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Citations for Roche :
1401 - 1450 of 2319 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... 10 mM NaCl, 3 mM MgCl2, 0.5 mM spermidine, 0.2 mM spermine, 0.01% Triton-X, 1x Roche complete protease inhibitors ...
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bioRxiv - Plant Biology 2023Quote: ... in 384-wells plates with a total volume of 10 uL using Light Cycler 480 apparatus (Roche). mRNA abundance was compared to two reference genes EXP (AT4G26410 ...
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bioRxiv - Neuroscience 2023Quote: Culture media collected from the treatments was supplemented with a 10× concentrated solution of PI cocktail (Roche) at a 9:1 conditioned:fresh media ratio (i.e. ...
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bioRxiv - Microbiology 2023Quote: ... 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 1 mM DTT, 0.5 mM AEBSF, 1X Roche protease inhibitor ...
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bioRxiv - Molecular Biology 2023Quote: ... reactions were set up at 10 µl reaction volume using LightCycler 480 SYBR Green Master Mix (Roche) and performed using the LightCycler 480 Real-Time PCR Instrument (Roche) ...
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bioRxiv - Biochemistry 2023Quote: ... The extracted total RNA was treated with 140 U RNase-free DNase I (Roche, 10 U/μL) at 37℃ for 30 min ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative PCR (qPCR) was performed in 10 µL reactions using KAPA SYBR Fast qPCR Kit (KAPA BIOSYSTEMS). Expression levels were calculated using the 2–ΔΔCt method ...
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bioRxiv - Molecular Biology 2022Quote: ... and 10 w/v glycerol) supplemented with 5x protease EDTA-free inhibitor tablets (Roche, cat. No. 11836153001), and frozen by dropping the suspended yeast into liquid nitrogen ...
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bioRxiv - Immunology 2022Quote: ... C57BL/6NCrl mice were given either 2% (w/v) Glucose water or 10% (v/v) Ethanol (Roche) and 2% (w/v ...
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bioRxiv - Molecular Biology 2022Quote: ... The nuclear pellet was washed gently with 10 volumes of Buffer A containing 1x CPI (Roche, #11836145001), 0.5 mM DTT and 0.15 % NP40 and centrifuged for 5 min at 3,200 g at 4°C min ...
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bioRxiv - Microbiology 2022Quote: ... and lentivirus vectors at a 2:8:10 μg ratio to HEK293T cells using Xtreme-Gene9 (Roche) and lentivirus-containing supernatants were collected 72hr post-transfection ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... Cells were then resuspended in 1 mL of ice cold NE1 Buffer (20 mM HEPES-KOH pH 7.9, 10 mM KCl, 0.5 mM Spermidine, 0.1% TritonX-100, 20% Glycerol, with Roche complete EDTA-free protease inhibitor tablet) ...
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bioRxiv - Neuroscience 2023Quote: ... They were homogenized in 10 μl/mg tissue 0.32 M sucrose containing protease inhibitor (cOmplete Mini, Roche) then centrifuged at 17000 g at 4°C for 30 min ...
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bioRxiv - Genomics 2023Quote: ... (d) Remaining 9/10 of sample is immunoprecipitated with 1:1000 anti-HA antibody (0.1mg/mL Roche Rat Anti-HA High Affinity [11867423001] ...
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bioRxiv - Cell Biology 2023Quote: ... or Nunc Glass Base Dishes (Thermo Fischer Scientific) coated with sterile 10 µg ml−1 fibronectin (Roche). Supplier and identifier for all reagents are listed in Table 4 ...
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Label-free structural imaging of plant roots and microbes using third-harmonic generation microscopybioRxiv - Plant Biology 2024Quote: ... followed by immersion in 10% (v/v) household bleach containing 0.1% Triton X-100 (Roche Diagnostics GmbH) were stratified in distilled water at 4 °C for 2 days ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative PCR reactions were performed in 10 µl with Kapa SYBR Fast qPCR universal mix (Kapa Biosystems) with 100 nM of each PCR primer per reaction in a Roche LightCycler 96 with the following program ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated for 30 min at 37 °C with mouse anti-BrdU (11296736, Roche. 1/10) then washed before incubation at room temperature for 3 h with the primary antibody anti-myosin heavy chain (MF20 ...
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bioRxiv - Microbiology 2024Quote: ... and then resuspended in 1 mL of ice cold NE1 Buffer (20 mM HEPES-KOH pH 7.9, 10 mM KCl, 0.5 mM Spermidine, 0.1% TritonX-100, 20% Glycerol, with Roche complete EDTA-free protease inhibitor tablet) ...
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bioRxiv - Immunology 2024Quote: ... nuclei were prepared using a nuclear extraction buffer (20 mM HEPES pH 7.9, 10 mM KCl, 0.1% Triton X-100, 20% Glycerol, 0.5 mM Spermidine, 1x Roche cOmplete™ ...
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bioRxiv - Immunology 2024Quote: ... in a shaking incubator before digestion in 10%FCS/RPMI1640 with 0.625mg ml−1 collagenase D (Roche), 0.425mg ml−1 collagenase V (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were subsequently resuspended in 5 ml of cold Buffer A (10 mM Hepes pH 7.9, 10 mM KCl, 1.5 mMMgCl2, 0.5 mM DTT, EDTA-free protease inhibitors, Roche) and incubated 15 min on ice ...
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bioRxiv - Genomics 2024Quote: ... ligated to IDT 8 or 10 dual index adaptors and amplified using KAPA HyperPrep Kit (Roche, KK8504). Libraries were enriched for exomic sequences by hybridization with NimbleGen SeqCap EZ Exome + UTR (Roche ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with 10 μg/mL DNase I and 1x cOmplete™ EDTA-free protease inhibitor cocktail (Roche). Cell lysis and membrane solubilization was carried out using a Dounce homogenizer and incubated at 4°C for two hours ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% protease and phosphatase inhibitors (cat. no. A32957, Thermo Fisher Scientific, cat. no.11836153001, Roche). SDS-polyacrylamid gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cell Biology 2024Quote: ... pH 7.5, 10 mM KCl, 0.1% Triton X-100, 20 mM Glycerol, 0.5 mM Spermidine, 1X Roche cOmplete Mini EDTA-free Protease Inhibitor (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% (v/v) Triton X-100 and 10% (v/v) of protease and phosphatase inhibitor cocktail (Roche). After sample denaturation and electrophoresis ...
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bioRxiv - Cancer Biology 2024Quote: ... and directly placed in freshly-made pepsin working solution (20 µl of 10 mg/µl Pepsin, Roche, Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: Cell suspension (1 million cells/ml) was mixed with 6x SDS loading buffer and 10 mM DTT (Roche) and incubated at 95 °C for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown in 10 μM BrdU:BrdC (3:1) for 16–20 h before incubation with 0.1 μg/mL colcemid (Roche) for 2-3 h ...
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bioRxiv - Developmental Biology 2021Quote: ... Pellets were resuspended in nuclear lysis buffer (20mM HEPES pH 7.9, 0.4M NaCl, 1mM EDTA, 10% Glycerol, Protease Inhibitor (1X) (Roche)) and sonicated at amplitude 40 for 10 seconds twice ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 mM EDTA, 300 mM NaCl, 10 mM Tris.HCl [pH 8.0], 1 mM EDTA, 1x Complete protease inhibitor [Roche] ...
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bioRxiv - Biophysics 2022Quote: ... 300 mM NaCl and 10 mM imidazole pH 8.0) containing one tablet of cOmplete protease inhibitor cocktail (Roche), and lysed by sonication ...
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bioRxiv - Biochemistry 2022Quote: ... 4 mM MgCl2, 10 % v/v glycerol, 140 mM NaCl, 0.1 mM EDTA, 0.5 % Tween20, 1 mM DTT and Roche cOmplete-Ultra EDTA free protease inhibitor) ...
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bioRxiv - Neuroscience 2020Quote: ... Dissected hippocampi were dissolved in lysis buffer (1M Tris-Cl, pH 7.5; 6M NaCl; 10% SDS; 0.5M EDTA; Triton-X 100; Phosphatase Inhibitor #3, Roche, #05-892-970-001 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% NP40 and 10 mM Tris (pH 7.5)) supplemented with cOmplete EDTA-free protease inhibitor (Roche, Basel, Switzerland). Samples were incubated on ice for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... The brain tissue was then homogenized on ice using a dounce homogenizer and diluted to 5 mL/g in homogenization buffer with RNase inhibitors (10mM Tris-HCl pH7.4, 0.8M NaCl, 1mM EGTA, 10% sucrose, 1X Roche protease inhibitor ...
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bioRxiv - Genomics 2021Quote: ... Cells were lysed by the addition of 1000 μL lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl, pH 8.0, and 0.25× protease inhibitor cocktail [Roche]). Cells were sonicated on ice to generate an average DNA fragment size of ∼250 bp ...
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bioRxiv - Neuroscience 2020Quote: Hippocampi were collected 24 h after KA treatment and protein extracts were obtained by processing samples with lysis buffer (Tris-Base 0.5 M, pH 7.4; SDS 10 %) containing phosphatase and proteinase inhibitors (Roche). Lysates were clarified by centrifugation at 14000 x g for 10 min and protein concentration was measured using the bicinchoninic acid assay ...
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bioRxiv - Molecular Biology 2020Quote: ... 110 mM KOAc, 0.5% Triton X-100, 0.1% Tween 20, 10 mM MnCl2, 1x proteinase inhibitors (complete, EDTA-free (Roche)) ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.9; 10 mM KCl; 0.1% Triton X-100; 20% Glycerol; 0.5mM spermidine; 1x complete protease inhibitor [Roche # 11836170001]), collecting by centrifugation (600 g ...
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bioRxiv - Neuroscience 2021Quote: Real-time PCR was carried out in a 10 μL reaction using SYBR Green PCR Master Mix (Roche) using 1ng/μL of cDNA according to the manufacturer’s protocol with a QuantStudio 12 K Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... 10 mM EDTA) supplemented with protease inhibitors (complete mini EDTA-free, Roche, 1 tablet / 50 mL lysis buffer). The lysed neurons were mixed with 4X Laemmli sample buffer and heated at 70°C for 10 minutes (stored at −20°C until use) ...
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bioRxiv - Plant Biology 2021Quote: Root tissue was ground in IP buffer (50mM Tris-HCL, pH7.5, 150mM NaCL, 10% glycerol, 0.1% NP40) containing cOmplete protease inhibitor (Roche). After clearing lysates at 12,000x g (10 min) ...
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bioRxiv - Plant Biology 2021Quote: ... 2 * 107 cells were pelleted and resuspended in 1 ml of lysis buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA) including 0.25x protease inhibitor (Roche)) ...
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bioRxiv - Microbiology 2021Quote: ... Pellets were resuspended in 1 ml buffer (50 mM Tris, pH 8.0, 10 mM DTT, 0.1% SDS, 100 μM PMSF, phos-stop tablet [Roche]), and cells were lysed by 2 minutes of vortexing with zirconia beads (BioSpec Products) ...
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bioRxiv - Systems Biology 2020Quote: ... We ran 10 μl reactions using the LightCycler 480 platform and the SYBR Green I Master kit (Roche Diagnostics Corporation ...
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bioRxiv - Microbiology 2020Quote: ... Cells were seeded in 10 cm tissue-culture treated dishes and transfected with FuGENE 6® reagent (Roche) for 24 hr ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μl 1xTaqMan Gene Expression Assay primer and 10 μl 1x FastStart Universal Probe Master-mix (Roche, 04913957001) were applied ...
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An optimized ChIP-Seq framework for profiling of histone modifications in Chromochloris zofingiensisbioRxiv - Genomics 2021Quote: ... The cell pellet was resuspended in 1 mL ChIP lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-Cl, pH 8.0, and 0.25 x protease inhibitor cocktail [Roche]) and transferred to Beckman Coulter 4mL Polycarbonate Thick Wall Centrifuge Tubes (13 × 64 mm) ...