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Citations for Roche :
101 - 150 of 6666 citations for Human Putative Alpha 1 Antitrypsin Related Protein SERPINA2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were proteolyzed with sequencing grade trypsin (Roche) using 0.01% Protease-MAX surfactant (Promega).
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bioRxiv - Plant Biology 2022Quote: ... Protein detection was carried out using peroxidase-coupled anti-HA-Peroxidase antibodies (Roche, dilution 1/4000), peroxidase-coupled anti-GFP antibodies (Milteneyi ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: ... Cell lysates (1 mg) were pre-cleared with Protein-G–agarose beads (Roche, catalog number 11719416001) for 1 h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins with HA-tag(s) were detected using rat mAb-anti-HA 3F10 (Roche, 1:1000) as primary antibody and IRDye® 800CW Goat anti-Rat IgG (Licor ...
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bioRxiv - Immunology 2020Quote: ... cells were cotransfected with both the reporter gene and one of the different putative transcription factors using X-tremeGENE 9 (Roche) following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2019Quote: Total proteins from mammalian cells were extracted from cells by resuspension and homogenization in RIPA buffer containing protein inhibitors (Complete, Roche Diagnostics) and phosphatase inhibitors (Phospho-STOP ...
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bioRxiv - Cell Biology 2019Quote: ... were incubated with specific antibodies overnight and then with 60 μl of slurry of Protein A- or Protein G-agarose beads (Roche, Mannheim, Germany) for 3 h ...
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bioRxiv - Cell Biology 2019Quote: ... were incubated with specific antibodies overnight and then with 60 μL of a slurry of Protein A- or Protein G-agarose beads (Roche, Mannheim, Germany) for 3 h ...
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bioRxiv - Microbiology 2021Quote: ... transferred to PVDF membranes and protein expression was detected using anti HA (3F10, Roche 11867423001, 1:5000) followed by horseradish peroxidase (HRP)-conjugated anti-rat antibodies (Sigma ...
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bioRxiv - Plant Biology 2022Quote: ... For every biological replicate 500 μg protein was digested with sequencing grade trypsin (1:100 trypsin:protein; Roche) overnight at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: Proteins were extracted using 1xPBS pH 7.4 with Protease Inhibitor Cocktail (1 tablet/10 mL) (Roche Diagnostics) and sonicated during 10 seconds at 10% power ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were subjected to Western blot analysis with mouse monoclonal anti-GFP antibody (1:2,000 dilution, Roche), rabbit polyclonal anti-Sec2 antibody (1:2000 dilution ...
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bioRxiv - Plant Biology 2023Quote: ... Protein blots were probed with anti-HA antibodies conjugated to horseradish peroxidase (Roche, Switzerland, 12013819001, 1:4000). Immunoblots were visualised with Pierce ECL Plus Western Blotting Substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... proteins were detected using ECL lumi-light plus (Roche) and images acquired using camera system (Chemitouch Biorad).
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred to nitrocellulose or PVDF membranes (Roche) in tris-glycine buffer at 110 V for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... and protein extracted with ELB buffer containing PhosSTOP (Roche) and 50 μM sodium fluoride ...
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bioRxiv - Cell Biology 2019Quote: ... protein inhibitor and phosphatase inhibitor (Complete-EDTA free, Roche/Sigma prod ...
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bioRxiv - Cancer Biology 2019Quote: BSA Protein Assay Kit (A8020-5, Roche, Basel, Switzerland) was applied to measure the protein concentration after lysing BC cells with RIPA buffer ...
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bioRxiv - Immunology 2019Quote: Proteins were digested on beads using trypsin (Roche, Germany) in 6 M Urea ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were transferred onto polyvinylidene fluoride (PVDF) membrane (Roche) for 80V for 1.5 hours ...
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bioRxiv - Biochemistry 2019Quote: ... and then incubated with protein A–agarose beads (Roche) for 4 h ...
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bioRxiv - Genomics 2019Quote: ... protease inhibitors) and precleared with protein A beads (Roche) at 4 °C for 1 hr ...
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitations were performed using Protein A agarose beads (Roche). DivJ was immunoprecipitated using an anti-DivJ antiserum (15) ...
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bioRxiv - Cancer Biology 2021Quote: ... at 4 °C overnight and protein G-Agarose (Roche) at 4 °C for 3 h ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were semi-dry blotted onto PVDF membranes (Roche), which were subsequently blocked in 5 % milk in PBST ...
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bioRxiv - Cell Biology 2021Quote: ... followed by incubation with Protein G beads (Roche, 1124323301) for 2 hours at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred onto polyvinylidene fluoride (PVDF) membrane (Roche) for 30V for 16 hours ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were reduced using 10 mM dithiothreitol (Roche) and alkylated with 50 mM iodoacetamide ...
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bioRxiv - Neuroscience 2021Quote: ... Then homogeneous protein G-agarose suspension was added (Roche) to the suspension ...
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bioRxiv - Pathology 2019Quote: ... Proteins were detected using polyclonal antibodies against GFP (Roche), Histone H3 (GeneTex ...
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bioRxiv - Plant Biology 2021Quote: ... coupled to 50µl bed volume Protein A agarose (Roche) per sample ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM DTT and 1x protein inhibitor cocktail (Roche), and incubated at 25ºC for 1 h on a rotating wheel ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation with protein G-agarose beads (Roche) for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were transferred onto PVDF membranes (Roche, Basel, Switzerland), and Western blot detection was carried out as previously described [32,33] ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were reduced using 10 mM dithiothreitol (Roche) and alkylated with 50 mM iodoacetamide ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were reduced using 10 mM dithiothreitol (Roche) and alkylated with 50 mM iodoacetamide ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated with protein A agarose beads (Roche, #11134515001) at 4 °C overnight with shaking ...
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bioRxiv - Cancer Biology 2023Quote: ... Following electrophoretic transfer of proteins onto PVDF membranes (Roche), nonspecific binding was blocked by incubation with 5% BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... 400 µg of protein were reduced using DTT (Roche, Cat#10708984001 ...
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bioRxiv - Physiology 2020Quote: ... The expression of all genes was related to an internal housekeeping gene assay for Actb (Roche, Welwyn, UK) as described previously (O’Hara et al. ...
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bioRxiv - Neuroscience 2021Quote: ... mice and lysed on ice for 30 min in protein lysis buffer (1 mM Na3VO4, 1 mM NaF, complete protease inhibitor cocktail (Roche, USA), 1% Triton X-100 in PBS) ...
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bioRxiv - Neuroscience 2023Quote: ... pH 7.4 with 0.1% Triton X-100) and then incubated with 350 µg protein in 1:1 8M urea with Complete protease inhibitor (Roche 11245200) sample lysis:Buffer A/0.5% Triton X-100 (150 mM NaCl ...
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bioRxiv - Biophysics 2024Quote: ... Cultures were grown until an A600 of 0.6-0.8 was reached and protein over-expression was induced by incubating with 1 mM isopropyl-β-d-1-thiogalactopyranoside (IPTG, Roche) for 16 hours at 18°C.
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bioRxiv - Cancer Biology 2024Quote: ... Total protein were extracted from cells and tissues using protein lysis buffer supplemented with protease and phosphatase inhibitors (Roche Applied Science, Penzberg, Germany). Protein concentrations were assayed by the Pierce™ BCA Protein Assay Kit (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Putative hygromycin and neomycin-resistant candidates transformants were screened on TB3 media supplemented with 250 μg/mL hygromycin B (Roche Applied Science) and 200 μg/mL G418 (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... Small fragments of DNA covering the putative cleavage sites were amplified by PCR with KAPA Hifi HotStart Ready Mix (KAPA Biosystems, KK2602) from the genomic DNA using primers p13 and p14 ...
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bioRxiv - Plant Biology 2023Quote: ... The complete cDNA encoding the complete starch branching enzyme mature isoforms (devoid of their putative transit peptides) were amplified from a Chlamydomonas cDNA bank using the proof-reading Kapa Hifi Hot start polymerase (Roche Diagnostics, Austria) and cloned into the pENT-D-Topo plasmid (Thermofisher ...
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bioRxiv - Developmental Biology 2019Quote: ... Supernatant containing cytosol protein was mixed with 1% SDS solution supplemented with proteinase inhibitor (PI) cocktail (Roche, 5892970001), and then was used for western blotting ...
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bioRxiv - Microbiology 2019Quote: ... The total protein pellets were resuspended in 1 mL NP-40 with protease inhibitor cocktail (Roche Diagnostics GmbH). Samples were pre-cleared using Protein A/G plus agarose beads (Pierce ...
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bioRxiv - Biochemistry 2024Quote: ... HA- and GFP-tagged proteins were detected using horseradish peroxidase-conjugated monoclonal anti-HA (Roche, 3F10, 1:5,000) and anti-GFP (Roche ...