Labshake search
Citations for Roche :
9001 - 9048 of 9048 citations for Cow WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... first purified with two rounds of polyA mRNA selection with oligo-dT magnetic beads and then converted to sequencing-compatible cDNA using the KAPA mRNA HyperPrep kit (KAPA biosystems, Cat. No. KK8580), using the KAPA single-indexed adapter kit for multiplexing (KAPA biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting cDNA was used for qRT□PCR analyses using the TransStart® Green qPCR SuperMix kit (AQ101-01, TransGen Biotech, Beijing, China) on a LightCycler® 480 System (Roche, Basel, Switzerland). Ubiquitin 5 was selected as an internal control ...
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bioRxiv - Microbiology 2023Quote: ... Final libraries were analyzed using Agilent DNA 1000 chip to estimate the quantity and check size distribution and were then quantified by qPCR using the KAPA Library Quantification Kit (ref. KK4835; Kapa Biosystems, Wilmington, MA, USA) prior to amplification with Illumina’s cBot ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Extracted DNA samples were sent to Admera Health (South Plainfield, NJ) and were prepared using the KAPA HyperPrep kit (Kapa Biosystems, Inc., Wilmington, MD) according to the provided instructions ...
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bioRxiv - Genomics 2024Quote: Genomic DNA (5∼8 μg) for Nanopore sequencing was end-repaired and ligated via a KAPA Hyper Prep Kit (Cat#KR0961, Kapa Biosystems, Wilmington, MA, USA), following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We verified enrichment success and measured size-adjusted DNA concentrations of each pool with qPCR using a SYBR-FASTqPCR kit (Kapa Biosystems, Wilmington, MA, U.S.A.) and a Bio-Rad CFX96 RT-PCR thermal cycler (Bio-Rad Laboratories ...
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bioRxiv - Genetics 2024Quote: ... Strand-specific and dual-barcode indexed RNA-seq libraries were generated from 450 ng total RNA each using the Kapa RNA-seq Hyper kit (Kapa Biosystems-Roche, Basel, Switzerland) and both the QIAseq FastSelect–5S/16S/23S ribodepletion and FastSelect rRNA Plant reagents (Qiagen ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of RNA from each sample was processed using the KAPA Stranded mRNA-Seq Kit with mRNA capture beads (Kapa Biosystems, Bedford row, London). Each sample was then eluted in 20 μL of elution buffer adjusted to 10 mM ...
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bioRxiv - Neuroscience 2023Quote: Libraries were prepared by the Van Andel Genomics Core from 500 ng of total RNA using the KAPA RNA HyperPrep Kit (Kapa Biosystems, Wilmington, MA USA). Ribosomal RNA material was reduced using the QIAseq FastSelect –rRNA HMR Kit (Qiagen ...
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bioRxiv - Physiology 2022Quote: Plasma TG and TC were measured after the dietary habituation period directly before the start of the training and after four weeks using the Total Triglyceride and Total Cholesterol assay kits (both Roche Diagnostics, Almere, The Netherlands), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... brain sections were processed for DNA strand breaks using Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) from Fluorescence In Situ Cell Death Detection kit (Roche Diagnostic, Indianapolis, IN, US) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2023Quote: ... To correct ONT reads another library using the same HMW sample as above was constructed for shotgun sequencing with the Hyper Library construction kit from Kapa Biosystems (Roche, Basel, Switzerland) and sequencing was performed using an Illumina NovaSeq at the Roy J ...
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bioRxiv - Cancer Biology 2023Quote: Libraries were prepared by the Van Andel Institute Genomics Core from 500 ng of total RNA using the KAPA RNA HyperPrep Kit (Kapa Biosystems, Wilmington, MA USA). Ribosomal RNA material was reduced using the QIAseq FastSelect –rRNA HMR Kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... were ligated to the individual cDNA products to barcode the samples and Kapa HiFi HotStart Library Amplification kit (kk2612, Roche Sequencing and Life Science) was used for library amplification.
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bioRxiv - Plant Biology 2023Quote: ... The PCR primer for generating the probe was 5’-GGTTCTGTGATTGAGTGTTTGGATCTCCCTGCG-3’ with a single-end CY3 tag generated using the DIG RNA Labeling Kit (Roche Applied Science, Penzberg, Germany) based on the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... mRNA was enriched by poly-A capture and mRNA-seq libraries were generated using KAPA Stranded mRNA-seq Kit (KAPA Biosystems, Wilmington, Massachusetts, USA). For ovulated mature eggs ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries for target enrichment of ∼3 Mb of Lupinus angustifolius genomic material were produced using the Roche Sequencing Solutions’ ‘SeqCap EZ – HyperPlus’ kit (Roche Sequencing Solutions, Pleasanton, CA) with 200 ng/L of input DNA.
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bioRxiv - Plant Biology 2023Quote: ... and purified based on the manufacturer’s instructions before sample barcoding with xGen Stubby Adapter and Unique Dual Index Primers (IDT) and library preparation using KAPA HyperPrep Kit (Kapa Biosystems-Roche, Wilmington, MA). The library was then sequenced by Illumina MiSeq nano PE150 at the Georgia Genomics and Bioinformatics Core ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections as previously described (46) (Iba-1 staining was conducted on a Ventana Benchmark using OptiView and UltraView detection kits provided by Roche (Roche Molecular Systems, Inc). Sections were deparaffinized in xylene and rehydrated through an ethanol series ending in distilled water ...
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bioRxiv - Zoology 2023Quote: ... Paired-end cDNA libraries were constructed from 4 μg of total RNA using a KAPA Stranded mRNA-Seq Kit (Kapa Biosystems, Inc., Wilmington, USA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: RT‒qPCR was conducted by using a KAPA SYBR Fast qPCR kit (Nippon Genetics) with a LightCycler 480 II system (Roche Applied Science, Penzberg, Germany). The PCR reactionwas conducted under the following conditions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and ligated to iTru adapters followed by limited-cycle PCR of iTru primers to add indexes and complete the library molecules using Kapa Library Preparation Kit reagents (Kapa Biosystems [Roche, Basel, Switzerland]). We sequenced the pooled libraries on an Illumina sequencer (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... We used identical input total RNA (50 ng to 500 ng) between control and samples for library preparation using the KAPA RNA HyperPrep Kit (KAPA Biosystems, Wilmington, MA, USA). Libraries were analyzed by Agilent 2100 bioanalyzer system for quality control ...
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bioRxiv - Immunology 2021Quote: ... Quality of libraries were determined via Agilent 2200 TapeStation using High Sensitivity D1000 tape and quantified using Kappa SYBR®Fast qPCR kit (KAPA Biosystems, Inc, Wilmington, MA). Approximately 60-80 million paired-end 150 np sequence reads were generated per sample using the Ilumina HiSeq 4000 sequencer.
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bioRxiv - Immunology 2021Quote: ... The tissues were then processed for immunohistochemistry using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760–159). All tissue slides were evaluated by a board-certified veterinary pathologist.
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bioRxiv - Microbiology 2021Quote: ... The tissues were then processed for immunohistochemistry using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760–159). All tissue slides were evaluated by a board-certified veterinary pathologist ...
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... The barcoded sequencing libraries were quantified by quantitative PCR using the KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems, Roche Holding AG, Basel, Switzerland). Sequencing libraries were loaded on a NextSeq500 (Illumina ...
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... The barcoded sequencing libraries were quantified by quantitative PCR using the KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems, Roche Holding AG, Basel, Switzerland). Sequencing libraries were loaded on a NextSeq500 (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... The tissues were processed for immunohistochemistry using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760–159) (Roche Diagnostics Corp. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: DNA was extracted from 1 mL of peripheral blood samples using a MagNA Pure LC DNA Isolation Kit in an automatic DNA extractor (MagNa Pure® System, Roche Applied Science, Indianapolis, Indiana). Thenceforth ...
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bioRxiv - Cell Biology 2020Quote: ... and 2.55 nL of 72 Ad153-reverse-tag (1~72) primer [1 μM] made by KAPA HiFi HotStart PCR Kit (KK2500, Kapa Biosystems, Cape Town, South Africa). Finally ...
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bioRxiv - Plant Biology 2021Quote: ... Library quality was assessed using a Fragment Analyzer and libraries were quantified by qPCR using the Kapa Library Quantification Kit (Kapa Biosystems, Inc, Wilmington, MA, USA). RNA-seq experiments have been performed on an Illumina HiSeq3000 using a paired-end read length of 2×150 bp with the Illumina HiSeq3000 sequencing kits.
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bioRxiv - Microbiology 2020Quote: ... DNA isolation was performed with a MagNA Pure LC total Nucleic Acid Isolation kit in the MagNA Pure® system (Roche Diagnostics, Almere, the Netherlands). Samples were tested with a Chlamydiaceae PCR targeting the 23S rRNA and C ...
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bioRxiv - Microbiology 2021Quote: ... The tissues were then processed for immunohistochemistry using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760–159). All tissue slides were evaluated by a board-certified veterinary pathologist and a pathology scored was assigned based on the following observations ...
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bioRxiv - Developmental Biology 2020Quote: ... either SP6 or T7 RNA polymerase was used to produce a DIG (digoxigenin)-labeled RNA anti-sense probe (Roche DIG RNA Labelling Kit (SP6/T7)) ...
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bioRxiv - Immunology 2021Quote: ... concentrations measured in 100 ul of basolateral medium incubated with the reaction mixture of a cytotoxicity detection kit (Sigma-Aldrich, Roche, Saint Louis, MO, USA) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... A LYS2 probe was amplified and labeled with Digoxigenin (DIG)-dUTP using the primers 5’-TGAAGCCTTCCCAGAGAGAA and 5’-GCCAAGGAAAAATGTCTACCA (Roche PCR DIG Probe Synthesis Kit). The DIG probe was hybridized to the membrane (at 44°C ...
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bioRxiv - Microbiology 2020Quote: ... The three PCR reactions per sample were combined and purified using the High Pure PCR Product Purification Kit of ROCHE™ (Roche Diagnostics GmbH, Mannheim, Germany). Sequencing was performed using the MiSeq™ Illumina® (2 x 300 bp ...
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bioRxiv - Immunology 2021Quote: ... The tissues were processed for immunohistochemistry using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760–159).
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bioRxiv - Immunology 2022Quote: ... The tissues were then processed for immunohistochemistry using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760– 159). All tissue slides were evaluated by a study-blinded board-certified veterinary pathologist.
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bioRxiv - Immunology 2023Quote: ... The tissues were then processed for immunohistochemistry using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760–159). Detection of SARS-CoV-2 viral RNA was performed using the RNAscope 2.5 VS assay (Advanced Cell Diagnostic Inc. ...
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bioRxiv - Plant Biology 2023Quote: First-strand cDNA was synthesized by reverse transcribing 1 μg of total RNA in a final reaction volume of 20 μl using MMLV reverse transcriptase (Roche High Fidelity cDNA synthesis kit) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The tissues were then processed for immunohistochemistry using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat. 760-159).
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bioRxiv - Microbiology 2024Quote: ... Quantitative RT-PCR was performed using the Qiagen kit QuantiFast® SYBR® Green RTPCR in a LigthCycler® 480 instrument (Roche Molecular Systems, Inc.) with mouse gene-specific oligonucleotides (Supplementary Table 1) ...
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bioRxiv - Immunology 2024Quote: ... spectrophotometer and 100 ng to 300 ng of total RNA was retrotranscribed to cDNA using the First Strand cDNA synthesis kit with random hexamers (Roche, catalog 04 897 030 001), or the High-Capacity cDNA Reverse Transcription Kit with RNAse inhibitor from Applied Biosystems (catalog ...
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bioRxiv - Cell Biology 2021Quote: ... staining was performed by autostaining on a Discovery Ultra Ventana using the Discovery OmniMap anti-Rb HRP and Discovery ChromoMap DAB kits (Roche, Cat # 760-4311 and 760-159).
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bioRxiv - Microbiology 2023Quote: ... no-chloroplast amplifying primers 335F (CADACTCCTACGGGAGGC) and 769R (ATCCTGTTTGMTMCCCVCRC) (Dorn-In et al. 2015) were used together with KAPA2G Robust PCR Kits (Kapa Biosystems, Cat. No. KK5005, Wilmington, USA). In protocol 2 (P2) ...
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bioRxiv - Plant Biology 2023Quote: ... and 1.5 ug RNA was used for library preparation at the High-throughput Sequencing Facility at UNC Chapel Hill using the Stranded mRNA-Seq kit (Kapa Biosystems. The NovaSeq 6000 sequencer (Illumina) generated 50bp paired-end reads with a read depth of 23-35 million reads per biological replicate ...