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Citations for Roche :
351 - 400 of 6321 citations for Human Putative Alpha 1 Antitrypsin Related Protein SERPINA2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... total protein extracts were prepared in lysis buffer supplemented with protease and phosphatase inhibitors (Roche). Proteins were quantified by bicinchoninic acid (BCA ...
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bioRxiv - Neuroscience 2024Quote: ... samples were captured with 50 µL of 50% (w/v) protein-A agarose beads (Roche), and the reaction was kept at 4°C for 3 hr on a rotary shaker ...
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bioRxiv - Developmental Biology 2024Quote: ... The remaining lysate was rotated overnight with 50 µl of Protein A-agarose beads (Roche) and 2 µg of mouse anti-HA (monoclonal ...
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bioRxiv - Cell Biology 2020Quote: Protein was harvested in urea ammonium bicarbonate lysis buffer with complete EDTA free protease inhibitor (Roche) and phosphatase inhibitor (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... followed by incubation with 60 µg of Protein A- or G-agarose bead slurry (Roche, Germany) for 4 hours at 4 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... proteins were digested by addition of 40 µl proteinase K (Roche, dissolved to 10 µg/µl) and incubation for 2 hours at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Protein was extracted from cell fractions using RIPA buffer containing protease inhibitor complete EDTA free (Roche) and phosphatase inhibitor cocktail (Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... The FLUC-HA and NLUC-HA proteins were detected with anti HA-HRP antibody (Roche 3F10) (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and hybridized to a mouse monoclonal antibody against the P16 protein (Ventana Roche Diagnostics, E6H4, Switzerland) overnight at 4°C ...
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bioRxiv - Physiology 2021Quote: ... triglycerides and total protein were quantified using the Cobas c111 analyzer (Roche diagnostics GmbH, Mannheim Germany) as previously described by Segev-Hadar et al.38 ...
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bioRxiv - Biochemistry 2020Quote: ... The protein eluted after immobilized metal affinity chromatography (IMAC) using complete His-tag purification resins (Roche) was concentrated using 10KDa cut-off amicon filters (Merck ...
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bioRxiv - Bioengineering 2021Quote: ... pastoris culture medium containing the recombinant protein was purified by affinity chromatography Ni-NTA agarose (Roche).
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bioRxiv - Molecular Biology 2022Quote: ... cell culture supernatants were harvested and proteins were purified from the supernatants using tandem Ni2+ (Roche) and Strep-Tactin (IBA ...
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bioRxiv - Molecular Biology 2019Quote: Total protein lysates were prepared using RIPA lysis buffer containing protease inhibitors (Complete Ultra Tablets, Roche). Equal amounts of total lysates were separated by SDS-PAGE (4–15% ...
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bioRxiv - Cancer Biology 2020Quote: Whole-cell protein lysates were harvested in lysis buffer (RIPA buffer supplemented with protease inhibitor (Roche), sodium vanadate and sodium molybdate ...
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bioRxiv - Biochemistry 2020Quote: ... coli cell pellets were resuspended in protein buffer containing cOmplete protease inhibitor cocktail (Roche, Basel, Switzerland), lysed by sonication and centrifuged for 45 min at 38,000 g to remove cell debris ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were subsequently detected with HRP-conjugated anti-HA antibody (rat monoclonal, Roche, catalog number 12013819001) or mouse monoclonal anti-GFP antibody (Novus Biologicals ...
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bioRxiv - Molecular Biology 2019Quote: ... and then Cas9 proteins were digested with 0.5 µl of proteinase K (∼20 mg/ml, Roche) at 37°C for 10 min ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The protein extraction was performed by adding 10 M urea containing complete protease inhibitor cocktail (Roche) and Halt™ Phosphatase Inhibitor (Thermo ...
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bioRxiv - Microbiology 2020Quote: ... the supernatants were incubated in presence of 20 µl of protein G agarose beads (Roche, 11243233001) for 3 h at 4°C on a rotor to reduce unspecific binding ...
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bioRxiv - Biophysics 2020Quote: ... The expressed protein is first purified by affinity chromatography using Ni-NTA coated agarose beads (Roche). His6-tagged proteins are eluted using 250 mM imidazole ...
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bioRxiv - Plant Biology 2022Quote: ... Tagged SAC9 fusion proteins were revealed by using GFP monoclonal antibody (anti-GFP mouse monoclonal, Roche) and detected by chemiluminescence using ECL revelation as for T-PLATE and SH3P2 fusion proteins.
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bioRxiv - Molecular Biology 2022Quote: ... The clarified lysate was incubated with 2 mL of pre-equilibrated Protein C affinity resin (Roche) for 3 h at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... The extract was then precleared by incubation with 50 ul of Protein A-agarose beads (Roche) at 4°C for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... and homogenized in a T-PER tissue protein extraction reagent supplemented with protease (Roche Applied Science) and phosphatase inhibitors (Millipore) ...
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bioRxiv - Plant Biology 2023Quote: ... The extract was then precleared by incubation with 50 ul of Protein A-agarose beads (Roche) at 4°C for 30 min ...
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bioRxiv - Immunology 2023Quote: ... and equal amounts of protein from lysate were incubated with 5 μg anti-FLAG antibody (Roche) overnight ...
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bioRxiv - Biochemistry 2023Quote: ... protein eluates from every purification step were immediately supplemented with protease inhibitors (EDTA-free cOmplete, Roche) (together with DTT at 10 mM for cysteine-containing protein constructs) ...
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bioRxiv - Cancer Biology 2023Quote: Protein lysates were extracted with ice-cold RIPA buffer supplemented with protease and phosphatase inhibitors (Roche). Thirty μg of protein lysates were separated using SDS–polyacrylamide gel electrophoresis and transferred to a PVDF membrane (Immobilion-P ...
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bioRxiv - Cancer Biology 2023Quote: Whole-cell protein lysates were harvested in lysis buffer (RIPA buffer supplemented with protease inhibitor (Roche), sodium vanadate and sodium molybdate ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was incubated with 2 ml of pre-equilibrated Protein C affinity resin (Roche) for 2h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... HBE were processed for protein lysates and stored at -20°C (Complete Lysis-M Roche diagnostics). NET dosage was an average of extracellular dsDNA concentration (QuantiFluor ONE dsDNA System ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with 30 μl of pre-washed Protein A agarose bead slurry (#11134515001, Roche) for an additional hour (4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Protein lysates were prepared from zebrafish larvae (6 dpf) in RIPA buffer containing protease inhibitors (Roche), using a manual dounce homogenizer ...
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bioRxiv - Physiology 2024Quote: Protein cardiac extracts were obtained by tissue homogenization with ceramic beads (MagNa Lyser Green Beads, Roche) in lysis buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2024Quote: ... and 250 mg or 500mg protein was digested with 20 µg/ml proteinase K (PK) (Roche) for PrPSc blots for 1 hour at 37C ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% Penicillin/Streptomycin and 20 IU human IL-2 (Roche Diagnostics, Mannheim, Germany) for 5d ...
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bioRxiv - Microbiology 2020Quote: ... Protein lysates were obtained by lysing cells in 0.1% SDS and protease inhibitor cocktail tablets (Roche #04693124001). Cell lysates were separated by SDS-PAGE (Bio-Rad 4-20% Mini-PROTEAN TGX Precast Gel ...
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bioRxiv - Molecular Biology 2019Quote: ... proteins were digested by addition of 40 µl proteinase K (Roche #03115852001, dissolved to 10 µg/µl) and incubation for 2 hours at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... proteins were digested by addition of 40 µl proteinase K (Roche #03115852001, dissolved to 10 µg/µl) and incubation for 2 hours at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 µl anti-CENP-ACnp1 (sheep in-house antiserum) and 25 µl Protein-G-Agarose beads (Roche) or 2 µl of anti-GFP (A11122 ...
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bioRxiv - Cell Biology 2019Quote: ... samples were further incubated for 2 hrs in 40 µl pre-cleared protein-G agarose beads (Roche). Beads with immunocomplexes were centrifuged at 3,000 × g and washed four times in lysis buffer with intermittent incubations ...
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bioRxiv - Genetics 2019Quote: Total protein was extracted from transfected HEK293 cells lysed in RIPA buffer (Pierce) containing protease inhibitors (Roche) 24 hours post transfection and size separated using SDS-PAGE ...
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bioRxiv - Biochemistry 2019Quote: ... and then scraped in 10 ml PBS + 1X Protein Inhibitor Cocktail buffer (catalog no. COUL-RO, Roche) and centrifuged for 5 min at 1,000 x g at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... The eluted proteins were subjected to 10% SDS-PAGE gels for immunoblot analyses using anti-GFP (Roche) and anti-Myc (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse/rabbit IgG overnight at 4°C and further with protein G-coupled agarose beads (ROCHE) for 1-2 h ...
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bioRxiv - Cell Biology 2020Quote: Cells were lysed and proteins were purified by Complete™ Lysis-M EDTA-free kit (Roche, 04719964001). Protein samples were run on Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: Total proteins were extracted from cells using RIPA lysis buffer with a protease inhibitor cocktail (Roche, Germany). The proteins were quantified using Thermo Scientific Pierce BCA Protein Assay ...
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bioRxiv - Microbiology 2020Quote: Protein lysates of HEK-293T cells were extracted with RIPA buffer (PIERCE) and protease inhibitor cocktail (Roche). Loading buffer (Life Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were western blotted onto PVDF membranes and probed with the following antibodies: anti-HA (11867423001, Roche), anti-Flag (F3165 ...