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Citations for Roche :
101 - 150 of 7935 citations for S 6 Methoxy 2 3 dihydro 1H inden 1 amine hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: ... adjust pH to 9.5 adding HCl) for 2 minutes and then tissue was covered with NBT/BCIP stock solution (Roche) diluted in 0.1M TRIS buffer (pH 9.5 ...
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bioRxiv - Physiology 2021Quote: ... Muscles were homogenized in buffer (2% sodium dodecyl sulfate, 50□mM Tris-HCl, pH 6.8) with protease and phosphatase inhibitors (Roche). Protein concentrations were determined by Bicinchoninic acid assay (Sigma-Aldrich ...
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bioRxiv - Physiology 2020Quote: ... were sonicated in lysis buffer (2% SDS, 60 mM Tris-HCl, pH 6.8) with phosphatase and protease inhibitors (Roche). Protein concentration was measured using a BCA assay (Pierce) ...
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bioRxiv - Cancer Biology 2023Quote: ... lysed in Laemmli buffer (62.5 mM Tris-HCl, pH 6.8, 10% glycerol, 2% SDS) supplemented with Complete® protease inhibitors (Roche), and sonicated before protein quantification (DC BioRad Protein Assay Cat No ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections from proximity labeling experiments were lysed and de-crosslinked in 100 mM Tris-HCl buffer (pH = 8.0) with 2% SDS and protease inhibitor (Roche) at 95 °C for 45 min with constant shaking ...
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bioRxiv - Developmental Biology 2023Quote: ... RPE explants were collected in 60 μl of lysis buffer (Tris-HCl 62.5 mM, SDS 2%, pH 6.8) with protease (Roche, #1183617001) and phosphatase inhibitors (ThermoFisher ...
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bioRxiv - Biophysics 2023Quote: Cell lysis was performed by resuspending cell pellets in lysis buffer (50 mM Tris-HCl, 250 mM NaCl, 0.5 mM DTT; 2 µM avidin, protease inhibitors cocktail (Roche) and DNAse (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed in 20 mM Tris-HCl (pH 7.4) with 2% SDS supplemented with a proteinase inhibitor (cOmpleteTM, EDTA-free Protease Inhibitor Cocktail, Roche). Protein concentration of lysates was measured using a bicinchoninic acid (BCA ...
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bioRxiv - Neuroscience 2022Quote: ... Pellets were further extracted by sonication in 3x volumes of SDS lysis buffer (50□mM Tris-HCl, pH 7.4, 2% SDS, 1X Complete Mini protease inhibitor cocktail [Roche]) with centrifugation at 21,000 x g for 30 min at 25°C to obtain the SDS-soluble fraction (Triton-insoluble) ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were thawed and resuspended in 10 µL benzonase buffer (50 mM Tris-HCl pH 8.0, 2 mM MgCl2) with EDTA-free protease inhibitor (Roche, 11873580001 ...
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bioRxiv - Biochemistry 2024Quote: ... and the pellet was resuspended in lysis buffer (25 mM Tris-HCl, pH 8.0, 300 mM NaCl, 1 mM DTT, 1× Benzonase, 1× protease inhibitor cocktail [Roche cOmplete]). The cells were lysed using a cell disruptor at 1.5 kbar ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were counterstained with 4’,6-Diamidine-2’-phenylindole-dihydrochloride (DAPI; Roche).
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bioRxiv - Molecular Biology 2023Quote: ... nuclei were stained using 4’,6-diamidino-2-phenylindole (DAPI, Roche Diagnostics) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: T98G cell pellets were fixed by dropwise addition of cold 70% ethanol and their DNA was stained with 4’,6-diamino-2-phenylindole (1 μg/ml, Roche), 0.1% Triton X-100 in phosphate-buffered saline (PBS) ...
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bioRxiv - Bioengineering 2023Quote: ... The primary antibodies and respective concentrations used in this study are the following: 4’,6-diamidino-2-phenylindole (DAPI) (1:500, Roche), anti-Ki67 (1:100 ...
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Malaria parasite HOPS/CORVET complexes are critical for endocytosis and invasion organelles functionbioRxiv - Cell Biology 2024Quote: ... parasite cultures were incubated for 20 min at 37 °C in RPMI containing 1 µg/mL 4’,6’-diamidine-2’-phenylindole dihydrochloride (DAPI) (Roche) or 2.5 µM Bodipy-TR-C5-ceramide (Invitrogen) ...
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bioRxiv - Developmental Biology 2021Quote: Cell pellets were incubated 20 minutes with RIPA lysis buffer (50 mM Tris-HCl pH7.5, 300 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% SDS, 1X Roche COMPLETE Mini EDTA-free protease inhibitor ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed with RIPA buffer (25 mM Tris HCl [pH 7.6], 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, protease inhibitor cocktail [Roche]). The lysates were subjected to SDS-PAGE under denaturing conditions in 12% polyacrylamide gels using Bio-Rad Mini PROTEAN electrophoresis system (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... the cell pellet was suspended in 1 mL of cold lysis buffer (50 mM TRIS-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.5% NP-40, 1 tablet of Roche cOmplete Protease inhibitor Cocktail ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with glass beads in 250 µl RIPA buffer (25mM Tris/HCl pH 7.6, 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, Roche complete protease inhibitor cocktail ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 mM Tris-HCl [pH 7.5], 1 mM DTT, 0.1% NP-40, and 1× protease inhibitor EDTA-free mixture [Roche]) on ice for 30 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 mM Tris-HCl [pH 7.5], 1 mM DTT, 0.5% NP-40, and 1× protease inhibitor EDTA-free mixture [Roche]) on ice for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... washed 5 times with 500 μL wash buffer (25 mM Tris HCl pH 7.5, 150 mM NaCl, 1.5 mM MgCl2, 1 mM DTT, 1 tablet cOmplete Mini, Roche), and boiled at 95°C for 10 min in resuspension buffer (per reaction ...
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bioRxiv - Cell Biology 2024Quote: ... blocked for 1h in 0.5% Roche blocking solution (Roche, #11096176001), then incubated overnight at 4°C with primary antibody diluted in blocking reagent ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were lysed in 50 μl of Lysis Buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40, 1x Roche Complete protease inhibitors cocktail) by pipetting up and down ...
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bioRxiv - Immunology 2021Quote: The measurement of anti SARS-CoV-2 neutralizing Abs was performed by electrochemiluminescence sandwich immunoassay (ECLIA) through Roche Elecsys Anti-SARS-CoV-2 S (Roche diagnostics, Switzerland). The neutralizing Ab were measured on a Cobas 601 modular analyzer (Roche diagnostics ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM DTT and 0.2 M HCl) freshly complemented with 1× Proteinase Inhibitor Cocktail (Roche) and 10 mM N-ethylmaleimide (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: ... cell pellets were resuspended in HR buffer (50 mM Tris-HCl, 5 mM MgCl2, 250 mM sucrose, 2 mM TCEP and protease inhibitor t (Roche), pH 7.4) ...
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bioRxiv - Microbiology 2022Quote: ... and lysed by incubation for 30 min on ice in non-denaturing lysis buffer (300 mM NaCl, 100 mM Tris-HCl, pH 7.4, 2% Triton X-100, and 1x EDTA-free protease inhibitor cocktail [Roche Complete]). For immunoblot assays ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed in lysis buffer (2% SDS in 50 uM Tris-HCL pH.8, supplemented with EDTA-free protease inhibitor, Roche) at cell concentration of 20×106/mL and incubated at 95 °C for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... then with 1 ml of Western blot buffer (2% SDS, 5% Glycerol, 50 mM Tris-HCl, 0.2 M EDTA + cOmplete protease inhibitor cocktail tablet (Roche, 11697498001) + PhosSTOP (Roche ...
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bioRxiv - Biophysics 2021Quote: ... Tris-HCl (10812846001) was purchased from Roche. DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine ...
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bioRxiv - Synthetic Biology 2022Quote: ... Tris-HCl (10812846001) was purchased from Roche. DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine ...
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bioRxiv - Molecular Biology 2024Quote: ... The resin was washed five times with 10 CV of Nickel Buffer (25 mM HEPES pH 7.6, 0.3 M NaCl, 10 mM imidazole, 2 mM DTT, 0.01% IGEPAL CA-630, 10% glycerol, 1× Roche cOmplete protease inhibitors). Protein was eluted with Nickel Buffer supplemented with 300 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... resuspended with 1 mL lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl PH7.0, freshly added 1 mM PMSF, complete protease inhibitor [Roche]) for 10 min at 4°C and subjected to sonication to fragment the genomic DNA in size range of 300-700 base pair ...
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bioRxiv - Microbiology 2021Quote: ... 109 CFUs of bacteria were resuspended in 150 µL of buffer TE (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) with 5 mg mL-1 of lysozyme (Roche) for 5 minutes at room temperature ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... cells were lysed with 100 µl Lysis buffer (10 mM Tris-HCl, pH 7.5, 300 mM NaCl, 1 mM EDTA, 1% Triton X-100, Protease Inhibitor Cocktail Roche). The cell pellet was resuspended by pipetting and incubated on ice for 30 min ...
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bioRxiv - Developmental Biology 2020Quote: ... Whole cells extract was isolated using RIPA buffer (NaCl 150mM – Tris HCL pH7,35 50mM – DOC 1% – N-P40 1% – H2O) supplemented with protease inhibitor (04 693 116 001, Roche). Isolated protein concentration was determined using Bradford assay (500-0006 ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were prepared by resuspending cell pellets in 2 mL NP-40 buffer (50 mM Tris-HCl pH 7.8, 150 mM NaCl, 1% NP-40, 1 mM PMSF, 1x PhosSTOP [Roche]), sonication on ice (5s on/10s off for 2 mins at 40% amplitude) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated in staining solution (1 M Tris-HCl pH 9.5, 5 M NaCl, 1 M MgCl2, NBT-BCIP, H2O) (Roche) in the dark for 10 min at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... the sections were washed in a serial SSC buffer and formamide solution and then preincubated in buffer 1 (100 mM Tris-HCl, pH 7.5, 150 mM NaCl) with a 1% blocking reagent (Roche) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... the cell pellet was resuspended in 10 mL of column buffer (20 mM Tris-HCl pH 7.5, 200 mM NaCl, 1 mM EDTA, protease inhibitors [1 Roche Mini EDTA-free tablet] ...
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bioRxiv - Microbiology 2023Quote: 293T cells transfected with pCAG AcGFP-FOS or pCAG AcGFP-JEVcore-FOS were incubated for 48 h and lysed in lysis buffer (20 mM Tris-HCl [pH = 7.4], 135 mM NaCl, 1% Triton X-100, 1% glycerol, and protease inhibitor cocktail tablets [Roche]). The cell lysates were incubated with Strep-Tactin Sepharose (IBM GmbH ...
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bioRxiv - Plant Biology 2024Quote: ... Root tissues were ground with liquid nitrogen in a mortar before homogenization with the extraction solution (20 mM Tris-HCl, pH 7.0, 1 mM EDTA, 1 mM DTT and a cocktail of protease inhibitors, Roche). The suspension was ultracentrifuged at 105,000 g for 20 min ...
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bioRxiv - Neuroscience 2022Quote: ... previously equilibrated in Wash buffer 1 (50 mM Tris-HCl pH 7.5, 150 nM NaCl, 1% NP-40, 1x protease inhibitors (Roche)) for 3 times ...
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bioRxiv - Genomics 2022Quote: ... before being re-pelleted by centrifuging for 3 minutes at 4°C at 500g and resuspended in 180µl TMS buffer (10mM Tris-HCl pH 8, 1mM MgCl2, 1% SDS, 1× Complete Protease Inhibitor Cocktail (PIC, Roche)) with 0.4µl benzonase (E1014 ...
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bioRxiv - Immunology 2022Quote: ... the cells were lysed with ChIP-Lysis buffer (10 mM EDTA, 1% [w/v] SDS, 50mM Tris-HCl, pH 7.5, 1 tablet Protease Inhibitor Cocktail [#11836170001; Roche, Mannheim ...
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bioRxiv - Immunology 2024Quote: ... Cells were lysed in RIPA buffer (50 mM Tris HCL, 150 mM NaCl, 1% SDS, 0.5% Na-deoxycholate, 1× Complete protease inhibitor (Roche)) for 30 min on ice ...
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bioRxiv - Cell Biology 2024Quote: Cell pellet was collected and re-suspended in lysis buffer (25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol, Roche complete protease inhibitor ...
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bioRxiv - Biochemistry 2024Quote: ... 1% PMSF (Roche Life Sciences, 329-98-6), 25 U benzonase nuclease (EMD ...