Labshake search
Citations for Roche :
1601 - 1650 of 2089 citations for Adenovirus Type 5 Particles CMV GFP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... cells were incubated in staining solution (1 M Tris-HCl pH 9.5, 5 M NaCl, 1 M MgCl2, NBT-BCIP, H2O) (Roche) in the dark for 10 min at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... 20 mM NaCl, 5 mM MgCl2, 0.5% NP-40, 1 mM DTT, and 1x cOmplete EDTA-free protease inhibitor cocktail [Roche]), and washed again in PBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... 20 mM NaCl, 5 mM MgCl2, 0.5% NP-40, 1 mM DTT, and 1x cOmplete EDTA-free protease inhibitor cocktail [Roche]), and washed again in PBS ...
-
bioRxiv - Bioengineering 2023Quote: Liver tissue was lysed using RIPA buffer (50 mM Tris [pH 7.5], 150 mM NaCl, 0.1% SDS, 1% NP-40, 0.5% sodium deoxycholate, cOmplete® Mini Protease Inhibitor Cocktail [Roche Diagnosis ...
-
bioRxiv - Systems Biology 2023Quote: ... Up to 5 ng plasma cfDNA (~0.5 mL plasma) was used for cfWGS library with Kapa HiFi Hotstart ReadyMix (Roche) in 11-13 cycles ...
-
bioRxiv - Plant Biology 2023Quote: ... 5% (vol/vol) glycerol and 20 mM imidazole (buffer A) supplemented with cOmplete EDTA-free protease inhibitor tablets (Roche) and lysed by sonication ...
-
bioRxiv - Molecular Biology 2023Quote: A 20% w/v homogenate was prepared with a piece of snap-frozen liver tissue in homogenization buffer (50 mM Tris-HCl [pH 7.4], 250 mM sucrose, 25 mM KCl, 5 mM MgCl2, 3mM imidazole, supplemented with protease inhibitors [Roche]). To remove nuclei and debris ...
-
bioRxiv - Genomics 2023Quote: ... Cells were centrifuged at 500 x g for 5 min at 4C and 500 uL 1X PBS + 1% BSA + DAPI + 0.5U/uL Protector RNAse Inhibitor (Roche) was added ...
-
bioRxiv - Biochemistry 2023Quote: ... Mitochondrial pellets were solubilized in lysis buffer with 1 % digitonin (25 mM HEPES pH 7.5, 150 mM NaCl, 5 % glycerol, 1 mM TCEP, supplemented with PhosStop (Roche), cOmplete protease inhibitor cocktail EDTA-free (Roche) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM EDTA, 20 mM HEPES, 0.2% NP-40, 10% glycerol, 400U Ribolock inhibitor, 1x protease/phosphatase inhibitor, Roche). Lysates were centrifuged (12000 rpm ...
-
bioRxiv - Cell Biology 2023Quote: ... 500 mM NaCl, 0.4% [wt/vol] SDS, 5 mM EDTA, 1 mM DTT and 1x cOmplete Protease inhibitor cocktail, Roche) for 20 min on a rocker at 4°C ...
-
bioRxiv - Genomics 2023Quote: ... resuspended in cold lysis buffer (10 mM Tris, pH 7.5, 10 mM NaCl, 5 mM MgCl2, 0.1 mM EGTA, Protease Inhibitor (Roche, 04693159001)) and incubated on ice for 10’ to isolate the cell nuclei ...
-
bioRxiv - Cell Biology 2023Quote: ... 50 mM NaCl, 5 mM EDTA, 50 mM Tris at pH 7.4 and cOmplete EDTA-free Protease Inhibitor Cocktail [Roche]). After agitation at 4°C for 30 min ...
-
bioRxiv - Immunology 2023Quote: ... These cells were cultured with healthy donor T cells (E:T ratio of 5:1) and different concentrations of Mosunetuzumab (Genentech, Roche): 0 ...
-
bioRxiv - Immunology 2023Quote: ... the cells were re-stimulated with 5 μg/mL CTH522 or 1 μg/mL phytohemagglutinin (PHA; Roche, cat.: 11249738001) as positive control ...
-
bioRxiv - Genomics 2023Quote: ... Library construction was performed on the 5 ng of pooled PCR amplicons using the KAPA HyperPrep Kit (Roche 07962363001) and NEXTFLEX Unique Dual Index Barcodes (7.5µM final concentration ...
-
bioRxiv - Biochemistry 2023Quote: Cell pellets were snap frozen in Eppendorf tubes and lysed with glass beads in 500 μl GFP pull-down buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...
-
bioRxiv - Biophysics 2022Quote: ... 5 EDTA and 1% Triton X-100) supplemented with cOmplete™ Mini-EDTA free protease inhibitor cocktail (Roche, Germany). Lysates were incubated 5 min on ice and centrifuged 10 min - 14000 x g at 4°C ...
-
bioRxiv - Cell Biology 2022Quote: ... The resulting cell pellet was resuspended in a lysis buffer of Buffer A + 0.5% Triton X-100 containing 5% protease inhibitor cocktail cOmplete (Roche, 118614500 ...
-
bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 95°C for 5 min in SDS lysis buffer (5% SDS in 100 mM triethylammonium bicarbonate pH 8.5, complete EDTA free protease inhibitor cocktail (Roche), phosphatase inhibitor cokatail-2 ...
-
bioRxiv - Neuroscience 2023Quote: ... cells and sections were processed for BrdU immunodetection following the manufacturer’s recommendations (5-Bromo-2′-deoxy- uridine Labelling and Detection Kit I, Roche). The slides were mounted for fluorescent microscopy in ProLong® Diamond Antifade Mountant with DAPI (Life Technologies).
-
bioRxiv - Developmental Biology 2023Quote: ... The slides were washed and detection was performed at pH 9.5 by incubating in nitro blue tetrazolium and 5-bromo-4-cholro-2-indoyl phosphate solution (Roche) as per manufacturer instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were washed 1x with PBS and lysed with lysis buffer (25 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001] ...
-
bioRxiv - Biochemistry 2023Quote: ... and individually transferred in 1.5 ml vials filled with 5 volumes (V/W) of ice-cold 25 mM Hepes pH=7.4 in PBS completed with protease (Mini, Roche) and phosphatase (Phos-stop ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were lysed in with glass beads in 500 µl SPOT PD buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% Glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were lysed in with glass beads in 500 µl SPOT PD buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% Glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free ...
-
bioRxiv - Immunology 2023Quote: ... 2019) by harvesting lung lobes into 5 ml HBSS with 40µl Liberase Tm (0.1 wU/ml, Roche, Cat# 5401127001) and 20µl DNAse 1 (10mg/ml ...
-
bioRxiv - Microbiology 2023Quote: ... Each 5 μl reaction contained 2.5 μl of LightCycler 480 SYBR Green I Master mix 2X (Roche Applied Science), 0.5 μl of each primer (300 nM final ...
-
bioRxiv - Microbiology 2023Quote: ... 0.5 mM EDTA, 0.1 % NP-40, 20 mM KCl, 150 mM NaCl, plus a protease inhibitor cocktail from Roche) and centrifuged for 2 minutes and 2,500 g at 4 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with 500 μl acid-washed glass beads in 500 μl GFP-Trap pulldown buffer (20 mM HEPES, pH 7.4, 150 mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...
-
bioRxiv - Developmental Biology 2023Quote: Lungs extracted from E14.5 or E18.5 embryos were minced and digested using collagenase I and dispase I (both 1 mg/mL, Roche) and incubated at 37°C for 5 min ...
-
bioRxiv - Biochemistry 2023Quote: ... under gravity flow, washed with PER2 buffer (1 x TBS pH 7.5, 1mM EDTA, 5% glycerol, EDTA-free protease inhibitors (Roche)) and eluted with 3x flag peptide in PER2 buffer ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and 1X Triton buffer (100mM Tris-HCl, pH8.8, 100mM NaCl, 5mM EDTA and 5% Triton-X) with protease inhibitors (11836170001, Roche) for 16 hours at 4° C ...
-
bioRxiv - Biochemistry 2023Quote: ... then the pellet was resuspended in 40 ml binding buffer (50 mM NaPi, 300 mM NaCl, 5 mM imidazole, pH 7.5) containing protease inhibitor (Roche cOmplete EDTA-free protease inhibitor cocktail ...
-
bioRxiv - Cell Biology 2024Quote: Cells or tissues were washed with PBS and lysed in an appropriate amount of protein lysis buffer (TritonX100 -1%, Tris 7.4 -50mM, EDTA -5mM, NaCl -150mM, and glycerol -5%) containing Protease Inhibitor Cocktail (Roche). We used Phosphatase Inhibitor Cocktail (Millipore ...
-
bioRxiv - Microbiology 2023Quote: ... and resuspended in lysis buffer (50 mM PBS pH 7.4, 1 mM EDTA, 5% glycerol, 1 mM PMSF and protease inhibitors from Roche). Cell lysis and protein extraction was achieved through mechanical lysis using glass beads and Precellys®24 Homogenizer (3 cycles of 30 seconds at 5500 rpm ...
-
bioRxiv - Biochemistry 2023Quote: ... The pellet was thawed in two volumes of lysis buffer (3M NaCl, 100mM Tris pH7,5, 10% Glycerol, 0.5mM EDTA, 5 mM β-mercaptoethanol, protease inhibitors (Roche)) and sonicated on ice (10 pulses of 10 seconds) ...
-
bioRxiv - Cell Biology 2023Quote: ... 5 % glycerol and 0.5 mM TCEP supplemented with 5 Units/mL Benzonase and an EDTA-free protease inhibitor cocktail (Roche)) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The pellet was thawed in an equal volume of lysis buffer (1M NaCl, 25mM Tris pH7,5, 5% Glycerol,1mM DTT, protease inhibitors (Roche)) and sonicated on ice (10 pulses of 10 seconds) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The pellet was thawed in two volumes of lysis buffer (3M NaCl, 100mM Tris pH7,5, 10% Glycerol, 0.5mM EDTA, 5 mM β-mercaptoethanol, protease inhibitors (Roche)) and sonicated on ice (10 pulses of 10 seconds) ...
-
bioRxiv - Physiology 2024Quote: ... Sections were dried and denatured for 10 min at 80°C in hybridization buffer (0.1M Tris, pH 7.2, 25mM MgCl2, 70% formamide [Sigma-Aldrich, Saint Louis, MO], 5% blocking reagent [Roche] with 1.0 μg/mL of Cy-3-labeled telomere-specific [CCCTAA] peptide nucleic acid [PNA] probe [TelC-Cy3 ...
-
bioRxiv - Plant Biology 2024Quote: ... 50 mM Tris-HCl [pH 7.5], 150mM NaCl, 1 mM EDTA, 0.2% IGEPAL® CA-630, 0.5 mM DTT, 1X protease inhibitor cocktail [Roche]) in a 3:1 v/w ratio ...
-
bioRxiv - Cancer Biology 2024Quote: ... On the following day 10 µl MTT labeling reagent (5 mg/ml) of the Cell Proliferation Kit I (Roche) was added and cells were incubated at 37 °C ...
-
Conserved Cis-Acting Range Extender Element Mediates Extreme Long-Range Enhancer Activity in MammalsbioRxiv - Genomics 2024Quote: ... embryos were with PBT (x3, 15mins) and incubated in prehybridization buffer (50% deionized formamide, 5× SSC, pH 4.5, 2% Roche Blocking Reagent ...
-
bioRxiv - Developmental Biology 2024Quote: ... Larvae were placed in 1.5mL microcentrifuge tubes containing 1mL of 5% cOmpleteTM EDTA-free protease inhibitor cocktail (PIC) (Roche Diagnostics Corporation ...
-
bioRxiv - Cell Biology 2024Quote: ... A probe binding the left flank of the acbd4/5 locus was generated using DIG probe labelling mix (Roche) as per the manufacturer’s instruction (primers acbd4/5_LF_fw and acbd4/5_LF_rv ...
-
bioRxiv - Microbiology 2024Quote: ... Cell pellets were resuspended in cold Buffer A-5 (20mM Tris-HCl, pH 7.9, 500mM NaCl, 5mM Imidazole) with an EDTA-free protease inhibitor tablet (Roche) and kept on ice ...
-
bioRxiv - Microbiology 2024Quote: ... each sample was normalized to 3.5 ng in 5 µL water before generating libraries using a 1:10 Kapa HyperPlus (Roche) miniaturized protocol with barcoded indices as previously described82 ...
-
bioRxiv - Cancer Biology 2021Quote: ... at 95°C for 5 min (90°C for 15 sec, 60°C for 60 sec) × 50 cycles (Light/Cycler Nano, Roche). The primer sets used in this study were described in the supplemental Table 1.
-
bioRxiv - Microbiology 2020Quote: ... coli cells were resuspended in 100 mL of cold lysis buffer (50 mM HEPES pH 8, 175 mM NaCl, 5 % glycerol, 1 mM EDTA and 2 tablets of protease inhibitors by Roche). The bacterial solution was then incubated at room temperature (RT ...