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Citations for Roche :
101 - 150 of 3505 citations for Transcription Factor SOX 10 SOX10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... BMP4 and 6 were prepared by in vitro transcription (DIG RNA labeling kit; Roche Diagnostics). Plasmids to generate cRNA probe for Smad4 was gifted by Prof ...
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bioRxiv - Cancer Biology 2019Quote: Reverse transcription was performed from 1 μg of total RNA using Expand reverse transcriptase (Roche) and oligo dT or random hexamer oligonucleotides as a primer ...
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bioRxiv - Developmental Biology 2020Quote: The Cas9 mRNA were synthesized by in vitro transcription using T7 RNA polymerase (Roche, Mannheim). The target sites of gRNA starting with “GG” or “GA” were designed manually ...
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bioRxiv - Pathology 2021Quote: ... cDNA was then reverse-transcribed using transcription first strand cDNA synthesis kit (Roche Applied Science) with 1 μg total RNA and oligo-dT as primer ...
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bioRxiv - Pathology 2021Quote: ... Digoxigenin-labelled anti-sense riboprobes were made using T3 RNA polymerase transcription kits (Roche Diagnostics). Probe templates for zebrafish col4a3 ...
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bioRxiv - Genetics 2022Quote: ... and reverse transcription was performed on 1µg RNA using Transcriptor First Strand Synthesis kit (Roche). Primer sequences used are listed in Table S1 ...
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bioRxiv - Developmental Biology 2022Quote: Digoxigenin RNA probes were synthesized using the T3/T7 Transcription Kit (Roche; Supp. Table 1). Larvae were collected ...
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bioRxiv - Developmental Biology 2021Quote: RNA probes were prepared by in vitro transcription using the Digoxigenin-RNA labeling mix (Roche). Template plasmids were kindly provided by Drs G ...
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bioRxiv - Genetics 2020Quote: ... The plasmid was linearized and used for in vitro transcription (Roche Dignostics, Indianapolis, IN, USA) to label RNA probes with digoxigenin-UTP ...
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bioRxiv - Neuroscience 2020Quote: All probes were prepared by in vitro transcription using a kit from Roche (Mannheim, Germany) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Reverse primers included a promoter for in vitro transcription using SP6 RNA polymerase (Roche RPOLSP6RO) with incorporation of UTP-digoxigenin (Roche 11093274910) ...
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bioRxiv - Molecular Biology 2022Quote: To determine the repM transcription start site a 2nd Generation 5’/3’ RACE Kit (Roche) was used according to the manufacturer’s instructions with some modifications ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription was performed using the Transcriptor First Strand cDNA Synthesis Kit (Roche, Cat: 04897030001).
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bioRxiv - Cell Biology 2022Quote: ... Reverse transcription (1 μg RNA per sample) was performed using M-MuLV reverse transcriptase (Roche) and random 9-mer primers (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... Riboprobes were generated by in vitro transcription in the presence of Digoxigenin-UTP (Roche Diagnostics). Antisense and sense Charme probes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Reverse transcription into cDNA was performed using the Transcriptor Reverse Transcriptase kit (Roche, Indianapolis, IN) and random hexamer primers (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... and 10% sheep serum] and incubated with a 1:2000 dilution of alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche Cat# 11093274910) in MABT/Block at 4°C overnight ...
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bioRxiv - Plant Biology 2022Quote: ... and the supernatant containing the immunoprecipitated protein was resolved in a 10% SDS-PAGE gel and detected by an anti-HA antibody (Roche, 12158167001).
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bioRxiv - Cancer Biology 2021Quote: ... total RNA was converted into cDNA using the High Capacity cDNA Reverse Transcription Kit (Roche #4368814) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and were immediately subjected to reverse transcription with the Transcriptor First Strand cDNA Synthesis Kit (Roche). Each representative cDNAs of il-6 ...
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bioRxiv - Genetics 2019Quote: ... Probes were synthesized by in-vitro transcription using DIG labeling mix and T7 polymerase (Roche, USA). Hybridization was conducted as previously described (72) ...
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bioRxiv - Neuroscience 2022Quote: ... 1X Transcriptor Reverse Transcription reaction buffer and 10U Transcriptor Reverse Transcriptase (Roche Applied Science, Indianapolis, IN) in 20μL total volume ...
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bioRxiv - Cell Biology 2022Quote: ... Single-stranded Xist riboprobe was generated by in vitro transcription using biotin-16-UTP (Roche, 11388908910) and MAXIscript SP6 kit (Invitrogen ...
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bioRxiv - Physiology 2021Quote: ... and quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed using SYBR green (Roche; #04913914001). Quantification of messenger RNA (mRNA ...
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bioRxiv - Developmental Biology 2020Quote: ... and riboprobes were prepared using the DIG RNA labeling mix and SP6/T7 transcription kit (Roche). Sections were fixed in 4% PFA for 10 minutes ...
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bioRxiv - Developmental Biology 2019Quote: Digoxigenin-labeled cRNA probes were prepared by in vitro transcription (DIG RNA labeling kit; Roche Diagnostics) from the following templates ...
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bioRxiv - Developmental Biology 2021Quote: ... and digoxigenin-labelled probes generated by in vitro transcription using a DIG RNA labelling kit (Roche).
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bioRxiv - Cell Biology 2021Quote: ... and reverse transcribed to cDNA using anchored-oligo(dT)18 primer and Transcriptor Reverse transcription (Roche). DYNLL1 was tested for splicing defects ...
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bioRxiv - Developmental Biology 2020Quote: Digoxigenin-labeled cRNA probes were prepared by in vitro transcription (DIG RNA labeling kit; Roche Diagnostics) from the following templates ...
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bioRxiv - Developmental Biology 2022Quote: ... containing the gene-coding sequences via in vitro transcription using DIG RNA Labeling Kit (Roche, 11175025910). A short bleaching was used to remove the pigments from the fixed zebrafish embryos.
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bioRxiv - Microbiology 2022Quote: ... Digoxigenin (DIG) RNA probes were obtained by in vitro transcription with T7 RNA polymerase (Roche Diagnostics) using PCR-generated DNA fragments as templates ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA synthesis was performed by reverse transcription using the Transcriptor High Fidelity cDNA-Synthesis Kit (Roche) according to the manufacture’s protocol ...
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Planarians employ diverse and dynamic stem cell microenvironments to support whole-body regenerationbioRxiv - Developmental Biology 2023Quote: ... In vitro transcription was carried out at 25 μL scale according to the manufacturer’s instructions (Roche). Reaction products were precipitated by adding 80 μL ice cold ethanol ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was obtained after reverse transcription using a First strand cDNA synthesis RT-PCR kit(Roche) and quantitative PCR was performed using SYBR Green (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... The probes were generated by in vitro transcription in the presence of Digoxigenin-UTP (Roche Diagnostics). Following hybridization ...
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bioRxiv - Developmental Biology 2022Quote: ... Probes were synthesized by in vitro transcription using the DIG and Fluorescein RNA Labeling Mix (Roche). DIG and fluorescein-labeled probes were detected with anti-DIG or anti-Fluorescein POD-conjugated Fab fragments (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA was processed for reverse transcription (RT) using Transcriptor First Strand cDNA Synthesis Kit (Roche). Real-time PCR reactions were carried out using SYBRGreen Master Mix (Roche ...
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bioRxiv - Microbiology 2023Quote: ... 10% SDS) buffer containing 10% protease inhibitor cocktail (Roche) for 30 min on ice ...
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bioRxiv - Biophysics 2023Quote: ... 1% Triton X-100, 10% glycerol, 10 mM NaH2PO4, 10 mM NaP2O7, 100 μM ZnCl2, 10 mM NaF, 1 Roche protease inhibitor tablet), lysed by sonication and the supernatant isolated by centrifugation ...
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bioRxiv - Neuroscience 2020Quote: ... sections were blocked with 10% lamb serum in TBST for 1 hour at RT and incubated with alkaline phosphatase conjugated to DIG antibody (Roche, Basel, Switzerland) in TBST ...
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bioRxiv - Developmental Biology 2022Quote: ... 10% glycerol + 1:10 cOmplete mini protease inhibitor cocktail (Roche)) by incubation on ice followed by sonication (Bioruptor ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin-labeled RNA probes were synthesized by in-vitro transcription using the DIG RNA labeling kit (Roche). In situ analysis was performed as described previously (Cecchetti et al. ...
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bioRxiv - Immunology 2021Quote: ... Quantitative reverse transcription PCR (qRT-PCR) was performed using an SYBR FAST universal qPCR kit (KAPA Biosystems). Predesigned KiCqStart primers for DDX58 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The in vitro transcription using the T7 RNA polymerase and the DIG RNA labelling Mix (both Roche) was performed following the supplier’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... Antisense and sense probes were synthesized by in vitro transcription using a DIG RNA Labeling Kit (Roche). Following labeling ...
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bioRxiv - Biochemistry 2022Quote: Reverse transcription and PCR were performed on 2µg of purified RNA using the Transcriptor Reverse Transcriptase (Roche). RNA was first subjected to DNAse (Roche ...
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bioRxiv - Neuroscience 2019Quote: ... then used as templates for in vitro transcription with RNA T3 polymerase and DIG-labeled NTPs (Roche) to generate DIG-labeled riboprobes ...
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bioRxiv - Molecular Biology 2019Quote: Quantitative reverse transcription PCR was performed with a LightCycler R® 480 II System (Roche Diagnostics, Switzerland). The PCR reaction mixture (10 μl total volume ...
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bioRxiv - Cell Biology 2022Quote: ... Digoxigenin-labeled RNA probes were generated by in vitro transcription using a DIG RNA Labeling Kit (Roche) and purified using Micro Bio-Spin® Chromatography Columns (BioRad) ...
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bioRxiv - Genomics 2022Quote: Real-time quantitative reverse transcription PCR (qRT-PCR) was performed using KAPA SYBR FAST (Kapa Biosystems KK4610) on a 384-well LightCycler 480 Real-Time PCR System (Roche ...