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Citations for Roche :
701 - 750 of 980 citations for Human ACE2 18 615 Recombinant Protein Fc Tag CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: Proteins were extracted with 1× RIPA buffer containing 1× complete-mini protease inhibitor (Roche: 11836170001) and 1× phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... Spheroplasts were lysed by addition of 0.5% SDS and proteins degraded using proteinase K (Roche). SDS was precipitated using 1M potassium acetate and removed by centrifugation ...
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bioRxiv - Plant Biology 2022Quote: ... HA- and FLAG-tagged proteins were immunologically detected using HRP-conjugated anti-HA 3F10 (Roche) and anti-FLAG M2 (Sigma) ...
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bioRxiv - Physiology 2022Quote: Protein was extracted from frozen muscle in RIPA buffer supplemented with Complete protease inhibitor (Roche) with bead homogenization using a TissueLyser II (Qiagen ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: ... The cell lysate was pre-cleaned with protein G agarose beads (Roche Diagnostic, Basel, Switzerland) for 1 h at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... proteins of inputs were analyzed by Western Blot with the Anti-GFP from mouse (Roche) and Monoclonal ANTI-FALG® M2 antibody produced in mouse (Sigma Aldrich).
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bioRxiv - Bioengineering 2023Quote: MYOtissues proteins were extracted in RIPA buffer supplemented with Protease Inhibitor Cocktail EDTA-free (Roche) and Benzonase by homogenization ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with 2 mL of pre-equilibrated Protein C affinity resin (Roche) for 3h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Remaining lysate was rotated O/N with 25 µl of Protein-A-agarose beads (Roche) and 2 µg of mouse anti-HA antibody (Supplementary Table S1) ...
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bioRxiv - Biochemistry 2023Quote: ... Copurifying proteins were analyzed by Coomassie staining or Western blotting using antibodies against HA (Roche) or the CTD of Rpb1 (8WG16 ...
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bioRxiv - Cell Biology 2023Quote: ... and total protein extracted in ice-cold T-PER buffer containing protease inhibitors (Roche Diagnostics). Proteins were quantified by use of the BCA protein assay (Pierce ...
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bioRxiv - Immunology 2023Quote: ... Stimulated cultures were lysed in M-PER mammalian protein extraction reagent supplemented with PhosphoSTOP (Roche) and cOmplete Tablets (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... total protein extracts were prepared in lysis buffer supplemented with protease and phosphatase inhibitors (Roche). Proteins were quantified by bicinchoninic acid (BCA ...
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bioRxiv - Neuroscience 2024Quote: ... samples were captured with 50 µL of 50% (w/v) protein-A agarose beads (Roche), and the reaction was kept at 4°C for 3 hr on a rotary shaker ...
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bioRxiv - Developmental Biology 2024Quote: ... The remaining lysate was rotated overnight with 50 µl of Protein A-agarose beads (Roche) and 2 µg of mouse anti-HA (monoclonal ...
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bioRxiv - Bioengineering 2022Quote: ... Slides were blocked in 10% BSA and 0.05% Tween 20 in PBS for 30 minutes before application of one of the following antibodies: mouse anti-human p16INK4a (CINTEC-Roche, cat# 705-4793), rabbit anti-human alpha-smooth muscle actin or aSMA (Abcam ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK293 cells were transiently transfected with β-arrestin 2-Renilla luciferase 8 (Rluc8) and human C5aR2-Venus constructs using XTG9 (Roche, Sydney, Australia) for 24 h ...
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bioRxiv - Cancer Biology 2022Quote: ... frozen human samples were disrupted in 600 µl of lysis buffer containing green ceramic beads using the MagNA Lyser Instrument (Roche Life Science, Germany). The cDNA synthesis was performed in a 20-µl reaction using random hexamers ...
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bioRxiv - Microbiology 2022Quote: ... Quantification of beta-globin was performed by a commercially available human genomic DNA kit (The LightCycler Control Kit DNA, Roche Diagnostics, Basel, Switzerland)69.
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bioRxiv - Cell Biology 2020Quote: Protein was harvested in urea ammonium bicarbonate lysis buffer with complete EDTA free protease inhibitor (Roche) and phosphatase inhibitor (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... followed by incubation with 60 µg of Protein A- or G-agarose bead slurry (Roche, Germany) for 4 hours at 4 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... proteins were digested by addition of 40 µl proteinase K (Roche, dissolved to 10 µg/µl) and incubation for 2 hours at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Protein was extracted from cell fractions using RIPA buffer containing protease inhibitor complete EDTA free (Roche) and phosphatase inhibitor cocktail (Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... The FLUC-HA and NLUC-HA proteins were detected with anti HA-HRP antibody (Roche 3F10) (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and hybridized to a mouse monoclonal antibody against the P16 protein (Ventana Roche Diagnostics, E6H4, Switzerland) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Protein translation was induced with 1 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG, 11411446001, Roche Diagnostics) and cultures were incubated overnight at 18 °C ...
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bioRxiv - Plant Biology 2022Quote: ... Protein detection was carried out using peroxidase-coupled anti-HA-Peroxidase antibodies (Roche, dilution 1/4000), peroxidase-coupled anti-GFP antibodies (Milteneyi ...
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bioRxiv - Physiology 2021Quote: ... triglycerides and total protein were quantified using the Cobas c111 analyzer (Roche diagnostics GmbH, Mannheim Germany) as previously described by Segev-Hadar et al.38 ...
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bioRxiv - Molecular Biology 2022Quote: ... cell culture supernatants were harvested and proteins were purified from the supernatants using tandem Ni2+ (Roche) and Strep-Tactin (IBA ...
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bioRxiv - Molecular Biology 2019Quote: Total protein lysates were prepared using RIPA lysis buffer containing protease inhibitors (Complete Ultra Tablets, Roche). Equal amounts of total lysates were separated by SDS-PAGE (4–15% ...
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bioRxiv - Cancer Biology 2020Quote: Whole-cell protein lysates were harvested in lysis buffer (RIPA buffer supplemented with protease inhibitor (Roche), sodium vanadate and sodium molybdate ...
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bioRxiv - Biochemistry 2020Quote: ... coli cell pellets were resuspended in protein buffer containing cOmplete protease inhibitor cocktail (Roche, Basel, Switzerland), lysed by sonication and centrifuged for 45 min at 38,000 g to remove cell debris ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were subsequently detected with HRP-conjugated anti-HA antibody (rat monoclonal, Roche, catalog number 12013819001) or mouse monoclonal anti-GFP antibody (Novus Biologicals ...
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bioRxiv - Molecular Biology 2019Quote: ... and then Cas9 proteins were digested with 0.5 µl of proteinase K (∼20 mg/ml, Roche) at 37°C for 10 min ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The protein extraction was performed by adding 10 M urea containing complete protease inhibitor cocktail (Roche) and Halt™ Phosphatase Inhibitor (Thermo ...
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bioRxiv - Microbiology 2020Quote: ... the supernatants were incubated in presence of 20 µl of protein G agarose beads (Roche, 11243233001) for 3 h at 4°C on a rotor to reduce unspecific binding ...
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bioRxiv - Biophysics 2020Quote: ... The expressed protein is first purified by affinity chromatography using Ni-NTA coated agarose beads (Roche). His6-tagged proteins are eluted using 250 mM imidazole ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: ... Cell lysates (1 mg) were pre-cleared with Protein-G–agarose beads (Roche, catalog number 11719416001) for 1 h at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... Tagged SAC9 fusion proteins were revealed by using GFP monoclonal antibody (anti-GFP mouse monoclonal, Roche) and detected by chemiluminescence using ECL revelation as for T-PLATE and SH3P2 fusion proteins.
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bioRxiv - Molecular Biology 2022Quote: ... The clarified lysate was incubated with 2 mL of pre-equilibrated Protein C affinity resin (Roche) for 3 h at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... The extract was then precleared by incubation with 50 ul of Protein A-agarose beads (Roche) at 4°C for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... and homogenized in a T-PER tissue protein extraction reagent supplemented with protease (Roche Applied Science) and phosphatase inhibitors (Millipore) ...
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bioRxiv - Plant Biology 2023Quote: ... The extract was then precleared by incubation with 50 ul of Protein A-agarose beads (Roche) at 4°C for 30 min ...
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bioRxiv - Immunology 2023Quote: ... and equal amounts of protein from lysate were incubated with 5 μg anti-FLAG antibody (Roche) overnight ...
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bioRxiv - Biochemistry 2023Quote: ... protein eluates from every purification step were immediately supplemented with protease inhibitors (EDTA-free cOmplete, Roche) (together with DTT at 10 mM for cysteine-containing protein constructs) ...
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bioRxiv - Cancer Biology 2023Quote: Protein lysates were extracted with ice-cold RIPA buffer supplemented with protease and phosphatase inhibitors (Roche). Thirty μg of protein lysates were separated using SDS–polyacrylamide gel electrophoresis and transferred to a PVDF membrane (Immobilion-P ...
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bioRxiv - Cancer Biology 2023Quote: Whole-cell protein lysates were harvested in lysis buffer (RIPA buffer supplemented with protease inhibitor (Roche), sodium vanadate and sodium molybdate ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was incubated with 2 ml of pre-equilibrated Protein C affinity resin (Roche) for 2h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... HBE were processed for protein lysates and stored at -20°C (Complete Lysis-M Roche diagnostics). NET dosage was an average of extracellular dsDNA concentration (QuantiFluor ONE dsDNA System ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with 30 μl of pre-washed Protein A agarose bead slurry (#11134515001, Roche) for an additional hour (4 °C ...