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Citations for Roche :
9651 - 9700 of 9754 citations for Monoisodecyl Phthalate 100 Ug Ml In Mtbe Unlabeled Mono 3 7 Dimethyl 1 Octyl Phthalate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: Paired-end sequencing libraries for QTL-Seq analysis were prepared using >1 μg of pooled DNA with a KAPA HyperPrep PCR-free kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... then lysed on ice for 15 minutes in non-denaturing lysis buffer (150 mM KCl, 10 mM MgCl2, 5mM HEPES, 1% IGEPAL) supplemented with protease inhibitors (Roche Complete EDTA-free, Roche, Laval, Canada). The samples containing lysis buffer were then collected and spun down for 5 minutes in pellet the cell debris ...
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bioRxiv - Microbiology 2024Quote: ... the RNA-seq directional libraries were generated for 1 μg rRNA-depleted RNA using the KAPA Stranded mRNA-Seq Kit (Kapa Biosystems, KK8421). The rRNA-depleted RNA was fragmented ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Probes were detected by incubation overnight at 4°C in a 1:5000 dilution of Anti-DIG-AP antibody (Roche, Catalog# 11093274910) in Blocking Buffer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The probe was detected by incubating samples overnight at 4°C with a 1:1500 dilution of Anti-DIG-POD antibody (Roche, Catalog# 11207733910) in Blocking Buffer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... dissected samples were homogenized with a pestle in 1% sodium dodecyl sulfate in PBS supplemented with a protease inhibitor cocktail (cOmpleteTM mini, EDTA free, Roche, Basel, Switzerland). The resultant lysate was centrifuged at 20,000× g for 15 min ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked in MBST + 10% HISS + 2% BMB for 2 h at room temperature and incubated in alkaline phosphatase-conjugated anti-DIG antibody (1:4000, Roche, Cat#11093274910) diluted in blocking buffer until signal development ...
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bioRxiv - Molecular Biology 2024Quote: ... qPCR amplification of a 1:10 dilution of cDNA was performed in triplicate on a LightCycler® 480 II thermal cycler (Roche, France) using the LightCycler® 480 SYBR Green I Master mix and specific primers (Table S2) ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was resuspended in Buffer B (10 mM Pipes, 50 mM KCl, 5 mM MgCl2, 1 mM DTT, 2 mM PMSF, Roche protease inhibitor) with 1% Triton X-100 and incubated on ice for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... bacteria were harvested by centrifugation at 6,000 g for 10 min at 4°C and resuspended in L Buffer (25 mM Tris-HCl, 500 mM NaCl, 10 mM Imidazole, 1 mM PMSF, 5 % Glycerol, pH 8, containing Roche protease inhibitor cocktail). Bacteria were then lysed by sonication ...
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bioRxiv - Cell Biology 2020Quote: Mammalian cell lysates were prepared in radioimmuno-precipitation assay (RIPA) buffer supplemented with 1 tablet of EDTA-free protease inhibitor (Roche, Cat No. C762Q77) per 25ml buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then quickly washed twice in wash+ buffer (20 mM HEPES pH7.5 + 150 mM NaCl + 0.1% BSA + 0.5 mM Spermidine + 1 large Roche complete EDTA-free tablets in water). Samples were resuspended in 150 μl Wash+C (wash+ + 100 mM CaCl2 ...
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bioRxiv - Developmental Biology 2020Quote: ... Pelleted nuclei were resuspended in 333 μl of nuclear lysis buffer (50 mM Tris-HCl pH 7.5, 10 mM EDTA, 1% SDS, 1x Roche Complete protease inhibitors cocktail), kept 5 min on ice and diluted with 667 μl of ChIP dilution buffer (16.7 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... expressing polymerase module and SII-tagged Mpe1 were lysed in Buffer A (50 mM HEPES pH 8, 75 mM NaCl, 1 mM TCEP) supplemented with 3x protease inhibitor tablets (Roche, cat. No. 11836153001) and 1 ml BioLock ...
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bioRxiv - Genetics 2020Quote: ... After 24 hours cells were then fixed with a 1% (v/v) formaldehyde solution supplemented with cOmplete™ Mini EDTA-free Protease Inhibitor Cocktail (Roche®, UK) for 15 minutes with gentle agitation at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... Pellets were resuspended in 5 mL of membrane isolation buffer (5 mM TrisHCl pH 7.6, 5 mM MgCl2, 1 mM EGTA, protease inhibitor cocktail cOmplete [Roche Applied Science, Penzberg, Germany]) with a glass dounce tissue grinder (Wheaton ...
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bioRxiv - Molecular Biology 2019Quote: ... 250 mM sucrose, 5 mM MgCl2, 1 mM EGTA, 0.05% Tween-20, 0.5 mM DTT, 1x protease inhibitors [Roche], 0.4 u/μl RNase inhibitor). The nuclei or permeabilized cells were flash-frozen and stored at −80°C (10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... 72°C for 1 min) including the TruSeq primer mix and the KAPA Hifi Uracil+ Poly-merase Master Mix (Kapa Biosystems, Cat. #KK2801). The PCR product was purified and size controlled by Agilent Technologies 2100 Bioanalyzer (High Sensitivity DNA Chip) ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μm sections from the TMA-2 human adenocarcinoma cohort were duplex stained using a Ventana autostainer with antibodies for cytokeratin AE1/AE3 (1:250, #NCL-L-AE1/AE3, Leica detected with Roche discovery yellow chromogen) and SOX9 (1:10000 ...
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bioRxiv - Physiology 2020Quote: ... Inhibitors were added fresh on the day of use and included 1 cOmplete™ protease inhibitor cocktail EDTA free tablet (Roche, Basel, Switzerland) and Phosphatase Inhibitor Cocktail 3 both purchased from Sigma-Aldrich (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Cells were harvested and resuspended in lysis buffer A (50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 1 mM PMSF, and Roche EDTA free protease inhibitor). Following sonication and centrifugation ...
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bioRxiv - Microbiology 2019Quote: ... The obtained cDNAs were diluted 10 times in water and quantitative PCR was performed using 2.5 μL of each diluted cDNA in a total volume of 10 μL containing 1× LightCycler 480 SYBR Green I Master Mix (Roche Diagnostics, cat. #04707516001) and 800 nM of each primer in a LightCycler 480 System (Roche Diagnostics) ...
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bioRxiv - Cell Biology 2021Quote: ... and the cDNA template was digested for 30 min at 37 °C after the addition of 1 µl DNase I (10 U/µl; Roche Diagnostics, Rotkreuz, Switzerland), 5 µl 1M Tris/HCl buffer (pH 8.0) ...
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bioRxiv - Cell Biology 2022Quote: Cells were washed once with ice-cold PBS and stored at -80 °C or immediately lysed using lysis buffer (50 mM HEPES pH 7.4, 150 mM NaCl, 1% NP-40, 2x Roche EDTA-free protease inhibitors). Lysates were collected by scraping and were cleared by centrifugation at 16,100 g for 10 minutes at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: A total amount of 1 μg total RNA per sample was used for sequencing libraries generation by using KAPA mRNA HyperPrep Kit (KAPA Biosystems, Roche, Basel, Switzerland) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Plant Biology 2021Quote: ... total protein was isolated from Agrobacterium infiltrated leaves using extraction buffer (50mM Tris-Cl, 1% β-Mercaptoethanol and protease inhibitor cocktail (Roche, Basel, Switzerland)) ...
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bioRxiv - Plant Biology 2021Quote: ... pH 7.4) for 30 min and subsequently incubated 1 h with anti-HA-peroxidase high-affinity monoclonal rat antibody (3F10; Roche [catalog no. 12013819001]) diluted 1:1000 in 2.5% (w/v ...
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bioRxiv - Biochemistry 2020Quote: ... the expression medium containing the secreted fusion protein was supplemented with 1/4 tablet of protease inhibitor (complete EDTA-free protease inhibitor cocktail tablets, Roche Diagnostic GmbH, 45148300) and transferred to a 15 ml Falcon tube containing 200 μl of washed ...
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bioRxiv - Genetics 2022Quote: ... Samples were suspended in Laemmli sample buffer supplemented with a protease and phosphatase inhibitor cocktail (PIC; 1 mM PMSF, cOmplete™ Protease Inhibitor Cocktail [Roche, Base, Switzerland], PhosSTOP [Roche, Base, Switzerland]), and 10 mM N-ethyl maleimide (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... 2X SSC was replaced with 1X DNase buffer for 5 min and then a 1:50 dilution of DNase I in DNase buffer (DNase I recombinant, RNase-free, Roche, Cat. No. 04716728001), and incubated for 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The membrane was then incubated in antibody detection solution consisting of 1:20000 anti-HA rat mAb conjugated to horseradish peroxidase (HRP) (Roche [3F10], Cat. # 1201381900) diluted in MTBST 0.05% for 1 hour at room temperature on a tube rotator ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed in IP buffer (150 mM NaCl, 10mM Tris-HCl pH 8, 1 mM DTT, 0.2% NP-40, 1x protease inhibitor [Roche], 1x phosphatase inhibitor [Roche]), sonicated and centrifugated for 10 minutes at 14,000 RPM at 4°C ...
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bioRxiv - Plant Biology 2019Quote: ... The cDNA was diluted 20-fold and used for qRT-PCR employing a LightCycler® 480 SYBR Green 1 Master PCR labelling kit (Roche Applied Sciences) and RotorGene 3000 Real time PCR machine (Corbett Research ...
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bioRxiv - Neuroscience 2019Quote: ... 2X SSC was replaced with 1X DNase buffer for 5 min and then a 1:50 dilution of DNase I in DNase buffer (DNase I recombinant, RNase-free, Roche, Cat. No. 04716728001), and incubated for 1 hour at 37°C ...
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bioRxiv - Immunology 2019Quote: ... Reverse transcription of 1 µg RNA into cDNA was done using the Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics GmbH, Mannheim, Germany).
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bioRxiv - Cell Biology 2019Quote: Exponentially grown yeast cell cultures were pelleted and resuspended in yeast extraction buffer (50 mM HEPES, 150 mM NaCl, 1 mM EDTA) with 1x protease inhibitor cocktail (Roche Diagnostics, Penzberg, Germany). Cells were lysed by vortexing them together with glass beads (3-fold amount of glass beads and extraction buffer to pellet weight ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed twice using ice-cold D-PBS before scraping in ice-cold RIPA lysis buffer (50 mM Tris [pH 7.4], 150 mM NaCl, 1% NP-40 alternative, complete protease inhibitor and phosphatase inhibitor [Roche Applied Science, Mannheim, Germany]). The lysate was incubated on ice for 30 minutes with gradual agitation before clarification of samples by centrifugation at 10,000 ×g for 10 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: Pellets from exponentially grown cultures of Pea2-9myc expressing yeast cells were resuspended in yeast extraction buffer (50 mM HEPES, 150 mM NaCl, 1 mM EDTA) with 1x protease inhibitor cocktail (Roche Diagnostics, Penzberg, Germany), and vortexed together with glass beads (3-fold amount of glass beads and extraction buffer to pellet weight ...
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bioRxiv - Physiology 2020Quote: ... and immunodetection of the DIG-labeled hybrids was performed with a peroxidase-conjugated anti-DIG antibody (1:2000; raised in sheep; Roche Diagnostics, Mannheim, GER). The fluorescence signal was developed with tyramide signal amplification (TSA ...
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bioRxiv - Developmental Biology 2019Quote: ... sections underwent a series of saline sodium citrate buffer washes and were incubated with the anti-DIG-AP fab fragments (Roche, 11093274910, 1:1000) O/N at 4 °C ...
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bioRxiv - Systems Biology 2020Quote: ... The final pellet was resuspended in 150 μl of NTE buffer (137 mM NaCl, 1 mM EDTA, 10 mM Tris, pH 7.4) with protease inhibitor (Roche, cOmplete Mini tablets, 4693159001). In ultracentrifugation library experiments ...
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bioRxiv - Cell Biology 2019Quote: ... transferred to nitrocellulose (Hybond-ECL, GE Bioscience, Chalfont St.Giles, UK) and incubated for 1 hour in Western blocking reagent (Roche Applied Science, Basel, Switzerland). Primary antibodies (anti-human IL-17RA antibody ...
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bioRxiv - Neuroscience 2019Quote: ... pH 7.4, 150 mM NaCl, 1.5 mM MgCl2, 1 mM EGTA, 10% glycerol, and freshly added phosphatase inhibitors from Roche Applied Science Cat. # 04906837001). Lysed neurons were mixed 3:1 with 4X sample buffer (40% glycerol ...
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bioRxiv - Cell Biology 2020Quote: ... and then digested for 1 h at 37°C in a vigorously shaking solution consisting of 16 mg of Collagenase D (Roche, Pleasanton, CA, USA), 3.0 U of Dispase II (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, 5 mM EDTA, 50 mM NaF, 1 mM PMSF supplemented with Roche 1X Halt Protease inhibitor cocktail), incubated for 1 hour at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested and lysed using immunoprecipitation buffer (25 mM Tris pH 7.4, 150 mM NaCl, 0.4% NP40, 5% glycerol, 1X protease inhibitor cocktail from Roche and 1 mM of PMSF). Approximately 1.5 mg of lysate from each sample was incubated with brachyury antibody at 4°C for 4 h ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were pooled together and resuspended in 1.5 mL immunoprecipitation buffer (25 mM Tris pH 7.4, 150 mM NaCl, 0.4% NP40, 5% glycerol, 1X protease inhibitor cocktail from Roche and 1 mM of PMSF). Cells were then incubated for 30 minutes on ice prior to centrifugation at high speed (15,000 rpm ...
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bioRxiv - Neuroscience 2021Quote: Cells were washed in cold D-PBS prior to lysis on ice using lysis buffer (1% NP40, 100mM Tris pH8, 100mM NaCl, 10% glycerol, 5mM EDTA, cOmplete™ EDTA-free Protease Inhibitor Mixture, Roche Applied Science) supplemented with 20 mM N-ethylmaleimide ...
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bioRxiv - Developmental Biology 2021Quote: ... according to the manufacturer’s instruction and 1 microG of total RNA was reverse transcribed into cDNAs using the first-strand synthesis kit (Roche, Tucson, AZ, USA). Real-time quantitative PCR analyses were performed according to a previously published procedure 39 ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in SDS-PAGE loading buffer (50 mM TrisC1 pH 6.8, 2% SDS, 0.1% bromophenol blue, 10% glycerol, 4% β-mercaptoethanol, 1 mM PMSF, 1x Roche cOmplete protease inhibitor cocktail) and boiled for 10 min ...