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Citations for Roche :
8751 - 8781 of 8781 citations for 6H Imidazo 4 5 e 2 1 3 benzothiadiazole 7 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Cells were lysed in Tris-Buffered Saline (TBS) + 1% Triton X-100 with EDTA and protease and phosphatase inhibitor cocktails (Roche Diagnostics, Florham Park, NJ). To remove insoluble materials ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 3 μL of each primer at 10 μM and 0.5 μL of 1 U/μL Kapa HiFi HotStart DNA polymerase (Kapa Biosystems, Inc, A Roche Company) for a total volume of 25 μL ...
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bioRxiv - Biophysics 2024Quote: ... to a final molal concentration of 25 ng/μL and amplified using primers specific to each oligonucleotide subpool with KAPA SYBR FAST qPCR (KAPA Biosystems; 1-ng template). A second PCR amplification was performed with KAPA HiFi (KAPA Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclear pellet was rinsed with 500 µL of resuspension buffer (20 mM Tris pH 8, 75 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.125 mM PMSF, 50 % glycerol, 1x Roche complete, 20 U/mL RNase Out) and centrifuged at 4000 xg at 4°C for 3 min ...
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bioRxiv - Microbiology 2023Quote: ... salinarum were generated by isothermal Gibson assembly (Gibson, 2011) of: 1) the PCR fragments (amplified by KAPA Biosystems DNA polymerase [VWR] and gel extracted); and 2 ...
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bioRxiv - Developmental Biology 2022Quote: ... against foxg1 was carried out as previously described by Thisse (Thisse and Thisse, 2008) The antibody used to detect the riboprobes was AP-anti-DIG (Roche Cat.N: 11093274910, 1:500). For Fluorescent WM ISH (WM FISH) ...
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bioRxiv - Biochemistry 2023Quote: ... in presence of MBP (10 μg) in the kinase assay buffer (50 mM Tris, 50 mM MgCl2, 1× phosphatase inhibitor cocktail (Roche Life Science, Indianapolis, IN), 1 mM DTT ...
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bioRxiv - Cancer Biology 2023Quote: ... Frozen bone marrow cells from MPN patients or frozen leukopaks from healthy donors were thawed and incubated overnight in RPMI media with DNase 1 (Roche 10104159001, 0.1 mg/mL). Freshly collected bone marrow samples from some MPN patients were used directly for cell isolation ...
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bioRxiv - Biochemistry 2023Quote: ... 100 ng of recombinant PfCDPK1 protein was taken in a kinase assay buffer (50 mM Tris, 50 mM MgCl2, 1× phosphatase inhibitor cocktail (Roche Life Science, Indianapolis, IN), 1 mM DTT ...
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in non-denaturing buffer at 4°C (10 mM Tris, 500 mM NaCl, MgCl2 1mM, 3U of DNase I, and protease inhibitor cocktail 1 X from Roche, ref 4693116001, pH 7.9) followed by sonication and ultracentrifugation at 13000 rpm for 45 min at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... 1/100 cDNA dilutions were analysed using a LightCycler®480 and LightCycler®480 SYBR Green I Master (Roche, Welwyn Garden City, UK) following manufacturer’s instructions and cycling conditions of pre-incubation 95°C for 5 min ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections as previously described (46) (Iba-1 staining was conducted on a Ventana Benchmark using OptiView and UltraView detection kits provided by Roche (Roche Molecular Systems, Inc). Sections were deparaffinized in xylene and rehydrated through an ethanol series ending in distilled water ...
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bioRxiv - Neuroscience 2023Quote: DRGs were collected from both WT and KO mice (8 weeks male) and were incubated in 1 mg/ml Collagenase/Dispase (Roche Diagnostics, Madison, WI, USA) at 37℃ for 60 min ...
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bioRxiv - Cell Biology 2023Quote: ... Sectioned zebrafish muscle tissue was rinsed in phosphate buffered saline (PBS) for 15 min before the addition of blocking solution (1% blocking reagent (Roche Diagnostics GmBH, Manheim, Germany) with 0.4% Triton X ...
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bioRxiv - Cell Biology 2022Quote: ... and lysed in ice-cold lysis buffer (20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 10% Glycerol, 1% Triton X-100, cOmpleteTM protease inhibitor cocktail tablet from Roche and a PhosSTOP tablet from Roche). Lysates containing 500-1000 μg protein (made up to 500 μl by lysis buffer ...
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bioRxiv - Immunology 2021Quote: Single cells were sorted directly into PCR tubes containing 25 μl PCR lysis buffer (1x Buffer 1 from the Expand Long Template PCR System [Roche], 0.5 mg/ml Proteinase K) and lysed for 1h at 55°C ...
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bioRxiv - Systems Biology 2019Quote: ... Single cells were collected in 1 μL of cell lysis buffer (1 U RNasein plus [Promega, Madison, WI], 10% RealTime ready Cell Lysis Buffer [Cat# 06366821001, Roche], 0.3% NP40 (Thermo Fisher Scientific), and RNase-free water (TaKaRa ...
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bioRxiv - Neuroscience 2020Quote: Sections were treated with 2% H2O2 in PBS to inactivate endogenous peroxidase followed by incubation with anti-DIG antibodies POD fragments in combination with anti-DNP POD (Roche, Basel Switzerland; dilution 1:1000). Incubations with these antibodies were done separately at 4°C ...
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bioRxiv - Cell Biology 2022Quote: A teratoma was cut into smallest possible pieces and dissociated for 1 hour at +37C in a collagenase A solution (Roche, 0.1 mg/ml in DMEM). The resulting cell suspension was passed through mesh strainers with pore diameter from 100 μm to 40 μm ...
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bioRxiv - Neuroscience 2023Quote: ... Cultured cells were harvested at different time points as indicated in the figures and lysed in 1% Triton X-100 buffer containing protease inhibitor cocktail (Roche diagnostics, # 11-836-170-001), 1 mM PMSF ...
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bioRxiv - Plant Biology 2023Quote: First-strand cDNA was synthesized by reverse transcribing 1 μg of total RNA in a final reaction volume of 20 μl using MMLV reverse transcriptase (Roche High Fidelity cDNA synthesis kit) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... At the end of the cell disruption dry homogenised powder of cells were resuspended in 1 ml urea buffer (8 M urea, 50 mM Tris/HCl pH 8.0 containing one tablet of ‘cOmplete’ protease inhibitor per 25 ml, Roche, Germany; 1 mM DTT; 0.1 M PMSF) and centrifuged at 16,000 × g for 10 minutes at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... using the primers indicated in Table 1 after cDNA synthesis using random hexamer primers and Roche AMV reverse transcriptase (10109118001 Roche, Sigma-Aldrich, St. Louis, MO, USA). Samples were multiplexed using Takara long amplicon Taq polymerase with GC buffers following the manufacturer’s instructions for PCR barcoding for nanopore sequencing (EXP-PBC096 Oxford Nanopore Technologies ...
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bioRxiv - Pathology 2021Quote: ... Tissue sections were then blocked with blocking solution buffer II (containing 0.1% Triton X-100 and 2% normal sheep serum) at room temperature for 30 min before covering with anti-DIG alkaline phosphatase conjugate anti-digoxigenin antibody (Roche, diluted 1:500 in buffer II) for 1 h at 45°C ...
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bioRxiv - Genetics 2019Quote: ... Skin fibroblasts from three controls and the affected individual were recovered in ice cold RIPA buffer with protease inhibitors (‘Complete EDTA-free’; Roche Diagnostics, 1 tablet in 10ml buffer) and 25mM N-ethylmaleimide (NEM ...
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bioRxiv - Immunology 2022Quote: ... All buffers were kept on ice and the final Cell Lysate Buffer was supplemented with cOmplete™ Mini EDTA-free Protease Inhibitor Cocktail (#11836170001, Roche, 1 tablet per 25 ml) and PhosSTOP™ (#4906845001 ...
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bioRxiv - Molecular Biology 2023Quote: ... Each cell pellet was resuspended in 35 mL of lysis buffer (50 mM Na-phosphate buffer pH 8.0, 500 mM NaCl, 14 mM β-mercaptoethanol, protease inhibitors [1 Roche Mini EDTA-free tablet/10 mL]). Cells were disrupted by sonication (4 times for 1 min with intermittent cooling on ice) ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mM EGTA, 20 mM MgCl2, 500 mM NaCl, 0.5% NP-40, protease inhibitor cocktail [Roche], and PhosStop [Roche] containing 20 μg of GST-Rhotekin-RBD). The lysates were centrifuged at 20,000 ×g for 3 min at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... HA detection was performed with a directly coupled α-HA antibody (α-HA-HRP, high-affinity monoclonal rat antibody, 3F10, Roche, catalog no. 12013819001, 1:1000). Immunodetection was performed after three washes with TBST for 10 min each ...
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bioRxiv - Cell Biology 2024Quote: ... Worms were resuspended in an equal volume of 2x lysis buffer (2x TBS, 0.2% TX-100, and 10% glycerol with protease inhibitors (Roche, 1 tablet per 25 ml lysis buffer)) ...
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bioRxiv - Cell Biology 2021Quote: ... The pellet was washed in buffer A three times and then homogenized in 400 ul of buffer B (250 mM sucrose, 10 mM Tris HCl [pH 8.0], 10 mM MgCl2, 1 mM EGTA, 1X protease-inhibitor cocktail [11697498001, Roche], 0.1% Triton X-100, and 0.25% NP-40) for 10 min at 4°C ...