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Citations for Roche :
851 - 900 of 1269 citations for Recombinant Mouse SHH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... HBE were processed for protein lysates and stored at -20°C (Complete Lysis-M Roche diagnostics). NET dosage was an average of extracellular dsDNA concentration (QuantiFluor ONE dsDNA System ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with 30 μl of pre-washed Protein A agarose bead slurry (#11134515001, Roche) for an additional hour (4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Protein lysates were prepared from zebrafish larvae (6 dpf) in RIPA buffer containing protease inhibitors (Roche), using a manual dounce homogenizer ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins with HA-tag(s) were detected using rat mAb-anti-HA 3F10 (Roche, 1:1000) as primary antibody and IRDye® 800CW Goat anti-Rat IgG (Licor ...
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bioRxiv - Microbiology 2024Quote: ... The recognized protein bands were visualized by 3,3 N-Diaminobenzidine tertrahydrochloride (DAB) solution staining (Roche, Germany).
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bioRxiv - Developmental Biology 2020Quote: ... and sequential incubation with an anti-GFP primary antibody (mouse anti-GFP, 1:200, 11814460001, Roche) and a corresponding secondary antibody (goat anti-mouse Alexa Fluor 488) ...
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bioRxiv - Plant Biology 2019Quote: ... Jas9-VENUS and ACTIN were detected using mouse monoclonal antibodies anti-GFP 1:1,000 (11814460001, Roche) or anti-actin 1:2,000 (A0480 ...
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bioRxiv - Genetics 2019Quote: ... rat polyclonal anti-Lid (1:500) [13] and mouse monoclonal anti-GFP (Roche #118144600001; 1:200). Secondary antibodies were goat anti-rabbit antibodies (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryonic tail biopsies were used for rapid genotyping using KAPA Mouse Genotyping Kits (Kapa Biosystems, KK7352). All mouse protocols and experiments were approved by the Rutgers Institutional Animal Care and Use Committee ...
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bioRxiv - Immunology 2021Quote: ... mouse tracheas were surgically removed and placed in 10 ml 0.15% pronase solution (Roche, Basel, CH) and incubated overnight at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... The following primary antibodies were used at the indicated dilutions: mouse anti-Myc (Roche, 1:3000), goat anti-p75NTR (Neuromics ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used in this study: mouse monoclonal anti-GFP (1:2’000; Roche), anti-HA (clone 16B12 ...
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bioRxiv - Cell Biology 2021Quote: Antibodies used in this study were: mouse monoclonal antibodies GFP (clones 7.1 and 13.1; 11814460001; Roche) (1:2000 for WB ...
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bioRxiv - Microbiology 2021Quote: ... Dilutions for primary antibodies were: mouse monoclonal (mixture of clones 7.1 and 13.1) anti-GFP (Roche), 1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... the slides were incubated for 5 h at 8°C with mouse anti-DIG antibody (Roche, Basel ...
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bioRxiv - Microbiology 2019Quote: ... GRA5 blots were subsequently incubated with an anti-mouse secondary horseradish peroxidase (HRP)-conjugated antibody (Roche). The GRA7 blot was incubated with a secondary antibody (HRP)-conjugated anti-rabbit antibody (Roche) ...
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bioRxiv - Cell Biology 2020Quote: The lipid analysis of mouse plasma samples was performed using the COBAS Integra 400 Plus (ROCHE) and the following kits ...
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bioRxiv - Molecular Biology 2019Quote: ... The nitrocellulose membranes were blotted with either monoclonal mouse anti-GFP (11-814-460-001; Roche) or monoclonal mouse anti-HA (MMS-101R ...
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bioRxiv - Molecular Biology 2019Quote: ... DIG-labeled probes were detected using mouse monoclonal anti-DIG primary antibody (Roche; 1:100 dilution) and a Cy3-labeled goat anti-mouse IgG secondary antibody (Roche ...
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bioRxiv - Pathology 2020Quote: ... Quantitation of urinary albumin and creatinine was carried out using mouse albumin-specific ELISA kits (Roche) and creatinine determination kits (Enzymatic Method ...
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bioRxiv - Pathology 2021Quote: DNA extraction and PCR were performed using Kapa mouse genotyping kit (Kapa Biosystems, Wilmington, MA, USA) according to the manufacturer protocol ...
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bioRxiv - Microbiology 2021Quote: Mouse lungs were isolated and placed in RPMI1640 containing Liberase Blendzyme 3 (70 μg/ml; Roche) and DNase I (50 μg/ml ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... The antibodies used in immunoprecipitation were as follows: mouse anti-GFP (clones 7.1 & 13.1; 11814460001, Roche) and mouse anti-HA (F-7 clone ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... Western blot analysis was followed with an antibody against GFP (1:1000, mouse anti-GFP, Roche) and goat anti-Mouse IgG-HRP (1:3000 ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... The primary and secondary antibodies used in this analysis were: mouse anti-GFP (1:1000, Roche), rabbit anti-PLK4 (1:500) ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... was incubated with 1.5 μg of GFP antibody (mouse anti-GFP, clones 7.1 & 13.1; 11814460001, Roche), for 2 h on a rotating wheel at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... mouse thymuses were cut into small pieces and digested with 0.2 mg/mL DNase I (Roche) and 0.2 mg/mL collagenase P (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... mouse ventricular cardiomyocytes were freshly isolated by Langendorff perfusion with Liberase TM (0.225 mg/mL, Roche) in Krebs-Henseleit buffer (135 mM NaCl ...
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bioRxiv - Bioengineering 2023Quote: ... We also switched from mouse anti-HA to rat anti-HA (Roche catalog #3F10, 1:1000) to circumvent antibody cross reactivity issues observed with Olig2 and other cell type markers ...
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bioRxiv - Cell Biology 2023Quote: Mouse ventricular myocytes were freshly isolated by Langendorff perfusion with Liberase TM (0.225 mg/mL, Roche) in Krebs-Henseleit buffer (135mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... About 30mg of mouse TA muscle was added to MagNA Lyser Green Bead tubes (Roche 03358941001) with 600ul lysis buffer (PureLink RNA Mini Kit ...
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bioRxiv - Cell Biology 2023Quote: ... The antibodies used for immunoblotting were anti-GFP mouse monoclonal antibody (1:3,000 dilution, Roche, 11814460001) and anti-mCherry mouse monoclonal antibody (1:3,000 dilution ...
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bioRxiv - Immunology 2023Quote: Mouse lungs were isolated and placed in RPMI1640 containing Liberase Blendzyme 3 (70 μg/ml; Roche) and DNase I (50 μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatants were collected for Western blot analysis using a mouse antibody to GFP (1:2000, Roche) or mouse antibody to actin (1:5000 ...
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bioRxiv - Microbiology 2020Quote: ... Protein lysates were obtained by lysing cells in 0.1% SDS and protease inhibitor cocktail tablets (Roche #04693124001). Cell lysates were separated by SDS-PAGE (Bio-Rad 4-20% Mini-PROTEAN TGX Precast Gel ...
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bioRxiv - Molecular Biology 2019Quote: ... proteins were digested by addition of 40 µl proteinase K (Roche #03115852001, dissolved to 10 µg/µl) and incubation for 2 hours at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... proteins were digested by addition of 40 µl proteinase K (Roche #03115852001, dissolved to 10 µg/µl) and incubation for 2 hours at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 µl anti-CENP-ACnp1 (sheep in-house antiserum) and 25 µl Protein-G-Agarose beads (Roche) or 2 µl of anti-GFP (A11122 ...
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bioRxiv - Cell Biology 2019Quote: ... samples were further incubated for 2 hrs in 40 µl pre-cleared protein-G agarose beads (Roche). Beads with immunocomplexes were centrifuged at 3,000 × g and washed four times in lysis buffer with intermittent incubations ...
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bioRxiv - Genetics 2019Quote: Total protein was extracted from transfected HEK293 cells lysed in RIPA buffer (Pierce) containing protease inhibitors (Roche) 24 hours post transfection and size separated using SDS-PAGE ...
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bioRxiv - Biochemistry 2019Quote: ... and then scraped in 10 ml PBS + 1X Protein Inhibitor Cocktail buffer (catalog no. COUL-RO, Roche) and centrifuged for 5 min at 1,000 x g at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... The eluted proteins were subjected to 10% SDS-PAGE gels for immunoblot analyses using anti-GFP (Roche) and anti-Myc (Roche ...
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bioRxiv - Microbiology 2021Quote: ... transferred to PVDF membranes and protein expression was detected using anti HA (3F10, Roche 11867423001, 1:5000) followed by horseradish peroxidase (HRP)-conjugated anti-rat antibodies (Sigma ...
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bioRxiv - Cell Biology 2020Quote: Cells were lysed and proteins were purified by Complete™ Lysis-M EDTA-free kit (Roche, 04719964001). Protein samples were run on Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: Total proteins were extracted from cells using RIPA lysis buffer with a protease inhibitor cocktail (Roche, Germany). The proteins were quantified using Thermo Scientific Pierce BCA Protein Assay ...
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bioRxiv - Microbiology 2020Quote: Protein lysates of HEK-293T cells were extracted with RIPA buffer (PIERCE) and protease inhibitor cocktail (Roche). Loading buffer (Life Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were western blotted onto PVDF membranes and probed with the following antibodies: anti-HA (11867423001, Roche), anti-Flag (F3165 ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: Cells were harvested, lysed in RIPA buffer (Cell Signalling, 9806) containing a protein inhibitor cocktail (Roche, 11873580001), and centrifuged at 4°C for 10 min at 12,000 g/min ...
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bioRxiv - Molecular Biology 2020Quote: ... (a) Proteins were extracted from cryogrinded cell powders with different extraction solutions supplemented with protease inhibitors (Roche) (solution compositions listed in Supplementary Table 1) ...