Labshake search
Citations for Roche :
1101 - 1150 of 2327 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2019Quote: ... we transiently transfected 293T cells using ExtremeGENE 9 according to the manufacturer’s protocol (Roche). After 48 hours ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... The plasmid constructs were transfected into HEK293 cells by using X-tremeGENE HP (Roche), according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: Cell pellets were lysed in RIPA buffer (Applygen Technologies) containing phosphatase inhibitor (Roche Diagnostics) and protease inhibitor (Fermentas) ...
-
bioRxiv - Cell Biology 2020Quote: ... Cell pellets were resuspended in RIPA buffer supplemented with cOmplete Protease Inhibitor Cocktail (Roche) and PhosSTOP (Roche ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cell lysates (100–200 μg) were then immunoprecipitated with GFP antibody (Roche Applied Science). Ubiquitination was detected as described previously (38).
-
bioRxiv - Cancer Biology 2020Quote: ... cells were treated with 1 μg/mL DAPI (10236276001, Roche Diagnostics GmbH, Mannheim, Germany). Stained sections were detected by a laser-scanning confocal microscope (Leica TCS SP8 ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were pelleted and resuspended in PBS + Complete Mini EDTA-free protease inhibitor (Roche). Cells were lysed by sonication using a W-385 Sonicator Ultrasonic Processor (Heat Systems - Ultrasonics ...
-
bioRxiv - Biochemistry 2021Quote: ... Viable cell number was assessed 48 hr later using the WST-1 reagent (Roche), according to manufacturer’s specifications ...
-
bioRxiv - Developmental Biology 2020Quote: ... DNA from lysed cells was removed using 5 μl RNAse-free DNAse I (Roche) for every 1 ml dissociate and incubated at 37°C for 5 minutes ...
-
bioRxiv - Cancer Biology 2021Quote: ... Histone-associated DNA-fragment levels were analyzed using the Cell Death Detection ELISAPLUS (Roche; Cat ...
-
bioRxiv - Cancer Biology 2020Quote: ... which quantifies mitochondrial metabolic activity of viable cells per manufacturer’s instructions (Roche, Indianapolis, IN). Cells were cultured in 96-well microplates in a concentration of 5×104 cells/mL (in RPMI with 10% FBS ...
-
bioRxiv - Genomics 2021Quote: ... Cells were then washed three times in ice cold PBS containing protease inhibitors (Roche), flash frozen ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were washed twice with PBS and incubated with 2.4 U/ml dispase (Roche) for 1h at 37°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... cell lysates were prepared using the IP lysis buffer (0.5% NP40 (Roche, Basel, Switzerland), 1x cOmplete protease inhibitor (Roche ...
-
bioRxiv - Molecular Biology 2021Quote: ... Flow cells were functionalized by incubating with 0.1 mg/mL anti-digoxigenin antibody (Roche) and 3 µm polystyrene beads (Polysciences ...
-
bioRxiv - Genomics 2021Quote: ... Cells were washed with PBS supplemented with 1X of Complete protease inhibitor cocktail (Roche), pelleted and frozen ...
-
bioRxiv - Genetics 2020Quote: ... fly head or cell lysates were lysed in RIPA buffer with protease inhibitors (Roche) and passed through a 27-gauge needle to shear DNA ...
-
bioRxiv - Neuroscience 2021Quote: ... cells were lysed in RIPA lysis buffer (iNtRON) containing complete protease inhibitor cocktail (Roche), followed by SDS-PAGE and incubation with primary and secondary antibodies ...
-
bioRxiv - Neuroscience 2022Quote: ... fragmented nucleosomes formation was quantified using the Cell Death ELISAPLUS assay (Roche Applied Science), as previously published [38] ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were transfected with plasmid DNA using X-tremeGENE HP DNA transfection reagent (Roche) as per the manufacturer’s protocol using a total vector amount of 2 µg (6-well ...
-
bioRxiv - Cancer Biology 2022Quote: ... RNA was extracted from WILD-seq transduced cells (High Pure RNA isolation kit, Roche) and reverse transcribed using the Superscript IV reverse transcription kit (Invitrogen ...
-
bioRxiv - Cancer Biology 2022Quote: ... red blood cell lysis was performed according to the manufacturer’s instructions (ROCHE, Cat# 11814389001). Cell number and viability were assessed on a Luna-FLTM Dual Fluorescence Cell counter (Logos Biosystems Inc. ...
-
bioRxiv - Cell Biology 2022Quote: Cell lysates were prepared by lysing in RIPA buffer with protease inhibitor cocktail (Roche) and protein concentrations were normalized using a BCA assay (Thermo Scientific) ...
-
bioRxiv - Cell Biology 2022Quote: ... Cell lysates were diluted with 500uL of RIPA buffer with protease inhibitor cocktail (Roche) and subjected to immunoprecipitation with G1 anti-DYKDDDDK affinity resin overnight at 4°C ...
-
bioRxiv - Cell Biology 2022Quote: The TUNEL assay was performed using the In Situ Cell Death Detection Kit (Roche) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: Total RNA was isolated from cells using the High Pure RNA isolation kit (Roche) on a MagnaPure Machine and used for qRT-PCR ...
-
bioRxiv - Microbiology 2022Quote: Cells were lysed using M-PER (ThermoScientific) supplemented with 1x protease inhibitor tablet (Roche) according to manufacturer instructions ...
-
bioRxiv - Microbiology 2022Quote: ... Cell pellets were resuspended in 1.0 ml IP buffer containing protease inhibitor cocktail (Roche), cells were lysed and insoluble matter was removed by centrifugation at 13000 rpm for 10 min at 4°C ...
-
bioRxiv - Physiology 2022Quote: ... TUNEL staining was performed with the In Situ Cell Death Detection Kit (fluorescein) (Roche) in a humidified chamber at 37 °C for 1 h.
-
bioRxiv - Biochemistry 2022Quote: ... Cells were lysed in hypotonic lysis buffer (10 mM Tris/HCl pH 8, Roche protease inhibitors ...
-
bioRxiv - Cell Biology 2022Quote: ... HepG2 cells were transfected with PX459-p62 with X-tremeGENE HP transfection reagent (Roche). Twenty-four h after transfection ...
-
bioRxiv - Immunology 2022Quote: Cells were lysed with RIPA buffer containing EDTA-free cOmplete protease inhibitor cocktail (Roche) and Halt phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The plasmids were transfected into HEK293T cells using X-tremeGENE 9 (Roche # XTG9-RO) as per the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were harvested in 1X PBS supplemented with protease inhibitors (cOmplete cocktail, 11873580001, Roche). Cells were centrifuged at 3,000 rpm for 10 minutes at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... cell pellets were resuspended in RIPA buffer supplemented with cOmplete Protease Inhibitor Cocktail (Roche), phenymethylsulphonyl fluoride (PMSF ...
-
bioRxiv - Immunology 2023Quote: ... cells were harvested and resuspended with permeabilization buffer containing protease inhibitor cocktail (11836153001, Roche) and incubated for 10 min on ice with constant mixing ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were lysed in High Salt RIPA buffer with protease (cOmplete EDTA-Free, Roche), phosphatase inhibitors (PhosSTOP ...
-
bioRxiv - Cell Biology 2023Quote: Cultured cells were lysed in RIPA buffer supplemented with protease inhibitors (Roche, NJ, USA). Then ...
-
bioRxiv - Synthetic Biology 2023Quote: ... cell pellets were treated with 1 mL of 2 mg/mL lysozyme (Roche, Switzerland) and incubated at 30 °C for 10 min ...
-
bioRxiv - Neuroscience 2023Quote: ... 0,5×106 cells were lysed in RIPA buffer containing cOmplete Protease Inhibitor cocktail (Roche). Protein extraction and Western blotting is performed as previously described [109] ...
-
bioRxiv - Immunology 2023Quote: Cell lysates were prepared in RIPA buffer supplemented with complete protease-inhibitor cocktail (Roche) and mixed in equal amounts with Laemmli buffer (Roth ...
-
bioRxiv - Microbiology 2023Quote: ... 20 µl TUNEL reaction mixture (In situ Cell Death Detection Kit, TMR-red, Roche) was then added to each coverslip and the reaction incubated in the dark for 1 hour at 37°C ...
-
bioRxiv - Immunology 2023Quote: ... cells were lysed with 1x RIPA buffer supplemented with 1x cOmplete proteinase inhibitor (Roche) for 30 min on ice ...
-
bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from fresh cells using high pure RNA isolation kit (Roche) as per manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were supplemented with 0.5% Triton X-100 and cOmpleteTM Protease Inhibitor Cocktail (Roche), incubated on ice for another 15 min and spun down at 1000 g for 5 min at 4°C ...
-
bioRxiv - Bioengineering 2023Quote: ... HEK293T cells were transfected using X-tremeGENE™ HP DNA Transfection Reagent (Roche, 6366244001) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were lysed in 1 ml of lysis buffer with Complete protease inhibitor (Roche). Lysates were passed through a 27 G needle ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were evaluated for senescence by p16 immunocytochemistry as described below (Roche, CINtec 06695248001). Cells were imaged using a Leica DMI-6000 spinning disc confocal microscope running Velocity imaging software ...
-
bioRxiv - Cell Biology 2023Quote: ... productively edited cells were selected with 0.3 mg mL-1 hygromycin B (Roche, 10843555001). Homozygously edited clones were identified using the same genotyping strategy as described above and cells which efficiently depleted WAPL were identified using confocal microscopy and Western blotting ...
-
bioRxiv - Cell Biology 2023Quote: Mouse wound beds were dissected and digested into single cells using Liberase TM (Roche) and cells were suspended in fluorescence-activated single cell sorting (FACS ...