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Citations for Roche :
851 - 900 of 6811 citations for Recombinant Human Collagen Type II Alpha 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... PCR reactions were performed on a LightCycler 480 II Real Time PCR Instrument (Roche) and analyzed using LightCycler 480 Software ...
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bioRxiv - Immunology 2022Quote: ... Excised skin was placed in a 0.5% Dispase II solution (diluted in PBS; Roche) and placed at 4ºC overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR (qPCR) was performed using LightCycler® 480 II system (Roche Diagnostics, Switzerland) with the following conditions ...
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bioRxiv - Developmental Biology 2023Quote: ... and the resulting cDNA was examined by real-time PCR (LightCycler 480 II, Roche). The cycle threshold (Ct ...
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bioRxiv - Bioengineering 2023Quote: ... qPCR was performed in 384-well plates using the LightCycler 480 Instrument II (Roche). Target gene expression change was calculated relative to non-targeting controls using the ddCt method.
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bioRxiv - Pathology 2023Quote: ... The amplification of cDNA were performed by LightCycler 480 II (Roche Life Science, USA) with the following protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Real-time PCR was conducted on a Light Cycler 480 II (Roche, Mannheim, Germany) using ChamQ SYBR qPCR Mix (Q711 ...
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bioRxiv - Biochemistry 2023Quote: DSF measurements were taken using a Light Cycler 480 II instrument (Roche Applied Sciences). Protein samples were prepared in 20 µl containing 0.1 mg ml−1 SKP1/FBXO22 ...
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bioRxiv - Biochemistry 2024Quote: ... DSF was performed in a Light Cycler 480 II (Roche Applied Science, Penzberg, Germany) using a 4 °C/min temperature gradient from 20 °C to 95 °C ...
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bioRxiv - Microbiology 2023Quote: ... samples were cut into ≤5mm diameter pieces and incubated with dispase II (4942078001, Roche) for 2 h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... as per the manufacturer’s protocol on the LightCycler® II 480 system machine (Roche). Primer sequences are detailed in Table S2 ...
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Modular structure of RNA 3’ processing condensates involving the Arabidopsis RNA binding protein FCAbioRxiv - Molecular Biology 2024Quote: ... All the samples including inputs were subjected to qPCR analysis via LightCycler480 II (Roche). The data were normalized to 1% of input ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The real time PCR program of the quantitative PCR (LightCycler480 II, Roche, Basel, Switzerland) was arranged as follows ...
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bioRxiv - Physiology 2024Quote: ... HRM analysis was performed on the LightCycler 480 System II (Roche Diagnostics, Basel, Switzerland) using the LCGreen Plus dye (BioFire Defense ...
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bioRxiv - Genetics 2024Quote: ... and digested with NB4 collagenase (12mg/ml) (SERVA) and Dispase II (100U/ml) (Roche) for 1 hour ...
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bioRxiv - Developmental Biology 2024Quote: ... E2 levels were assessed using an E170 Modular (Gen II, Roche Diagnostics, Mannheim, Germany). To convert these E2 values ...
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bioRxiv - Developmental Biology 2021Quote: ... Seminiferous tubules retained on the filter were collected and incubated at 32 °C for 25 min in GBSS containing 1.2 mg/mL of Collagenase Type I and 5 μg/mL DNase (Roche 11284932). Cell aggregates were sheared gently by 10 rounds of pipetting with a wide orifice plastic transfer pipet and filtered through the Cell strainer to remove cell clumps ...
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bioRxiv - Biochemistry 2020Quote: ... Biotin-labeled CMV-RLuc-EV715’UTR (wild type or CCC mutant)-FLUC and biotin-labeled SL2 (wild type or CCC mutant) were synthesized using biotin-16-UTP (Roche). Binding reaction and pull-down were carried out as described5 ...
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bioRxiv - Microbiology 2021Quote: ... olfactory bulb and brain from each group of mice were pooled and treated with 400 U/ml type IV collagenase and DNase I (Roche) for a 30-minute incubation at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... RNA/DNA extraction from all material types was performed on the MagNA Pure 96 platform (Roche Diagnostics GmbH, Mannheim, Germany). Phocine Distemper Virus (PDV ...
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bioRxiv - Developmental Biology 2023Quote: ... dissected in ice-cold PBS and then digested with 4mg/ml Collagenase Type IV/ 0.2mg/ml DNase I /10% FCS/PBS under constant shaking with 700 rpm at 37°C for 12-15 min (Collagenase Type IV, Cat#: 17104-019, Gibco; DNase I, Cat#: 10104159001, Roche). Samples were filtered using a 70µm nylon filter and then washed twice with FACS buffer (0.5% FCS/2mM EDTA in PBS ...
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bioRxiv - Immunology 2023Quote: ... Lactate dehydrogenase (LDH) in the supernatants from either cell type was quantified using the LDH Cytotoxicity Detection kit (TaKaRa or Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from human samples using TriPure Isolation Reagent (Roche). RNA integrity (RIN ...
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bioRxiv - Genomics 2022Quote: ... the regions of interest were PCR amplified from human genomic DNA (Roche) using Taq polymerase (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: Human TGs were measured using the Cobas c 501 analyzer (Roche Diagnostic). For this purpose ...
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bioRxiv - Genetics 2023Quote: Candidate regions were PCR-amplified using human genomic DNA (Roche, Basel, Switzerland) as template and cloned in pGL4.23 Firefly luciferase reporter vector (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... eosin and antibodies for human keratins AE1/AE3 (760-2135; Roche diagnostics). Metastasis quantification was reached through a review of 17 digitalized slides ...
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bioRxiv - Developmental Biology 2021Quote: Digoxigenin-labelled sense and antisense RNA probes were prepared by in vitro transcriptions using recombinant plasmids of target genes made as mentioned above (Roche Life Science) and used for in situ hybridization ...
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bioRxiv - Neuroscience 2024Quote: ... brain slices were permeabilized by 0.3% triton in PBS and positive control slices were incubated with Dnase I recombinant (Roche, Basel, Switzerland, #4536282001) (1,000 units) ...
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bioRxiv - Developmental Biology 2024Quote: ... The pIEx-4-BmU6-Cad96ca-gRNA-Cas9-GFP-P2A-Puro or pIEx-4-BmU6-Fgfr1-gRNA-Cas9-GFP-P2A-Puro recombinant vectors were transfected into HaEpi cells with transfection reagent (Roche, Basel, Switzerland). After 48 h of vector transfection (cells can be observed to express green fluorescent protein) ...
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bioRxiv - Cancer Biology 2020Quote: ... HPV DNA capture was performed with NimbleGen’s SeqCap EZ Custom Library containing probes for all HR HPV types (Roche NimbleGen, Inc.,) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... We performed copy number qPCR using genomic DNA from three humanized and three wild type individuals using Light Cycler 480 SYBR Green I Master Mix (Roche #04707516001). The biological replicates for each individual were run in triplicate and error bars show the standard deviation from these technical replicates ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative real-time PCR was performed on the LightCycler 480 Instument II (Roche Life Science) using LightCycler480 SYBR GREEN I master (Roche Life Science) ...
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bioRxiv - Molecular Biology 2021Quote: ... Oligo dT(18)-primed reverse transcription was carried out with ImProm-II Reverse Transcriptase (Roche). Semi-quantitative radioactive PCR (RT-PCR ...
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bioRxiv - Molecular Biology 2021Quote: ... Oligo dT(18)-primed reverse transcription was carried out with ImProm-II Reverse Transcriptase (Roche). Semi-quantitative radioactive PCR (RT-PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... the sections were equilibrated with buffer II [Buffer I containing 0.5% Blocking reagent (Roche, Germany)] at room temperature for 1 h before incubation with alkaline phosphatase-conjugated anti-digoxigenin antibody (1:500 dilution) ...
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bioRxiv - Bioengineering 2019Quote: Quantitative real-time expression analysis was performed using a LightCycler®480 II instrument (Roche) equipped with 384 well plates and PowerUp™ SYBR™ Green Master Mix (Applied Biosystems) ...
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bioRxiv - Cell Biology 2019Quote: ... using a luciferase based bioluminescence assay (ATP Bioluminescence Assay Kit HS II, Roche Applied Science). 3 thoraces were used for one assay and 3∼4 assays were performed for each genotype.
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bioRxiv - Microbiology 2019Quote: ... The qPCR reactions were performed on a LightCycler® 480 II instrument (Roche Diagnostics, Switzerland) in two technical replicates on the reverse-transcribed RNA isolated from four biological replicates ...
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bioRxiv - Microbiology 2019Quote: ... The qPCR reactions were performed on a LightCycler® 480 II instrument (Roche Diagnostics, Switzerland) in two technical replicates on 50 ng template DNA extracted with a YeaStar Genomic DNA kit (Zymo Research ...
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bioRxiv - Genomics 2020Quote: ... on a Roche LightCylcer 480 Instrument II using LightCycler 480 SYBR GreenI Master (Roche, NJ). Primers were designed using Primer Blast ...
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bioRxiv - Microbiology 2020Quote: ... All qPCR experiments were performed in a LightCycler® 480 Instrument II (Roche Molecular Diagnosis), with the following conditions ...
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bioRxiv - Neuroscience 2019Quote: ... All samples were compared to DIG-labelled DNA molecular-weight markers II (Roche Applied Science) and repeat number was estimated compared to the DNA marker sizes.
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bioRxiv - Biochemistry 2021Quote: ... Quantitative real-time PCR reactions were carried out in a LightCycler 480 II system (Roche) in 384-well plates ...
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bioRxiv - Plant Biology 2020Quote: ... The HRM assay was performed with LightCycler® 480 System II (Roche Life Science, Germany). The HRM data was analyzed using the Melt Curve Genotyping and Gene Scanning Software (Roche Life Science ...
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bioRxiv - Neuroscience 2022Quote: The Förster Resonance Energy Transfer (FRET) has been performed in a LightCycler480 II (Roche, Canada) with variable excitation and detection filters ...
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bioRxiv - Cell Biology 2022Quote: ... cell viability was determined using the XTT cell proliferation kit II (Roche Diagnostics, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Cellular ATP levels were then determined using an ATP Bioluminescence Assay Kit HS II (Roche). Cells were diluted to OD600 = 0.1 with dilution buffer from the kit ...
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bioRxiv - Molecular Biology 2020Quote: ... RT-PCR was performed in duplicates using LightCycler® 480 II (Roche Life Science, Germany) and the average values of both the experiments were used for the analysis ...
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bioRxiv - Immunology 2020Quote: ... 0.8 mg/ml Dispase II and 0.1 mg/ml DNAse I (both Roche, Basel, Switzerland) at 37°C ...