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Citations for Roche :
1501 - 1550 of 9230 citations for Monoisodecyl Phthalate 100 Ug Ml In Mtbe Unlabeled Mono 3 7 Dimethyl 1 Octyl Phthalate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... NaCl 150 mM supplemented with 1% Triton X-100 in the presence of protease inhibitors (Complete, Roche Diagnostics GmbH). Fusion proteins were purified on glutathione-Sepharose (Cytiva ...
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bioRxiv - Genomics 2023Quote: ... and the pellet resuspended in 100 µl of ice-cold RIPA lysis buffer containing 1× protease inhibitor cocktail (Roche), incubated for 30 min on ice ...
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bioRxiv - Biophysics 2022Quote: ... 5 EDTA and 1% Triton X-100) supplemented with cOmplete™ Mini-EDTA free protease inhibitor cocktail (Roche, Germany). Lysates were incubated 5 min on ice and centrifuged 10 min - 14000 x g at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed 1x with PBS and lysed with lysis buffer (25 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001] ...
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bioRxiv - Plant Biology 2023Quote: ... 5% glycerol, 50mM KCl, 5 mM EDTA, 0.1% Triton X-100, 5 mM DTT and 1 × protease inhibitor cocktail [Roche]). The homogenized lysates were incubated at 4 °C for 1 hour for western blot analysis of eIFiso4G1 or overnight for mass spectrometry analyses with 50 µl of prewashed immobilized γ-aminophenyl-m7GTP (C10-spacer)-agarose beads (Jena Bioscience) ...
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bioRxiv - Cell Biology 2023Quote: Cells were lysed in immunoprecipitation (IP) buffer (1× PBS, 0.1% Triton X-100, and cOmplete EDTA-free protease inhibitor cocktail [Roche]) and then disrupted by three pulses of sonication for 5 s ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.0, 1 mM EDTA, 10 mM DTT, 0.05% [v/v] Triton X-100 and 1x Protease Inhibitor Cocktail [Roche]). Crude leaf extracts were incubated for 2 h at 4 °C on a turning wheel and centrifuged at 16.900 g for 5 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Mixed-stage transgenic worms expressing ectopic GFP::EGO-1 were collected and resuspended in equal volumes of 2x lysis buffer (50 mM Tris-HCl [pH 8.0], 300 mM NaCl, 10% glycerol, 1% Triton X-100, Roche ®cOmplete EDTA-Free Protease Inhibitor Cocktail ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were suspended in ice cold lysis buffer (150 mM NaCl, 1% Triton X-100, 50 mM Tris-HCl pH 8.0) supplemented with protease inhibitors (Roche) and incubated on ice for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... washed with 1 x PBS and resuspended in 100 μl of SoTE buffer (10 mM Tris-HCl pH 8, 1 mM EDTA, 0.15 % Triton X-100, protease inhibitor cocktail (Roche)) ...
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bioRxiv - Genetics 2023Quote: VSMCs and myofibroblasts were scraped in phosphate-buffered saline (PBS) supplemented with protease inhibitor cocktail (1:100, Roche, 11836145001) and phosphatase inhibitor cocktail (1:100 ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were washed twice in ice-cold PBS and resuspended in 600 uL NLB (1xPBS, 300 mM NaCl, 1% TritonX-100, 0.1% Tween-20) freshly supplemented with 1x Complete protease inhibitors (Roche), and 1x PhosSTOP (4906845001 ...
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bioRxiv - Cell Biology 2024Quote: ... Pelleted cells were resuspended in Lysis buffer (50mM Tris pH 7.4, 150mM NaCl, 5mM MgCl2 1% Triton X-100, protease inhibitors (Roche Complete Inhibitor cocktail ...
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bioRxiv - Microbiology 2023Quote: ... and 0.1% Triton X-100 supplemented with 1 mM PMSF and one Complete Protease Inhibitor tablet (EDTA-free; Roche), and then centrifuged at 13,000 × g for 10 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were detached using cold PBS and lysed in 350 µl lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1% Triton X-100) containing protease inhibitor (Roche) for at least 20 min on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Developmental Biology 2020Quote: ... 5% Normal Goat Serum) for 3 h at room temperature and then incubated overnight with alkaline phosphatase-conjugated anti-DIG Fab fragments (Roche, 1: 2,000) at 4°C on a shaking platform ...
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bioRxiv - Neuroscience 2020Quote: ... 1996) on 16 µm cryosections at post-natal day 3 (P3) using anti-digoxigenin antibody (Sheep polyclonal, 1:1000, Roche, 11093274910, RRID:AB_514497). Two days exposure to alkaline phosphatase (AP ...
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bioRxiv - Physiology 2021Quote: ... a 6.7 kb DNA fragment starting 1 kb upstream of murine Gnrhr exon 3 was amplified by PCR using the Expand Long Template PCR System (Roche, Basel, Switzerland) from 129SvEv genomic DNA using primers incorporating 5’ XmaI and 3’ NotI restriction enzyme sites (Table 1) ...
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bioRxiv - Immunology 2021Quote: ... was incubated with 50μl MTT (sodium 3′-[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis (4-methoxy-6-nitro) benzene sulfonic acid hydrate) labeling reagent (Roche Life Science, USA) to each well ...
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bioRxiv - Genetics 2021Quote: ... and cell pellets were then resuspended in 5ml of buffer 1 (10 mM Tris-HCl at pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Roche Complete protease inhibitor), incubated for 20 min at 4°C followed by a centrifugation step ...
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bioRxiv - Genetics 2021Quote: ... 30 million equivalent cell pellets were then resuspended in 5ml of buffer 1 (10 mM Tris-HCl at pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Roche Complete protease inhibitor), incubated for 20 min at 4°C and collected cells by a centrifugation step ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were lysed in 50 µl of lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40, 1× Roche Complete protease inhibitors cocktail) by pipetting up and down ...
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bioRxiv - Developmental Biology 2022Quote: ... The libraries were indexed using KAPA Unique Dual-Indexed Adapter Kit at 7 µM (KK8727, Roche Life Science) and 7 cycles of PCR library amplification were used ...
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bioRxiv - Microbiology 2019Quote: ... cells were harvested at 0 to 7 DPI in RIPA buffer containing protease and phosphatase inhibitor cocktails (Roche). Protein concentrations were determined by bicinchoninic acid (BCA ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA extracted from the parasites at passages 7 and 20 were digested with 5 U Rsa1 (Roche) at 37 °C overnight ...
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bioRxiv - Physiology 2024Quote: ... from two bees were pooled and homogenized in 250 µL of phosphate-buffered saline (7 mM NaCl, 2.7 mM KCl, and 10 mM PO4, pH 7.4) containing cOmplete protease inhibitors (Roche) at kept on ice until further processing ...
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bioRxiv - Cancer Biology 2022Quote: ... using the gRNA_enrichment1_fw (5’-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCTTGTG-GAAAGGACGAAACACCG-3’) and gRNA_enrichment1_rv (5’-CTACACGAC-GCTCTTCCGATCT-3’) primers and the 2x KAPA HiFi HotStart Ready Mix (Roche, Cat. No. KK2601). In a subsequent PCR ...
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bioRxiv - Microbiology 2022Quote: The V4 hypervariable region of the 16S rRNA gene was amplified with the primer pair 515F (5′-GTGCCAGCMGCCGCGGTAA-3′) and 806R (5′-GGACTACHVGGGTWTCTAAT-3) on a Fast Start High Fidelity PCR System (Roche, PQ) using the following conditions ...
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bioRxiv - Neuroscience 2024Quote: ... 5’-ATGGGCACCCAAAACAACAGT-3’ and 5’-GCGGCAGCACATATCCAAAAA-3’) was PCR amplified with a fast-cycling polymerase (KAPA2G Fast HotStart PCR kit, KAPA Biosystems) and a subsequent gel-electrophoresis ...
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bioRxiv - Microbiology 2020Quote: ... Exponential phase cultures were inoculated 1:40 into 7H9 supplemented with kanamycin (Roche, 20ug/ml) and ATc (Sigma 100ng/ml ...
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bioRxiv - Genomics 2020Quote: ... Cells were washed in 2X PIC (Roche mini-tabs, 1 tab in 5 ml = 2X) and stored at -80°C until needed.
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bioRxiv - Microbiology 2019Quote: ... 1 mM DTT) containing Complete™ Protease Inhibitor Cocktail (one tablet per 50 mL) (Roche) and disrupted by sonication (Branson Digital Sonifier ...
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bioRxiv - Microbiology 2021Quote: ... 1 mg/mL DNAse and one complete mini EDTA-free protease inhibitor cocktail tablet (Roche), by passing the sample three times through a pressurised cell disruptor (M110-L ...
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bioRxiv - Bioengineering 2020Quote: ... antigens were retrieved using 1 mg/ml pronase/PBS (11459643001; Roche life science, Indiana, USA) and 10 mg/ml hyaluronidase/PBS (H2126 ...
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bioRxiv - Biochemistry 2022Quote: ... containing 150 mM sodium chloride and 1 mL of 50 x Complete Proteinase inhibitor (Roche 45582400 ...
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bioRxiv - Cell Biology 2022Quote: ... pH 7.4) supplemented with EDTA-free protease inhibitor cocktail (Roche, 1 tablet per 50 ml) and Halt phosphatase inhibitor cocktail (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... SVF pellet was resuspended in 1 mL Red Blood Cell lysis buffer (#41027700, Roche, Germany) and incubated for 5 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... The isolated microvessels were digested in HBSS solution containing 1 μg/ml collagenase/dispase (Roche), 10 U/ml DNase I (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... resuspended in 10 mL digestive media (HBSS + 1% HEPES) with Liberase TM (Roche, Cat: 5401119001) or 1000 U/mL collagenase type IV (Worthington ...
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bioRxiv - Developmental Biology 2021Quote: ... gastruloids were washed once in PBS and digested in 4mg ml−1 dispase I (Roche), 3mg ml−1 collagenase IV (Gibco ...
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bioRxiv - Physiology 2020Quote: Islets were isolated by locally injecting the pancreas with 1 Wünsch unit/ml Liberase (Roche) in HANKS buffer as previously described (22) ...
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bioRxiv - Neuroscience 2021Quote: ... S.p.a) and midazolam (0,5 mg kg −1) (Dormicum®, 5 mg/ml, Roche Pharma, Switzerland) for IM premedication before the start of the procedure ...
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bioRxiv - Biochemistry 2021Quote: ... Primary antibodies used in this study include mouse αGFP (1:1000, 0.4 mg/ml, Roche), rabbit αGFP (1:500 ...
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bioRxiv - Biochemistry 2021Quote: ... Primary antibodies used in this study were mouse αGFP (1:2000, 0.4 mg/ml Roche), rabbit αREX1-repeat (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Livers and lungs were treated with collagenase D (for lung digestion, 1 mg/ml, Roche) or collagenase Type 4 (for liver digestion ...
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bioRxiv - Immunology 2022Quote: ... CNS tissues were cut and digested in DMEM with Collagenase A (1 mg/ml, Roche) and DNase I (0.1 mg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... pH 7.4) containing protease inhibitors (cOmplete mini EDTA-free, Roche, 1 tablet / 10 ml buffer) and homogenized with ten strokes using an overhead mixer rotating at 900 RPM ...