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Citations for Roche :
351 - 400 of 6405 citations for AS 1517499 CAS 919486 40 1 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and 0.5% NP-40) supplied with protease inhibitors (Roche) on ice for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... The pellets were washed once with Buffer A and then further lysed with 250 μl modified RIPA buffer (1mM EDTA, 150mM NaCl, 0.1% Na-DOC, 1% NP-40, 50Mm Tris pH7.5 and Complete Roche protein inhibitors). 50U of benzonase (nuclease ...
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bioRxiv - Neuroscience 2021Quote: ... and were homogenised in TBS pH 7.4, 1% NP-40 containing phosphatase inhibitors (10mM NaF, 1mM Na3VO4) and protease inhibitors (cOmplete®, Roche). Protein concentration was adjusted to 10 mg/ml by the Bradford method (Pierce ...
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bioRxiv - Molecular Biology 2020Quote: ... Eight million HEK293T cells were lysed with 800 μL cell lysis buffer (HBS, 0.1% NP-40, 5 mM EGTA, supplemented with 1 χ protease inhibitor cocktail [Roche, Cat.# 11873580001] ...
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bioRxiv - Biochemistry 2020Quote: ... Membrane pellets were resuspended to 40 mg ml-1 in ice-cold buffer A supplemented with a protease inhibitor tablet (Roche) and 100 μM PMSF ...
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bioRxiv - Microbiology 2021Quote: ... Two days after induction, cells were lysed with lysis buffer (1x PBS, 1% NP-40, 1x protease inhibitor cocktail (Roche)) ...
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bioRxiv - Microbiology 2020Quote: ... and digested in digest buffer (RPMI supplemented with 10% FBS, 1% penicillin/streptomycin [Invitrogen], 1mg/mL collagenase D [Sigma], 1mg/mL dispase [Sigma], 40 μg/mL DNAse [Roche]) at 37° C for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.4, 200 mM NaCl, 1% NP-40, 2 mM MgCl2, 10% glycerol, NaF +Na3VO4, complete mini tablets without EDTA, Roche). Lysates were incubated on ice for 15 minutes and centrifuged at 17,000xg at 4°C for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... cells or brain tissue were lysed in mild lysis buffer (50mM Tris-HCl, 150mM NaCl and 1% NP-40) with cOmplete mini protease inhibitor cocktail (Roche) and centrifuged at 12 000g for 10 minutes at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... and cells were lysed in assay buffer (1% NP-40) supplemented with cOmplete™ mini EDTA-free protease inhibitor (Roche) and PhosSTOP phosphatase inhibitor (Roche) ...
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bioRxiv - Microbiology 2020Quote: Cells were washed once with ice-cold PBS and lysates harvested at the indicated times post infection with lysis buffer (1% NP-40, 2mM EDTA, 10% glycerol, 150mM NaCl, 50mM Tris HCl) supplemented with protease inhibitors (Roche – complete mini EDTA-free protease inhibitor ...
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bioRxiv - Immunology 2020Quote: ... supplemented with BaseMuncher nuclease (Expedion; 1 ml per 40 ml cell suspension) and Complete EDTA-free protease inhibitor mix (Roche). The extract was precleared by centrifugation ...
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bioRxiv - Biochemistry 2021Quote: Cells confluent in a 15-cm dish were washed by PBS buffer three times before being lysed on plate with 1 mL ice-cold NP-40 lysis buffer supplemented with protease inhibitor cocktail (Roche) and PhosSTOP (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were washed once with PBS and resuspended in ice-cold WCE buffer (10 mM Tris-HCl, 1% NP-40, 2 mM MgCl2, benzonase, cOmplete Protease Inhibitor Cocktail (Roche), 25 nM NEM where relevant ...
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bioRxiv - Cell Biology 2022Quote: HK cells were lysed using a lysis buffer (50mM Hepes, 130mM NaCl, 1mM DTT 1% NP-40) with complete protease inhibitor cocktail (Roche). Cell lysates were centrifuged at 13,000 rpm for 10 mins at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Thawed pellets were lysed in LB1 (50 mM HEPES-KOH pH 7.6, 140 mM NaCl, 1 mM EDTA, 10% Glycerol, 0.5% NP- 40, 0.25% Triton X-100, Roche protease inhibitor cocktail) for 20 min with rotation at 4°C and cleared by centrifugation at 300 x g for 5 min at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM EDTA, 0.1% NP-40, 1 mM DTT, 10 mM NaF, and EDTA-free protease inhibitor cocktail, Roche, Germany), and centrifugation at 20,000 × g for 3 min ...
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bioRxiv - Pathology 2024Quote: ... cDNA product was 1 to 40 diluted and real-time PCR was performed using FastStart SYBR Green Master Mix (Roche) on QuantStudio 5 (ThermoFisher ...
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bioRxiv - Genomics 2023Quote: ... and collected in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor [#11873580001, Roche] in PBS). Chromatin was sonicated with a Covaris E220 sonicator (140PIP ...
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bioRxiv - Microbiology 2023Quote: Cells were washed in PBS and harvested in lysis buffer (1% NP-40, 2mM EDTA, 10% glycerol, 150mM NaCl, 50mM Tris-HCl, protease inhibitor (Roche complete mini EDTA-free protease inhibitor) ...
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bioRxiv - Developmental Biology 2023Quote: Five whole embryos or ten explants were lysed in 1% NP-40 supplemented with protease inhibitors (Complete Mini, EDTA-free tablet (Roche), Leupeptin (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, protease inhibitor cocktail [Roche], and PhosStop [Roche]; pH 7.5) and sonicated for 30 s ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed twice with PBS and lysed using a lysis buffer (50mM Tris, pH7.4; 150mM NaCl; 1% NP-40) supplemented with protease (Roche, #11836153001) and phosphatase inhibitors (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... Thawed pellets were resuspended with 200-300 µL native lysate buffer (1% NP-40 with 1X cOmplete protease inhibitor cocktail [SKU, 11836145001, Roche] ...
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bioRxiv - Plant Biology 2022Quote: ... 10% [v/v] glycerol, 10 mM EDTA, 1% [v/v] NP-40, and complete EDTA-free protease inhibitor cocktail [Roche]). The extracts were centrifuged at 20,000 × g at 4°C for 15 min ...
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bioRxiv - Biochemistry 2023Quote: ... The pellet was resuspended in 30 mL of 40 mM Tris pH 8 supplemented with 1 tablet of EDTA-free protease inhibitor tablet (Roche), and 0.1 mM PMSF ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were lysed with lysis buffer (25 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001]). 40 µg of protein was loaded onto NuPAGE-Novex Bis-Tris 4%–12% gradient gels (Invitrogen) ...
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bioRxiv - Physiology 2023Quote: ... in PEB Buffer (150 mM NaCl, 50 mM Tris-HCl, 5mM EDTA, 1% NP-40) containing proteases (cOmplete™, Roche) and phosphatase (Phosphatase Inhibitor Cocktail 2 ...
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bioRxiv - Immunology 2024Quote: Human and murine cells were incubated in lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1 % NP-40, 5 % glycerol) containing 1X protease inhibitor cocktail (Roche) on ice ...
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bioRxiv - Developmental Biology 2024Quote: ... 1% replacement NP-40, 10 mM EDTA, 25 mM HEPES, 1 mM TCEP, 10 μM Combretastatin, 10 μM Cytochalasin D, Roche Protease Inhibitor Mini EDTA free ...
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bioRxiv - Microbiology 2021Quote: ... 1% NP40) plus an EDTA free protease inhibitor cocktail (Roche, CA, USA) as described previously [62] ...
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bioRxiv - Immunology 2021Quote: ... 1mM PMSF, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1x Roche Complete Protease Inhibitor Cocktail, and 1x Roche Phosphatase Inhibitor Cocktail). Protein concentrations were measured using a BCA kit (Pierce) ...
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bioRxiv - Cell Biology 2022Quote: ... 148.5 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 1x PhosSTOP Roche, 5x cOmplete protease inhibitor cocktail Roche, 1 mM DTT) using a cell scraper (TPP 99003) ...
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bioRxiv - Microbiology 2020Quote: ... The pellet of 100 mL was resuspended in 1 mL cold fractionation buffer (40 mM HEPES, 0.1 mM EDTA, 1x cOmplete™ Protease Inhibitor Cocktail (Roche Sigma)) and the pellet of 1 mL lysed with Bug Buster (pellet sample ...
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bioRxiv - Developmental Biology 2020Quote: ... pH 7.4, 150 mM NaCl, 1% NP-40, 2 mM EGTA, and complete protease and phosphatase inhibitors from Roche, Basel, Switzerland). Cell lysate was cleared by centrifugation ...
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bioRxiv - Genomics 2020Quote: ... cells were washed with buffer MB#1 (10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.2% NP-40, 1x Roche complete EDTA-free). Chromatin was fragmented with MNase for 10 min at 37°C and digestion was stopped with 5 mM EGTA at 65°C for 10 min ...
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bioRxiv - Immunology 2021Quote: ... and were enzymatically digested in a shaker incubator at 37°C for 40 minutes in RPMI medium containing 1 mg/ml Collagenase D (Roche-Diagnostics), 1 mg/ml hyaluronidase and 50 U/ml DNase I (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... The minced tissue is lysed in tissue lysis buffer (50 mM Tris-HCl [pH7.5], 150 mM NaCl, and 1% NP-40 (11332473001, Roche Applied Science)) for 15 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Cross-linked cells were lysed by sonication in lysis buffer (5 mM EDTA, 1% NP-40 in PBS) supplemented with 2X cOmplete™ inhibitor (Roche). Cell lysates were treated with 2 units of Turbo DNase (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... and cells were lysed by resuspension in 10mL cold NP-40 lysis buffer (150mM NaCl, 10mM Tris pH 7.2, 1% NP-40, 5mM DTT, 2mM MgCl2) containing protease inhibitors (Roche, Laval, QC) on ice for 30 minutes ...
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bioRxiv - Neuroscience 2022Quote: Protein was extracted from iNs by lysis in NP-40 lysis buffer (1% NP40, 0.5M EDTA, 5M NaCl, 1M Tris) containing cOmplete protease inhibitors and phosSTOP (Roche, Penzberg, Germany). Lysates were analyzed using the Multi-Spot Phospho (Thr 231)/Total Tau ELISA (Meso Scale Discovery ...
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bioRxiv - Microbiology 2024Quote: ... Cells were resuspended in 400 μL of lysis buffer (50 mM Tris-HCl [pH 8.0], 150 mM NaCl, 1% NP-40, cOmplete EDTA-free protease inhibitor tablets [Roche, Basel, Switzerland]), incubated for 1 h at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: ... 0.1% Triton X-100, 0.2% Nonidet P-40, 0.6 mM PMSF, and 20 µM MG132 in a Roche protease inhibitor cocktail) was added ...
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bioRxiv - Molecular Biology 2024Quote: ... then resuspended in 880 uL RIPA buffer (1x PBS, 1% NP-40, 0.5% Sodium deoxycholate, 0.1% SGS, Roche protease inhibitor cocktail), and sheared using Covaris E220 Focused-Ultrasonicator ...
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bioRxiv - Microbiology 2024Quote: ... and cells were lysed on the plates for 30 min at 4 C in Lysis Buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.5% NP-40, PhosStop, Roche Complete Protease Inhibitor). Lysates were clarified by centrifugation at 13,000 RPM ...
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bioRxiv - Immunology 2023Quote: ... Wells were pre-coated with 100 μL per well of capture anti-histone antibody contained in the Cell Death Detection ELISA kit (1:40 in 1x coating buffer; Roche 11544675001) or anti-myeloperoxidase (MPO ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclear pellets were suspended in RIPA buffer (50 mM Tris-HCl, 150 mM NaCl, 1% Nonidet P-40, 0.5% NaDeoxycholate, 0.1% SDS, 1x Roche protease inhibitor mixture) and sonicated 5 times x 5 min (30 s ON/ 30 s OFF ...
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bioRxiv - Cell Biology 2022Quote: ... and proteins were extracted in ice-cold lysis buffer (150 mM NaCl, 2 mM EDTA, 40 mM HEPES, and 1% Triton X-100) supplemented with phosphatase (Roche #04906837001) and protease inhibitor cocktail ...
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bioRxiv - Microbiology 2022Quote: ... and cells were lysed on the plates for 30 minutes at 4ºC in Lysis Buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.5% NP-40, PhosStop, Roche Complete Protease Inhibitor). Lysates were clarified by centrifugation at 13,000 RPM ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5% (w/v) DOC and 0.1% (w/v) SDS) in the presence of protease (1:40 cOmplete™ Protease Inhibitor Cocktail (Roche, #11836145001)) and phosphatase inhibitors (1:100 Phosphatase inhibitor cocktail 2 (Sigma Aldrich ...