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Citations for Roche :
1401 - 1450 of 2345 citations for 2 Methoxycarbonyl 4 methylthiophene 3 diazonium tetrafluoroborate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Nuclei were counterstained with 5 ng/ml of 4,6–diamidino-2-phenylindole dihydrochloride (DAPI; Roche). Coverslips were mounted on slides with Fluoromount G (Electron Microscopy Sciences ...
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β-amyloid−driven synaptic depression requires PDZ protein interaction at AMPA-receptor subunit GluA3bioRxiv - Neuroscience 2021Quote: ... 150 mM NaCl) containing 2% Triton X-100 and EDTA-free protease inhibitor cocktail (Roche). The resulting samples were incubated for 1 h at 4°C and spun down twice at 20,800 x g for 10 min at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Cells were cultured in complete media with 30 IU/mL IL-2 (Roche, 202-IL). Media containing IL-2 was replenished daily for 10 days ...
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bioRxiv - Immunology 2020Quote: ... The lungs were removed and infiltrated with 2 mg/ml collagenase D (Roche, Indianapolis, Indiana) and 0.2 mg/ml DNase I (Roche ...
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bioRxiv - Microbiology 2022Quote: ... one of which was treated with 2 U of PNGase F (Roche Cat. Nr. 11365177001) at 37 °C for 1 hour ...
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bioRxiv - Physiology 2022Quote: ... pyruvate supplemented with 2% Bovine Serum Albumin Fraction V Fatty acid free (Roche, USA/UK), 50 µM 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2020Quote: ... 9ml of cold wash buffer (PBS, BSA 2% and 0.2U/μl RNase inhibitor from Roche) was added and the lysate was dounced with 10 strokes of loose pestle avoiding too much pressure and air bubbles ...
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bioRxiv - Biochemistry 2021Quote: ... mRNA expression analysis was determined using KAPA SYBR Fast Master Mix (2×) Universal (KAPA Biosystems) in an Eco Real-Time PCR System (Illumina) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 2% Triton X-100 and 274 mM NaCl) containing a cocktail of protease inhibitors (Roche). Cells were lysed for 20 min on ice and centrifuged for 15 min at 17,400 r.p.m ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 2 uL of 50 mM MgSO4 and dNTPs (Roche#11-581-295-001) and thermal cycler program ...
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bioRxiv - Biophysics 2020Quote: ... 5 mM MgCl2 (buffer B) containing 2 mM PMSF and Complete protease inhibitor cocktail (Roche). After centrifugation ...
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bioRxiv - Genetics 2021Quote: ... 2% Triton X-100 in RIPA homogenizing buffer) supplemented with a protease-inhibitor cocktail (Roche) was used to resuspend the cells ...
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bioRxiv - Microbiology 2020Quote: ... Nuclei were counterstained with 5 ng/ml of 4,6–diamidino-2-phenylindole dihydrochloride (DAPI; Roche). Coverslips were mounted on slides with Fluoromount G (Electron Microscopy Sciences ...
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bioRxiv - Immunology 2020Quote: ... diluted 2-fold with PBMC culture media and supplemented with 10% WST-1 reagent (Roche) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and the RNA was subsequently precipitated in 2-propanol and 20 μg/μl glycogen (Roche) overnight at −20 °C ...
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bioRxiv - Cell Biology 2022Quote: ... femurs were cut roughly and incubated with 2 Wunsch units of LiberaseTM (Sigma/Roche 5401127001) and 1 mg of Pronase (Sigma/Roche 10165921001 ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mM PMSF) supplemented with 1 protease inhibitor cocktail tablet (cOmplete-EDTA free, Roche, #11836170001) to a final volume of 50 mL ...
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bioRxiv - Microbiology 2024Quote: ... 2 mM beta-mercaptoethanol) in the presence of a protease inhibitor cocktail (Roche, Basel, Switzerland). These mixtures were incubated on ice for 30 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... was amplified using F3 and R3 primers (Supp. Table 2) and KAPA-Hifi polymerase (Roche). Following a 1X SPRIselect bead DNA cleanup (Beckman Coulter) ...
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bioRxiv - Cell Biology 2024Quote: ... the eyes were incubated with 2% Dispase II (neutral protease; Roche Applied Science, Indianapolis, IN) in Hank’s balanced salt solution (HBSS ...
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bioRxiv - Immunology 2024Quote: ... 2 mL aliquots of peripheral blood were treated with 30mL of RBC lysis buffer (Roche) for 5-8 minutes at room temperature with gentle mixing ...
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bioRxiv - Biophysics 2023Quote: ... 2 mM PMSF) supplemented with one protease inhibitor cocktail tablet (cOmplete-EDTA free, Roche, #11836170001) to a final volume of 50 mL ...
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bioRxiv - Immunology 2023Quote: Anti-nucleocapsid IgG was measured using the Elecsys Anti-SARS-COV-2 assay (Roche; 09203095190) run on a Cobas e411 analyser (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... 2 ng of cDNA was amplified per reaction in a 364-well plate (4729749001, Roche) with LightCycler® 480 SYBR Green I Master (4887352001 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with 2 mL of pre-equilibrated Protein C affinity resin (Roche) for 3h at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... was diluted 1 in 50 in solution consisting of 2 % blocking reagent (Roche ref 11096176001), 1 % goat serum (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... 2 mM TCEP and 5 % glycerol) supplemented with cOmplete EDTA-free protease inhibitor complex (Roche) and ribonuclease A (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... 0.5 μg/mL DAPI (4,6-diamidino-2-phenylindole, F. Hoffmann-La Roche, Natley, NJ, USA) was added to counterstain the nuclei ...
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bioRxiv - Immunology 2023Quote: ... The tissue was then digested with Liberase Blendzyme 2 (0.2 mg/ml, Roche Applied Science) and DNase I (20 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... Cells were stimulated in complete RPMI-10 supplemented with 50U/mL hIL-2 (Roche 11011456001), 2.5ng/mL TGFβ (R&D 240-B-10) ...
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bioRxiv - Microbiology 2024Quote: ... 25 wt% sucrose and 2 mg/mL lysozyme (from egg white, 10837059001 Roche, Basel, Switzerland) and incubated for 30 min at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... The slides were counterstained with 4,6-diamidino-2-phenylindole (DAPI, 1 μg/ml; Roche, Switzerland) for 20 min ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 2 mM activated sodium orthovanadate (Na3VO4) and protease and phosphatase inhibitor cocktails (Roche). Lysates could be stored at -80°C ...
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bioRxiv - Neuroscience 2024Quote: ... 2 ng of cDNA was added as template to SyberGreen master mix (Roche, Basel, Switzerland) and 250 nM primers (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... Sample were incubated for 2 hours at 37°C before addition of DNAse (Roche, # 04716728001) for 15 min and 0.2M EDTA to stop reactions ...
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bioRxiv - Immunology 2024Quote: ... Cells in each well were co-transfected (Day 2) using X-tremeGENE™ HP (Roche) with 0.8 µg of the appropriate lentiviral transfer plasmid encoding an antigen receptor (1G4 TCR or c259 TCR ...
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bioRxiv - Genomics 2024Quote: ... and eggs were resuspended in a pronase solution (from Streptomices griseus, ROCHE, 2 mg/ml), leaving the fourth dish as control ...
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bioRxiv - Bioengineering 2024Quote: ... 2 mM of MgCl2) with one protease inhibitor cocktail tablet (Mini EDTA-free, Roche, Merck) per 10 ml ...
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bioRxiv - Developmental Biology 2020Quote: ... 5% Normal Goat Serum) for 3 h at room temperature and then incubated overnight with alkaline phosphatase-conjugated anti-DIG Fab fragments (Roche, 1: 2,000) at 4°C on a shaking platform ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were lysed in 50 μl of Lysis Buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40, 1x Roche Complete protease inhibitors cocktail) by pipetting up and down ...
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bioRxiv - Developmental Biology 2021Quote: ... The DIG-labeled 3’UTR of Emi2 and cyclin B1 RNA probes were synthesized with a DIG-RNA-labeling kit (Roche Molecular Biochemicals). Two μg of probes was incubated with purified Flag-tagged proteins for 15 min ...
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bioRxiv - Neuroscience 2020Quote: ... 1996) on 16 µm cryosections at post-natal day 3 (P3) using anti-digoxigenin antibody (Sheep polyclonal, 1:1000, Roche, 11093274910, RRID:AB_514497). Two days exposure to alkaline phosphatase (AP ...
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bioRxiv - Cancer Biology 2020Quote: ... N3500], 50 mM of Tris [pH 7.5], 150 mM of NaCl, 3 mM of MgCl2, 1X protease inhibitors [Roche, Mannheim, Germany; 05892953001]). Protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... a 6.7 kb DNA fragment starting 1 kb upstream of murine Gnrhr exon 3 was amplified by PCR using the Expand Long Template PCR System (Roche, Basel, Switzerland) from 129SvEv genomic DNA using primers incorporating 5’ XmaI and 3’ NotI restriction enzyme sites (Table 1) ...
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bioRxiv - Cell Biology 2021Quote: Total protein from cells or mouse tissues (n=3 per genotype) were extracted using the M-PER protein lysis buffer (ThermoScientific, Beverly, MA) containing protease inhibitors (Roche, Indianapolis, IN). Approximately 25 μg of total protein was electrophoresed on 4-12% SDS-PAGE gels and transferred to PVDF membranes ...
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bioRxiv - Physiology 2022Quote: ... n=3/genotype) were used for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) to visualize cell apoptosis/death (Roche, Basel, Switzerland). Sections were counterstained with DAPI and visualized using epifluorescence (Nikon Eclipse Ni E800) ...
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bioRxiv - Microbiology 2022Quote: ... 45 cycles of 95°C for 3 s and 60°C for 30 s using the LightCycler 96 system (Roche, Basel, Switzerland) or the StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genomics 2022Quote: ... Empty/filled PCRs (combining 5′ and 3′ flanking primers) and full-length PCRs (using junction-spanning primers) were performed using an Expand Long Range dNTPack (Roche, Cat#: 4829034001). Reaction mixes contained 5μL 5× Expand Long Range Buffer with 12.5mM MgCl2 ...
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bioRxiv - Immunology 2020Quote: ... Membranes were blocked for 1 h with 3% (w/v) non-fat milk powder in PBS/0.1% (v/v) Tween (PBST) (CALR, 10% Roche WBR in PBST) and incubated with primary antibody (Supplementary Table III ...
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bioRxiv - Genetics 2021Quote: ... The T7 promoter was added to the 5’ and 3’ ends to synthesize the sense and anti-sense probes using the DIG RNA Labeling Kit (Roche, Cat# 11175025910). In situ hybridization was performed as previously described [65,66] with minor modifications ...