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Citations for Roche :
1551 - 1600 of 1685 citations for Arsenazo I Trisodium Salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... were lysed in deionized water containing 0.1 µg ml-1 RNase A (Roth, Karlsruhe, Germany) and 0.1 µg ml-1 DNase I (Roche, Mannheim, Germany), respectively ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative real-time PCR was performed in duplicate using 1:20 or 1:5 dilutions of the cDNA and SYBR Green I master mix (Roche) with a LightCycler 480 II (Roche ...
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bioRxiv - Immunology 2024Quote: ... Whole lymph nodes or chopped spleens were then transferred into 15 ml conical tubes containing 2 ml digestion buffer (RPMI containing 2% FBS, 0.1mg/ml DNAse I (Roche, #10104159001), 0.2 m/ml collagenase P (Roche ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Purified DNA was used for subsequent qPCR reactions using SYBR Green I Master Mix on a LightCycler 480 System (Roche). Enrichment was calculated using the following formula ...
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bioRxiv - Developmental Biology 2024Quote: ... The real-time PCR was performed with Roche LightCycler 480 SYBR GREEN I Master mix on a LightCycler 480 system (Roche). Relative gene expression abundance between samples was calculated using the CT method and Gapdh as an internal control ...
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bioRxiv - Physiology 2024Quote: ... The process was halted by adding 100uL fetal bovine serum (FBS; Anprotec, Bruckberg, Germany) and 100μL DNase I (0.5 mg/mL; Roche, Basel, Switzerland) after 15 minutes (min ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated in RPMI medium with a digestion solution (1mg/mL collagenase IV from Sigma - C5138-100mg - and 0.2 mg/mL DNase I from Roche - 11284932001) at 37°C for 30 min ...
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bioRxiv - Immunology 2024Quote: ... or digested in 0.2 mg/mL Collagenase IV (sigma, Cat# C5138) and 0.05 mg/mL DNAse I (Roche, Cat# 10104159001) in RPMI 1640 medium for 1 hour at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... lung tissue was minced and incubated in 3 mL of serum-free media containing 0.5 mg/mL DNase I (Roche, 10104159001) and 1 mg/mL collagenase type IV (Worthington ...
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bioRxiv - Bioengineering 2024Quote: ... sulfanilamide (Reagent I) and N-(1-Naphthyl)-Ethylenediamine in hydrochloric acid (Reagent II) from the “Nitrite/Nitrate Colorimetric Test” (Roche) kit (#11746081001 ...
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bioRxiv - Biochemistry 2024Quote: ... The cell lysate was incubated for 30 min at 4 °C with 0.1 mg/mL DNAse I from bovine pancreas (Roche 11284932001) and 10 mM MgSO4 ...
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bioRxiv - Cancer Biology 2024Quote: ... The reactions were performed in duplicate in 12.5μl of LightCycler® 480 SYBR Green I Master (Roche Diagnostics, cat. 0470751600), 2.5μl of forward and reverse 10μM primers ...
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bioRxiv - Cell Biology 2024Quote: ... the digestion with 3 mg/mL type I collagenase was performed in parallel to and compared with 3 mg/mL type I collagenase + 4 mg/mL type II Dispase (Roche), 250 μg/mL Liberase DL (Roche ...
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bioRxiv - Microbiology 2020Quote: ... DNA was first eluted from the column in 100 µL DNA elution buffer and subsequently the column was treated with recombinant DNase I (20 units/100 µL; Roche Diagnostics) for 30 min at 37°C and finally RNA was eluted in 50 µL nuclease free water ...
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bioRxiv - Molecular Biology 2021Quote: ... Then the cDNA samples were amplified using LightCycler® 480 SYBR® Green 2x PCR Master Mix I (Roche, Cat# 04887352001) and 0.6 μM of forward and reverse primers with a LightCycler® 480 (Roche) ...
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bioRxiv - Immunology 2021Quote: ... was added to the PBMCs for 5 minutes before transferring drop-wise to a 10ml volume of warmed media (RPMI/20%FBS) containing 200U/ml of DNase I (Roche, Switzerland). After 10 minutes at room temperature ...
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bioRxiv - Genomics 2021Quote: ... We calculated a signal to noise ratio for final feasibility test libraries using LightCycler® 480 SYBR Green I Master Mix (Roche) along with custom primers for the promoter of human GAPDH and a heterochromatic gene desert region (Table S12) ...
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bioRxiv - Immunology 2021Quote: ... which were subsequently incubated with digestion buffer (IMDM supplemented with 2% FBS, 1 mg/mL Collagenase D [Roche], 2 U/mL DNase I [Life Technologies] and Dispase II [Roche]). The digested brain parenchyma ...
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bioRxiv - Cancer Biology 2020Quote: ... using primers 3PFK2fo2 and iPFK2re6 as well as the LightCycler FastStart DNA Master Plus Set SYBR Green I Kit (Roche Diagnostics) according to the instruction manual.
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bioRxiv - Cancer Biology 2020Quote: ... Il1r1/IL1R1 and Gapdh/GAPDH qPCR was performed using specific primers and Lightcycler 480 Sybr Green I Master (Roche; Mannheim, Germany) in a Lightcycler 480 II (Roche Diagnostics) ...
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bioRxiv - Microbiology 2021Quote: ... Phages and bacteria from the heat-inactivated samples were quantified using qPCR using SYBR Green I Master with the Lightcycler® 480 (Roche). qPCR primers and cycling protocols for E ...
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bioRxiv - Cell Biology 2022Quote: 3T3-L1 adipocytes were washed with ice-cold PBS and harvested in ice-cold HES-I buffer (20 mM HEPES, pH 7.4, 1 mM EDTA, 250 mM sucrose containing protease inhibitors mixture (Roche Applied Science)) ...
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bioRxiv - Immunology 2022Quote: Spleens or lymph nodes were harvested and submitted to enzymatic digestion for 25 minutes at 37°C with Collagenase IV (Wortington biochemicals) and DNAse I (Roche Diagnostics). Organs were then mechanically digested and passed over 100µm cell strainers (Corning) ...
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bioRxiv - Microbiology 2022Quote: ... South Korea) was made in 10 ml per brain of digestion cocktail containing 0.5 mg/ml DNase I (Roche, Basel, Switzerland) and 1 mg/ml Collagenase A (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... 1 µl of the newly synthesized cDNA was then used to perform quantitative RT-PCR was in LightCycler 480 SYBR Green I Master (Roche, 04707516001) according to the protocol below on a LightCycler 480 Instrument or the QuantStudio 6 Flex instrument (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was quantified by Real-Time PCR on Light Cycler II using Light Cycler 480 SYBR Green I Master (Roche, 04887352001).
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bioRxiv - Genetics 2022Quote: ... Hybridization was performed as described in the Instruction Manual of the DIG High Prime DNA Labeling and Detection Starter Kit I (Roche Diagnostics). Washing conditions was performed according to Huang et al (Huang et al ...
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bioRxiv - Developmental Biology 2022Quote: ... 50mM Tris-HCl [pH 7.2], 250mM NaCl, 2mM EDTA, phosphatase inhibitor mixture I and II [Calbiochem] and one tablet of Minicomplete [Roche] per 10ml) on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... HCXII-0155) was incubated with neutrophils that were pre-treated with vehicle or DNase I (10U/ml, Dornase alpha, Roche, Germany) for 1h ...
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bioRxiv - Plant Biology 2020Quote: ... in a total volume of 6 μL containing 0.5 mM of each specific primer and 3 μl of SYBR Green I Master Mix (Roche Applied Science). The second derivative maximum method was used to determine Cp values and PCR efficiencies were determined using LinRegPCR software (http://LinRegPCR.nl) ...
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bioRxiv - Developmental Biology 2021Quote: ... was carried out using 10 ng of cDNA and 100 nM of each primer pair with a Roche LightCycler 480 machine and LightCycler 480 SYBR Green I Master 2X (Roche, 04887352001). The PCR program was ...
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bioRxiv - Cell Biology 2020Quote: ... 1μl of cDNA was used in the QPCR reaction with the LightCycler® 480 SYBR Green I Master (catalogue number 04707516001) from Roche and according to manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was passed on a MicroSpin™ G-50 columns (Cytiva) and qPCR was performed using SYBR Green I Master Mix (Roche). A volume of cDNA corresponding to 20 ng of initial RNA was used ...
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bioRxiv - Physiology 2021Quote: ... DNase treatment was performed on 300 ng RNA prior to Reverse Transcription Polymerase Chain Reaction (RT-PCR) using RNAse-free DNAse I (Roche, # 04716728001) at 37°C for 10 min ...
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bioRxiv - Plant Biology 2021Quote: ... and NADP-ME3 (Zm00004b015828) were quantified via qRT-PCR using a Roche Light Cycler 480 II using Roche SYBR green I (Roche 04707516001). The delta cT method was used to quantify relative gene expression of PEPCK1 and NADP-ME3 over time ...
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bioRxiv - Immunology 2021Quote: ... were thawed by washing twice in pre-warmed RPMI1640 medium supplemented with 20% FCS and 10 ng/mL DNAse I (Roche, 11284932001). Thawed CAR T cells were rested in cytokine-free complete medium (RPMI1640 medium containing 10% FCS and 1% penicillin-streptomycin ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 volume of nuclei suspension was mixed with 1 volume of DNase I cocktail (NWB supplemented with CaCl2 2 mM, BSA 100 μg/ml, and twice the indicated concentration of DNase I (Roche 10104159001) and incubated for 8 minutes at RT ...
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bioRxiv - Cancer Biology 2022Quote: Cancer transplantation was performed as described in the section “spatial transcriptomics.” The obtained livers were minced and dissociated in RPMI1640 containing DNase I (100 μg/ml: Roche, Switzerland) and collagenase IV (1 mg/ml ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were resuspended in twice their volume of buffer A (50 mM HEPES pH 7.5, 500 mM KCl) with 5 mM MgCl2 and DNase I (Roche, Basel, Switzerland). The cell suspension was treated with a Sonopuls GM200 sonicator (BANDELIN electronic GmbH & Co ...
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bioRxiv - Developmental Biology 2024Quote: Immune cell FACS was performed from ear skin from which cells were isolated using DNAse I 40 µg/ml (Roche, 11284932001) and 20 U/ml Collagenase Type I Worthington (LS0004194 ...
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bioRxiv - Cancer Biology 2024Quote: ... qPCR was performed on genomic DNA from all 69 tumor sections and the patient’s blood using the LightCycler 480 SYBR Green I master mix and LightCycler 480 qPCR machine according to the manufacturer’s recommendations (Roche, Nutley, NJ). Measurements were triplicated and data were analyzed using the standard curve method ...
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bioRxiv - Microbiology 2024Quote: ... cDNAs were diluted to 1 ng/ µL and used for qPCR analysis in a 10 µL reaction mix containing 5 µL of LightCycler® 480 SYBR Green I Master mix (Roche), 300 nM of each primer ...
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bioRxiv - Biochemistry 2024Quote: ... 5 mM β-mercaptoethanol and 20 μM GDP supplemented with DNase I (10 μg/ml) and cOmplete protease inhibitor cocktail tablets (Roche). Cells were mechanically disrupted using a Microfluidizer (Microfluidics International Corporation ...
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bioRxiv - Biochemistry 2024Quote: ... Quantitative PCR was performed with 2 µL of cDNA as a template in a total volume of 20 µL containing LightCycler FastStart DNA MasterPlus SYBR Green I (Roche, USA) and 0.5 µM primer ...
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bioRxiv - Cancer Biology 2024Quote: KPC-4662 tumors from sedentary and exercised mice were harvested and immediately digested in HBSS with DNase I (100 µg/mL; Roche, 04716728001) and collagenase type II (10mg/ml ...
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bioRxiv - Microbiology 2023Quote: ... South Korea) was made in 10 ml per brain of digestion cocktail containing 0.5 mg/ml DNase I (Roche, Basel, Switzerland) and 1 mg/ml Collagenase A (Roche ...
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bioRxiv - Immunology 2023Quote: ... brains and spinal cords were cut into pieces and digested for 30 min at 37 °C with DNase I (Sigma-Aldrich or Roche) at a concentration of 1 mg/ml and collagenase D (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Southern blotting was conducted to confirm the correct deletion using the digoxigenin (DIG) high prime DNA labeling and detection starter Kit I (11745832910 Roche Germany). The Myb gene complementation vectors were constructed by cloning the entire length of the target gene with the native promoter region (about 1.5-kb ...
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bioRxiv - Biochemistry 2023Quote: ... 2008)) was performed for each sample in biological triplicates by use of LightCycler® 480 SYBR Green I Master mix (Roche) and evaluated by LightCycler® 480 II cycler (Roche ...
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bioRxiv - Plant Biology 2023Quote: ... Expression of mRNA and pre-mRNA was then assayed by semi-quantitative reverse-transcription PCR (qRT-PCR) on a Roche LightCycler480 using SYBR Green I (Roche Diagnostics). Raw fluorescence data were analysed using LinRegPCR to perform background subtraction ...