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Citations for Roche :
901 - 950 of 1269 citations for Recombinant Mouse SHH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Cells expressing GST fusion proteins were resuspended in PBS containing protease inhibitor cocktail (Roche Diagnostics, Penzberg, Germany) and lysed by lysozyme treatment (1 mg/ml ...
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bioRxiv - Bioengineering 2020Quote: ... and protein was extracted via Dounce homogenizer in a cocktail of RIPA buffer (Thermo) and proteinase (Roche) supplemented with phosphatase (Roche ...
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bioRxiv - Systems Biology 2019Quote: ... Proteins from fracture femora were extracted by RIPA buffer combined with protease inhibitors and phosphatase inhibitors (Roche). The supernatants were obtained by centrifugation (12,000g ...
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bioRxiv - Cell Biology 2020Quote: Whole cell or mitochondrial samples were lysed in RIPA buffer supplemented with cOmplete protein inhibitor cocktail (Roche) and 1mM phenylmethylsulfonyl fluorid (PMSF) ...
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bioRxiv - Genetics 2020Quote: ... we assessed levels of protein in mice urine using Chemstrip® 10 (Roche Diagnostics GmgH, Mannheim, Germany).
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bioRxiv - Microbiology 2020Quote: Protein was extracted from cells using Radioimmunoprecipitation assay (RIPA) lysis buffer containing 1X cOmplete Protease InhibitorCocktail (Roche) and 1X Phenylmethylsulfonyl fluoride (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Antibody protein treatments (anti-SARS-CoV-2 mAbs or human IgG1 isotype control Trastuzumab/Herceptin® (Roche)) were initiated 24 hours post infection by intraperitoneal injection ...
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bioRxiv - Biophysics 2022Quote: ... and 500-750 μg lysate was pre-cleared with Protein G agarose beads (Roche; Cat. No.11719416001) for 1 hour at 4°C with rotation at 5 rpm in a rotator ...
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bioRxiv - Plant Biology 2022Quote: ... For every biological replicate 500 μg protein was digested with sequencing grade trypsin (1:100 trypsin:protein; Roche) overnight at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... The expression of the 35S-driven IAR3 gene was monitored by protein immunodetection with anti-His (Roche) and anti-mouse IgG-peroxidase (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: Proteins were extracted using 1xPBS pH 7.4 with Protease Inhibitor Cocktail (1 tablet/10 mL) (Roche Diagnostics) and sonicated during 10 seconds at 10% power ...
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bioRxiv - Physiology 2023Quote: ... cells/tissue were lysed in mammalian protein extraction reagent (M-PER) supplemented with protease inhibitor cocktail (Roche). Soluble proteins and insoluble matrix were separated by centrifugation at 10,000 × g ...
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bioRxiv - Cell Biology 2023Quote: Testis protein extraction was performed in RIPA buffer with a cocktail of protease inhibitors (Roche cat. 11836170001), which was freshly added before beginning protein extraction ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were pre-cleared by incubating with 30 μl of protein G-agarose beads (Roche, cat. # 11719416001) for 3 h at 4°C on mini-rotator ...
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bioRxiv - Cell Biology 2023Quote: ... HA:SQSopsin was immunoprecipitated from the detergent-soluble fraction using 70 μl of 50% protein G resin (Roche) slurry and anti-HA antibody (12CA5) ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... Total protein was extracted with radio immune precipitation assay (RIPA) buffer containing phenylmethylsulphonyl fluoride (PMSF) (Roche, Switzerland) and a protease inhibitor cocktail (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... pH 7.5) was prepared with one tablet of protein inhibitor cocktail (cOMPLETE Mini lacking EDTA, 11873580001, Roche) along with 15uL of RNAsin (N2615 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein blots were probed with anti-HA antibodies conjugated to horseradish peroxidase (Roche, Switzerland, 12013819001, 1:4000). Immunoblots were visualised with Pierce ECL Plus Western Blotting Substrate (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: To extract total protein cells were incubated with RIPA buffer (CST) containing protease and phosphatase inhibitors (Roche) for 15 min on ice with occasional vortexing ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were lysed with 400uL RIPA buffer and prepared for total protein extraction with protease inhibitors (Roche). One mg of protein was precleared with A/G agarose beads for 30 minutes before PXR antibody (1.6μg ...
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bioRxiv - Molecular Biology 2024Quote: Transfected COS-7 cells were scraped in ice cold PBS containing Complete protein inhibitor cocktail tablets (Roche), centrifuged at 200 x g for 5 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... and organoids were scraped or collected in ice-cold PBS containing Complete protein inhibitor cocktail tablets (Roche) (unless otherwise stated ...
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bioRxiv - Neuroscience 2021Quote: Extracts from mouse brain were solubilized in pull-down buffer containing cOmplete™ Protease Inhibitor Cocktail (Roche) and 0.1% saponin for 45 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Gary Eitzen (University of Alberta)) and anti-GFP from mouse (clones 7.1 and 13.1; SKU 11814460001; Roche), and secondary antibodies ...
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bioRxiv - Plant Biology 2019Quote: ... the membrane was probed with a 1:20000 dilution of mouse anti-GFP antibody (JL-8, Roche) or mouse anti-alpha-tubulin antibody (B-511 ...
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bioRxiv - Developmental Biology 2019Quote: The following primary antibodies were used in the specified concentrations: mouse anti-GFP (1:1000, Roche #11814460001), rat anti-HA (1:1000 ...
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bioRxiv - Immunology 2021Quote: Mouse lung cells were obtained by digestion of lung tissue with 0.05 mg/ml Liberase TL (Roche) and 0.5 mg/ml DNaseI (Sigma ...
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bioRxiv - Neuroscience 2020Quote: DNA was extracted from mouse ear biopsies using High Pure PCR Template Preparation Kit (Roche, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies against the following antigens were used at the indicated dilutions: GFP (Mouse, 11814460001, Roche, 1/5000), HA (Rabbit ...
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bioRxiv - Genetics 2022Quote: Frozen mouse tissues were homogenized in CelLytic M cell lysis reagent (MilliporeSigma) and cOmplete protease inhibitors (Roche) was added to prevent protein degradation ...
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bioRxiv - Neuroscience 2021Quote: DNA was extracted from tail or ear clippings using the Kapa Mouse Genotyping Standard kit (KAPA Biosystems) and stored at -20°C ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated with 1:500 mouse anti-actin26 and 1:1000 rat anti-HA (3F10, Roche) followed by 1:1000 anti-mouse Alexa 488 (Invitrogen ...
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bioRxiv - Physiology 2019Quote: ... at RT followed by incubation with mouse anti-DIG alkaline phosphatase (AP) antibody (1:2000) (#11093274910, Roche) in Roche blockingbuffer (1:10 in PBT ...
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bioRxiv - Cell Biology 2020Quote: The primary antibodies used in this study were as follows: mouse anti-HA antibody (Roche Life Science), mouse anti-PGK1 monoclonal antibody (Thermo Fisher/Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were blocked with 3 % skim milk in TBS for 30 minutes and then probed with mouse anti-GFP (clone 7.1 and 13.1; 1:1,000, Roche) or rabbit anti-aldolase64 (1:2,000) ...
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bioRxiv - Immunology 2021Quote: ... mouse tissues were weighed and homogenized with zirconia beads in a MagNA Lyser instrument (Roche Life Science) in 1 mL of DMEM medium supplemented with 2% heat-inactivated FBS ...
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bioRxiv - Neuroscience 2020Quote: ... mouse monoclonal IgG1k anti-GFP (clones 7.1 and 13.1, 1:100 for immunoprecipitation, Roche, catalog no. 11814460001), IgG2b anti-GFP (clone GT859 ...
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bioRxiv - Cell Biology 2022Quote: ... and immunoblotted with a mouse monoclonal anti-HA antibody (clone 12CA5; Roche, 11 583 816 001, RRID:AB_514505). Rabbit monoclonal anti-PSTAIR (anti-Cdc2 ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... The primary and secondary antibodies used in western blot analysis were: mouse anti-GFP (1:1000, Roche), mouse anti-PLK4 (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The following primary antibodies were used: GCase (5 µg/mL; hGCase-1/23; mouse monoclonal; Roche [62]); LAMP1 (1:500 ...
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA (gDNA) was isolated from mouse ear punch biopsies using the PCR Template Preparation kit (Roche). Targets was PCR amplified using Taq Polymerase (Roche ...
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bioRxiv - Cell Biology 2024Quote: Antibodies used in this study were mouse monoclonal antibodies: GFP (clones 7.1 and 13.1; 11814460001; Roche, Germany) (1:2,000 for WB) ...
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bioRxiv - Cancer Biology 2023Quote: ... bound antibodies were detected with an anti-mouse HQ detection system (catalog# 7017936001 with 7017782001, Roche Diagnostics) and a Chromomap DAB IHC detection kit (catalog # 5266645001 ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was probed using the primary antibodies mAb mouse α-GFP (1:1’000, Roche Diagnostics #11814460001), mAb mouse α-PfGAPDH (1:20’000 ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by immunoblotting against GFP and against the loading control Pgk1 using mouse anti-GFP (11814460001, Roche), mouse anti-Pgk1 (459250 ...
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bioRxiv - Plant Biology 2024Quote: ... MAZ-GFP and MAZK141N-GFP accumulation blots were probed with mouse anti-GFP (1:5,000, Roche 1814460001) and goat anti-mouse-HRP (1:10,000 ...
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bioRxiv - Plant Biology 2024Quote: ... followed by 1 hour antibody incubation (α-GFP, monoclonal mouse antibody, Roche, catalog no. 11814460001, 1:1000). After three washes with TBST for 10 min each ...
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bioRxiv - Developmental Biology 2024Quote: ... Rabbit anti-ALFA (1:1000, NanoTag Biotechnologies, RRID:AB_3075998) and Mouse anti-HA (1:1000, Roche, RRID: AB_514505) primary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated for 30 min at 37 °C with mouse anti-BrdU (11296736, Roche. 1/10) then washed before incubation at room temperature for 3 h with the primary antibody anti-myosin heavy chain (MF20 ...
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bioRxiv - Cell Biology 2020Quote: ... Japan) was employed for detection of proteins visualized by Lumi-light Plus Western blotting substrate (Roche, Basel, Switzerland).