Labshake search
Citations for Roche :
1051 - 1100 of 1954 citations for Centromere Protein O CENPO Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: 5 μl of antiserum were diluted with 250 μL of PBS containing 0.05% NP-40 and coupled to 10 μl of Protein A-Agarose beads (Roche) by rotating at 4°C overnight ...
-
bioRxiv - Microbiology 2019Quote: ... 1 mM ATP (+ 2 mM DTT for Strep-KaiC36803 proteins)] including protease inhibitors (protease inhibitor cocktail, Roche or Nacalai). Soluble proteins were loaded on self-prepared columns packed with Strep-Tactin XT superflow or Strep-Tactin Sepharose (IBA lifesciences ...
-
bioRxiv - Cell Biology 2020Quote: ... Cell lysates - non-transfected or overexpressing EGFP-tagged proteins -were prepared in lysis buffer (20mM HEPES, 1mM EDTA, 0.2% Triton X-100 and protease inhibitor cocktail (Roche)) briefly sonicated (three times 5 second pulses with 30 seconds rest ...
-
bioRxiv - Epidemiology 2019Quote: ... and C-reactive protein (CRP) was measured by automated particle-enhanced immunoturbidimetric assay (Roche UK, Welwyn Garden City, UK). CRP levels were measured at nine ...
-
bioRxiv - Cell Biology 2019Quote: ... the proteins were blotted onto a nitrocellulose membrane and probed with anti-GFP (1:1000; Sigma-Aldrich and Roche), anti-dgt6 (1:500 ...
-
bioRxiv - Microbiology 2021Quote: ... 200 μg each lysate was pre-cleared with 20 μl of Protein A agarose beads (3 mg/ml, Roche) in 200 μl for 1 hour at 4°C on a Labquake Rotator (Barnstead Thermolyne) ...
-
bioRxiv - Microbiology 2021Quote: ... C-reactive protein and D-dimer levels were measured using Cobas Integra 400 plus analyzer (Roche Diagnostics Nederland B.V.).
-
bioRxiv - Cell Biology 2021Quote: The protein contents were extracted utilizing an RIPA lysis buffer (Beyotime, Shanghai, China) and protease inhibitor (Roche, Basel, Switzerland). Next ...
-
bioRxiv - Cell Biology 2021Quote: Protein from 2D hPSC-cardiac cell cultures was extracted using RIPA lysis buffer supplemented with protease inhibitor cocktail (Roche). Protein lysate concentration was estimated using a BCA assay (ThermoFisher Scientific) ...
-
bioRxiv - Cell Biology 2022Quote: ... The protein lysate was electrophoresed into 6-10% SDS-PAGE gels and transferred to a PVDF membrane (Roche, 03010040001). After blocking with 5% BSA ...
-
bioRxiv - Genomics 2020Quote: ... Protein lysates were prepared by manually homogenizing frozen tissue in RIPA buffer containing protease and phosphatase inhibitor cocktails (Roche). Proteins were separated on a 4-20% TGX SDS-PAGE gel (Bio-Rad ...
-
bioRxiv - Pathology 2021Quote: ... 10 mM Na4P2O7) in the presence of protease inhibitors (completeTM, Mini, EDTA-free Protein inhibitor cocktail tablets, Roche, Sigma). Gut tissue and GDE were homogenized in the lysis buffer and sonicated three time for 30 seconds each at 10 μm amplitude ...
-
bioRxiv - Neuroscience 2020Quote: Proteins were extracted using Tris-buffered Saline (TBS) with 0.5% NP40 protein extraction buffer containing protease and phosphatase inhibitors (Roche), on ice ...
-
bioRxiv - Biochemistry 2019Quote: ... CaCl2 was added to a final concentration of 1.25 mM and incubated with 200 μl Anti-Protein C Affinity Matrix (Roche) for 4 hrs while rotating at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were harvested and ruptured with a Dounce homogenizer in the buffer A containing cOmplete protein inhibitor cocktail (Roche) and 1mM phenylmethylsulfonyl fluoride (PMSF) ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were obtained by lysing cells in PBS containing 0.1% Triton X-100 (PBS/TX) as well as protease and protein inhibitor cocktails (Roche) for 5 min on ice ...
-
bioRxiv - Biochemistry 2022Quote: ... The cell lysate was diluted to 0.8 mg/ml total protein concentration and 0.47% NP40 using dilution buffer (PBS plus protease inhibitor cocktail (Roche 04693159001) and precleared using Invitrogen™ Dynabeads™ MyOne™ Streptavidin T1 magnetic beads for 2-4 hours at 4°C during gentle rotation ...
-
bioRxiv - Biochemistry 2022Quote: ... EDTA-free TCA buffer was used where phosphorylation of proteins was to be investigated by alkaline phosphatase-treatment (Roche) or Phos-tagTM (NARD Institute ...
-
bioRxiv - Microbiology 2023Quote: ... Expressed proteins in the cell-culture supernatant were purified using a cOmplete His-Tag Purification Resin (Roche, Cat# 5893682001) affinity column ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein was eluted with wash buffer containing 10 mM maltose and subsequently bound to c0mplete His tag resin (Roche) for 2 hours ...
-
bioRxiv - Cancer Biology 2023Quote: Protein were isolated by using RIPA buffer with protease inhibitor cocktail (cOmplete Protease Inhibitor Cocktail, Roche, Mannheim, Germany, 04693116001), and concentrations were measured using the Qubit Protein Assay Kit (Thermo Fisher Scientific ...
-
bioRxiv - Pathology 2023Quote: ... Approximately 30-50 μg of proteins were separated by 10% SDS-PAGE and transferred onto a PVDF membrane (Roche). The membrane was blocked with 5% non-fat milk in PBS for 1 h at room temperature and then incubated with the specific primary antibodies at 4°C overnight ...
-
bioRxiv - Plant Biology 2023Quote: ... Meristem-enriched samples or whole seedlings were ground in liquid nitrogen and homogenized in cold protein extraction buffer (50 mM Tris HCl pH 7.5, 10% glycerol, 1 mM DTT, 1% IGEPAL, 1× Roche EDTA-free protease inhibitor and 1× Roche phosphatase inhibitor) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Whole cellular protein was extracted with RIPA buffer (Serva electrophoresis) containing protease inhibitor cocktail and phosphatase inhibitor cocktail (Roche). Western blots were performed following routine protocols55 ...
-
bioRxiv - Microbiology 2023Quote: ... Expressed proteins in the cell-culture supernatant were purified using a cOmplete His-Tag Purification Resin (Roche, Cat# 5893682001) affinity column ...
-
bioRxiv - Biochemistry 2024Quote: ... resuspended in extraction buffer (SDS 2%, 50 mM Tris-Cl pH 7.4, 1 mM PMSF, 2x cOmplete protein inhibitor cocktail (Roche)) ...
-
bioRxiv - Cell Biology 2024Quote: ... Quantitative Western blot analysis was employed to determine concentration of recombinant protein using a recombinant GFP standard (Roche, 11814524001) with a defined concentration of 1 mg/ml ...
-
bioRxiv - Cancer Biology 2024Quote: ... or protein extraction (50 mM Tris-HCl, 1% NP40, 0.15 M NaCl, pH 8) supplemented with phosphatase (PhoStop, Roche) and protease inhibitors (Complete ...
-
bioRxiv - Cancer Biology 2024Quote: Total proteins were extracted from cultured NB cells using RIPA buffer supplemented with protease and phosphatase inhibitor cocktail (Roche). The cell lysates were pre-cleared with protein A or protein G beads (Santa Cruz Biotechnology ...
-
bioRxiv - Physiology 2021Quote: ... containing 25 ng/mL mouse anti-HA antibody conjugated with peroxidase (Roche, 12013819001) and 4% non-fat skim milk ...
-
bioRxiv - Cell Biology 2020Quote: ... Primary antibodies used in this study include rat anti-HA 3F10 (Roche #11867423001), mouse m2 anti-FLAG (Sigma #F1804) ...
-
bioRxiv - Genetics 2021Quote: ... the slides were incubated with anti-DIG antibody (1:1000; Roche, Penzberg, Germany) overnight ...
-
bioRxiv - Developmental Biology 2020Quote: ... Probes were detected using an anti-digoxigenin antibody coupled to alkaline phosphatase (Roche) and NBT/BCIP (Roche ...
-
bioRxiv - Cell Biology 2022Quote: Antibodies used in this study were: GFP (clones 7.1 and 13.1; 11814460001; Roche) (1:2000 for WB ...
-
bioRxiv - Developmental Biology 2019Quote: ... Embryos were transferred to blocking buffer containing anti-digoxigenin antibody (Roche 1:2000) at 4°C overnight ...
-
bioRxiv - Cell Biology 2019Quote: ... Primary antibodies used in this study are as follows: mouse anti-GFP (Roche) and mouse anti-Actin (Sigma) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... anti-HA high affinity rat monoclonal antibody (Roche USA. Clone 3F10. Cat#: 11867423001).
-
bioRxiv - Developmental Biology 2019Quote: ... The samples were washed and incubated with an anti-Dig antibody (Roche #11093274910) o/n at 4°C ...
-
bioRxiv - Developmental Biology 2019Quote: ... Samples were incubated with alkaline-phosphatase-labelled anti-Digoxigenin antibody (1:2000, Roche) over night at 4°C ...
-
bioRxiv - Immunology 2019Quote: ... RNA FISH signal was detected by incubating FITC-labeled anti-digoxygenin antibody (Roche) in 4× SSC ...
-
bioRxiv - Pathology 2020Quote: ... the tissue sections were incubated with a universal secondary antibody (Roche Diagnostics KK) for 20 min at 37 °C and then visualized by the DAB Map detection kit (Roche Diagnostics KK) ...
-
bioRxiv - Biochemistry 2019Quote: Some samples were further affinity purified using an HPC4 antibody-conjugated resin (Roche), but yields from the HPC4 column were low ...
-
bioRxiv - Molecular Biology 2019Quote: Membranes were incubated with primary antibodies: anti-HA (Roche, #11666606001, 1:1000 dilution), anti-6X His tag (Abcam ...
-
bioRxiv - Developmental Biology 2020Quote: ... 0.1% Tween-20) containing an AP-labelled anti-DIG antibody (1/2000) (Roche). NBT/BCIP was used as a substrate for the Alkaline Phosphatase ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... anti-fluorescein antibody conjugated with POD (1:1000 in blocking solution) (11426346910, Roche) was added to the samples for overnight incubation at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... sections were incubated with peroxidase-conjugated anti-fluorescein antibody (1:500, Roche Diagnostics) and TSA Plus Fluorescein amplification kit (PerkinElmer ...
-
bioRxiv - Neuroscience 2020Quote: ... sections were incubated with peroxidase-conjugated anti-DIG antibody (1:500, Roche Diagnostics) and TSA Plus Cy3 amplification kit (second step ...
-
bioRxiv - Neuroscience 2021Quote: ... or anti-GFP mouse monoclonal antibody (1:200, 11814460001, clones 7.1, 13.1, Roche), respectively ...
-
bioRxiv - Biochemistry 2021Quote: ... 4A the following antibodies were used: mouse anti-HA 12CA5 1:1000 (Roche), rat anti-HMR 2C10 1:25 (Helmholtz Zentrum Munich) ...
-
bioRxiv - Microbiology 2020Quote: ... Fifty picomolar DNA were incubated with 50pM anti-digoxigenin-covered beads (antibody: Roche, #11093274910 and carboxylate-modified beads F1-XC030 ...