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Citations for Roche :
951 - 1000 of 1028 citations for Human ACE2 18 615 Recombinant Protein Fc Tag CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: 293T cells to express FLAG-tagged human RSPH proteins transiently were lysed with 1% Triton X-100 in 1X HEPES buffer with EDTA-free protease inhibitor cocktail (Roche) by rocking at 4 °C for 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant was incubated with 5% v/v pre-immune rabbit sera and 20 μl of protein G-beads (Roche) for 1 h on a rotator at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 50 µl of co-IVT protein was added along with 5 μL (bed volume) of washed HA-beads (11815016001, Roche) in 300 μL binding buffer (25 mM HEPES-NaOH [pH 7.5] ...
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bioRxiv - Cancer Biology 2023Quote: ... or MRTX849 for 3 hours prior to protein isolation with RIPA buffer containing 1x cOmpleted EDTA free protease inhibitor (Roche) and 1x phosSTOP (Roche) ...
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bioRxiv - Biochemistry 2023Quote: ... CVF samples were normalized to a protein concentration of 1 mg/mL using sterile PBS and EDTA-free protease inhibitor cocktail (Roche) was added ...
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bioRxiv - Cancer Biology 2023Quote: Whole cell lysate proteins were extracted using RIPA buffer (Cat. #89901, Pierce) containing complete protease inhibitor cocktails (Cat. #04693116001, Roche) and phosSTOP (Cat ...
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bioRxiv - Cancer Biology 2023Quote: ... and real-time analysis of the thermal stability of the protein was performed by fluorescence reading (excitation: 465 nm, emission: 580 nm) on a Light Cycler 480 (Roche).
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bioRxiv - Molecular Biology 2023Quote: ... The extraction of total proteins was performed using the PIERCE RIPA buffer (Thermo, #89900) complemented with EDTA-free cOmplete PIC (ROCHE).
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bioRxiv - Molecular Biology 2023Quote: ... The concentration of SWR was quantified by in-gel staining using the SYPRO Orange dye against a known concentration gradient of bovine serum albumin protein (Roche).
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bioRxiv - Biochemistry 2024Quote: ... Lysates were first incubated with 5 µl anti-GFP antibody (in house) for 1 hour rotating at 4°C and subsequently mixed with 50 µl protein G-agarose (50% slurry, Roche) and incubated for 1 hour rotating at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Total proteins from T-ALL#1 cells were extracted with a RIPA buffer containing a cocktail of 1Xprotease inhibitors (11836145001, Roche) and 1X phosphatase inhibitors (P2850 ...
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bioRxiv - Immunology 2024Quote: Proteins were extracted from tissues or cells in radioimmunoprecipitation assay (RIPA) buffer (Beyotime) containing a complete protease inhibitor cocktail (Roche), resolved by SDSPAGE ...
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bioRxiv - Microbiology 2024Quote: ... total proteins (g/dL) and Albumin (g/dL) levels were assessed in a ROCHE Cobas 8000 auto-analyzer (Roche Diagnostic). The adiponectin (ng/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples (containing 20 μg of total protein) were mixed with loading buffer and separated on 12 % SDS-PAGE gels and transferred to PVDF membranes (Roche, Switzerland). After blocking with Tris-buffered saline with 0.1% Tween 20 and 5 % nonfat dry milk ...
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bioRxiv - Plant Biology 2022Quote: ... The resulting yeast extracts were used for immunoblot analysis using anti-Myc (MBL, Woburn, MA, United States) for bait proteins and anti-HA (Roche, Switzerland) for prey proteins ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein lysate of 10-20 μg was run on 10–15% SDS-PAGE gel and transferred toa PVDF membrane (Roche, USA). The membrane was incubated for 60 min at room temperature in 5% BSA solution ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed in M-PER mammalian protein extraction reagent (Thermo, MA, USA) with protease and phosphatase inhibitors (Roche, Basel, Switzerland), and proteins were quantified using protein assays (Bio-Rad Laboratories ...
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bioRxiv - Bioengineering 2020Quote: Western blotting was performed as previously reported.[26] Protein extracts were prepared using RIPA buffer containing EDTA protease (Roche Applied Science) and phosphatase inhibitors (Phostop Roche) ...
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bioRxiv - Neuroscience 2021Quote: ... mice and lysed on ice for 30 min in protein lysis buffer (1 mM Na3VO4, 1 mM NaF, complete protease inhibitor cocktail (Roche, USA), 1% Triton X-100 in PBS) ...
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bioRxiv - Plant Biology 2020Quote: ... Resulting yeast extracts were used for western blot analysis using anti-Myc HRP-DirecT (MBL, Woburn, MA, United States) for bait proteins and anti-HA (3F10)-HRP (Roche, Switzerland) for prey proteins.
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bioRxiv - Immunology 2021Quote: Secreted twin strep-tagged gp140-soc proteins in the harvested and filtered supernatant (1L) were supplemented with protease inhibitor tablets (Roche Diagnostics) to prevent protein degradation and 5 ml of BioLock biotin blocking solution (iba Life Sciences GmbH ...
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bioRxiv - Systems Biology 2019Quote: ... The cross-linked proteins-RNA were cut directly from the gel and incubated with 160 μg of Proteinase K (Roche, 3115801001) in 600 μl wash buffer 2 supplemented with 1% SDS and 5 mM EDTA at 55°C for 2-3 hours with mixing ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total proteins were extracted from RAW264 using radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitors (Complete Mini, Roche Diagnostics GmbH, Mannheim, Germany) and phosphatase inhibitors (PhosSTOP EASYPack ...
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bioRxiv - Immunology 2020Quote: ... 14 and 21 of life were homogenized in protein lysis buffer (Tris 50 mM, 2% SDS, 1mM Na3VO4) containing proteinase inhibitors (Complete Mini, Roche Diagnostics) and benzonase (Novagen ...
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bioRxiv - Biochemistry 2019Quote: ... cells were incubated for 48 h at 37°C in 5% CO2, harvested and lysed using M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, 78501) (with 1X Roche Complete Protease Inhibitor and 100 mM NaCl) ...
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bioRxiv - Cell Biology 2020Quote: ... The biotinylated protein was incubated with 50ug fresh mitochondria extraction in import buffer containing ATP regeneration system (Creatine kinase (Roche 10127566001), Creatine Phosphate (Sigma-Aldrich 10621714001 ...
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bioRxiv - Cell Biology 2022Quote: ... of siCon or siRictor NIH3T3 cells co-expressing Flag-NDRG1 WT were homogenized in 2% SDS + 5 mM DTT to retrieve proteins in solution supplemented with complete EDTA-free protease inhibitor (Roche, 11873580001) and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... 10 μg total proteins were separated on a 12% SDS-PAGE gel and transferred to the PVDF membranes (Roche, CA, USA) using an electro-transfer apparatus (Bio-Rad) ...
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bioRxiv - Plant Biology 2022Quote: ... and the supernatant containing the immunoprecipitated protein was resolved in a 10% SDS-PAGE gel and detected by an anti-HA antibody (Roche, 12158167001).
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were size fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred into a polyvinylidene difluoride membrane (Roche, Basel, Switzerland). Membranes were incubated (overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... Hand dissected ovaries from 100-150 flies (depending on the expressed protein) of appropriate genotypes were lysed in RIPA buffer supplemented with Complete Protease Inhibitor cocktail (Roche, 11836170001) and 20 mM NEM ...
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bioRxiv - Neuroscience 2024Quote: Weighed brain tissue samples were processed for protein assays by addition of 10X volume NP-40 buffer with added cOmplete Protease Inhibitor (Roche #04693132001) and PhosStop (Roche 04906837001 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg nuclear lysate was adjusted to 500 uL in Protein Incubation Buffer (PIB+++; 150mM NaCl, 50 mM Tris pH 8.0, cOmplete protease inhibitor (Roche, cat 04693159001), 0.25% NP-40 and 1 mM DTT) ...
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bioRxiv - Neuroscience 2023Quote: Proteins were detected by Western blot analysis after separating 30–50 μg of total protein lysate prepared in RIPA buffer with protease inhibitor cocktail (cOmplete™, Roche) on a 4– 15% Mini-PROTEAN® TGX™ Precast Gel (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... beads with protein were subjected to overnight digestion by adding 100 µl 5 ng/µl sequencing grade trypsin (Roche Diagnostic GmbH) in ammonium bicarbonate (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein lysates were prepared in radioimmunoprecipitation assay buffer supplemented with a protease inhibitor mixture (Roche Applied Science, IN, USA) and 2mM phenylmethylsulfonyl fluoride (Sigma-Aldrich) ...
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bioRxiv - Genetics 2023Quote: Whole-worm protein extracts were generated by sonication in radioimmunoprecipitation assay (RIPA) lysis buffer with cOmpleteTM Protease Inhibitor Cocktail (Roche #4693116001) and β-glycerophosphate (Sigma-Aldrich G6251) ...
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bioRxiv - Bioengineering 2023Quote: ... lung tissues were thawed on ice and then resuspended in 0.5ml of Tissue Protein Extraction Reagent (T-PER) (ThermoScientific, cat# 78510) containing Complete Mini Protease Inhibitor Cocktail (Roche, cat# 11836170001). Tissues were homogenized using an electric handheld tissue homogenizer and were stored at -80°C until used for ELISA.
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bioRxiv - Molecular Biology 2023Quote: ... containing either single (for the IgG screen) or serial dilutions (for full IgG multi-point kinetics) of HEL protein (Roche #10837059001). For measurement of Fab binding kinetics ...
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bioRxiv - Systems Biology 2023Quote: ... medical ventricular drainage and normalization of CSF clinical chemistry analysis (normal CSF protein content lies in the 0.2-0.4 g/L range (Roche et al., 2008)) ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial lysis and protein digestion were performed simultaneously by adding 50 µL of 1 mg/mL trypsin (Roche Diagnostics, Mannheim, Germany) prepared in a 150 mM NH4HCO3 solution ...
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bioRxiv - Neuroscience 2023Quote: ... pH 7.4 with 0.1% Triton X-100) and then incubated with 350 µg protein in 1:1 8M urea with Complete protease inhibitor (Roche 11245200) sample lysis:Buffer A/0.5% Triton X-100 (150 mM NaCl ...
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bioRxiv - Biophysics 2024Quote: ... AF488-GAF-FL or AF546-GAF-ΔPOZ was flown through the protein channel diluted to 80 nM in imaging buffer (50 mM NaCl, 50g/mL BSA (Roche 10711454001), 0.05% NP40 (Sigma I8896) ...
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bioRxiv - Biophysics 2024Quote: ... Cultures were grown until an A600 of 0.6-0.8 was reached and protein over-expression was induced by incubating with 1 mM isopropyl-β-d-1-thiogalactopyranoside (IPTG, Roche) for 16 hours at 18°C.
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bioRxiv - Plant Biology 2020Quote: ... Western Blotting was performed on 14 day old seedlings to determine protein expression using anti-HA high affinity antibody (Roche, Penzberg, Germany). Primer sequences for RxL21 and RxL21 deletion variants are in Table S9 ...
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bioRxiv - Cancer Biology 2020Quote: ... protein lysates were prepared from HCC1428 cells with control or E2-E10 siRNA knockdown using modified Tissue Protein Extraction Reagent (TPER) (Pierce) and a cocktail of protease and phosphatase inhibitors (Roche Life Science). The protein lysates were then diluted into 0.5 mg/ml of total protein in SDS sample buffer and denatured on the same day ...
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bioRxiv - Cancer Biology 2020Quote: Whole-cell protein lysates were prepared by lysing cells in Radioimmunoprecipitation assay buffer (RIPA) supplemented with cOmplete Protease inhibitor (Roche, Basel, Switzerland) and PhosphStop (Roche) ...
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bioRxiv - Cell Biology 2021Quote: Total protein from cells or mouse tissues (n=3 per genotype) were extracted using the M-PER protein lysis buffer (ThermoScientific, Beverly, MA) containing protease inhibitors (Roche, Indianapolis, IN). Approximately 25 μg of total protein was electrophoresed on 4-12% SDS-PAGE gels and transferred to PVDF membranes ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with PBS and lysed (200 µl) with M-PER Mammalian Protein (ThermoScientific®) plus 1x Protease inhibitor (cOmplete Mini EDTA, Roche®). Cells were detached using a scraper (on ice) ...
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bioRxiv - Cell Biology 2021Quote: ... the pellet washed in 1x PBS then re-suspended in 1x protein loading buffer (4x NuPAGE® LDS sample buffer [Invitrogen], 1x PBS and 25 uL ß-mercaptoethanol, 2x Roche cOmplete Mini protease inhibitor cocktail tablets ...