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Citations for Roche :
1101 - 1150 of 8057 citations for 6 Chloro 1 2 dihydro 3H indazol 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: Batches of ten 2 day-old adult flies were crushed in 100 µl of lysis buffer (0.3% SDS in PBS plus one EDTA-free cOmplete™ Protease Inhibitor Cocktail tablet, Roche), incubated for 15 min and centrifuged at 15,000 gmax for 10 min (all manipulations at room temperature) ...
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bioRxiv - Genetics 2020Quote: ... One short-insert WGS library was constructed using the Kapa Hyper Prep Kit (KR0961) from Kapa Biosystems (Woburn, MA, USA) with 150 ng of sheared DNA as input following standard manufacturer’s protocol with 10 cycles of PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... Only one sample was obtained per patient: one portion of the sample was extracted using the High Pure Viral RNA Kit (Roche), and another portion of the same sample was extracted using the acid pH method ...
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bioRxiv - Microbiology 2021Quote: ... before being resuspended in 1 mL of lysis buffer (100 mM NaCl, 5% glycerol, 10 mM Tris, pH 8; one cOmplete Mini EDTA-free protease inhibitor pellet (Roche) per 15 mL) ...
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bioRxiv - Physiology 2022Quote: ... Fasting blood glucose was assessed by collecting blood from mice via tail puncture and using the one-touch Accu-Chek Aviva glucometer (Roche). Afterwards ...
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bioRxiv - Microbiology 2024Quote: ... RT-qPCR was performed using the Luna Universal One-Step RT-qPCR Kit (Biolaps) according to the manufacturer protocol on a LightCycler real-time PCR thermocycler (Roche). The analysis of the infection rate based on PCR data was done with the binGroup package [42] in R software.
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bioRxiv - Neuroscience 2023Quote: ... with one modification to Homogenization Buffer preparation required in which cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche, Basel, Switzerland) and Phosphatase Inhibitor Cocktail 2 (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... Pellets were resuspended in 7 ml of buffer A supplemented with the addition of 200 μl of protease inhibitor cocktail (one tablet (Roche), dissolved in 1 ml buffer A) ...
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bioRxiv - Developmental Biology 2023Quote: ... 2% sheep serum in PBST) for one hour and overnight at 4 °C with alkaline phosphatase-conjugated anti-DIG antibodies (Roche) diluted at 1/4000 in the blocking solution ...
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bioRxiv - Biochemistry 2023Quote: ... and the pellet was resuspended in 5 volumes of the lysis buffer (buffer A including one EDTA-free protease inhibitor tablet (Roche); buffer A ...
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bioRxiv - Systems Biology 2023Quote: ... Coverslips were washed with PBS three times for five minutes and blocked in a humid chamber for one hour at room temperature in blocking solution (1x Western Blocking reagent [Roche, 11921673001] diluted in PBS and supplemented with 0.3% Triton X-100) ...
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bioRxiv - Microbiology 2023Quote: ... To prevent adherence of cells to one another by DNA leakage the suspension of cells was incubated with 600 Units of DNAse I (Roche) (30 mins ...
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bioRxiv - Microbiology 2023Quote: ... Cells were grown under induction for one generation and homogenised using bead beater (MPBio) in lysis buffer with protease inhibitor cocktail (Roche): 12 cycles of 7 m/sec ...
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bioRxiv - Neuroscience 2024Quote: ... sciatic nerves (4 sciatic nerves were pooled for one preparation in order to generate enough material for transcriptomic analysis) and brains were enzymatically digested with Liberase DL (Roche) at 143μg.mL-1 and DNAse I (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... 2.5 mL of lysis buffer (50 mM MES, pH 6.5 at 4 °C, plus one cOmplete™ EDTA-free protease inhibitor cocktail tablet (Roche) per 50 mL ...
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bioRxiv - Molecular Biology 2021Quote: ... and 20 mM HEPES) supplied with 30 U/ml of human recombinant IL-2 (rIL-2, Roche). Four domains of SUPT16H ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed with 2% paraformaldehyde/ 2% sucrose for 20 min and stained with DAPI (#10236276001, Roche) to visualize anaphases ...
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bioRxiv - Cell Biology 2021Quote: ... cells were resuspended in complete media plus 10 IU/mL recombinant human IL-2 (rHIL-2, Roche), and seeded in fresh media at 0.5×106 cells/mL every 48 hours ...
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bioRxiv - Microbiology 2020Quote: ... the recombinant RBD007 plasmid was transfected using FuGENE 6 transfection reagents (Roche Applied Science, Indianapolis, IN) into CHO-K1 cells precultured in F-12K medium (American Type Culture Collection ...
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bioRxiv - Immunology 2021Quote: ... Th17-cell differentiation was induced using a cytokine cocktail of IL-6 (20 ng/mL; Roche), IL-1β (10 ng/mL ...
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bioRxiv - Genetics 2019Quote: ... The libraries were PCR amplified with KAPA HiFi for 4-6 cycles (KAPA Biosystems, Boston, MA). The final libraries were purified with two 0.7x AMPureXP bead cleanups ...
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bioRxiv - Cell Biology 2020Quote: ... Gag/Pol and VSV-G using Fugene-6 transfection reagent as per the manufacturer’s instructions (Roche) for 8 hours ...
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bioRxiv - Developmental Biology 2020Quote: Separate PLAT-E cultures were transfected with 9µg of each pMX plasmid using FuGENE 6 (Roche) for retroviral production ...
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bioRxiv - Immunology 2021Quote: ... in the absence (Th0) or presence (Th17) of IL-6 (20ng/ml, Roche, Cat# 11138600 001); IL-1β (10ng/ml ...
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bioRxiv - Immunology 2021Quote: ... Th17 cell polarization was induced using a cytokine cocktail of IL-6 (20 ng/mL; Roche), IL-1β (10 ng/mL ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots (6 μL) of tg7-brain homogenates were supplemented with a mixture of protease inhibitors (Roche), mixed with 5% glucose (94 μL ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Wings were stained at room temperature for 4-6 h in BM Purple (Roche Applied Science).
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bioRxiv - Neuroscience 2023Quote: ... Protein lysates were prepared from zebrafish larvae (6 dpf) in RIPA buffer containing protease inhibitors (Roche), using a manual dounce homogenizer ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 tetrazolium]-bis(4-methoxy-6-nitro)-benzene sulfonic acid hydrate (XTT)-based colorimetric assay (Roche) according to manufacturer-recommended protocols ...
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bioRxiv - Microbiology 2019Quote: Mature cysts were washed in PBS and suspended in lysis buffer (10 mM HEPES, 25 mM KCl, 1 mM CaCl2, 10 mM MgCl2, 2% CHAPS, and 1X Roche protease inhibitor) (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... endogenous peroxidase activity was blocked by adding H2O2 and incubating for 10 minutes followed by 2 more washes with PBS-tween and blocking by PBSK [PBS containing 1% bovine serum albumin (Roche, Mannheim, Germany) and 0.2% skim milk (Easis ...
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bioRxiv - Cancer Biology 2021Quote: ... tumor tissues up to 1 g were mechanically minced and digested in 2 ml RPMI1640 with 200 µg/ml Liberase TL (Roche by MilliporeSigma), 200 µg/ml Liberase DL (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... washed once with PBS and suspended in Homogenization Buffer (HB: 250 mM sucrose, Sigma-Aldrich; 10 mM HEPES pH 7.4, Sigma-Aldrich; 1 mM EDTA, Sigma-Aldrich, 2 mM PMSF, Roche). Homogenization was performed by a single freeze/thaw cycle ...
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bioRxiv - Plant Biology 2021Quote: ... The final clean nuclear pellet was resuspended in 1 mL nuclear lysis buffer (20 mM Tris pH 8, 2 mM EDTA, 0.1% SDS, 1 mM PMSF, 1X Roche protease inhibitor cocktail) and sonicated on the Covaris E220 (150W peak power ...
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bioRxiv - Developmental Biology 2022Quote: ... sections were blocked with 10% HI-serum for 2 h and incubated overnight at 4°C with alkaline phosphatase-labeled sheep anti-dig antibody (11093274910, Roche; 1:2500). After washing ...
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bioRxiv - Genetics 2020Quote: Cell pellets from THP-1 cells were suspended on ice-cold lysis buffer (PBS containing 2% SDS and complete protease inhibitor cocktail (Roche, catalog 11697498001)) ...
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bioRxiv - Microbiology 2022Quote: ... was used at a 1:1000 dilution to detect specific anti–SARS-2 immunoreactivity using the Discovery ULTRA automated staining instrument (Roche Tissue Diagnostics) with a Discovery ChromoMap DAB (Ventana Medical Systems ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The left lobe minced into small (~1-2 mm) sections and digested using 0.5 mg/mL collagenase D (Roche Diagnostics, Mannheim, Germany) and 50 U/mL DNase I (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed by addition of 250 μl lysis buffer (50 mM Tris/HCl, 500 mM NaCl, 2% w/v SDS, add freshly 1 mM DTT, 1x Roche inhibitor cocktail) followed by incubation on ice for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was sonicated in homogenization buffer (1% SDS, 50 mM Tris pH 7.4, 2 mM EDTA, protease and phosphatase inhibitors (Roche 11873580001; Roche 04906837001), heated at 95° C for 5 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... twice with High Salt Wash Buffer (500 mM NaCl, 0.1 % SDS, 1 % Triton X-100, 2 mM EDTA, 20 mM Tris-HCl 8, 1x Roche protease inhibitor mixture), and twice with TE wash buffer (10 mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2024Quote: Beads were then washed twice with Low Salt Wash Buffer (150 mM NaCl, 0.1 % SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH 8, 1x Roche protease inhibitor mixture), twice with High Salt Wash Buffer (500 mM NaCl ...
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bioRxiv - Cell Biology 2024Quote: Human peripheral blood mononuclear cells isolated from buffy coats were activated in vitro for 1 week with 20 U/ml of IL-2 (Teceleukin; Roche, Nutley, NJ) and 5 μg/ml phytohemagglutinin (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was resuspended in Buffer B (10 mM Pipes, 50 mM KCl, 5 mM MgCl2, 1 mM DTT, 2 mM PMSF, Roche protease inhibitor) with 1% Triton X-100 and incubated on ice for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... Pellets were resuspended in 120 μl lysis buffer (0.1 M NaOH, 0.05M EDTA, 2% SDS, 2% beta-mercaptoethanol, PhosStop (Roche), cOmplete protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cell Biology 2019Quote: ... 20 mM HEPES pH 7.4 with 2 mM 2-mercaptoethanol and EDTA-free cOmplete protease inhibitor tablets (Roche) prior to lysis by probe sonication ...
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bioRxiv - Cell Biology 2019Quote: ... 20 mM HEPES pH 7.4 with 2 mM 2-mercaptoethanol and EDTA-free cOmplete protease inhibitor tablets (Roche) prior to lysis by probe sonication ...
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bioRxiv - Biochemistry 2020Quote: ... blocked in 3% BSA and probed with anti-HA (clone 12CA5, Roche), anti-cMyc (clone 9E10 ...
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bioRxiv - Neuroscience 2020Quote: ... Following resuspension in (3 ml) homogenization medium containing protease inhibitor (Roche Diagnostics), the cells were cracked open using a ball bearing homogenizer with a 0.2507-inch bore and 0.2496-inch diameter ball ...
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bioRxiv - Systems Biology 2020Quote: ... The tissue was digested with Liberase Blendzyme 3 recombinant collagenase (Roche Diagnostics) according to the manufacturer instructions ...