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Citations for Roche :
101 - 150 of 420 citations for 8 16 Pyranthrenedione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... HUVEC were seeded to confluency on the microelectrodes of the E-plate (E-plate 16, Roche Applied Science). Electrical impedance readings were taken every 2 min for 5h ...
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bioRxiv - Immunology 2022Quote: ... 16S rRNA V3 and V4 regions were amplified by PCR using Kapa Hifi Hotstart Ready Mix (KAPA Biosystems) with an amplicon PCR primer set (Forward ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 mM CTP, 1 mM GTP, 0.65 mM UTP, 0.35 mM Biotin-16-UTP, pH 7.5; Roche/Sigma) and ∼40 U of either T7 (Roche/Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mM EDTA pH 8) in presence of Complete Protease Inhibitor Cocktail (Roche) and Halt Phosphatase Inhibitor Cocktail (Pierce) ...
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bioRxiv - Biochemistry 2021Quote: ... pH 8) containing one tablet of cOmplete EDTA-free Protease Inhibitor Cocktail (Roche). Cells were lysed via two passages through a French pressure cell at 1,500 psi ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the remaining 21865 reads (∼8 Mb) were submitted for assembly by Newbler (Roche). Of the 6309 obtained contigs (N50 = 663 bp) ...
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bioRxiv - Microbiology 2021Quote: ... pH 8 (buffer A) supplemented with EDTA free complete protease inhibitor tablets (Roche). The cells were lysed using a French press at 18000 psi ...
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bioRxiv - Cell Biology 2022Quote: Conjugation of protein or nanobody was performed with an 8-fold (Laminin; Roche) or 4-fold (R2-myc-his ...
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bioRxiv - Genetics 2023Quote: ... with 6-8 cycles of PCR amplification using KAPA-HiFi (Kapa Biosystems, KK2602). DNA library was sequenced using an Illumina NextSeq 500 75-cycle high output kit.
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bioRxiv - Zoology 2021Quote: ... PCR amplifications of the bacterial V3-V4 16S rRNA gene fragments were performed using KAPA HiFi HotStart ReadyMix (Roche) and the primer pair 341F (5’-CCTACGGGNGGCWGCAG-3’ ...
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bioRxiv - Microbiology 2021Quote: ... The 16S rRNA V3–V4 amplicon was amplified using a KAPA HiFi HotStart ReadyMix PCR Kit (KAPA BioSystems, USA). the amplicon PCR forward primer (5’-CCTACGGGNGGCWGCAG-3’ ...
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bioRxiv - Genomics 2022Quote: ... The BAC clone (MSMg01-38O12) was labeled by nick-translation with biotin-16-dUTP (Cat# 11093070910, Roche Applied Science) following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2019Quote: Real-time cell proliferation was measured using an E-plate 16 and a RTCA DP Analyser (xCelligence System, Roche), which uses gold microelectrodes fused to the bottom surface of a microtiter plate well ...
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bioRxiv - Molecular Biology 2022Quote: Dried peptides were re-dissolved in 212 µL of pyro-glu buffer (16 mM NaCl, 0.5 mM EDTA, 3 mM cysteamine and 50 μM aprotinin (Roche)) ...
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bioRxiv - Developmental Biology 2023Quote: ... HUVEC were seeded at a density of 60,000cells/well of the E-plate (E-plate 16, Roche Applied Science), then electrical impedance readings acquired every 2 min for 24 hr ...
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bioRxiv - Molecular Biology 2023Quote: For in vitro RNA transcription of LINC00941 the Sp6 RNA Polymerase was used according to the manufacturer’s instructions with the exception that LINC00941 was labelled with biotin-16-UTP (Roche). 2 µg of biotin-16-labelled LINC00941 were incubated with protein lysate of 3*107 keratinocytes ...
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bioRxiv - Developmental Biology 2023Quote: ... B-16 cells were collected after 48 hours of transfection and RNA was extracted using (Roche, RNA isolation Kit). Total RNA was quantified on a nanodrop (Thermofisher ...
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bioRxiv - Genomics 2023Quote: ... accession numbers OY726585 and OY726586) were indirectly labeled by PCR in the presence of biotin-16-dUTP (Roche Diagnostics) detected by streptavidin-Cy3 (Sigma–Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... or diluted to OD600 = 0.2 and treated with 8 mM DTT (Roche, Mannheim, Germany). Cells were grown for another 3 h ...
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bioRxiv - Developmental Biology 2019Quote: ... Deparaffinized and rehydrated sections were treated with 8 μg/mL of Proteinase K (Roche) in PBS for 10 min ...
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bioRxiv - Molecular Biology 2019Quote: ... 50mM Tris-HCl pH=8) with fresh EDTA-free protease inhibitor cocktail (Roche #4693132001). Protein concentration was measured using Bio-rad protein assay (#500-0006) ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were then treated with 8 U PNGase F (Roche Cat. Nr. 11365177001) at 37 °C for 3 hours followed by 19 μg/sample of trypsin (Roche Cat ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were lysed in hypotonic lysis buffer (10 mM Tris/HCl pH 8, Roche protease inhibitors ...
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bioRxiv - Cell Biology 2023Quote: ... and 50 mM Tris-Cl pH 8) supplemented with cOmplete protease inhibitor (Roche, 11836170001). Isolated proteins were flash frozen and sent to UC San Diego’s GlycoAnalytic Core for N-glycan analysis using Ultra-Performance Liquid Chromatography (UPLC ...
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bioRxiv - Microbiology 2024Quote: ... and 8 to 10 PCR cycles with the KAPA HiFi PCR kit (Kapa Biosystems) were used for amplification ...
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bioRxiv - Cell Biology 2020Quote: ... bovine serum albumin in TBS-T and incubated for 16 h at 4°C: mouse anti-GFP (1:1000) (Roche), sheep anti-CK1α (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Pre-amplification of the V3V4 region of 16S rRNA was performed by PCR in triplicate using 2xKAPA HiFi Hot Start ReadyMix (Roche) using primer pairs containing adaptor sequences ...
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Elevated Hoxb5b expands vagal neural crest pool and blocks enteric neuronal development in zebrafishbioRxiv - Developmental Biology 2021Quote: ... Probed embryos were blocked for 1-2 hours at ambient temperature in 5% Goat sera in PBST before detection overnight (∼16 hours) at 4°C using an anti-Digoxigenin-Fab fragments conjugated to Alkaline Phosphatase enzymes (1:1000 dilution, Roche) in 5% Goat Sera in PBST ...
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bioRxiv - Biochemistry 2020Quote: ... Biotin-labeled CMV-RLuc-EV715’UTR (wild type or CCC mutant)-FLUC and biotin-labeled SL2 (wild type or CCC mutant) were synthesized using biotin-16-UTP (Roche). Binding reaction and pull-down were carried out as described5 ...
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bioRxiv - Microbiology 2021Quote: ... The mixture was re-incubated 20 min at 75°C and the purified DNA product further incubated overnight at 16°C with a T4 DNA ligase (Roche).
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bioRxiv - Cancer Biology 2020Quote: ... 1·104 cells per well were seeded onto E-Plates 16 and analysed in the xCELLigence RTCA DP system (Roche) for 48 h in quadruplicates.
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bioRxiv - Cell Biology 2021Quote: ... and pBlueScript II KS reverse (5’-GCT GGG TCT CGT GGT TTC CCT TTA GTG AGG GTT AAT TG) primers and either 60 µM biotin-16-dUTP or digoxigenin-11-dUTP (Roche). This results in a 5 kb DNA tether which can be attached to a streptavidin coated magnetic bead at one end and an anti-digoxigenin coverslip surface at the other.
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bioRxiv - Biophysics 2021Quote: ... including 200 μM final concentration of each dNTP (G,C,T,A) and 10 µM Bio-16-dUTP or Dig-11-dUTP (all from Roche). Labelled handles specifically bind either to a glass surface covered with anti-digoxigenins or to superparamagnetic beads covered with streptavidin ...
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bioRxiv - Cancer Biology 2019Quote: ... The two DNA handles (510bp) labeled by multiple digoxigenin and biotin are generated by mixing biotin-16-dUTP and digoxigenin-11-dUTP nucleotides (Roche) with dNTP solution mix in the respective PCR reaction ...
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bioRxiv - Microbiology 2021Quote: We initially amplified the region of bacterial 16S-23S rRNA gene and fungi full length ITS region with Kapa Hifi DNA polymerase (Roche) and EX Taq DNA polymerase (Takara Bio) ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 μL of 25 × diluted oligonucleotides and 50 ng of the digested and dephosphorylated vector were ligated overnight at 16°C using T4 Ligase (Roche), and transformed into XL10-Gold E ...
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bioRxiv - Genomics 2021Quote: ... The enriched libraries were amplified by a second round of LM-PCR with 16 cycles using Kapa Hifi Uracil+ polymerase (Roche) and purified with AMPure XP magnetic beads ...
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bioRxiv - Molecular Biology 2022Quote: ... the fragment of interest was synthesized with Q5 DNA polymerase and handles were synthesized with Taq using either biotin-16-dUTP (11093070910, Roche) or digoxigenin-11-dUTP (11093088910 ...
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bioRxiv - Genomics 2023Quote: ... The resulting UMI-labeled 16S dsDNA was then amplified using indexed p5/p7 primers and KAPA HiFi HotStart ReadyMix (Roche) (95°C for 3 minutes followed by 25 cycles of 98°C for 20 seconds ...
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bioRxiv - Molecular Biology 2023Quote: 100 pmol gapmers (Supplemental Table S4) were labeled with biotin-16-ddUTP (Jena) and a 2nd generation DIG-oligonucleotide 3’end-labeling kit (Roche) using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer ...
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bioRxiv - Genomics 2023Quote: ... GenBank accession number Z69354.1) and BlSat1 (Hong Ha 2018) were labelled by PCR in the presence of biotin-16-dUTP (Roche Diagnostics) detected by streptavidin-Cy3 (Sigma–Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by primary antibody labelling (16-120 min) and signal detection carried out with the OptiView DAB IHC Detection Kit (VENTANA/Roche), as described previously (33) ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 mM EDTA at pH 8) containing protease inhibitors (cOmplete mini EDTA-free tablets, Roche) for 30 mins on ice ...
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bioRxiv - Genomics 2019Quote: ... and 8–10 kb) were constructed with the KAPA Hyper Prep Kit (Kapa Biosystems, Roche) and Nextera Mate Pair Library Preparation Kit ...
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bioRxiv - Genomics 2019Quote: ... and 8–10 kb) were constructed with the KAPA Hyper Prep Kit (Kapa Biosystems, Roche) and Nextera Mate Pair Library Preparation Kit ...
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bioRxiv - Genomics 2021Quote: ... The ligated products were enriched with 8-10 cycles of PCR (HiFi premix, KAPA biosystems) and size selected to 200-500 bp with AMPure XP beads (Agencourt) ...
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bioRxiv - Synthetic Biology 2021Quote: ... with 8 mL water plus one cOmplete™ Mini EDTA-free 11836170001 (Roche, Basel, Switzerland) protease inhibitor tablet) ...
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bioRxiv - Cell Biology 2021Quote: ... pH 8) containing 1 mM PMSF and Complete™ EDTA free protease inhibitor cocktail (Roche). Nuclei were removed by centrifugation for 1 min at 6,000 rpm ...
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bioRxiv - Immunology 2021Quote: ... and subsequently digested for 8 minutes with the Digestion Buffer (0.2mg/mL Liberase™ Roche Diagnostics ...
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bioRxiv - Molecular Biology 2022Quote: ... 8 M urea) containing protease-inhibitor mix (Complete EDTA-free; Roche # 11 873 580 001), and frozen at -70°C ...