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Citations for Roche :
9501 - 9550 of 9754 citations for Monoisodecyl Phthalate 100 Ug Ml In Mtbe Unlabeled Mono 3 7 Dimethyl 1 Octyl Phthalate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... The digested products were electrophoresed in 1% (w/v) agarose gel and then transferred onto positively charged Nylon membranes (Roche, Germany). For both assays ...
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bioRxiv - Cell Biology 2024Quote: ... The following antibodies were used for the preparation of stained gels for expansion microscopy: Rat anti-HA (3F10, 1:25, Roche 12158167001), Mouse IgG1 anti-alpha Tubulin Clone B-5-1-2 (1:500 ...
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bioRxiv - Biochemistry 2024Quote: ... 20,000g for 20 min and the pellet was resuspended in 50 µl of PBS supplemented with 1 mM TCEP and cOmplete protease inhibitor (Roche, 11836170001). Membrane proteins were solubilized and PEGylated by adding 7 µl of 10% β-d-dodecyl maltoside (Anatrace 329370010 ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were then incubated overnight at 4°C with a 1:2000 dilution of alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche #11093274910) in MABT blocking buffer with 1% sheep serum ...
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bioRxiv - Molecular Biology 2024Quote: ... then resuspended in 880 uL RIPA buffer (1x PBS, 1% NP-40, 0.5% Sodium deoxycholate, 0.1% SGS, Roche protease inhibitor cocktail), and sheared using Covaris E220 Focused-Ultrasonicator ...
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bioRxiv - Molecular Biology 2024Quote: The following antibodies and dilutions were used for western blotting or immunofluorescence: horseradish peroxidase-linked anti-HA antibody (Roche, 1:5,000), rabbit anti-INTS4 (Bethyl Laboratories ...
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bioRxiv - Microbiology 2024Quote: ... and cells were lysed on the plates for 30 min at 4 C in Lysis Buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.5% NP-40, PhosStop, Roche Complete Protease Inhibitor). Lysates were clarified by centrifugation at 13,000 RPM ...
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bioRxiv - Microbiology 2024Quote: ... 500 µL of lysis buffer II (Tris-HCl pH 4.7 50 mM, NaCl 150 mM, EDTA 1mM, Triton X100 1%, Roche Antiprotease Cocktail) was added and samples were sonicated for 30’ (Covaris ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were then incubated overnight at 4°C with a 1:2000 dilution of alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche #11093274910) in MABT blocking buffer with 1% sheep serum ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were gradually washed into SSC buffer and then into PBT before overnight incubation with an anti-DIG primary antibody at 1:5000 (Roche #11093274910). Embryos were washed in PBT and then staining buffer before developing in BM Purple staining solution (Roche #11442074001) ...
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bioRxiv - Immunology 2023Quote: ... Wells were pre-coated with 100 μL per well of capture anti-histone antibody contained in the Cell Death Detection ELISA kit (1:40 in 1x coating buffer; Roche 11544675001) or anti-myeloperoxidase (MPO ...
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bioRxiv - Immunology 2023Quote: ... 1 mM NaF) with EDTA-free protease inhibitors (cOmplete™ Tablets) and phosphatase inhibitors (PhosSTOP™, both Roche Diagnostics GmbH, Germany) for 1 hour at 4°C under rotation ...
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bioRxiv - Biochemistry 2022Quote: ... Total RNA-seq libraries were then prepared using 1 µg of RNA from each sample and the KAPA RNA HyperPrep Kit with RiboErase (Roche KR1351) according to the manufacturer’s protocol with 10 amplification cycles ...
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bioRxiv - Cell Biology 2023Quote: ... The minced tissue is lysed in tissue lysis buffer (50 mM Tris-HCl [pH7.5], 150 mM NaCl, and 1% NP-40 (11332473001, Roche Applied Science)) for 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: Weighed frozen tissue samples were processed for biochemical assays by adding 5X or 10X volume of chilled 1% NP40 + PBS homogenization buffer with added cOmplete Protease Inhibitor (Roche #04693132001) and PhosStop (Roche 04906837001 ...
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bioRxiv - Plant Biology 2023Quote: ... Primary and secondary antibody dilutions used for immune detection were as follows: α-HA(rat)-1:2,000 (Roche Diagnostics, Basel, Switzerland), α-GFP(mouse)-1:1500 (Roche Diagnostics ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA-seq libraries were prepared from 1 μg samples of total RNA using the KAPA RNA HyperPrep Kit with RiboErase (HMR) kit (KAPA Biosystems), and sequenced on the Illumina NovaSeq platform with 50-nt paired-end reads ...
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bioRxiv - Cancer Biology 2023Quote: ... resuspended in Wash Buffer (20 mM HEPES-NaOH pH 7.5, 150 mM NaCl, 0.5 mM spermidine, 1× Roche protease inhibitor cocktail), and counted using a Vi-CELL Blu cell counter ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were prepared from 2.5 ng of each sample using either the KAPA HyperPlus Kit (replicate 1: Roche/KAPA Biosystems, KK8512) or NEBNext Ultra II DNA Library Prep Kit for Illumina (replicate 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were prepared from 2.5 ng of each sample using either the KAPA HyperPlus Kit (replicate 1: Roche/KAPA Biosystems, KK8512) or NEBNext Ultra II DNA Library Prep Kit for Illumina (replicate 2 ...
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bioRxiv - Microbiology 2023Quote: ... Cross-linked cells were lysed by sonication in lysis buffer (5 mM EDTA, 1% NP-40 in PBS) supplemented with 2X cOmplete™ inhibitor (Roche). Cell lysates were treated with 2 units of Turbo DNase (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... the pellet was resuspended in 200 µL of cold buffer D (50 mM Tris/HCl, pH = 8.0, 10 mM EDTA, 1% SDS 1xPIC (cOmplete™, Protease Inhibitor Cocktail; Roche; 04693116001)) per 15 cm plate ...
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bioRxiv - Molecular Biology 2023Quote: ... the pellet was resuspended in 200 µl cold buffer D (50 mM Tris/HCl (pH=8.0), 10 mM EDTA, 1% SDS, 1xPIC (cOmplete™, Protease Inhibitor Cocktail (Roche; 04693116001)) per 15 cm plate ...
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bioRxiv - Molecular Biology 2023Quote: ... 250-500k cells were washed twice (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM spermidine, 1× Roche cOmplete protease inhibitors) and attached to 10 uL ConA-coated magnetic beads (Bangs Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with primary antibodies (5% milk, TBS-T, overnight, 4°C) at the following dilutions: rat anti-HA (1:1000; Roche Diagnostics), mouse anti-Myc (1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were rinsed with PBS and incubated with 5% (v/v) normal donkey serum and 1% (w/v) BSA (Roche, 10.7350.86001) in PBS for 1 hour at room temperature to block the non-specific antibody binding ...
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bioRxiv - Bioengineering 2022Quote: siRNAs and plasmids were transfected using DharmaFECT 1 transfection reagent (Dharmacon T-2001) and X-tremeGENE9 DNA transfection reagent (Roche 06365809001) respectively ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM DTT, 2 μM MG-132 proteasome inhibitor (#S2619, SelleckChem) and cOmplete® ULTRA EDTA-free inhibitor cocktail (#5892953001, Roche). Approximately 0.1 ml of 0.6 mm glass beads were added and thoroughly mixed ...
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bioRxiv - Cancer Biology 2023Quote: ... Then embryos were incubated overnight at RT in AP-conjugated anti-DIG antibody (Roche, 1:1000 diluted in the blocking buffer). On the third day ...
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bioRxiv - Developmental Biology 2023Quote: ... 100 μl of the lysis buffer was added to each 6-well plate along with 1×Protease Inhibitor Cocktail (PIC, Roche cOmplete). The cells were incubated in a rocker at 4°C for 1hr ...
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bioRxiv - Cell Biology 2022Quote: ... 5 mM EDTA, 10 mM β-mercaptoethanol, 10 mM Hepes pH 7.5, supplemented with 1× Complete protease inhibitor cocktail, Roche, Germany), passed through 27 G syringe and left 20 min in ice ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were harvested by centrifugation and resuspended in buffer A (20 mM Tris-HCl, 1 mM EDTA, pH 8.0) and protease inhibitors (Roche, Basel, Switzerland). α-Syn was then purified as previously described (62).
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bioRxiv - Developmental Biology 2023Quote: ... the transfected HELA cells were quickly washed in cold PBS twice and lysed in lysis buffer (Beytime, P0013) with Protease Inhibitor Cocktail (1/25 dilution, Roche, 04693116001) at 4°C for 30 min ...
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bioRxiv - Physiology 2024Quote: ... the samples were blocked with 5% skim milk in PBS-DEPC for 1 hour at 4°C and incubated with anti-DIG antibody (Roche Diagnostics) diluted 1:10,000 in the blocking buffer for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclear pellets were suspended in RIPA buffer (50 mM Tris-HCl, 150 mM NaCl, 1% Nonidet P-40, 0.5% NaDeoxycholate, 0.1% SDS, 1x Roche protease inhibitor mixture) and sonicated 5 times x 5 min (30 s ON/ 30 s OFF ...
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bioRxiv - Neuroscience 2023Quote: ... followed incubation with blocking solution for 2 hours before incubation with alkaline phosphatase (AP)-conjugated anti-DIG Fab fragments antibody (1:1000, Roche, #11093274910) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... 300,000 cells were washed twice (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM spermidine, 1× Roche cOmplete protease inhibitors) and attached to 10 ConA-coated magnetic beads (Bangs Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... An indexing PCR was performed to add Nextera index adapters (1 μM, Integrated DNA Technology) using KAPA HiFi reagents (Roche, #KK2102). Libraries were pooled in equal volumes and a final 0.8x cleanup performed with homebrew SeraMag beads before measuring sample concentration and quality ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed in SDS lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl pH 8) supplemented with Proteinase Inhibitor Cocktail (Roche, 11697498001) and chromatin was sonicated using the Bioruptor pico (Diagenode ...
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bioRxiv - Plant Biology 2023Quote: ... 50 mM Tris HCl pH 7.5, 10% glycerol, 10 mM EDTA, 10 mM DTT, 1mM NaF, 1% IGEPAL, 2X protease inhibitor, and 2X Roche phosphatase inhibitor) according to the sample quantity (approximately 200 µl of extraction buffer per 100 mg of ground plant material) ...
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bioRxiv - Plant Biology 2024Quote: ... The transformants were screened on MS plants with 1% sucrose and 0.8% agar containing either 40mg/L hygromycin (Roche, no. 10843555001) or 25mg/L DL-phosphinothricin (Duchefa ...
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bioRxiv - Genetics 2023Quote: ... Whole cell extract preparation and immunoblotting of Chlamydomonas cultures were performed as described previously (46, 48) with the following modifications: Antibody dilutions were: rat-anti-HA (1:5000) (3F10, Roche, Switzerland), Anti FLAG rabbit polyclonal (Rockland 600-401-383 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed in PBS and lysed in lysis buffer (150 mm NaCl, 1% IGEPAL CA-630, 50 mm Tris-HCl, pH 8.0, cOmplete EDTA-free Protease Inhibitor Mixture, Roche Applied Science) supplemented with 20 mm N-ethylmaleimide (NEM) ...
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bioRxiv - Genetics 2024Quote: ... Hybridizations of the probe with the RNA were detected with an alkaline phosphatase-conjugated antibody (1:5000; anti-DIG-AP, Fab Fragments, Roche, 11093274910). Finally ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed in PBS, incubated in 200ul lysis buffer (50mM Hepes pH 7.4, 150mM NaCl, 1mM MgCl2, 1% NP40, phosSTOP (Roche, Cat# 4906845001), protease inhibitor (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated at room temperature for 30 minutes with HRP-labeled sheep anti-FITC antibody (1/500 in TNB, Roche/Sigma). Following washes in TNT ...
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bioRxiv - Cell Biology 2024Quote: ... samples were covered with the staining mixture at 37°C for 1 h following instructions of the In Situ Cell Death Detection Kit (Roche, 11684795910). After the TUNEL reaction ...
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bioRxiv - Cancer Biology 2021Quote: ... Total cell lysates from COS-1 cells 24 hr post transfection were prepared using 300 μl of KAc interaction buffer (Roche Applied Science). GST fusion proteins were bound to glutathione-Sepharose beads (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were dissociated by incubation in DPBS (without Mg2+ or Ca2+) for 20 min at 37°C and then in 1:200 Liberase TH (Roche, Basel, Switzerland) in DPBS for 20 min at 37°C ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... Yeast pellets were resuspended in 2 x 500 μl of ice-cold lysis buffer (50mM Hepes, 150mM NaCl, 1% NP-40, 1mM EDTA) containing protease inhibitors (complete Protease Inhibitor Cocktail, Roche Life Sciences), 2 mM phenylmethylsulfonyl fluoride (Sigma-Aldrich ...