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Citations for Roche :
1151 - 1200 of 8815 citations for 6 Methyl 5 4 phenyl 1 3 thiazol 2 yl 2 trifluoromethyl nicotinic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 2 mM EDTA) supplemented with 1X cOmplete EDTA-free protease inhibitor cocktail (Roche), 2 mM MgCl2 ...
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bioRxiv - Biophysics 2024Quote: ... supplemented with 2 mg/ml iodoacetamide and complete Protease Inhibitor Cocktail Tablets (Roche). The extraction mixture was stirred at 4°C for 1Dhour ...
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bioRxiv - Cancer Biology 2022Quote: ... expanded to passage 4 and transfected with RCAS-PDGFB-HA or RCAS-shp53-RFP using a Fugene 6 Transfection kit (Roche, 11814443001). Cells were cultured with DMEM media (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... levodopa (Madopar®, Roche, Levodopa/carbidopa, ratio 4:1) was administered twice daily for 4-5 months at an individually-tailored dose designed to produce a full reversal of the parkinsonian condition (p.o ...
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bioRxiv - Biochemistry 2024Quote: ... was washed once with 80 mL of lysis buffer 1 (20 mM Tris-HCl, pH 8.0) containing 2 mL of EDTA-free protease inhibitor mixture (Roche Applied Science, Penzberg, Germany). Cells were disrupted by passing the cell suspension through a Constant Cell disruption system (TS benchtop ...
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bioRxiv - Cell Biology 2024Quote: Four mL of nuclear lysate from suspension culture of HeLa cells at protein concentration of 2.5 mg/mL were prepared in buffer (50 mM HEPES, pH 7.4, 150 mM NaCl, 2 mM MgCl2, 1 mM DTT, cOmplete (La Roche Ltd., Basel, Switzerland, 05056489001) and PhosStop RNase inhibitors (La Roche Ltd. ...
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bioRxiv - Cell Biology 2024Quote: Two mL of nuclear lysate from suspension culture of HeLa cells at protein concentration of 2.5 mg/mL were prepared in buffer (50 mM HEPES, pH 7.4, 150 mM NaCl, 2 mM MgCl2, 1 mM DTT, cOmplete (La Roche Ltd., Basel, Switzerland,05056489001) and PhosStop RNase inhibitors (La Roche Ltd. ...
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bioRxiv - Cell Biology 2024Quote: Four mL of nuclear lysate from suspension culture of HeLa cells at protein concentration 2.5 mg/mL were prepared in buffer (50 mM HEPES, pH 7.4, 150 mM NaCl, 2 mM MgCl2, 1 mM DTT, cOmplete (La Roche Ltd., Basel, Switzerland, 05056489001) and PhosStop RNase inhibitors (La Roche Ltd. ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 mM ethylenediaminetetraacetic acid) supplemented with protease and phosphatase inhibitors (Sigma and Roche). Proteins were immobilized on PVDF membranes (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... 0.25 mg/ml yeast RNA and 1 % blocking reagent for nucleic acids (Roche, Switzerland) was used ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Slides were rinsed in maleic acid buffer and blocked with 1% blocking reagent (Roche). Subsequently ...
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bioRxiv - Genomics 2022Quote: ... 5 µL of 20 mM siRNAs and 3 µL of X-tremeGENE HP DNA Transfection Reagent (Roche, #6366546001) were diluted in 200 µL Opti-MEM (Gibco ...
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bioRxiv - Genomics 2020Quote: ... The 2nd strand was synthesized using 2nd primer (5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNNNNNGTGGTATCAACGCAGAGTAC -3) with KAPA HiFi HS mix (KAPA Biosystems). The double stranded cDNAs were amplified using Illumina adapter-specific primers and LongAmp Taq DNA polymerase (NEB) ...
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bioRxiv - Cell Biology 2024Quote: ... 20 mM Tris pH 7.4, 0.5 mM EDTA, 5 mM KCl, 3 mM MgCl2, protease inhibitor, and phosphatase inhibitor cocktail from Roche). A 500 µl aliquot of the lysate was reserved for total liver proteome analysis ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were arrested at metaphase by incubating with colcemid (N-methyl-N-deacetyl-colchicine, Roche, 10295892001) for the last 14 hours before harvesting the cells ...
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bioRxiv - Genetics 2020Quote: ... These pieces were then added to 3-4 ml of digestion media containing 0.1 mg/ml Liberase (Roche, 501003280), 100 U/ml DNase I (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA (3–4 μg) was used to prepare cDNA with the Transcriptor First-Strand cDNA synthesis kit (Roche). qPCR was performed with TaqMan gene expression assay primers (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Myelin was washed by dilution in HBSS and centrifuged at 400 x g for 5 min at 4 °C before suspending in 3 mL ice cold 0.32 M sucrose solution containing protease inhibitor cocktail (Roche). Next ...
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bioRxiv - Bioengineering 2023Quote: ... 10-12 DRGs per donor were disintegrated into micro tissue units using 6 mL collagenase P (4 mg/mL, Roche, Mannheim, DE) in a 15 mL Falcon tube on an orbital shaker for 2 h at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... DNA (1 μg) was mixed with 3 μl Fugene6 (Roche, #11836145001) in 200 μl opti-MEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic acids were extracted using the High Pure Viral Nucleic Acid Kit (Roche) according to the manufacturer’s recommendations.
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was transferred to a blocking buffer (1 M maleic acid solution pH7.4, 1% nucleotide blocking reagent (Roche)) for 30 minutes and then incubated in antibody-solution (anti-dioxigenin-AP fragments in blocking buffer) ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% Zwittergent 3-12 (N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate) and protease inhibitor (Complete, Roche Applied Science)) for 2 h at 0°C (ref A) ...
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bioRxiv - Neuroscience 2022Quote: ... were transfected into HEK293 cells at a ratio of 3:3:1 (hGluN1/hGluN2A/EGFP) using X-tremegene HP (Roche) at a 1:100 ratio with optiMEM ...
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bioRxiv - Genomics 2020Quote: ... the tissues were cut into 1-2 mm2 pieces and put in 1mg/ml collagenase and dispase (C/D) solution (Roche Diagnostics GmbH Roche Applied Science, Mannheim, Germany) at 37°C for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Fractions were supplemented with SDS (to a final concentration of 1%) and then digested with proteinase K (Roche, final concentration of 2 µg/ml) for 45 min at 42 C ...
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bioRxiv - Biochemistry 2023Quote: ... and were resuspended in 30 mL lysis buffer 1 (50 mM sodium phosphate, 300 mM NaCl, pH 8) containing 2 mL of EDTA-free protease inhibitor mixture (Roche Applied Science, Penzberg, Germany). Cells were disrupted by passing the cell suspension three times through a Constant Cell disruption system (TS benchtop ...
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bioRxiv - Cell Biology 2024Quote: ... a final concentration of 1 ng/μl cDNA was mixed with 2 μl FastStart DNA Master SYBR Green I (Roche #03 003 230 001), 0.5 μM forward and reverse primer ...
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bioRxiv - Genetics 2024Quote: ... 30 mM Tris-HCl, 20 mM KCl, 2 mM MgCl2, 1 mM phenylmethylsulfonyl fluoride, 150 mM NaCl, cOmplete proteinase inhibitor [Roche Diagnostics, Indianapolis, IN, USA]), lysed by ultrasonic treatment and incubated with EZview anti-HA agarose beads (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Isolated hepatocytes were seeded at a density of 4-500,000 and 1,000,000 cells in rat tail collagen I (5 μg/cm2, Roche) pre-coated 6-well plates and T25 flasks ...
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bioRxiv - Cancer Biology 2024Quote: ... which then were transferred into 4-5 fold bigger volume of cOmpleteTM Protease Inhibitor Cocktail (Roche, Merck) in PBS and gently mixed for 30 minutes to aid HA dilution ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM PMSF) supplemented with 1x protease inhibitor cocktail (Roche cOmplete Protease Inhibitor Cocktail) and lysed using a Dounce homogenizer (20 strokes with loose plunger followed by 20 strokes tight plunger) ...
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bioRxiv - Cell Biology 2020Quote: ... the bile duct was cannulated and perfused with 2◻mL collagenase P (Roche Diagnostics ...
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bioRxiv - Molecular Biology 2021Quote: ... Briefly, cells were lysed in lysis buffer (2% SDS, 1X protease inhibitor cocktail (Roche), and 1 mM sodium azide in 1X TBS ...
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bioRxiv - Neuroscience 2020Quote: ... followed by reduction and alkylation in 10 mM Tris (2-carboxyethyl) phosphine hydrochloride (Roche) and 55 mM iodo-acetamide (Sigma-Aldrich) ...
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bioRxiv - Genomics 2020Quote: cDNA products were amplified with KAPA HiFi HotStart Uracil 2 x ReadyMix (Kapa Biosystems) and newly designed PCR primers that contain a deoxyuraciland one of which was biotinylated ...
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bioRxiv - Neuroscience 2020Quote: ... pH 9.5) was applied for 2 minutes before adding CDP-star (25 mM, Roche) for 5 minutes in the dark ...
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bioRxiv - Biophysics 2021Quote: ... which was supplemented with 2 x cOmplete™ EDTA-free protease inhibitor tablets (Roche), 15 μg/mL DNase I (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM EDTA) with protease inhibitor cocktail (04693116001, Roche Life Science, Indianapolis, IN, USA) and Phosphatase Inhibitor cocktail (P0044 ...
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bioRxiv - Immunology 2020Quote: ... Livers (minced) and pooled deciduae were incubated in 2 mg/mL collagenase D (Roche) at 37 °C for 30 – 40 minutes with agitation ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5 mM Tris(2-carboxyethyl) phosphine (TCEP) supplemented with a protease inhibitor cocktail (Roche,cOmplete Ultra EDTA-free ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were isolated by sequential digestion with pronase (2 mg/mL, PRON-RO, Roche) at 37 degrees ...
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bioRxiv - Cell Biology 2022Quote: ... and 2 mM NaF supplemented with complete mini protease inhibitor cocktail (Roche, Laval, QC)) ...
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bioRxiv - Systems Biology 2022Quote: ... 2% SDS) and in presence of 1x complete protease inhibitor cocktail (Roche, Basel, Switzerland). Samples were then sonicated using Bioruptor Pico (Diagenode ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were washed 2 times with 1x PBS with protease inhibitor cocktail (Roche, 11836170001). They were lysed on the plate with cold Farnham lysis buffer to ~10×10^6 cells /mL (5mM PIPES pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 infection was confirmed by the RT-PCR-COBAS 6800 System (Roche Molecular Systems ...
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bioRxiv - Physiology 2020Quote: ... The pellet was then suspended in DMEM containing 2 mg/mL collagenase/dispase (Roche), 0,147 μg/mL TLCK (Lonza ...
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bioRxiv - Microbiology 2021Quote: ... 2 mM EDTA and cOmplete ™ EDTA-free Protease Inhibitor Cocktail (Roche product 11836170001) for 30 min on ice ...
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bioRxiv - Biophysics 2020Quote: ... containing 0.5% (w/v) SDS and 2 μg of proteinase K (Roche, cat. #3115887). DNA fragments were extracted with phenol/chloroform and analyzed by electrophoresis in a non-denaturing 10% polyacrylamide gel ...
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bioRxiv - Microbiology 2020Quote: ... 10μL of 2× FastStart TaqMan® Probe Master Mix (Roche Life Science, Mannheim, Germany), 750 nM cruzi1 and cruzi2 primers and 250 nM cruzi3 probe (FAM/NFQ-MGB ...