molecular Biology 2023Quote: A 20% w/v homogenate was prepared with a piece of snap-frozen liver tissue in homogenization buffer (50 mM Tris-HCl [pH 7.4], 250 mM sucrose, 25 mM KCl, 5 mM MgCl2, 3mM imidazole, supplemented with protease inhibitors [Roche]). To remove nuclei and debris ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with 500 μl acid-washed glass beads in 500 μl GFP-Trap pulldown buffer (20 mM HEPES, pH 7.4, 150 mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with 500 μl acid-washed glass beads in 500 μl GFP-Trap pulldown buffer (20 mM HEPES, pH 7.4, 150 mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...
-
bioRxiv - Developmental Biology 2023Quote: Lungs extracted from E14.5 or E18.5 embryos were minced and digested using collagenase I and dispase I (both 1 mg/mL, Roche) and incubated at 37°C for 5 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... The pellet was thawed in an equal volume of lysis buffer (1M NaCl, 25mM Tris pH7,5, 5% Glycerol,1mM DTT, protease inhibitors (Roche)) and sonicated on ice (10 pulses of 10 seconds) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The pellet was thawed in two volumes of lysis buffer (3M NaCl, 100mM Tris pH7,5, 10% Glycerol, 0.5mM EDTA, 5 mM β-mercaptoethanol, protease inhibitors (Roche)) and sonicated on ice (10 pulses of 10 seconds) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The amplification of the oligonucleotide pool was performed with ∼5 ng of template DNA and KAPA HiFi polymerase (Roche) with the annealing temperature at 53°C and extension time of 15 sec for 14-20 cycles ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and 1X Triton buffer (100mM Tris-HCl, pH8.8, 100mM NaCl, 5mM EDTA and 5% Triton-X) with protease inhibitors (11836170001, Roche) for 16 hours at 4° C ...
-
bioRxiv - Biochemistry 2023Quote: ... under gravity flow, washed with PER2 buffer (1 x TBS pH 7.5, 1mM EDTA, 5% glycerol, EDTA-free protease inhibitors (Roche)) and eluted with 3x flag peptide in PER2 buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... then the pellet was resuspended in 40 ml binding buffer (50 mM NaPi, 300 mM NaCl, 5 mM imidazole, pH 7.5) containing protease inhibitor (Roche cOmplete EDTA-free protease inhibitor cocktail ...
-
bioRxiv - Microbiology 2023Quote: ... and resuspended in lysis buffer (50 mM PBS pH 7.4, 1 mM EDTA, 5% glycerol, 1 mM PMSF and protease inhibitors from Roche). Cell lysis and protein extraction was achieved through mechanical lysis using glass beads and Precellys®24 Homogenizer (3 cycles of 30 seconds at 5500 rpm ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein was purified from these cells by freeze-thaw lysis in Hepes buffer (20mM Hepes; pH 7.5, 150mM NaCl, 5% glycerol) with Complete protease inhibitor tablets (Roche) or Phenylmethylsulfonyl fluoride (PMSF) ...
-
bioRxiv - Cell Biology 2023Quote: ... 5 % glycerol and 0.5 mM TCEP supplemented with 5 Units/mL Benzonase and an EDTA-free protease inhibitor cocktail (Roche)) ...
-
bioRxiv - Genetics 2023Quote: ... the cell pellet was resuspended in 750 μl Tris lysis buffer (50 mM Tris, 0.2% NP-40, 150 mM NaCl, 5 mM EDTA, 5.7 mM PMSF, and 1 x Roche cOmplete). For whole organ samples (either whole detunicated testis or thymus) ...
-
bioRxiv - Biochemistry 2023Quote: ... The pellet was thawed in two volumes of lysis buffer (3M NaCl, 100mM Tris pH7,5, 10% Glycerol, 0.5mM EDTA, 5 mM β-mercaptoethanol, protease inhibitors (Roche)) and sonicated on ice (10 pulses of 10 seconds) ...
-
bioRxiv - Developmental Biology 2024Quote: ... heads were rinsed for 5 minutes in PBST five times and incubated in Blocking Reagent (Roche, 1:5000 dilution) for 60 minutes ...
-
bioRxiv - Cell Biology 2024Quote: Cells or tissues were washed with PBS and lysed in an appropriate amount of protein lysis buffer (TritonX100 -1%, Tris 7.4 -50mM, EDTA -5mM, NaCl -150mM, and glycerol -5%) containing Protease Inhibitor Cocktail (Roche). We used Phosphatase Inhibitor Cocktail (Millipore ...
-
bioRxiv - Neuroscience 2024Quote: ... .5 µL of a 10 µM primer mix and 7.5 µL of Fast Start SYBR Green Master Mix (Roche). Each reaction was performed in triplicate ...
-
bioRxiv - Physiology 2024Quote: ... Sections were dried and denatured for 10 min at 80°C in hybridization buffer (0.1M Tris, pH 7.2, 25mM MgCl2, 70% formamide [Sigma-Aldrich, Saint Louis, MO], 5% blocking reagent [Roche] with 1.0 μg/mL of Cy-3-labeled telomere-specific [CCCTAA] peptide nucleic acid [PNA] probe [TelC-Cy3 ...
-
bioRxiv - Developmental Biology 2024Quote: ... 5 ng amplified DNA and 1 μM forward and reverse primers and run on a LightCycler 480 machine (Roche) using the following protocol ...
-
bioRxiv - Neuroscience 2024Quote: ... The template plasmid was diluted to 5×107 copies/µL with nuclease-free water plus yeast RNA (Roche, 10109223001), and this solution was further diluted to 2.5 ...
-
bioRxiv - Molecular Biology 2024Quote: ... 150 mM NaCl, 0.5% NP40, 0.5% Triron-X100, 5% glycerol, 1x Roche Complete EDTA free inhibitor cocktail, 1x Roche PhosSTOP) for 1h on ice by agitation ...
-
bioRxiv - Microbiology 2024Quote: ... with their respective dilution in 5% skimmed milk in PBS-tween 0.1% were used: anti-HA-HRP (3F10) (N°12013819001, Roche) 1/10,000 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
-
bioRxiv - Cell Biology 2024Quote: ... containing 5% beta-mercaptoethanol and protease and phosphatase inhibitors (Roche Complete Mini protease inhibitor, Sigma-Aldrich #11836153001, and Roche Complete Ultra phosphatase inhibitor ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
-
bioRxiv - Plant Biology 2023Quote: ... Samples were then ground to a fine powder in a mortar and pestle over liquid nitrogen and resuspended in freshly prepared extraction buffer (2mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% v/v NP40, 5% v/v glycerol, 1mM PMSF, 1ml Roche Protease Inhibitor cocktail per 30g input tissue ...
-
bioRxiv - Neuroscience 2024Quote: ... underwent lysis through the addition of 200 µl of a 50 mM Tris-HCl buffer (pH 7.8) containing 5% SDS and cOmplete ULTRA protease inhibitor (Roche). The samples were then subjected to Bioruptor® treatment (Diagenode ...
-
bioRxiv - Neuroscience 2021Quote: ... The extracted viral DNA and a serial dilution of the transfer plasmid DNA containing the WPRE sequence were transferred to a 96 well plate and measured using a LightCycler® 96 instrument (Roche Life Science). AAVs produced had a titer of 2.10 × 1013 viral genomes/ml.
-
bioRxiv - Neuroscience 2020Quote: ... reverse-transcribed into first-strand cDNA and quantitative PCR (qPCR) amplification was performed in 96-well plates in a Mastermix for probes (Roche, Burgess Hill, UK) and run on a LightCycler® 96 System (Roche) ...
-
bioRxiv - Plant Biology 2019Quote: ... were used as template for quantitative real time RT-PCRs which were performed in 384-well plates using a LightCycler 480 II detection system (Roche Diagnostics, Mannheim, Germany). GoTaq PCR Mastermix (Promega ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... CaCo-2 and Vero cells seeded at a density of 5 × 103 cells and 7 x 103 per well in 200 μl media containing 10% FBS in a 16-well E-plate (Roche Applied Science, Germany), respectively ...
-
bioRxiv - Molecular Biology 2023Quote: ... the Roche LightCycler® 480 System (Cat# 05015243001) in combination with 384-well plates (FrameStar, Cat# 4ti-0381/DBC, and Roche, Cat# 04729757001) was used ...
-
bioRxiv - Biochemistry 2022Quote: ... 1 ml of serum-free conditioned medium from parental RPE-1 or D12 cells (n=4 cultures for both) was collected after 48 hours culture in a 12-well plate and EDTA-free protease inhibitor (Roche, catalog no.11836170001) was added ...
-
bioRxiv - Developmental Biology 2023Quote: ... Real-time quantitative RT-PCR (Q-PCR) was performed in 384-well plates using the LightCycler® Multiplex DNA Master (Roche Life Sciences) and hybridization probes from the Universal Probe Library Set (Roche Life Sciences) ...
-
bioRxiv - Genomics 2020Quote: ... and the sequencing library was constructed using SMARTer v4 Ultra Low Input RNA Kit for Sequencing (Clonetech) and KAPA HyperPlus Library Preparation Kit (KAPA BIOSYSTEMS). The RNA-Seq library was multiplexed and sequenced on the NextSeq 500 platform (Illumina ...
-
bioRxiv - Microbiology 2022Quote: Total genomic DNA was extracted from the bacterial pellet as described previously [28] using the PowerFood™ Microbial DNA Isolation kit (MoBio Laboratories Inc., Carlsbad, USA) and the High Pure PCR Template Preparation kit (Roche Diagnostics Ltd ...
-
bioRxiv - Genomics 2020Quote: ... Library quantification was performed using the Qubit dsDNA High Sensitivity kit and quantitative PCR with the KAPA Quantification kit (Roche #KK4854) on a QuantStudio 5 real-time PCR system.
-
bioRxiv - Immunology 2021Quote: Total RNA was extracted from 10μm-sections of FFPE tissues using a commercial kit (High pure FFPET RNA isolation kit, Roche, Cat.6650775001) and resuspended in 25μl final volume ...
-
bioRxiv - Microbiology 2020Quote: ... or the 5’-flanking region of Bcflp2 (amplified with Probe2For and Probe2Rev primers) using the PCR DIG Probe Synthesis Kit and the DIG Luminescent Detection Kit (Roche, Germany) following manufacturer’s instructions.
-
bioRxiv - Microbiology 2021Quote: ... samples were enzymatically analyzed using a D-Lactic Acid/L-Lactic Acid Enzymatic Bioanalysis UV-Test kit and a D-Glucose Enzymatic Bioanalysis UV-Test kit (Roche Diagnostics) with a modified manufacturer’s protocol to reduce total sample size to 300 uL ...
-
bioRxiv - Immunology 2020Quote: ... ATAC-Seq libraries were then purified using a QIAGEN PCR cleanup kit and quantified using KAPA library quantification kit (KAPA Biosystems, Roche) and sequenced on the Nextseq 550 platform.
-
bioRxiv - Synthetic Biology 2020Quote: ... the intracellular ATP were measured using the enzymatic assay kit (ATP Bioluminescence Asssay Kit HS II, Sigma No. 11699709001, Roche, Switzerland) after the cell disruption of the corynebacterial strains ...
-
bioRxiv - Immunology 2021Quote: ... ATAC-Seq libraries were then purified using a QIAGEN PCR cleanup kit and quantified using KAPA library quantification kit (KAPA Biosystems, Roche) and sequenced on the Nextseq 550 platform.
-
bioRxiv - Neuroscience 2022Quote: ... was then transferred to a MagNaPure 96 Processing Cartridge and RNA was purified using the MagNa Pure 96 with the kit Cellular RNA Large Volume Kit (05467535001) and using the protocol “RNA Tissue FF Standard LV 3.1” (Roche Life Science). Remaining lysate was stored for later analysis of ASO contend (see below).
-
bioRxiv - Immunology 2022Quote: ... mRNAseq libraries were constructed using KAPA RNA HyperPrep Kit with RiboErase (HMR) Globin in conjunction with the KAPA mRNA Capture Kit (Roche Sequencing). Libraries were sequenced on an Illumina NextSeq500 sequencer using Illumina NextSeq 500/550 High Output v23 (150 cycles ...
-
bioRxiv - Genetics 2023Quote: ... Immunoprecipitated chromatin was then indexed with the Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences 21024) and amplified with KAPA Library Amplification Kit (Roche KK2620), before paired-end next generation sequencing.