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Citations for Roche :
1351 - 1400 of 1612 citations for IL 5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the supernatant was incubated with 5% v/v pre-immune rabbit sera and 20 μl of protein G-beads (Roche) for 1 h on a rotator at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were then washed 2 × 5 min at 4 °C with cold 1 × PBS containing protease inhibitor cocktail (Roche, #4693132001), snap frozen and stored at −80°C until awaiting further processing ...
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bioRxiv - Cell Biology 2023Quote: ... 50 µl of co-IVT protein was added along with 5 μL (bed volume) of washed HA-beads (11815016001, Roche) in 300 μL binding buffer (25 mM HEPES-NaOH [pH 7.5] ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μL of sample were used in a reaction mix containing 1 μL 10 μM of each primer (0.5 μM final) and 5 μL 2x Light Cycler SYBR Green LightCycler® 480 SYBR Green I Master (Roche). For telomere amplification ...
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bioRxiv - Immunology 2023Quote: ... the common bile duct was clamped and the pancreatic duct was perfused with 3-5 mL solution of collagenase P in HBSS-1% HEPES (Roche). The pancreas was then harvested and transferred to a 50mL conical tube containing 5mL of collagenase P solution and kept on ice until all organs were collected ...
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bioRxiv - Genetics 2023Quote: DNA fragments of an Alu sequence with T7 promoter on the 5’ end (ssAlu) or both ends (dsAlu) were in vitro transcribed with the biotin RNA-labeling mix (Roche) and T7 transcription kit (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... blocked for 1 h in blocking reagent (100 mM Tris HCL pH 8; 150 mM NaCL; 5 g/L Blocking Reagent (#11096176001, Roche)) and treated for 1.5 h with primary antibody diluted in blocking reagent (NF-κB p65/RELA ...
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bioRxiv - Neuroscience 2023Quote: ... slides were incubated at 37oC in a staining solution containing nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate (Roche) for 20-24 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were lysed with lysis buffer (25 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001]). 40 µg of protein was loaded onto NuPAGE-Novex Bis-Tris 4%–12% gradient gels (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... 150 mM NaCl, 5 mM EDTA, 0.5 % NP-40, 0.1 % Triton X-100, complete protease inhibitor cocktail [Roche] added fresh) for 10 min on ice ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.1% Tween 20 in water) and incubated in NBT/BCIP (nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate) solution (Roche).
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bioRxiv - Biochemistry 2023Quote: ... Pellets were resuspended in binding buffer (50 mM HEPES pH 7.4, 150 mM NaCl, 5 mM TCEP) supplemented with protease inhibitors (Roche cOmplete), sonicated ...
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bioRxiv - Biochemistry 2023Quote: ... cell pellets were resuspended in HR buffer (50 mM Tris-HCl, 5 mM MgCl2, 250 mM sucrose, 2 mM TCEP and protease inhibitor t (Roche), pH 7.4) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed by sonication and French Press in purification buffer (50mM sodium phosphate buffer pH 7, 300 mM NaCl) supplemented with 5 ug/mL DNaseI (Roche), 5ug/mL RNAse (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were blocked in 5% low-fat milk in TBS-T for 2 hours and probed with 1:5000-diluted mouse anti-GFP (Roche) or rabbit anti-ATG8 (Agrisera ...
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bioRxiv - Plant Biology 2023Quote: ... membranes were blocked with 5% skim milk and then incubated in the primary anti-GFP mouse monoclonal antibody (Roche, 11814460001), followed by goat anti-mouse HRP conjugate (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed with protein lysis buffer (50 mM Tris-HCl, 250 mM NaCl, 5 mM EDTA, 1 mM Mg2Cl, 1% NP40 and supplemented with protease inhibitor cocktail, Roche), incubated on ice for 20 minutes and spun at 10,000 g for 10 min at 4 °C ...
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bioRxiv - Pathology 2023Quote: ... Bound alkaline phosphatase was visualized with nitroblue tetrazolium chloride and 5-bromo-4-chloro-3-indolyl-phosphate (NBT/BCIP; 11681451001, Roche). The reaction was stopped by incubation in buffer 4 (10 mM Tris and 1 mM EDTA ...
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bioRxiv - Systems Biology 2023Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting cDNAs were amplified with primers DP3 and DP5 (5’-GTTCAGAGTTCTACAGTCCGACGATC-3’, 0.5 μM) and KAPA Hifi HotStart DNA polymerase (Roche, KK2601) to the optimal amplification point ...
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bioRxiv - Immunology 2023Quote: Whole lung single cell suspensions were prepared by harvesting lung lobes into 5 ml HBSS with 40µl Liberase Tm (0.1 U/ml, Roche, Cat# 5401127001) and 20µl DNAse 1 (10mg/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... Larvae were dissociated using a combination of enzymatic disruption using Liberase (Roche, 05 401 119 001, 5 μg/mL final), DNaseI (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed with protein lysis buffer (50 mM Tris-HCl, 250 mM NaCl, 5 mM EDTA, 1 mM Mg2Cl, 1% NP40 and supplemented with protease inhibitor cocktail, Roche), incubated on ice for 20 minutes and spun at 10,000 g for 10 min at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... 100 mM NaCl, 20 mM Imidazole pH 7.5, 3 mM MgCl2, 100 µM EDTA, 5 mM β-Mercapoethanol, 20 µM GDP, Roche cOmplete protease inhibitor cocktail and DNAse I ...
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bioRxiv - Neuroscience 2023Quote: ... The larvae were then incubated overnight at 4°C with anti-Dig-AP (1:2000 in 5% normal goat serum, 11093274910, Roche) and washed before detecting alkaline phosphatase using NBP/BCIP(Roche ...
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bioRxiv - Cell Biology 2023Quote: Decapsulated testis extract was re-suspended into 1 x PBS Lysis buffer (1 x PBS, 0.01% NP-40, 5% Glycerol, 150mM NaCl, 1 x Roche cOmplete) and sonicated for 20 s at 22% amplitude in cycles of 0.4 s on and 0.2 s off ...
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bioRxiv - Cell Biology 2023Quote: ... 50 mM Tris pH 8, 450 mM NaCl, 1% CHAPS, 20 mM MgCl2, 5 mM ATP, 1 mM Dithiothreitol [DTT], 1X Roche Protease Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining extract was centrifuged at 2,000 rpm for 5 minutes and re-suspended in 1 x PBS Lysis buffer (1 x PBS, 0.01% NP-40, 5% Glycerol, 150mM NaCl, 1 x Roche cOmplete), sonicated and stored at -20°C for downstream applications.
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bioRxiv - Molecular Biology 2023Quote: ... The signals were detected by 4-nitro-blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP, Roche Diagnostics) in a humidified container for 72 h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were incubated with blocking solution (5% skimmed milk in 1X PBS) followed by overnight incubation with respective primary antibodies (anti HA rat 1:1000 (Roche), anti BiP rabbit 1:5000 in cold room and 1 hr incubation with respective secondary antibodies (1:10000 ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM MgCl2, 0.5 mM β-mercaptoethanol, 1 mM ATP, 1% IGEPAL, 5% glycerol, 3 U/ml Benzonase and Roche Complete EDTA-free protease inhibitor ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then resuspended in cold lysis buffer (10 mM Tris, pH 7.5, 10 mM NaCl, 5 mM MgCl2, 1 mM EGTA, Protease Inhibitor (Roche, 04693159001)) ...
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bioRxiv - Biochemistry 2023Quote: ... and lysed with sonication in lysis buffer (50mM Hepes, 500mM NaCl, 1% Tween20, 5% Glycerol, half a tablet of cOmplete EDTA-free protease inhibitor cocktail [Roche]). The suspension was sonicated at 150J in intervals of 1.5secs for 20 sec total in ice ...
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bioRxiv - Plant Biology 2024Quote: The pulverized tissue was incubated in 30 mL of buffer 1 (400 mM sucrose, 10 mM Tris-HCl pH 8.0, 10 mM MgCl2, 5 mM β-mercaptoethanol, 1 mM PMSF, 1× Roche cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.44 M sucrose, 1.25 % Ficoll, 2.5% Dextran, 10 mM MgCl2, 0.5% Triton X-100, 5 mM DTT, 1x Roche protease inhibitor mixture), filtered through two layers of Miracloth ...
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bioRxiv - Molecular Biology 2024Quote: ... and qPCR was carried out in 5 µL multiplexed reactions and 384-well format using the LightCycler 480 Instrument II (Roche). Final concentration of primers are 0.5 µM and probes are 0.25 µM ...
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bioRxiv - Molecular Biology 2024Quote: ... and 5 µl of diluted cDNA was used for each qPCR reaction carried out using KAPA SYBR FAST master mix (Roche). Relative expression levels for each gene were determined based on the 2−ΔΔCT method normalizing against 7SK RNA.
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bioRxiv - Cancer Biology 2024Quote: ... cells were co-transfected with 5 μg of Myc-tagged NAT10 and 5 μg of BRD4 short or long isoform using the Xtremegene 9 DNA transfection reagent (Roche). After overnight incubation ...
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bioRxiv - Microbiology 2024Quote: ... The bacterial pellets were then resuspended in 20 ml of lysis buffer pH 8.0 (50 mM NaH2PO4, 300 mM NaCl, 5 mM Imidazole) containing 1× cOmplete EDTA-free protease inhibitor cocktail (Roche, Germany) and 1 mg/ml lysozyme ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were subsequently rinsed and incubated in the dark with a NBT/BCIP solution (Nitroblue tetrazolium chloride/ 5-bromo-4chloro-3-indyl-phosphate; Roche) until the staining appeared (overnight or up to 48 h) ...
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bioRxiv - Molecular Biology 2024Quote: ... Adapters were ligated by adding 15 µL of the ligation mix (3 µL T4 Ligase Buffer 10X, 0.5 µL T4 ligase (5 U/µL, Roche, 10799009001), 0.25 µl of x-Gene Stubby Adapter 50 mM (IDT) ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfection of 106 cells in a 6-well plate was performed using 5 μl of transfection agent (X-tremeGENE HP Reagent, Roche), 10 μL of bacmid and 85 μL of SF900II-SFM medium (Gibco) ...
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bioRxiv - Bioengineering 2024Quote: ... the supernatant was discarded, and 5 mL of Hank’s Balanced Salt Solution (Ca-, Mg-) (gibco) buffer containing liberase (Roche, #05401119001) (50 mg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... followed by an 8-minute perfusion with myocyte digestion buffer containing 5 mg of liberase dispase high DH enzyme (Roche) per digestion ...
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bioRxiv - Cancer Biology 2024Quote: ... Fluorophores with the least autofluorescence on FFPE tissue were selected to minimize false positives: Cyanine 5 (Cy5) (DISCOVERY CY5 Kit, Cat#760238, Roche Diagnostics Corporation ...
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bioRxiv - Immunology 2024Quote: ... All lung lobes were cut into small pieces and digested in R5 media (RPMI 1640 supplemented with FCS (5 %) containing DNase I grade II (30 µg/ml) and Liberase TM (50 µg/ml, Roche) for 45 min at 37 °C with a magnetic stirrer.
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bioRxiv - Developmental Biology 2023Quote: ... with probe prepared by nick translation of a double-stranded PCR product bearing the bcd ORF using alkali-stable digoxigenin-11-2’-deoxyuridine-5’-triphosphate (Roche), visualizing with alkaline-phosphate coupled sheep anti-digoxigenin Fab fragments (Roche ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... Coralville, Iowa, USA) and 0.25 µl reverse primer (5 µM, IDT) and was analyzed on a LC480 instrument (Roche, Basel, Switzerland).
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bioRxiv - Cancer Biology 2021Quote: C2C12 cell lysate was generated using 5 million cells in RIPA lysis buffer with protease/phosphatase inhibitors (Roche #11836145001 and #04906837001), and 20μg of protein was loaded on a denaturing SDS page gel for detection of the VGLL2-NCOA2 fusion ...
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bioRxiv - Immunology 2020Quote: ... The proliferation rate of lymphocytes was measured using 5-Bromo-2-deoxyuridine (BrDU) assay as per the manufacturer instructions (Cell Proliferation ELISA BrDU, Roche, USA).