Labshake search
Citations for Roche :
8351 - 8400 of 8781 citations for 6H Imidazo 4 5 e 2 1 3 benzothiadiazole 7 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2023Quote: RNA sequencing (RNAseq) libraries were prepared using 1 µg of total RNA using the KAPA stranded mRNA-seq kit (Roche) according to the manufacturer’s specifications ...
-
bioRxiv - Plant Biology 2023Quote: ... the AIRs were treated with 1 unit per AIR mg of type I α-amylase (Roche 10102814001, Roche GmbH, Germany). The de-starched AIRs ...
-
bioRxiv - Genetics 2023Quote: To extract genomic DNA cells were resuspended in genomic lysis buffer (300 mM NaCl, 1% SDS, 20 mM EDTA) and incubated with Proteinase K (Roche) at 55 °C overnight ...
-
bioRxiv - Biochemistry 2023Quote: ... CVF samples were normalized to a protein concentration of 1 mg/mL using sterile PBS and EDTA-free protease inhibitor cocktail (Roche) was added ...
-
bioRxiv - Physiology 2023Quote: ... in PEB Buffer (150 mM NaCl, 50 mM Tris-HCl, 5mM EDTA, 1% NP-40) containing proteases (cOmplete™, Roche) and phosphatase (Phosphatase Inhibitor Cocktail 2 ...
-
bioRxiv - Plant Biology 2023Quote: ... After nuclei were resuspended in RIPA buffer (1x PBS, 1% IGEPAL CA-630, 0.5% Sodium deoxycholate, 0.1 % SDS, Roche Complete tables) and the DNA was fragmented to 200-500 bp by sonication ...
-
bioRxiv - Cell Biology 2023Quote: Cells were plated in 96-well plates and treated as indicated for 72 hours before performing MTT survival assay using Cell Proliferation Kit 1 MTT (Roche) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... were homogenized in lysis buffer (PBS containing 0.3% Triton X-100, 1 mM sodium orthovanadate and protease inhibitors (Roche, 4693132001). Total protein of each sample was determined using the Bio-Rad DC kit (Bio-Rad ...
-
bioRxiv - Molecular Biology 2023Quote: ... Frozen cell pellets were resuspended in 300 μL of cold hypotonic buffer (10 mM Tris-HCl pH 8, 1.5 mM MgCl2, 1 mM KCl, 10X PhosphoSTOP (Roche, 4906845001), 2X Protease Inhibitor Cocktail (Roche ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Slides were incubated with 50 μL of block buffer containing a fluorescein-labeled anti-DIG antibody (sheep, 1:100, Roche) for 60 min at 37°C ...
-
bioRxiv - Neuroscience 2023Quote: ... Screening for repeat expansions in RFC1 was achieved by a combination of short-range repeat-spanning end-point PCR (Supplementary Table 1) with 2X Faststart PCR mastermix (Roche) and by Repeat-Primed PCR using 2X Phusion Flash High-Fidelity PCR Mastermix (Thermo Fisher) ...
-
bioRxiv - Genomics 2023Quote: ... resuspended in 30 ml of Solution A supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) and 0.175x cOmplete™ (Mini, EDTA-free) protease inhibitor cocktail (Roche) in a 50 ml Falcon tube and disrupted by vortexing with an equal volume of glass beads (425-600 μm ...
-
ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... A total of ∼1×107 HEK293T cells expressing GFP-STARD3 were lysed with 1 mL lysis buffer supplemented with a protease inhibitor cocktail (cOmplete, Roche). Lysates were clarified by centrifugation at 12000 g for 60 min and were mixed with the beads coated with 2 mg GST/GST-VAPA-MSP for 60 min at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... and lysed on ice using a lysis buffer (PBS, 0.1% SDS, 1% Triton X-100, EDTA-free complete protease inhibitor (Roche Diagnostics)) ...
-
bioRxiv - Cell Biology 2023Quote: ... IPTG-treated bacteria were washed with PBS and eluted with 1X HEPES buffer containing 1% Triton X-100 and EDTA-free protease inhibitor cocktail (Roche) The elutes were sonicated and centrifuged at 18,000 x g ...
-
bioRxiv - Synthetic Biology 2023Quote: ... cell pellets were resuspended in 10 ml cold TES buffer (50 mM Tris pH 8.0, 20% Sucrose, 1 mM EDTA) supplemented with EDTA-free complete protease inhibitor (Roche, Switzerland) and incubated at 4 °C for 45 mins ...
-
bioRxiv - Developmental Biology 2023Quote: ... followed by quick wash with PBS and lysis with 400 µM of lysis buffer (50LJmM Tris pH7.5, 150LJmM NaCl, 1% Triton-X supplemented with 2x protease inhibitor cocktail (Roche, 11836145001), 1x phosphatase inhibitor cocktail (Roche ...
-
bioRxiv - Developmental Biology 2023Quote: Five whole embryos or ten explants were lysed in 1% NP-40 supplemented with protease inhibitors (Complete Mini, EDTA-free tablet (Roche), Leupeptin (Roche) ...
-
bioRxiv - Microbiology 2023Quote: ... washed and lysed in a protein extraction buffer (10mM TrisHCl pH7.5, 5mM EDTA, 150mM NaCl, 30mM Na2HPO4, 50mM NaF, 10% glycerol, 1% NP40, 1X cOmplete (Roche #11873580001), 1X PhosSTOP (Roche #4906845001) ...
-
bioRxiv - Neuroscience 2023Quote: ... 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, protease inhibitor cocktail [Roche], and PhosStop [Roche]; pH 7.5) and sonicated for 30 s ...
-
bioRxiv - Pathology 2023Quote: ... The preparations were washed with buffer 1 and incubated for 2h at 37°C with an anti-digoxigenin antibody conjugated to alkaline phosphatase (1:200 in blocking solution; 11093274910, Roche). Next ...
-
bioRxiv - Neuroscience 2023Quote: Neurons were lysed with 200 μl SDS lysis buffer (1% SDS with protease inhibitor cocktail tablet; Roche, cat. no 04693159001) per well in 6-well plates and scraping ...
-
bioRxiv - Cell Biology 2023Quote: ... and the cell pellet was resuspended in 1 ml vPBS (PBS, 46 mM sucrose, 10 mM glucose) containing EDTA-free protease inhibitors (Roche). The washed cells were pelleted by centrifugation (750xg ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were lysed for 30 min on ice in lysis buffer supplemented with fresh 1 x protease inhibitor cocktail (Roche). Unless otherwise indicated ...
-
bioRxiv - Immunology 2023Quote: ... lung and liver tissues were mechanically disrupted into small pieces and enzymatically digested in 2ml of digestion buffer (1 mg Collagenase D (Roche) and 0.2 mg DNase I (Roche ...
-
bioRxiv - Genomics 2023Quote: ... The membrane was then extensively washed with low and high stringency buffers and subsequently blocked with buffer B2 (1% Blocking powder [Roche] in buffer B1 [100 mM Maleic acid ...
-
bioRxiv - Microbiology 2023Quote: ... and the murine J774A.1 (ATCC TIB-67) macrophages was assessed using a lactate dehydrogenase (LDH) cytotoxicity detection kitPLUS (Roche), which measures the release of the LDH enzyme in the growth medium ...
-
bioRxiv - Microbiology 2023Quote: ... Nuclear pellets were resuspended in shearing buffer (0.1% SDS, 1 mM EDTA, 10 mM Tris-HCl pH 7.5, 1X Roche protease inhibitor, and 1X Roche phosphatase inhibitor) and transferred into 130 μl Covaris tubes (Covaris ...
-
bioRxiv - Microbiology 2023Quote: ... cells were washed with 1x PBS then lysed in 1% Triton lysis buffer or 1x RIPA lysis buffer supplemented with Complete Mini Protease inhibitor cocktail (Roche). The concentration of protein was then assessed via BCA and samples were normalized and added to sample buffer supplemented with 10% BME before being run on an SDS-PAGE gel (Invitrogen NW04125BOX) ...
-
bioRxiv - Cell Biology 2023Quote: ... The membrane was blocked with freshly prepared blocking solution (100 mM maleic acid, 150 mM NaCl, pH 7.5, 1% (wt/vol) blocking reagent (Roche, 11096176001)) for 30 min ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were re-suspended in 500 µL of lysis buffer (PBS containing 1 % Triton-X 100 and complete protease inhibitors [Roche]). DAPI was added to lysis buffer (final concentration 5 µM ...
-
bioRxiv - Cell Biology 2023Quote: ... First-strand cDNAs were obtained by reverse transcription of 1 μg of total RNA using the High-Capacity cDNA Reverse Transcription Kit (Roche), according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: RPE-1 cells were trans-fected with the different Flag constructs for 48 h using X-tremeGENE 9 DNA reagent (Roche). Cells were lysed in 25mM Tris (pH 7.5) ...
-
bioRxiv - Genetics 2024Quote: The entire ∼8kb NR2E3 locus was amplified using NR2E3 F and NR2E3 R primers (Table 1) with the Expand Long Template PCR System (Roche) buffer system 1 ...
-
bioRxiv - Immunology 2024Quote: ... the tissue samples were dissected into smaller sections with sterile scalpels and incubated in 100 μl of PBS and 50 μl of Liberase TL (1 mg/ml; Roche) for one hour at +37°C ...
-
bioRxiv - Neuroscience 2024Quote: ... life-Tein LLC) was mixed with 50µl of PBS containing 1% Tween20 and 5ng/mL anti-HA-Peroxidase 3F10 antibody (Roche #12013819001) and transferred to the wells ...
-
bioRxiv - Cancer Biology 2024Quote: ... desoxycholic acid-Na-salt 1%, SDS 0.1%) (Serva electrophoresis, Germany) containing protease inhibitor cocktail and phosphatase inhibitor cocktail (Roche, Switzerland). Western blots were performed following routine protocols67 ...
-
bioRxiv - Molecular Biology 2024Quote: ... of RIPA buffer (150 mM NaCl, 50 mM Tris pH 8.0, 1% NP40, 0.5% Na-deoxycholate, 0.1% SDS and Roche complete protease inhibitors) using a Teflon homogenizer ...
-
bioRxiv - Bioengineering 2024Quote: ... or 1 M NaCl (for ori designs), 20 µg/mL DNase (#A3778, ITW Reagents) and protease inhibitor cocktail (04693132001, Roche)) ...
-
bioRxiv - Neuroscience 2024Quote: ... The mixture for qPCR reaction was prepared in a final volume of 20 μl containing 1 μl cDNAs and 10 μl of LightCycler 480 SYBR Green I Master (Roche) in the presence of primers at 500 nM ...
-
bioRxiv - Neuroscience 2024Quote: ... Larvae were blocked and then incubated with 1/4000 concentration of anti-DIG Fab fragment conjugated with alkaline phosphatase (Roche) overnight at 4ºC ...
-
bioRxiv - Plant Biology 2024Quote: Seed histological sections were washed with Phosphate Buffered Saline solution (PBS) and digested with 1 mg/ml proteinase-K (Hoffmann-La Roche). Subsequently ...
-
bioRxiv - Biophysics 2024Quote: ... 2.0 × 106 cells/mL of WT Neuro2a or OptoTau KI cells were incubated in 10 mM of HEPES buffer supplemented with 1% protease inhibitor (cOmplete, Roche), 1 µM of okadaic acid (Cayman Chemical) ...
-
bioRxiv - Cancer Biology 2024Quote: Xenograft OS tumors were harvested and microdissected 12 d after 143B OS cell inoculation and digested with collagenase type I and II (1 mg/ml; Roche) digestion for 45 min ...
-
bioRxiv - Neuroscience 2024Quote: ... Slides were washed 3x 15 minutes in room temperature TBST (Tris-buffered saline with 1% Tween 20) and blocked at room temperature for one hour in 10% DIG buffer (Roche) in TBST.
-
bioRxiv - Cancer Biology 2024Quote: ... Total proteins from T-ALL#1 cells were extracted with a RIPA buffer containing a cocktail of 1Xprotease inhibitors (11836145001, Roche) and 1X phosphatase inhibitors (P2850 ...
-
bioRxiv - Immunology 2024Quote: ... Quantitative RT-PCR analysis was performed with selective primers (Supplementary Table 1) using the Light Cycler 480 SYBR Green I Master X2 Kit (Roche), according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2024Quote: ... Probed membranes were washed and incubated with horseradish peroxidase-conjugated anti-mouse or anti-rabbit Abs for 1 hour and developed with Lumi-LightPLUS Western Blotting Substrate (Roche). Quantification was performed on Lumi-Light autoradiography films using ImageJ software.
-
bioRxiv - Immunology 2024Quote: ... whole pituitaries or separate posterior and anterior pituitaries were mechanically lysed by suspending and digested with 50 µg/ml DNase 1 (Roche) and 1 mg/ml Collagenase D (Roche ...
-
bioRxiv - Molecular Biology 2024Quote: ... supplemented with a 20-fold excess of unlabeled oligonucleotide with the same sequence as the labeled one (to prevent reannealing) and 1 µl of Proteinase K (18 mg/ml) (Roche). After deproteination for 30 min at 37 °C ...