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Citations for Roche :
1401 - 1450 of 1685 citations for Arsenazo I Trisodium Salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR reaction was prepared using a LightCycler® 480 SYBR Green I Master kit (Roche Diagnostics, Mannheim, Germany) and the PCR was performed with a LightCycler® 480 Instrument II (384-well ...
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bioRxiv - Immunology 2020Quote: ... minced with scissors and digested at 37°C for 20-30 minutes in digestion buffer (0.52 U/ml Liberase TM [Roche] and 60 U/ml DNase I [Roche] in RPMI 1640 [Cellgro] with 5% fetal bovine serum) ...
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bioRxiv - Immunology 2020Quote: ... fluorogenic trials were performed using the reagent Light Cycler 480 SYBR Green I Master (Roche Applied Science, Mannheim, Germany). Fluorescence was detected in the equipment Light Cycler 480 Instrument (Roche Applied Science ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative PCR reactions were performed in a total volume of 10 μL of SYBR Green master mix I (Roche) in 384-wells plates on a Lightcycler LC480 apparatus (Roche ...
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bioRxiv - Immunology 2020Quote: DNase treatment was performed prior to Reverse transcription polymerase chain reaction (RT-PCR) using RNAse-free DNase I (Roche) at 37°C for 10 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative gene expression analysis was performed using the LightCycler® 480 SYBR Green I Master (Roche; Woerden, The Netherlands) and measured using the LightCycler® 480 Instrument II (Roche) ...
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bioRxiv - Neuroscience 2021Quote: The tissue was dissociated in 300 µL of 0.25% trypsin-EDTA solution supplemented with 10 U/mL recombinant DNase I (Roche) for 15 minutes at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... 20 mM imidazole and 5 mM β-mercaptoethanol (BME)) and supplemented with DNase I and protease inhibitor cocktail (Roche). Cells were disrupted by Emulsiflex C3 (Avestin ...
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bioRxiv - Immunology 2021Quote: ... 1% penicillin-streptomycin, 1mM sodium pyruvate, 0.1% β-mercaptoethanol, 25mM HEPES, 150μg/ml DNase I [Sigma-Aldrich], 0.5mg/ml Liberase [Roche] and then slow spun at 50xg for 5 mins (11) ...
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Physiological Substrates and Ontogeny-Specific Expression of the Ubiquitin Ligases MARCH1 and MARCH8bioRxiv - Immunology 2021Quote: ... Whole single cell suspensions from spleen and subcutaneous LN (axillary and inguinal) were generated by spleen digestion with 0.1 % DNase I (Roche) and 1 mg/ml collagenase type III (Worthington ...
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bioRxiv - Developmental Biology 2020Quote: ... genomic DNA was digested on column with the addition of an extra 1μl of RNase-free DNase I (Roche) to ensure complete digestion ...
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bioRxiv - Genomics 2020Quote: ... Quantitative PCR (qPCR) were performed using LightCycler 480 SYBR Green I Master mix and real-time PCR instrument (Roche). PCR conditions were 95 ◦C for 5 min followed by 45 cycles of 15 s at 95 ◦C ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 μl of SEC samples were either pre-treated for 40 min at 37 °C with DNase I (Roche) at a concentration of 1 IU/μl ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were first mechanically dissociated with a scalpel into approximately 1-3 mm pieces and enzymatically digested in RPMI-1640 medium (Cytiva) containing 0.25 mg/ml DNase I (Roche) and 0.25 mg/ml Collagenase (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... the quantification of ABCB1 copy number was performed with LightCycler 480 SYBR Green I Master mix (Roche Diagnostics, 04707516001). ABCB1 genomic amplification primer sequences are listed in Sup ...
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bioRxiv - Immunology 2023Quote: ... Aortas were manually homogenized and digested for 20 minutes at 37°C in RPMI with 1 mg/mL of collagenase A and collagenase B and 0.1 mg/mL DNAse I (Roche). Kidneys were homogenized using the Miltenyi gentleMACS Dissociator and digested for 20 minutes at 37°C in RPMI with 2 mg/mL collagenase D (Roche ...
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bioRxiv - Genetics 2023Quote: ... incubated the islets for 10 min at 37°C in 2 mL PBS with 4U Dispase I (Roche Diagnostics) / 2U DNase I (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: Frozen cells were thawed and suspended in 50 mM Tris-HCl (pH 7.5) supplemented with a few crystals of DNase I (Roche) and protease inhibitors (PI ...
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bioRxiv - Cancer Biology 2023Quote: ... The total RT-qPCR reaction volume was 1.5⍰μL and consisted of 1⍰μL of LightCycler 480 SYBR Green I Master Mix (Roche) containing 0.5⍰μM of PCR primer (Eurogenetec ...
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bioRxiv - Microbiology 2023Quote: ... using 100 nL containing (final concentration) 1 × Light Cycler 480 SYBR® Green I Master Mix (Roche Inc., USA), nuclease free PCR-grade water ...
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bioRxiv - Microbiology 2023Quote: ... Each 20 µl qPCR consisted of 10 µl 2× Light Cycle 480 SYBR Green I Master (Roche Applied Sciences), 1 µM each primer ...
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bioRxiv - Cancer Biology 2024Quote: ... Total volume of 20 μl reaction mix contained 10 μl LightCycler® 480 SYBR Green I Master Mix (Roche), 2 μl 2.5 mM primer (F+R) ...
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bioRxiv - Neuroscience 2022Quote: ... in a 25 µl kinase assay buffer I (Signal-Chem, K01-09) supplemented with cOmplete protease inhibitor cocktail (Roche) and phosSTOP (Roche) ...
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bioRxiv - Immunology 2022Quote: ... and lung were further digested in RPMI + 50U/mL DNase I + 1mg/mL hyaluronidase + 1mg/mL collagenase D (Roche) on a shaker at 220rpm for 45 minutes at 37°C ...
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bioRxiv - Biophysics 2022Quote: ... The transcription mixture was incubated at 37 °C for 4 h and treated with 50U/mL DNAse I (Roche) at 37 °C for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... and PD-L1 was performed on 2.5 ng of RNA using the LightCycler EvoScript RNA SYBR Green I Master (Roche) and the LightCycler 480 Instrument II (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... 5-diphenyl tetrazolium bromide (MTT) in accordance with the manufacturer’s recommendations (Cell Proliferation Kit I; Roche Diagnostics, Basel, Switzerland). In brief ...
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bioRxiv - Neuroscience 2024Quote: ... Cerebral cortex was dissociated by trituration in DMEM/F12 containing 10% FBS and 20 µg/ml DNase I (Roche) using a fire-polished Pasteur pipette ...
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bioRxiv - Biochemistry 2023Quote: ... 10 s off cycle using a total of 30 minutes) in the presence of DNase I (Roche, Basel, CH) and cOmplete EDTA-free protease inhibitor (Roche ...
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bioRxiv - Cell Biology 2023Quote: MTT activity was determined using the Cell Proliferation Kit I according to the recommendations of the manufacturer (Roche, Spain). Optical density was determined at 575[nm with a reference wavelength of 690[nm using a Varioskan Flash spectrophotometer (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Bones were cut into small fragments using scissors in a dissociating media containing 10 U/ml DNase I and Collagenase P (1.2 mg/ml, 11213865001, Roche). Bones and BM were digested at 37°C for 20 min under agitation ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 μg DNase I from bovine pancreas) and a single dissolved cOmplete EDTA-free Protease Inhibitor Cocktail tablet (Roche). The pellet was resuspended using a Biospec Tissue Tearor (Dremel ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was carried out in technical triplicates using SYBR Green I Master Mix and Lightcycler 480 apparatus (Roche); negative control was represented by cDNA synthesis samples performed in the absence of the reverse transcriptase enzyme ...
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bioRxiv - Immunology 2022Quote: ... and the lamina propria was minced and digested in RPMI medium containing 2.5 mg/ml Liberase TL and 20 U/ml DNase I (both from Roche) at 37°C for 1 h ...
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bioRxiv - Biophysics 2022Quote: ... Frozen bacteria containing full-length or ΔUBLΔUBA UBQLN constructs were resuspended in Buffer A (50 mM Tris pH 8, 1 mM MgCl2, 1 mM PMSF, and 0.2 mg/mL DNase I and Roche Complete Mini protease inhibitor cocktail) ...
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bioRxiv - Immunology 2022Quote: ... RT-qPCR was carried out using LightCycler 480 SYBR green I master chemistry and a LightCycler 480 machine (Roche). All samples were measured in triplicates ...
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bioRxiv - Neuroscience 2023Quote: ... cells and sections were processed for BrdU immunodetection following the manufacturer’s recommendations (5-Bromo-2′-deoxy- uridine Labelling and Detection Kit I, Roche). The slides were mounted for fluorescent microscopy in ProLong® Diamond Antifade Mountant with DAPI (Life Technologies).
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bioRxiv - Microbiology 2023Quote: ... Each 5 μl reaction contained 2.5 μl of LightCycler 480 SYBR Green I Master mix 2X (Roche Applied Science), 0.5 μl of each primer (300 nM final ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... Quantitative PCRs were performed using the KAPA SYBR qPCR Master Mix (SYBR Green I dye chemistry) (KAPA BIOSYSTEMS, KK4618) and the CFX connect apparatus (Bio-Rad) ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... Quantitative PCRs were performed using the KAPA SYBR qPCR Master Mix (SYBR Green I dye chemistry) (KAPA BIOSYSTEMS, KK4618) and the CFX connect apparatus (Bio-Rad) ...
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Impaired yolk sac NAD metabolism disrupts murine embryogenesis with relevance to human birth defectsbioRxiv - Developmental Biology 2024Quote: ... using the primers detailed in Table S7 and LightCycler 480 SYBR Green I Master mix (04887352001; Roche Holding AG). Relative expression was calculated using the ΔCt method ...
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bioRxiv - Microbiology 2024Quote: ... the nucleic acid pellet was resuspended in 38μL of DNase buffer and 2μL of 10U/μL DNase I (Roche, #04716728001), gently mixed ...
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bioRxiv - Genomics 2024Quote: ... and 36B4 standard were calculated via Real-Time PCR method using the SYBR Green I Master mix (Roche, Germany), and telomere and 36B4 primers ...
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bioRxiv - Cancer Biology 2024Quote: ... On the following day 10 µl MTT labeling reagent (5 mg/ml) of the Cell Proliferation Kit I (Roche) was added and cells were incubated at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... cDNA (10 ng per sample) was then analyzed by qRT-PCR using LightCycler 480 SYBR Green I Master (Roche). Reactions were performed in a 384-well format on a QuantStudio 7 Pro at Harvard Medical School’s ICCB-Longwood Screening Facility ...
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bioRxiv - Immunology 2024Quote: ... cDC were prepared from auricular LNs by digesting with 100µg/mL Liberase TL and 100µg/mL DNase I (both Roche, Germany) for 20 minutes at 37°C before passing through a 70µm cell strainer ...
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bioRxiv - Immunology 2024Quote: ... resuspended in RPMI-1640 (Fisher) supplemented with 10% Fetal Bovine Plasma (FBS) and 20 U/mL deoxyribonuclease I (Roche), concentrated by centrifugation (300 x g for 15 min at 20°C) ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative polymerase chain reactions (qPCRs) were performed using LIGHTCYCLER 480 SYBR GREEN I Master Mix (Roche, Indianapolis, IN, USA) in a Bio-Rad CFX384 Touch Real-Time PCR Detection System with 10 µL reaction volume ...
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bioRxiv - Microbiology 2024Quote: ... was used to amplify cDNAs in a mix of 1x Lightcycler® 480 SYBR Green I Master Mix (Roche) and 0.2 µM of a series of sequence-specific primer pairs (see Table 1 for detailed target sequences) ...
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bioRxiv - Cancer Biology 2024Quote: ... A mixture comprising 2.5 µL of both forward and reverse primer dilutions (0.5 µM each) and 5 µL of LC480 SYBR Green I Master mix (Roche) was prepared per well in a 96-well plate format ...