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Citations for Roche :
1101 - 1150 of 8226 citations for 6 Chloro 2 3 4 9 tetrahydro 1H pyrido 3 4 b indol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cells were lipofected with pLacO using X-tremeGENE 9 DNA Transfection Reagent (Roche). The plasmid:transfection reagent ratio (w:v ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with X-tremeGENE 9 DNA Transfection Reagent (Roche, Basel, Switzerland) following manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... and lentiviral envelope vector CMV VSV-G using XtremeGene 9 transfection reagent (Roche). Viral particles were collected 48h after transfection and passed through a 0.45 μm filter to eliminate cells and cell debris ...
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bioRxiv - Molecular Biology 2020Quote: Cryosections (10μM) of ventricular tissue were fixed in 4% paraformaldehyde and stained with In-situ Cell Death Detection kit (Roche). Sections were imaged on a laser-scanning confocal microscope (LSM 510 ...
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bioRxiv - Genetics 2021Quote: ... Then they were incubated overnight at 4°C with primary antibodies diluted in a dilution buffer (0.5% blocking reagent (Roche), 2% fetal bovine serum ...
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bioRxiv - Developmental Biology 2020Quote: ... the embryos were washed and equilibrated in NTMT buffer followed by coloration with 4-nitro blue tetrazolium (NBT, Roche) and 5-Bromo-4chloro-3-indolyl-phosphate ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatants from each buffer were dialyzed in 25 mM Tris-HCl and 5 mM EDTA pH 8.0 overnight at 4°C and subsequently digested with 200 mg/ml pronase (Roche). Peptides were precipitated with 5% trichloroacetic acid (TCA ...
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bioRxiv - Cell Biology 2020Quote: ... 4 were purified according to the instructions of the manufacturer using the High Pure PCR Template Preparation Kit (Roche) followed by RNAse A digest and a final purification with the High Pure PCR Product Purification Kit (Roche).
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bioRxiv - Plant Biology 2021Quote: ... qPCR reactions were performed in 10 μl reactions containing 4 μl of LightCycler 480 SYBR Green I Master (Roche), 4 μl of PCR-grade water (Roche) ...
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bioRxiv - Neuroscience 2020Quote: ... The PBS was aspirated and 1 ml of homogenization buffer (320 mM sucrose, 4 mM HEPES NaOH, pH 7.4) supplemented with cOmplete EDTA-free protease inhibitor (Roche) and 1 mM ATP NaOH ...
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bioRxiv - Neuroscience 2019Quote: ... the pellet was re-suspended in SDS lysis buffer (137 mM Tris-HCl pH 6.8, 4% SDS, 20% Glycerol, Proteinase inhibitor, Roche), sonicated and quantified using the Pierce BCA Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... some TK rats (n=11) were orally administered 4 mg of valganciclovir (val) to reduce neurogenesis (Hoffman La-Roche; delivered in 0.5 g peanut butter + chow pellets ...
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bioRxiv - Molecular Biology 2019Quote: Cells were collected after two cold PBS washes by scraping in 2X SDS Lysis Buffer (4% SDS, 20% Glycerol, 120mM Tris-Cl pH 6.8, 1x protease (Roche) and phosphatase inhibitors (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2019Quote: ... cell pellets were lysed in 4% SDS/100 mM Tris HCl pH 7.4 supplemented with cOmplete protease inhibitor cocktail (Roche), and sonicated at 4 °C using a Diagenode Bioruptor ...
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bioRxiv - Developmental Biology 2021Quote: ... WB following 4-20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) employed HRP-conjugated anti-HA antibody (Roche). Quantification was performed by Image J software.
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bioRxiv - Genomics 2021Quote: ... These cells were re-suspended in lysis buffer (4% SDS, 50 mM Tris-HCl [pH 8.0], 10 mM EDTA, 100 mM NaCl) with Protease inhibitor (Roche) and RNase inhibitor ...
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bioRxiv - Evolutionary Biology 2020Quote: ... tissues were incubated at 4°C in a solution of 0.05% alkaline phosphatase-conjugated DIG antibodies (Roche cat #11093274910) overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... on a rotating wheel at 4 °C and all buffers were supplemented with protease inhibitor cocktail (#11873580 001, Roche). Prior to elution ...
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bioRxiv - Developmental Biology 2021Quote: ... WB following 4-20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) employed HRP-conjugated anti-HA antibody (Roche). Quantification was performed by Image J software.
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bioRxiv - Molecular Biology 2020Quote: ... the subventricular zone of 4 mice from each genotype was micro-dissected and tissue digested using Liberase DH (Roche) and DNAse I (250U/ml ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes were incubated overnight at 4° C on a rotator with primary antibody in Western Blocking Reagent (Roche), if not stated otherwise ...
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bioRxiv - Neuroscience 2022Quote: ... the supernatant was incubated at 4°C overnight with 20 μl anti-HA antibody coated beads (Roche, Rotkreuz, Switzerland) at RT ...
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bioRxiv - Neuroscience 2022Quote: ... Cultured cells/tissues were lysed with 4% SDS in TEA buffer (50 mM Triethanolamine, 150 mM NaCl, pH=7.3) supplemented with protease inhibitor cocktail (Roche). Total 500μg protein was treated with 10mM TCEP for 30min and then incubated with 25mM NEM for 2 h at room temperature to block unmodified cysteine residues ...
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bioRxiv - Molecular Biology 2022Quote: ... the isolated skin was incubated overnight at 4 °C in a tube containing 10 mL of 1x Dispase (Roche) and 2x Pen/Strep (Sigma ...
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bioRxiv - Immunology 2022Quote: ... was added and cells were centrifuged at 350g for 7 min at 4°C and resuspended in PBS with 0.5% BSA (Roche) and 1% FBS (Sorting Buffer) ...
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bioRxiv - Biophysics 2022Quote: ... The transcription mixture was incubated at 37 °C for 4 h and treated with 50U/mL DNAse I (Roche) at 37 °C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Precipitants were washed with acetone and dissolved in SDS-PAGE sample buffer containing 4 mM Pefabloc SC (11429876001, Roche). Equivalent protein amounts were subjected to immunoblotting analysis.
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bioRxiv - Cancer Biology 2023Quote: ... for 8 min followed by post-coloration using Bluing reagent for 4 min at RT (Roche Diagnostic, Meylan, France). The slides were then dehydrated (ethanol and xylene ...
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bioRxiv - Immunology 2023Quote: ... The epidermis and dermis layers were separated after incubation at 4°C for 16h in 0.2 mg/mL dispase II (Roche). Epidermal layers were fixed in 4% Para-formaldehyde for 20 min at room temperature ...
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bioRxiv - Immunology 2023Quote: Skin samples collected from the ventral neck area of 4-5 mice were minced in Hank’s balanced solution (HBSS) and incubated at 4°C overnight with 1.5mg/ml dispase II (Roche; 25766800). The next day ...
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bioRxiv - Plant Biology 2024Quote: ... the lysate was clarified by centrifugation at 12,000 x g and 4°C for 15 min and then filtered using a 0.45-µm syringe filter (Roche). The filtered lysate was passed over a gravity-flow column packed with 0.5 ml amylose resin (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... If the cells were fixed with 4% (v/v) paraformaldehyde solution and 0.1% (v/v) glutaraldehyde in vPBS containing EDTA-free protease inhibitor (Roche), the coverslips were directly mounted on glass slides after cell attachment ...
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bioRxiv - Molecular Biology 2024Quote: ... The remaining supernatant was diluted in 1 mL pre-warmed capturing buffer (0.5 M LiCl, 4 M urea, 100U Ribolock inhibitor, Thermo, 1x protease/phosphatase inhibitor, Roche) and 10 µL from an equimolar stock (10 µM ...
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bioRxiv - Neuroscience 2024Quote: The procedure involves decapitation of adult flies (sieving, ∼6,000 heads), pulverization, homogenization (320 mM sucrose, 4 mM HEPES, protease inhibitors (complete, 11873580001, Roche)) and differential centrifugation (from low speed to higher speed ...
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bioRxiv - Plant Biology 2019Quote: ... one with the Illumina Genomic DNA Sample Prep kit and one with the Kapa Hyper kit (Kapa Biosystem, Roche). Three mate-pair libraries were constructed using Illumina Nextera Mate Pair Library Prep kit with insert sizes ranging between 2-4 kb ...
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bioRxiv - Genetics 2020Quote: ... then resuspended in 600 µl of ChIP lysis buffer (50mM HEPES, pH 7.5, 140mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, one tablet Roche cOmplete mini EDTA-free Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM benzamidine) supplemented with 0.1 mg/mL lysozyme and one Complete EDTA-free protease inhibitor tablet (Roche) per 100 mL ...
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bioRxiv - Genomics 2019Quote: ... were lysed in 100 ul Buffer-B (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1%SDS, 1x protease inhibitors -Roche) and sonicated in a microtube (Covaris ...
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bioRxiv - Immunology 2023Quote: ... Aortas were manually homogenized and digested for 20 minutes at 37°C in RPMI with 1 mg/mL of collagenase A and collagenase B and 0.1 mg/mL DNAse I (Roche). Kidneys were homogenized using the Miltenyi gentleMACS Dissociator and digested for 20 minutes at 37°C in RPMI with 2 mg/mL collagenase D (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... 1% sodium deoxycholate, 50 mM NaF, 2 mM EDTA, 2 mM DTT, 0.2 mM Na orthovanadate, 1 X Roche protease inhibitor #11836170001). Lysates were sonicated and centrifuged to remove debris ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were lysed in 50 μl of Lysis Buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40, 1x Roche Complete protease inhibitors cocktail) by pipetting up and down ...
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bioRxiv - Developmental Biology 2021Quote: ... The DIG-labeled 3’UTR of Emi2 and cyclin B1 RNA probes were synthesized with a DIG-RNA-labeling kit (Roche Molecular Biochemicals). Two μg of probes was incubated with purified Flag-tagged proteins for 15 min ...
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bioRxiv - Cancer Biology 2020Quote: ... N3500], 50 mM of Tris [pH 7.5], 150 mM of NaCl, 3 mM of MgCl2, 1X protease inhibitors [Roche, Mannheim, Germany; 05892953001]). Protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: Total protein from cells or mouse tissues (n=3 per genotype) were extracted using the M-PER protein lysis buffer (ThermoScientific, Beverly, MA) containing protease inhibitors (Roche, Indianapolis, IN). Approximately 25 μg of total protein was electrophoresed on 4-12% SDS-PAGE gels and transferred to PVDF membranes ...
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bioRxiv - Physiology 2022Quote: ... n=3/genotype) were used for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) to visualize cell apoptosis/death (Roche, Basel, Switzerland). Sections were counterstained with DAPI and visualized using epifluorescence (Nikon Eclipse Ni E800) ...
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bioRxiv - Microbiology 2022Quote: ... 45 cycles of 95°C for 3 s and 60°C for 30 s using the LightCycler 96 system (Roche, Basel, Switzerland) or the StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genomics 2022Quote: ... Empty/filled PCRs (combining 5′ and 3′ flanking primers) and full-length PCRs (using junction-spanning primers) were performed using an Expand Long Range dNTPack (Roche, Cat#: 4829034001). Reaction mixes contained 5μL 5× Expand Long Range Buffer with 12.5mM MgCl2 ...
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bioRxiv - Cancer Biology 2019Quote: ... MA; N3500], 50mM of Tris [pH 7.5], 150 mM of NaCl, 3 mM of MgCl2, 1X protease inhibitors [Roche, Mannheim, Germany; 05892953001]). Protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Membranes were blocked for 1 h with 3% (w/v) non-fat milk powder in PBS/0.1% (v/v) Tween (PBST) (CALR, 10% Roche WBR in PBST) and incubated with primary antibody (Supplementary Table III ...
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bioRxiv - Genetics 2021Quote: ... The T7 promoter was added to the 5’ and 3’ ends to synthesize the sense and anti-sense probes using the DIG RNA Labeling Kit (Roche, Cat# 11175025910). In situ hybridization was performed as previously described [65,66] with minor modifications ...