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Citations for Roche :
101 - 150 of 1132 citations for Recombinant Human CD96 Protein T7 His TEV tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... T7 RNA polymerase (Roche, cat. no. 10881767001), T3 RNA polymerase (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... and T7 or T3 RNA polymerase (Roche) was used ...
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bioRxiv - Developmental Biology 2019Quote: ... using T7 polymerase (Roche Digoxigenin labelling Kit).
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bioRxiv - Physiology 2024Quote: ... SP6 or T7 RNA polymerase (Roche Diagnostics), and the following cDNA fragments ...
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bioRxiv - Molecular Biology 2019Quote: ... and mouse monoclonal antibody against the human P16 protein (Ventana Roche-E6H4, USA) was used in the immunostaining assay.
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bioRxiv - Plant Biology 2021Quote: ... Immunoblot with the anti-His-antibody conjugated to peroxidase (BMG-His-1 monoclonal antibody; Roche, Basel, Switzerland) was used to confirm the presence of enzymes.
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bioRxiv - Biochemistry 2022Quote: (His)6-GST-SNX15 MIT was bound to cOmplete His-Tag purification beads (5 mL, Roche, Germany, 2h) and washed with 2 L wash buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... RNA probes were generated using PCR with T7 promoter sequence linkers and subsequently transcribed [DIG or FITC labeled] using T7 polymerase (Roche). Primer sequences are all found in Table 3 ...
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bioRxiv - Bioengineering 2020Quote: The cell-free reaction mixture for the KGK10 strain is identical to that used for the T7 SHuffle® with the addition of 100 μg/mL DsbC (GeneFrontier) and 3.33 Units/uL T7 RNAP (Roche). Previously prepared KGK10 extract fermented and processed by the Swartz lab was tested alongside the in-house KGK10 extract prepared by shake flasks.
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bioRxiv - Developmental Biology 2019Quote: ... DIG and FITC labeled RNA probes were generated using PCR incorporating T7 promoters in the primers and ultimately transcribed with T7 polymerase (Roche). Forward and Reverse primer sequences are listed in Supplemental Table 1 ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products with flanking T7 promoters were used as template for the synthesis of anti-sense riboprobes using a T7 RNA polymerase (Roche) (Mercurio et al ...
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bioRxiv - Neuroscience 2024Quote: ... and T7 promoter sequence was added on all the reverse primers for generating antisense mRNA probes using T7 RNA polymerase (Roche). For immunohistochemistry (IHC ...
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bioRxiv - Plant Biology 2024Quote: ... and 1/5000 anti-His (11667475001, Roche) for GFP and His-Tag detection ...
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bioRxiv - Molecular Biology 2022Quote: Recombinant HBsAg (Roche Diagnostics International) was used as a marker for aerosol formation ...
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bioRxiv - Synthetic Biology 2022Quote: ... with recombinant DNase I (Roche) treatment ...
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bioRxiv - Synthetic Biology 2022Quote: ... and recombinant proteinase K (Roche). Samples were run on a 1% certified megabase agarose (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... incorporating digoxigenin (DIG) (SP6/T7; Roche, Basel, Switzerland), as previously published (Cornish et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... and the T3 or the T7 polymerase (Roche) depending on the insert orientation ...
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bioRxiv - Developmental Biology 2019Quote: ... The DIG RNA Labeling kit (SP6/T7; Roche) was used for in vitro transcription using PCR products cloned into the pCR4-TOPO vector (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... using T7 and SP6 polymerases (Roche 10881767001 & 10810274001). Whole mount in situ hybridization was performed as previously described (Thisse and Thisse ...
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bioRxiv - Developmental Biology 2021Quote: ... sgRNA was synthesized using T7 RNA polymerase (Roche). AmpliCap SP6 High Yield Message (CellScript ...
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bioRxiv - Developmental Biology 2019Quote: ... Riboprobes were synthesized with T7 RNA polymerase (Roche) and DIG-labeling mix (Roche) ...
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bioRxiv - Cell Biology 2021Quote: ... and either T7 or T3 RNA polymerase (Roche). Transcripts were carbonate-treated to reduce their size.
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bioRxiv - Genetics 2020Quote: ... and transcribed with T7 RNA Polymerase (10881767001, Roche). We used a PCR-based DNA template to make the hmx3b ISH probe ...
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bioRxiv - Developmental Biology 2022Quote: ... DIG-RNA labelling kit (Sp6/T7) (Roche Diagnostics) was used for DIG or Fluorescein RNA labelling following instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... T7 polymerase and biotin labeling mix (Roche #11685597910) were used according to the package directions ...
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bioRxiv - Biochemistry 2021Quote: ... the recombinant protein was expressed in HEK293T cells and released by 100 μM digitonin in PBS with protease inhibitor cocktail (Roche). The cell lysate prepared with the same method from HEK293T was used as the negative control.
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bioRxiv - Synthetic Biology 2023Quote: ... and 8G8-Fab were expressed with His-tag and purified using the cOmplete His-Tag purification resin (Roche, USA) following standard protocols ...
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bioRxiv - Plant Biology 2022Quote: ... mCIT tagged proteins were revealed by using respectively GFP monoclonal antibody (anti-GFP mouse monoclonal, Roche; at 1/1000 in 5 % milk over-night) as primary antibodies and anti-mousse IgG-HRP conjugated secondaries antibodies (Mouse IgG ...
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: ... and GFP- tagged pCREB3L3 using X-tremeGENE 9 (Roche). Cells were grown on coverslips ...
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bioRxiv - Biophysics 2020Quote: ... and amplified using KAPA Hi Fi polymerase (Roche) in 20 cycles of emulsion PCR35 (ePCR ...
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bioRxiv - Biochemistry 2022Quote: ... purified by cOmplete His-Tag purification columns (Roche), and concentrated to 1 mg/mL using Amicon Ultra 10,000 NMWL (Merck) ...
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bioRxiv - Microbiology 2020Quote: ... and cOmplete™ His-tag Purification Resin (Roche). Purity and glycosylation of recombinant proteins were examined by PNGase F (N-Zyme Scientifics ...
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bioRxiv - Developmental Biology 2022Quote: ... the cOmplete his-tag purification matrix from Roche is working equally well ...
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... Complete His-Tag Purification Resin (Roche, Basel, Switzerland), or Amylose Resin (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... using T7/Sp6 RNA polymerase (Roche Diagnostics, Basel, Switzerland). Anti-DIG alkaline phosphatase antibody (Roche Diagnostics ...
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bioRxiv - Developmental Biology 2020Quote: ... driven by T7 RNA polymerase with DIG incorporation (Roche). Chromogenic and fluorescent RNA in situ hybridization were carried out as previously described50,51 ...
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bioRxiv - Neuroscience 2021Quote: ... Standard in vitro transcription methods using T7 polymerase (Roche) and digoxigenin-UTP RNA labeling mix (Roche ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The riboprobes were synthesized using T7 RNA polymerase (Roche) and DIG-labelling mix (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA probe synthesis was performed using T7 polymerase (Roche) with either DIG RNA labelling mix (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... and ∼40 U of either T7 (Roche/Sigma, UK) or SP6 (Roche/Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... together with T7 RNA polymerase (Roche, Cat. No. 10881775001), SP6 RNA polymerase (Roche ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant IFN-α2a (Roferon L03AB04, Roche) and IFN-λ1 (Peprotech 300-02L ...
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bioRxiv - Neuroscience 2022Quote: ... tagged cDNA synthesized using the KAPA mRNA HyperPrep kit (Roche). The prepared libraries were sequenced on an Illumina NextSeq 500 using High Output Flowcell Cartridge from the NextSeq 500/550 Output v2 kit (75 cycles ...
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bioRxiv - Molecular Biology 2023Quote: ... Digoxigenin-containing RNA probes were generated by in vitro transcription with T7 polymerase using the DIG RNA Labelling kit (SP6/T7) from Roche (cat. No 11175025910). The oligonucleotide AJ529 (T7 promoter ...
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bioRxiv - Cancer Biology 2021Quote: ... 12.5 μL of Kappa Hi Fi Hotstart ReadyMix (Roche) and each 0.75 μL (0.3 μM ...
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bioRxiv - Biochemistry 2020Quote: ... and incubated with cOmplete His-Tag purification resin (Roche) for 16 hours on a tube roller (Starlab ...
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bioRxiv - Cancer Biology 2021Quote: ... 12.5 μL of Kappa Hi Fi Hotstart ReadyMix (Roche) and each 0.75 μL (0.3 μM ...
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bioRxiv - Plant Biology 2020Quote: ... 12 µl of complete His-Tag Purification Resin (Roche) was added and incubated for 15 min at 25°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we used Kapa Hi-Fidelity Library Amplification Kits (Roche) in 20 µL reactions with 4µL Nextera Unique Dual Indexes Set A (Illumina) ...