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Citations for Roche :
851 - 900 of 4733 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... PCR amplification was carried out using the FastStart Essential DNA Green Master (Roche) on the QuantStudio 12K Flex (Applied Biosystems) ...
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bioRxiv - Immunology 2024Quote: ... Quantitative real-time PCR was performed using FastStart Universal SYBR Green master (Roche) and run on LightCycler 96 Systems (Roche) ...
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bioRxiv - Genetics 2020Quote: ... and 90 ng of each used for PCR using KAPA HiFi HotStart Ready Mix PCR kit (Kapa Biosystems) in 50 ul reactions ...
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bioRxiv - Genomics 2022Quote: ... the beads were used directly for PCR using a KAPA HiFi PCR ready mix (Roche KAPA Biosystems, Wilmington). The captured libraries from both PCR events were then combined and purified ...
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bioRxiv - Genomics 2022Quote: ... the beads were used directly for PCR using a KAPA HiFi PCR ready mix (Roche KAPA Biosystems, Wilmington). The captured libraries from both PCR events were then combined and purified ...
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bioRxiv - Microbiology 2024Quote: ... The lcrF transcripts of interest were detected with a DIG-labeled PCR fragment (DIG-PCR nucleotide mix, Roche) amplified with primer pairs for the individually detected according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Probes were generated using the PCR DIG labelling Mix (Roche, Mannheim, Germany) according to the manufacturer’s instructions (Table S1).
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bioRxiv - Microbiology 2019Quote: ... PCR amplification was performed using the KAPA2G Fast Multiplex Mix (Kapa Biosystems) and an equal molar amount of each forward primer and an excess of the universal reverse primer ...
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bioRxiv - Plant Biology 2020Quote: ... and amplified by PCR using KAPA HiFi Hotstart Ready Mix (KAPA Biosystems) with the same primers described above ...
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bioRxiv - Genetics 2020Quote: ... Probes were generated with the PCR DIG labeling Mix (Roche, Mannheim, Germany) following the manufacturer’s instructions and chemiluminescent signals were detected using the GelDocTM XR+ system (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... Cells were then PCR amplified in KAPA HiFi 2× mix (Kapa Biosystems) with barcoding primers ...
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bioRxiv - Biochemistry 2023Quote: ... by PCR (Table S2) using KAPA HiFi HotStart Ready Mix (Roche, 07958935001). Plasmid sequences were confirmed by Sanger sequencing.
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bioRxiv - Cell Biology 2023Quote: ... and PCR amplified using the KAPA HiFi HotStart ready mix (KAPA Biosystems) for 8 PCR cycles (45 s 98°C of initial denaturing and amplification at 15 s at 98°C ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA amplification was conducted with Kapa HiFi HotStart PCR mix (Kapa Biosystems) using PE1 and PE2 indexing primers (Supplementary Table S1) ...
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bioRxiv - Immunology 2024Quote: ... 1 U HiFi enzyme mix (Expand High Fidelity PCR System, Roche, USA) and 1.5 mM MgCl2 (Roche ...
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bioRxiv - Microbiology 2019Quote: ... Analysis was performed at IDEXX BioAnalytics (Columbia, MO, USA) with standard primer and probe concentrations using the master mix LightCycler® 480 Probes Master (Roche Applied Science, Indianapolis, IN, USA) in a commercially available instrument (LightCycler® 480 ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative real-time PCR (qRT-PCR) was performed with the LightCycler 480 SYBR Green I Master kit (Cat. 04887352001, Roche). Primers for pmp-3 and ACTIN were used as housekeeping for C ...
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bioRxiv - Neuroscience 2020Quote: ... and qPCR was performed with HOT FIREPol EvaGreen qPCR Mix Plus (Solis Biodyne) or qPCRBIO SyGreen Mix Lo-ROX (PCR Biosystems Ltd) on LightCycler 96 (Roche). The levels of CNOT4 mRNA expression were used for normalization ...
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bioRxiv - Biophysics 2020Quote: ... Beads in the sample and control fractions were then pulled down and the looping buffer was replaced with 50 μl of PCR mix (25 μl 2x HiFi KAPA Hot Start ready mix (Roche), 1 ul each of 100 μM primers (supplementary note 1) ...
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bioRxiv - Microbiology 2019Quote: ... 2 µl of 1:10 diluted DNA or cDNA and the SYBR Green I Master mix (Roche). We used PCR conditions adapted from Henneberger et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We used a Roche LightCycler 480 with the SYBR Green Master Mix (Roche Applied Science Indianapolis, IN). All samples were run with 3 technical replicates using 10 μL reactions ...
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bioRxiv - Genomics 2020Quote: ... Transcripts were quantified by using LightCycler® 480 SYBR Green I Master Mix (Roche, Cat. N: 04707516001) with the appropriate primers (see key resource table ...
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bioRxiv - Developmental Biology 2020Quote: ... qPCR was performed using a Roche LightCycler 480 with LightCycler 480 SYBR Green I Master Mix (Roche). Amplification efficiencies were calculated for each primer pair and minus template controls were included for each experiment ...
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bioRxiv - Cancer Biology 2019Quote: ... cDNA was used to perform qPCR using Light cycler 480 SYBR green I Master Mix from Roche Diagnostis (Catalog no ...
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bioRxiv - Molecular Biology 2020Quote: ... in a 20 µL reaction containing 10 µL LightCycler ® 480 SYBR Green I Master Mix (Roche) and 0.8 µL each of 10 µM forward and reverse primer listed in Supplementary Table S2 ...
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bioRxiv - Bioengineering 2020Quote: Quantitative Polymerase Chain Reactions (qPCR) were prepared in 10μ reactions with SYBR Green I Master Mix (Roche), 1μL of genomic extract as a template ...
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bioRxiv - Molecular Biology 2019Quote: ... The levels were normalized to two housekeeping genes (Actb, Gapdh) amplified with SYBR Green Master Mix (Roche), as reported before (22).
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bioRxiv - Cell Biology 2019Quote: ... in presence of 250 nM of specific primers with Kappa SYBR Fast qPCR Master Mix (Kapa Biosystems) on a CFX96 touch real-time PCR Detection System (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... qPCR was performed on a Roche LightCycler 480 with LightCycler 480 SYBR Green I Master Mix (Roche). Relative Expression Software Tool (REST ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RT-qPCR reactions were performed on 10 ng cDNA using LightCycler 480 SYBR Green Master Mix (Roche) with 10 ul reaction volumes ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR reaction was performed in a Roche Lightcycler using FastStart Essential DNA Probes Master mix (Roche) and gene specific primer/probe sets (Table 1 ...
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bioRxiv - Genomics 2022Quote: ... RT-qPCR was performed using a LightCycler 96 and the SYBR green master mix (Roche, Solna, Sweden). Gene expression levels were normalized to the AT5G09810 and AT5G25760 reference genes and displayed in relative units ...
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bioRxiv - Microbiology 2020Quote: ... 0.5 μM of each primer and 10 μl of Master Syber Green 1 mix (Roche, Basel, Switzerland). The Primer sequences listed in Table 1 ...
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bioRxiv - Plant Biology 2020Quote: ... RT-qPCR amplifications were performed from 10 ng of cDNA using SYBR Green I master mix (Roche) in 96-well plates according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2021Quote: ... The adapter-ligated gDNA was purified and amplified in 20 reactions using KAPA HiFi master mix (Roche) and Oligos 5 and 6 which add the homology flanks for the NEB HiFi DNA assembly ...
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bioRxiv - Plant Biology 2020Quote: ... The reaction was carried out on Roche Lightcyler® 480 instrument using SYBR Green Master Mix (Roche). The reaction system of real-time quantitative PCR was 10 μL ...
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bioRxiv - Cell Biology 2020Quote: ... RT-qPCR to assess for mRNA relative expression was performed with SYBR green master mix (Roche, 04913914001) in Applied biosciences machine ...
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bioRxiv - Neuroscience 2022Quote: ... R: 5’-GACCTGCAGGAGGATCGTAG −3’) was determined by qPCR using FastStart Essential DNA Green Master Mix (Roche, 06402712001) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... and qPCR analysis was carried out using specific primer pairs and SYBR green master mix (Kapa Biosystems) with a QuantStudio 5 Real-Time PCR System (Thermo Fisher) ...
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bioRxiv - Plant Biology 2022Quote: ... and C15 was conducted via qPCR using KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems, Roche, Switzerland) and 2 mL of 1:10 diluted cDNA in an Applied Biosystems Step One Plus real-time PCR instrument (ThermoFisher ...
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bioRxiv - Plant Biology 2022Quote: ... and C15 was conducted via qPCR using KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems, Roche, Switzerland) and 2 mL of 1:10 diluted cDNA in an Applied Biosystems Step One Plus real-time PCR instrument (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... qPCR reactions were performed using the KAPA SYBR® FAST qPCR Master Mix (2X) Kit (Kapa Biosystems) on an Agilent Stratagene Mx3000P Multiplex Quantitative PCR System under the following conditions ...
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bioRxiv - Genomics 2022Quote: ... 100 ng of RNA was used for reverse transcription (RT) using Transcriptor universal cDNA master mix (Roche) for Min6 cells or superscript III enzyme (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... The cDNA was mixed with the primers of interest and with SYBR Green Master mix (Roche #04887352001) using the Bravo instrument (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... reactions were set up at 10 µl reaction volume using LightCycler 480 SYBR Green Master Mix (Roche) and performed using the LightCycler 480 Real-Time PCR Instrument (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... qPCR followed the Lightcycler 480 SYBR Green I Master mix product manual for 384 multiwell plates (Roche). All primers are listed in Supplemental Table S7 ...
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bioRxiv - Developmental Biology 2024Quote: ... qPCR reactions were set up in technical duplicates using LightCycler 480 SYBR Green I Master Mix (Roche), 5 ng amplified DNA and 1 μM forward and reverse primers and run on a LightCycler 480 machine (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time PCR was conducted using LightCycler® 480 SYBR Green I Master (Roche) on a LightCycler® 480 System (Roche) ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative PCR was performed using LightCycler® 480 SYBR® Green I Master (Roche) using the following gene specific primers ...
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bioRxiv - Microbiology 2020Quote: ... DENV-3 RNA was amplified by qRT-PCR (LightCycler Multiplex RNA Virus Master, Roche), using primers to the conserved 3’UTR ...