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Citations for Roche :
951 - 1000 of 8815 citations for 6 Methyl 5 4 phenyl 1 3 thiazol 2 yl 2 trifluoromethyl nicotinic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: We amplified the cDNA using 2× Kapa HiFi HotStart ReadyMix (Roche), a high-fidelity polymerase enzyme ...
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bioRxiv - Cancer Biology 2024Quote: ... consisting of 2 μL 5X KAPA HiFi HotStart Buffer (Roche, KK2502), 0.048 μL of 10 mM dNTPs ...
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bioRxiv - Immunology 2023Quote: ... the media was supplemented with IL-2 [10 lU/mL] (Roche) and IL-15 [2 ng/ml] (BioLegend) ...
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Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: ... with 2 µL of the Fluorescein labeling mix (Roche, Basel, Switzerland), instead of the recommended 2 µL of each NTP ...
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bioRxiv - Molecular Biology 2023Quote: ... and 2 mM DTT) supplemented with complete protease Inhibitor cocktail (Roche) and 0.1 mg/ml cycloheximide or no cycloheximide ...
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bioRxiv - Biochemistry 2022Quote: ... and 2 mM ATP) using one Protease Inhibitor Cocktail Tablet (Roche) per 100 mL of the lysate ...
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bioRxiv - Immunology 2022Quote: Cells in cRPMI-10 with 20 U/ml IL-2 (Roche) were activated with Cell Stimulation Cocktail (Affymetrix eBioscience ...
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bioRxiv - Genomics 2022Quote: ... transposed fragments were amplified using 2×KAPA HiFi HotStart ReadyMix (Roche) and Nextera DNA CD Indexes (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... 2 mM β-mercaptoethanol) supplemented with protease inhibitor cocktail (#11873580001, Roche) and 200 unit/ml RNAsin (#N2515 ...
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bioRxiv - Molecular Biology 2023Quote: ... Then 2 µL of 20 mg/mL Proteinase K (#3115879001, Roche) was added and again incubated at 55°C for 2 h ...
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bioRxiv - Microbiology 2023Quote: ... 2% glycerol (v/v)] supplemented with complete protease inhibitor cocktail (Roche). Cell lysates were scraped into a pre-chilled micro-centrifuge tube and incubated with agitation for 30 min at 4°C followed by sonication at 40% intensity three times in 10 second intervals ...
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bioRxiv - Developmental Biology 2023Quote: ... and 2 ng/ml basic fibroblast growth factor (Roche Applied Science). Experiments were performed at passages 6-8.
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bioRxiv - Immunology 2023Quote: ... 2 mM sodium vanadate) supplemented with cOmplete protease inhibitor cocktail (Roche), 100 μM PMSF ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.1% Tween 2) followed by incubations in MABT blocking solution (Roche) and anti-DIG-AP Fab fragments (Roche) ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mg/ml lysozyme] with 1X protease inhibitor cocktail (Roche, USA) for 1 hour at 4 °C and subjected to sonication (30 sec pulse with 50 sec intervals for 10 mins at 40% power on ice ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 2× cOmplete Mini EDTA-free Protease Inhibitor Cocktail (Roche) for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated in DAPI (2 µg/ml, 10236276001, Roche Merck) in PBS (10 min) ...
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bioRxiv - Bioengineering 2024Quote: ... 2 U/mL bovine DNase I (Roche, Switzerland, Cat. No. 11284932001), 25 mM MgCl2 ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 μl FastStart Universal Probe Master 2× (Rox) Mix (Roche, Switzerland) and ddH2O up to 20 μl of total volume ...
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bioRxiv - Bioengineering 2024Quote: ... the samples were homogenized with 2 protease inhibitor cocktail tablets (Roche). The cells were lysed by sonication and centrifuged at 18000 rpm at 4 °C for 30 minutes to separate the insoluble and soluble fractions ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mM DTT and EDTA-free cOmplete protease inhibitor (Roche #11873580001), then lysed by probe sonication ...
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bioRxiv - Cell Biology 2020Quote: ... 3 % SDS and 5 mM EDTA) supplemented with 1x complete protease inhibitor cocktail (Roche, Switzerland) and 1 mM phenylmethylsulfonyl fluoride ...
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bioRxiv - Cell Biology 2020Quote: ... as well as AP substrate consisting of 5-bromo-3-chloro-indolyl phosphate (Roche Diagnostics) and 4-nitro blue tetrazolium chloride (Roche Diagnostics ...
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bioRxiv - Molecular Biology 2022Quote: To determine the repM transcription start site a 2nd Generation 5’/3’ RACE Kit (Roche) was used according to the manufacturer’s instructions with some modifications ...
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bioRxiv - Developmental Biology 2023Quote: ... Primer2: 5’-CAAGCAGAAGACGGCATACGA*G-3’) and 15µl 2x Kapa HiFi HotStart Ready Mix (Kapa Biosystems), amplification was performed for 45 s at 98°C ...
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bioRxiv - Plant Biology 2024Quote: ... 15 mM MgCl2, 1 mM EDTA, 10 % glycerol, 1 % Triton X-100, 10 mM β-mercaptoethanol, 2 mM PMSF and Roche cOmplete Protease Inhibitor Cocktail)) were added in the ground leaves and incubated for 30 min at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.3% 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS)) supplemented with 1X protease inhibitor cocktail (Complete; Roche), 0.5 mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Immunology 2021Quote: ... washed in FACS buffer (4% FBS, 3 mM EDTA, and 40 μg/ml DNAse I (Roche)) and incubated with combinations of antibodies to CD45 (30-F11) ...
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bioRxiv - Genetics 2021Quote: ... followed by addition of 5 µl of 4 µg/mL DNase-free RNase (Roche). Samples were loaded onto 96-well Qiacube-HT® columns and DNA was purified using a Blood & Tissue kit (Qiagen) ...
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bioRxiv - Neuroscience 2021Quote: ... Glucose plasma concentration was measured with glucose oxidase method using 2’2-azino-bis (3-ethylbenzothialozine-6-sulfonate) (ABTS) (#10102946001, Roche, Germany) as substrate ...
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bioRxiv - Microbiology 2024Quote: ... spleens were collected from naïve C57BL/6 (CD45.1+) and a single cell suspension was obtained by incubation with Liberase Blendzyme 3 (Roche, Bradford, CT) and DNase (Sigma ...
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bioRxiv - Pathology 2023Quote: ... in HEK 293T cells (3×105 cells per well in 6-well plates) using X-treme gene transfection reagent (Roche). Pseudotyping was achieved by co-transfecting pHEF-VSVg (400 ng/well) ...
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bioRxiv - Biochemistry 2022Quote: (His)6-GST-SNX15 MIT was bound to cOmplete His-Tag purification beads (5 mL, Roche, Germany, 2h) and washed with 2 L wash buffer ...
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bioRxiv - Genetics 2024Quote: ... 1×EDTA (Ethylene Diamine Tetraacetic Acid)-free protease inhibitor cocktail (Roche 04693132001). The larvae were then crosslinked by adding formaldehyde to a 1.8% concentration and incubating for 5mins at RT on a rotator ...
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bioRxiv - Plant Biology 2021Quote: ... 90 mM KCl, 2 mM EDTA, 0.5 mM spermidine, 0.2 mM spermine, 0.5 mM EGTA, 1× Roche Complete protease inhibitor cocktail). Nuclei were isolated by sucrose density gradient centrifugation ...
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bioRxiv - Biochemistry 2020Quote: ... mouse Hepa 1-6 and rat Fao hepatoma cells at 75% confluency were incubated with a mixture of oleate and palmitate (ratio 2:1, total concentration 1.2 mM) coupled to FA-free Bovine Serum Albumin (BSA) (Roche Applied Sciences). All fatty acid stocks were initially reconstituted in absolute ethanol ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated for 2 hours at room temperature with an anti-FL antibody conjugated with HRP (1/500, Roche Diagnostics) followed by reaction with TSA biotin reagents (Perkin Elmer ...
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bioRxiv - Developmental Biology 2020Quote: ... pH 7.4, 150 mM NaCl, 1% NP-40, 2 mM EGTA, and complete protease and phosphatase inhibitors from Roche, Basel, Switzerland). Cell lysate was cleared by centrifugation ...
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bioRxiv - Cell Biology 2021Quote: ... 1 M KCl, 2 mM EDTA, 1 mM DTT, 0.01 U/ml benzonase and 1x EDTA-containing protease inhibitor cocktail [Roche Applied Sciences]). The lysate was cleared by centrifugation at 18,000 x g for 45 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... plus phosphatase and protease inhibitors (2 mM NaVO4, 10 mM NaF, and 1 mM PMSF) and Complete protease inhibitor cocktail (Roche, CA). For tissue extracts ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue sections were cut into small pieces (1-2 mm) and incubated with 0.25 mg/ml Liberase TL (Roche, Cat# 05401020001) at 37°C for 40 minutes ...
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bioRxiv - Physiology 2022Quote: ... 2 mM EDTA, 20 mM Tris pH 8, 150 mM NaCl, 1 mM PMSF, 1X cOmplete™ Protease Inhibitor Cocktail, Roche) was added to the fragmented chromatin ...
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bioRxiv - Genomics 2023Quote: hTERT RPE-1 cells at 70% confluence were harvested by trypsinization after 2-hour treatment with colcemid (100 ng/ml, Roche 10295892001) to arrest cells in mitosis ...
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bioRxiv - Cell Biology 2024Quote: ... and the adipocytes were lysed using PBS containing 2% SDS and protease inhibitor (1 tablet per 10 mL; cOmplete Mini, Roche Diagnotics). Cell lysates were centrifuged at 14000 rpm for 10 minutes at 4 0C and total protein contents were measured by Bradford protein assay ...
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bioRxiv - Cell Biology 2024Quote: ... and the adipocytes were lysed using PBS containing 2 % SDS and protease inhibitor (1 tablet per 10 mL; cOmplete Mini, Roche Diagnotics). Secretion samples were centrifuged for 5 minutes at 2000 rpm and 4 0C ...
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bioRxiv - Neuroscience 2023Quote: ... followed incubation with blocking solution for 2 hours before incubation with alkaline phosphatase (AP)-conjugated anti-DIG Fab fragments antibody (1:1000, Roche, #11093274910) overnight at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... Total proteins from mixed samples (14N-flg22/15N-mock and 14N-mock/15N-flg22) were extracted with IP buffer 2 with 1× Protease Inhibitor (Pierce) and phosphatase inhibitor (Roche, PhosSTOP), and centrifuged for 1700g for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... and proteins were extracted in ice-cold lysis buffer (150 mM NaCl, 2 mM EDTA, 40 mM HEPES, and 1% Triton X-100) supplemented with phosphatase (Roche #04906837001) and protease inhibitor cocktail ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM DTT, 2 μM MG-132 proteasome inhibitor (#S2619, SelleckChem) and cOmplete® ULTRA EDTA-free inhibitor cocktail (#5892953001, Roche). Approximately 0.1 ml of 0.6 mm glass beads were added and thoroughly mixed ...
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bioRxiv - Immunology 2024Quote: ... The tissue was weighed and digested with 10μL mg−1 DISC lysis buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% TritonX-100, 10% glycerol, H2O, protease inhibitor cocktail tablet: Roche, Basel, Switzerland). The tissue was lysed with TissueLyser85300 (Qiagen ...