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Citations for Roche :
101 - 150 of 6405 citations for AS 1517499 CAS 919486 40 1 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 1 mM PMSF, 0.05% NP-40, 10% glycerol, 1×Roche protease inhibitor cocktail) and were lysed by beating with 0.5-mm-diameter glass beads using a FastPrep instrument at a speed of 6.5 m/s for three cycles of 20 seconds each ...
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bioRxiv - Molecular Biology 2020Quote: Cells were lysed in lysis buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 1% Nonidet P-40, 1 mM sodium orthovanadate and 1× complete protease inhibitor cocktail from Roche) at 4°C for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining extract was centrifuged at 2,000 rpm for 5 minutes and re-suspended in 1 x PBS Lysis buffer (1 x PBS, 0.01% NP-40, 5% Glycerol, 150mM NaCl, 1 x Roche cOmplete), sonicated and stored at -20°C for downstream applications.
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bioRxiv - Cell Biology 2023Quote: Decapsulated testis extract was re-suspended into 1 x PBS Lysis buffer (1 x PBS, 0.01% NP-40, 5% Glycerol, 150mM NaCl, 1 x Roche cOmplete) and sonicated for 20 s at 22% amplitude in cycles of 0.4 s on and 0.2 s off ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants were diluted 1:4 with ice-cold dilution buffer (1× phos-stop [Roche], 0.5% NP-40, 1 mM DTT) and centrifuged again at 15,000 rpm for 15 minutes at 4°C to precipitate actomyosin components ...
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bioRxiv - Genomics 2024Quote: ... The chromatin-bound beads were washed with 4 × 1 ml of wash buffer (50 mM Hepes-KOH pH 7.6, 250 mM LiCl, 1 mM EDTA, 1% NP-40, 0.7% Na-Deoxycholate, 1 × Roche cOmplete Mini) for 10 minutes on a rotating wheel ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was replaced with RIPA buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl, 1 mM MgCl2, 1% NP-40, 0.5% sodium deoxycholate, 1x complete protease inhibitor [Roche]) including 1% SDS ...
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bioRxiv - Neuroscience 2021Quote: ... 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA and 1X protease inhibitor cocktail; Roche). Anti-FLAG M2 Magnetic Beads (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed with RIPA buffer (25 mM Tris HCl [pH 7.6], 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, protease inhibitor cocktail [Roche]). The lysates were subjected to SDS-PAGE under denaturing conditions in 12% polyacrylamide gels using Bio-Rad Mini PROTEAN electrophoresis system (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl, 1% NP-40, 0.25% sodium deoxycholate, 0.1% SDS, 2 mM EDTA, 1 mM PMSF, Roche protease and phosphate inhibitor cocktails ...
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bioRxiv - Physiology 2020Quote: ... 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, protease inhibitors [Roche], phosphatase inhibitors [Roche]). Lysates were incubated for 15 minutes on ice and centrifuged twice at 15,000 x g for 20 minutes at 4°C to remove insoluble material ...
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bioRxiv - Microbiology 2021Quote: ... the cell pellet was suspended in 1 mL of cold lysis buffer (50 mM TRIS-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.5% NP-40, 1 tablet of Roche cOmplete Protease inhibitor Cocktail ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM EDTA, 0.1% NP-40, 1 mM DTT, 1 mM PMSF, 1.3 mM benzamidine, protease inhibitor tablets [Roche]) and eluted with maltose (20 mM Tris pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.5 mM MgCl2, 1 mM EDTA, 1 mM DTT, 0.075% NP-40, 1x protease/phosphatase inhibitor cocktails, Roche) and incubated for 10 minutes at 4°C with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... three hearts per experimental group were homogenized into ice-cold isolation buffer (300 mM KCl, 30 mM PIPES pH 6.6, 0.5% NP-40, 1× protease inhibitor [Roche], 1× phos-stop [Roche]), followed by centrifugation at 15,000 rpm for 10 minutes in a tabletop centrifuge at 4°C to remove insoluble proteins ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with glass beads in 250 µl RIPA buffer (25mM Tris/HCl pH 7.6, 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, Roche complete protease inhibitor cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM EDTA, 0.1% NP-40, 1 mM DTT, 1 mM PMSF, 1.3 mM benzamidine, protease inhibitor tablets [Roche]) using 300 μg/mL lysozyme for 20 minutes followed by sonication ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 mM Tris-HCl [pH 7.5], 1 mM DTT, 0.1% NP-40, and 1× protease inhibitor EDTA-free mixture [Roche]) on ice for 30 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell pellets were then lysed in 1 mL of Lysis Buffer (200 mM NaCl, 25 mM HEPES, 1 mM MgCl2, 0.2 mM EDTA, 0.5% NP-40, 1× Roche complete inhibitor ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 mM Tris-HCl [pH 7.5], 1 mM DTT, 0.5% NP-40, and 1× protease inhibitor EDTA-free mixture [Roche]) on ice for 30 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... The beads were then washed 3 times with 500 µL of cold wash buffer (50 mM Tris-HCL [pH7.5], 150 mM NaCl, 0.05% NP-40, 1 mM DTT, and 1× protease inhibitor EDTA-free mixture [Roche]). RNA-protein complexes were eluted from the washed beads by incubating with 60 µL of 2× Laemmli buffer at 95 °C for 5 minutes ...
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bioRxiv - Genomics 2024Quote: ... Cell lysis was carried out by resuspending cells in complete LB1 (50 mM Hepes pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% NP-40, 0.25% Triton X-100, 1 × Roche cOmplete Mini (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... 1% NP-40 and 5% glycerol) supplemented with protease inhibitors (Roche Complete EDTA-free). Protein concentrations were determined by bicinchoninic acid assay (BCA protein assay kit ...
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bioRxiv - Cell Biology 2020Quote: ... 1% NP-40 supplemented with a cocktail of protease inhibitor tablets (Roche, Basel, Switzerland)) and incubated for 15 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... 1% Nonidet P-40) supplemented with 100x cOmplete Protease Inhibitor Cocktail (Roche, Sigma-Aldrich) and 250x PMSF Protease Inhibitor (100 mM in dry isopropanol ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.075% NP-40) supplemented with 1 Complete EDTA-free protease inhibitor cocktail (Roche), 20 mM beta-glycerophosphate ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% NP-40) containing protease inhibitor cocktail (cOmplete, EDTA-free Protease Inhibitor Cocktail – Roche). Supernatants were collected after centrifugation at 12000 RPM ...
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bioRxiv - Cancer Biology 2022Quote: ... 0,1% sodium deoxycholate and 1% NP-40 with Complete EDTA-free protease inhibitors (Roche) and sonicated 10 s on ice ...
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bioRxiv - Neuroscience 2024Quote: ... 1% NP-40) with freshly added cOmplete mini protease inhibitor cocktail (Roche, cat. # 04693124001). After incubating on ice for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1% NP-40) supplemented with 1 mM DTT and complete protease inhibitor cocktail (Roche) and sonicated for 10 min (30’’ON/30’’OFF ...
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bioRxiv - Cancer Biology 2024Quote: ... pH7.9, 1 mM EDTA, 1.5 mM MgCl2, 150 mM NaCl, 0.5% NP 40, 1 mM dithiothreitol, 1 × Roche protease inhibitor cocktail). The beads were eluted with 1xSDS sample buffer and subjected to SDS–PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... 10% glycerol, 1 mM EDTA, 1.5 mM MgCl2, 300 mM NaCl, 0.5% NP 40, 1 mM dithiothreitol, 1 × Roche protease inhibitor cocktail) for 30 min on ice to isolate the nuclear fraction ...
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bioRxiv - Cancer Biology 2024Quote: ... pH7.9, 1 mM EDTA, 1.5 mM MgCl2, 150 mM NaCl, 0.5% NP 40, 1 mM dithiothreitol, 1 × Roche protease inhibitor cocktail) and eluted with 30 µl of 2 × SDS sample buffer and subjected to western blot (WB ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.1%NP-40 (11332473001, Roche); 0.01% Digitonin (D141-500MG ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.04% NP-40 supplement (Roche), 4x Complete protease inhibitors (Roche) ...
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bioRxiv - Neuroscience 2020Quote: ... 100 mM NaCl; 1 mM EDTA, 1% Triton-X or 0.1% NP-40; supplemented with Complete Mini protease inhibitors, Roche). Cell lysates were cleared by centrifugation (3x 10 min at 20000 g at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... 0.5% NP-40 and 1 mM EDTA supplemented with 1 × protease inhibitor complete mini EDTA-free cocktail from Roche). The supernatants of cell lysates were boiled for 5 min in 1 × SDS loading buffer (6 × ...
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bioRxiv - Biochemistry 2020Quote: ... 1% v/v NP-40, 0.1% w/v SDS, 1% w/v sodium deoxycholate, 1× complete mini-protease mixture; Roche), and incubated on ice for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... diluted with an equal volume of IP buffer (150 mM HEPES-KOH pH 6.8, 150 mM K2OAc, 2 mM MgOAc, 1 mM CaCl2, 15% glycerol, 1% NP-40, 1x Roche protease inhibitor cocktail and 1 x phosSTOP cocktail ...
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bioRxiv - Microbiology 2021Quote: ... 60 mM KCl, 1 mM CaCl2, 5 mM MgCl2, 300 mM sucrose, 0.4% NP-40, 1 mM DTT, 1x Roche Complete protease inhibitors cocktail EDTA-free ...
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bioRxiv - Microbiology 2022Quote: ... cells were lysed with lysis buffer (final concentration: 50 μg ml−1 lysozyme, 0.8% NP-40, 1 × protease inhibitor (Roche), 250 U ml−1 benzonase ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5 mM MgCl2, 1 mM EDTA, 1 mM DTT, 0.1% NP-40, and a protease inhibitor cocktail tablet [Roche]). After clarifying lysates by centrifugation at 2,000 rpm for 1 min at 4 °C twice ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were prepared by resuspending cell pellets in 2 mL NP-40 buffer (50 mM Tris-HCl pH 7.8, 150 mM NaCl, 1% NP-40, 1 mM PMSF, 1x PhosSTOP [Roche]), sonication on ice (5s on/10s off for 2 mins at 40% amplitude) ...
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bioRxiv - Molecular Biology 2023Quote: ... 140 mM NaC1, 1 mM EDTA, 10% glycerol, 0.1% NP-40, 1 mM PMSF, and 1x protease inhibitor cocktail [Complete; Roche]). The cell pellet was flash frozen in liquid nitrogen with 1.5 mL of lysis buffer and grounded in a Spex Freezer Mill 6775 to a fine powder ...
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bioRxiv - Neuroscience 2022Quote: ... previously equilibrated in Wash buffer 1 (50 mM Tris-HCl pH 7.5, 150 nM NaCl, 1% NP-40, 1x protease inhibitors (Roche)) for 3 times ...
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bioRxiv - Cancer Biology 2022Quote: ... whole-cell extracts were prepared by lysing cells in buffer X (50 mM Tris pH 8.5, 250 mM NaCl, 1 mM EDTA, 1% NP-40, protease inhibitor minitablet (Roche)) and quantified using Bradford assay (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: Cell pellet was collected and re-suspended in lysis buffer (25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol, Roche complete protease inhibitor ...
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bioRxiv - Microbiology 2020Quote: ... Exponential phase cultures were inoculated 1:40 into 7H9 supplemented with kanamycin (Roche, 20ug/ml) and ATc (Sigma 100ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... 15 mM MgCl2 and 1% NP-40) supplemented with Protease Inhibitor Cocktail (Roche, Basel, Switzerland), 500 μM PMSF (serine protease inhibitor) ...
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bioRxiv - Cell Biology 2022Quote: ... containing 1% NP-40 and 0.25% sodium deoxycholate plus a complete protease inhibitor cocktail (Roche) on ice ...