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Citations for Roche :
1051 - 1100 of 6830 citations for 4 Chloro 6 fluorobenzene 1 3 diamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Indexing PCRs were done with 3 cycles less than the determined qRT-PCR cycle threshold (Ct) using KAPA HiFi HotStart ReadyMix (KAPA Biosystems) with final custom Illumina I7 and I5 concentrations at 1 μM ...
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bioRxiv - Cancer Biology 2023Quote: ... The gel plugs were incubated at 50°C for 3 days and treated with fresh proteinase K at 20 mg/ml concentration (Roche Diagnostics), every 24 h ...
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bioRxiv - Immunology 2023Quote: ... coated with 2.5ug/mL aCD3 and re-stimulated for an additional 3 days in complete IMDM-10 supplemented with 50U/mL IL-2 (Roche 11011456001). For Th17 polarizations ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 10 mM Tris pH 8, 3 mM MgCl2, 0.5% NP-40, 0.15 mM spermine, 0.5 mM spermidine, Roche EDTA-free protease inhibitor) and incubated on ice for 20 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... was performed by cannulation of the trachea and gentle instillation/aspiration (3 times) of 1.0 ml of PBS with protease inhibitor cocktail tablets (Roche, Indianapolis, IN). The lavage fluid was centrifuged at 4000 rpm for 5 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... After homogenizing the tissue in ice-cold PBS with protease inhibitors (1mM PMSF; Phosphatase Inhibitor Cocktail 3, Sigma-Aldrich; Complete, Roche), the extracts were crosslinked with 1% formaldehyde for 10 minutes at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... cells at 40% confluency were transfected with 1 µg of mammalian expression plasmid DNA containing gene of interest using 3 µL X-tremeGENE 9 DNA transfection reagent (Roche, 6365809001) diluted in 100 µL 1x OPTI-MEM I reduced serum medium (Gibco ...
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bioRxiv - Systems Biology 2024Quote: ... (after a 3-hour fasting period) was measured by taking blood from the tail tip using an Accu-Chek glucometer (Roche Diagnostics). Mice then received an intraperitoneal (ip ...
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bioRxiv - Plant Biology 2023Quote: ... Meristem-enriched samples or whole seedlings were ground in liquid nitrogen and homogenized in cold protein extraction buffer (50 mM Tris HCl pH 7.5, 10% glycerol, 1 mM DTT, 1% IGEPAL, 1× Roche EDTA-free protease inhibitor and 1× Roche phosphatase inhibitor). The homogenate was centrifuged and the protein concentration of the supernatant was measured using a Pierce 660 nm Protein Assay Reagent (Thermo Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride, 0.05% NP-40, 10% glycerol, 1×Roche protease inhibitor cocktail). Cell lysates were prepared by bead-beating ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM EDTA, 1 mM EGTA, 1.5 mM MgCl2, 1% Triton X-100, 10% Glycerol, 1 mM DTT, Roche complete protease inhibitor) with 0.5 mg/mL Lysozyme and placed on a roller for 45 minutes at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 150 mM NaCl, 1 mM EDTA, 1mM EGTA, 1% Triton X-100, 0.4% SDS, 1% NP-40, 1× Roche protease inhibitor cocktail), one time with wash buffer B (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 1 mM PMSF, 0.05% NP-40, 10% glycerol, 1×Roche protease inhibitor cocktail) and were lysed by beating with 0.5-mm-diameter glass beads using a FastPrep instrument at a speed of 6.5 m/s for three cycles of 20 seconds each ...
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bioRxiv - Molecular Biology 2020Quote: Cryosections (10μM) of ventricular tissue were fixed in 4% paraformaldehyde and stained with In-situ Cell Death Detection kit (Roche). Sections were imaged on a laser-scanning confocal microscope (LSM 510 ...
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bioRxiv - Genetics 2021Quote: ... Then they were incubated overnight at 4°C with primary antibodies diluted in a dilution buffer (0.5% blocking reagent (Roche), 2% fetal bovine serum ...
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bioRxiv - Developmental Biology 2020Quote: ... the embryos were washed and equilibrated in NTMT buffer followed by coloration with 4-nitro blue tetrazolium (NBT, Roche) and 5-Bromo-4chloro-3-indolyl-phosphate ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatants from each buffer were dialyzed in 25 mM Tris-HCl and 5 mM EDTA pH 8.0 overnight at 4°C and subsequently digested with 200 mg/ml pronase (Roche). Peptides were precipitated with 5% trichloroacetic acid (TCA ...
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bioRxiv - Cell Biology 2020Quote: ... 4 were purified according to the instructions of the manufacturer using the High Pure PCR Template Preparation Kit (Roche) followed by RNAse A digest and a final purification with the High Pure PCR Product Purification Kit (Roche).
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bioRxiv - Plant Biology 2021Quote: ... qPCR reactions were performed in 10 μl reactions containing 4 μl of LightCycler 480 SYBR Green I Master (Roche), 4 μl of PCR-grade water (Roche) ...
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bioRxiv - Neuroscience 2020Quote: ... The PBS was aspirated and 1 ml of homogenization buffer (320 mM sucrose, 4 mM HEPES NaOH, pH 7.4) supplemented with cOmplete EDTA-free protease inhibitor (Roche) and 1 mM ATP NaOH ...
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bioRxiv - Developmental Biology 2021Quote: ... WB following 4-20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) employed HRP-conjugated anti-HA antibody (Roche). Quantification was performed by Image J software.
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bioRxiv - Genomics 2021Quote: ... These cells were re-suspended in lysis buffer (4% SDS, 50 mM Tris-HCl [pH 8.0], 10 mM EDTA, 100 mM NaCl) with Protease inhibitor (Roche) and RNase inhibitor ...
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bioRxiv - Evolutionary Biology 2020Quote: ... tissues were incubated at 4°C in a solution of 0.05% alkaline phosphatase-conjugated DIG antibodies (Roche cat #11093274910) overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... on a rotating wheel at 4 °C and all buffers were supplemented with protease inhibitor cocktail (#11873580 001, Roche). Prior to elution ...
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bioRxiv - Developmental Biology 2021Quote: ... WB following 4-20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) employed HRP-conjugated anti-HA antibody (Roche). Quantification was performed by Image J software.
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bioRxiv - Molecular Biology 2020Quote: ... the subventricular zone of 4 mice from each genotype was micro-dissected and tissue digested using Liberase DH (Roche) and DNAse I (250U/ml ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes were incubated overnight at 4° C on a rotator with primary antibody in Western Blocking Reagent (Roche), if not stated otherwise ...
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bioRxiv - Neuroscience 2022Quote: ... the supernatant was incubated at 4°C overnight with 20 μl anti-HA antibody coated beads (Roche, Rotkreuz, Switzerland) at RT ...
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bioRxiv - Neuroscience 2022Quote: ... Cultured cells/tissues were lysed with 4% SDS in TEA buffer (50 mM Triethanolamine, 150 mM NaCl, pH=7.3) supplemented with protease inhibitor cocktail (Roche). Total 500μg protein was treated with 10mM TCEP for 30min and then incubated with 25mM NEM for 2 h at room temperature to block unmodified cysteine residues ...
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bioRxiv - Molecular Biology 2022Quote: ... the isolated skin was incubated overnight at 4 °C in a tube containing 10 mL of 1x Dispase (Roche) and 2x Pen/Strep (Sigma ...
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bioRxiv - Immunology 2022Quote: ... was added and cells were centrifuged at 350g for 7 min at 4°C and resuspended in PBS with 0.5% BSA (Roche) and 1% FBS (Sorting Buffer) ...
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bioRxiv - Biophysics 2022Quote: ... The transcription mixture was incubated at 37 °C for 4 h and treated with 50U/mL DNAse I (Roche) at 37 °C for 30 min ...
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bioRxiv - Plant Biology 2024Quote: ... the lysate was clarified by centrifugation at 12,000 x g and 4°C for 15 min and then filtered using a 0.45-µm syringe filter (Roche). The filtered lysate was passed over a gravity-flow column packed with 0.5 ml amylose resin (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... The remaining supernatant was diluted in 1 mL pre-warmed capturing buffer (0.5 M LiCl, 4 M urea, 100U Ribolock inhibitor, Thermo, 1x protease/phosphatase inhibitor, Roche) and 10 µL from an equimolar stock (10 µM ...
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bioRxiv - Neuroscience 2024Quote: The procedure involves decapitation of adult flies (sieving, ∼6,000 heads), pulverization, homogenization (320 mM sucrose, 4 mM HEPES, protease inhibitors (complete, 11873580001, Roche)) and differential centrifugation (from low speed to higher speed ...
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bioRxiv - Molecular Biology 2023Quote: ... Precipitants were washed with acetone and dissolved in SDS-PAGE sample buffer containing 4 mM Pefabloc SC (11429876001, Roche). Equivalent protein amounts were subjected to immunoblotting analysis.
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bioRxiv - Cancer Biology 2023Quote: ... for 8 min followed by post-coloration using Bluing reagent for 4 min at RT (Roche Diagnostic, Meylan, France). The slides were then dehydrated (ethanol and xylene ...
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bioRxiv - Immunology 2023Quote: ... The epidermis and dermis layers were separated after incubation at 4°C for 16h in 0.2 mg/mL dispase II (Roche). Epidermal layers were fixed in 4% Para-formaldehyde for 20 min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... The slides were washed and detection was performed at pH 9.5 by incubating in nitro blue tetrazolium and 5-bromo-4-cholro-2-indoyl phosphate solution (Roche) as per manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: SDGC-SEC-purified stress granule cores (strain JD1370) were incubated at 0.23 A260 units/mL with 2 or 4 units of RNase H (10786357001; Roche) in 40 μL of RNase H buffer (20 mM HEPES-KOH pH 8.0 ...
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bioRxiv - Immunology 2023Quote: Skin samples collected from the ventral neck area of 4-5 mice were minced in Hank’s balanced solution (HBSS) and incubated at 4°C overnight with 1.5mg/ml dispase II (Roche; 25766800). The next day ...
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bioRxiv - Microbiology 2024Quote: ... after which the cells were harvested by centrifugation and resuspended in in lysis buffer (PBS, 4% SDS, with Roche EDTA-Free Protease Inhibitor added as per manufacturer’s recommendation) ...
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bioRxiv - Cell Biology 2024Quote: ... followed by overnight incubation at 4°C with Anti-HA- Peroxidase (High Affinity, 50 mU/ml, Roche, ref: 12013819001) and HRP-conjugated anti-actin antibody (1:5000 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 X protease and 1 X phosphatase inhibitors (Roche), 10 % v/v glycerol) ...
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bioRxiv - Molecular Biology 2022Quote: ... HA tag (Roche 11666606001, 1:1,000 or 1:3,000), His tag (Abcam ab18184 ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mg.mL−1 SDS) with Complete protease inhibitors (Roche). Equal amounts of protein (BCA protein assay ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 1 mg ml-1 collagenase/dispase (Roche) and 0.1 mg ml-1 DNase I (Roche ...
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bioRxiv - Plant Biology 2022Quote: ... 1 mM DTT and 1 × protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cancer Biology 2023Quote: ... CD68 (clone KP-1 at 1:20, Ventana- Roche), FOLR2 (OTI4G6 at 1:200 ...
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bioRxiv - Immunology 2023Quote: ... supplemented with collagenase D (1 mg ml−1, Roche) and DNase I (0.25 mg ml−1 ...