Labshake search
Citations for Roche :
8601 - 8650 of 8748 citations for 7 Chloro 1 3 dihydro 5 phenyl 2H 1 4 benzodiazepin 2 thione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... coli cell pellets was carried out by resuspending pellets in 50 mL of lysis buffer (50 mM NaPO4, 300 mM NaCl, 20 mM imidazole, 10 mM 2-mercapoethanol, protease inhibitor (Roche), DNAse (Sigma) ...
-
bioRxiv - Cell Biology 2023Quote: ... 4 ml of HBSS (without Ca2+ or Mg+) and 10 ml of collagenase solution [2 mg/ml collagenase H (11074059001; Roche), 0.1 mg/ml DNaseI (10104159001 ...
-
bioRxiv - Immunology 2023Quote: ... Tumors excised at day indicated in each figure were disaggregated mechanically and enzymatically with 2 mg/mL collagenase IV and 50 U/mL DNase I (Roche).
-
bioRxiv - Cell Biology 2022Quote: ... Cells were then resuspended in 4x pellet volume of sucrose buffer (10 mM HEPES/KOH pH 7.5.; 2 mM MgAc; 250 mM Sucrose, 1x Protease inhibitor cocktail [Roche, Germany]) and lysed with ~50 strokes in a tight-fit dounce homogenizer ...
-
bioRxiv - Immunology 2023Quote: ... Cells were lysed in lysis buffer (2% SDS in 50 uM Tris-HCL pH.8, supplemented with EDTA-free protease inhibitor, Roche) at cell concentration of 20×106/mL and incubated at 95 °C for 15 minutes ...
-
bioRxiv - Genomics 2022Quote: ... Re-purified circular fragments were amplified by PCR with View-Point specific primers (see Supplementary Table 2) using the Expand Long Template PCR System (Roche). Resulting amplicons were purified with a 0.8× ratio of AMPure XP beads (Beckman Coulter ...
-
bioRxiv - Biochemistry 2023Quote: ... the cell pellets resuspended in lysis buffer (purification buffer 50 mM Tris pH 8, 50 mM NaCl, 2 mM DTT; + cOmplete EDTA-free Protease Inhibitor (Roche)) and lysed by sonication ...
-
bioRxiv - Microbiology 2023Quote: ... The suspension medium was carefully removed and replaced with fresh PDB supplemented with 2× protease inhibitor (Mini Protease Inhibitor Cocktail, Roche), 0.5 mM or 2 mM PMSF (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2024Quote: ... for all qPCRs except C9orf72 variant 2 expression levels for which TaqMan probes were used (sequences provided in Table S2) and measured with a LightCyclerTM (Roche). qPCR was performed in technical triplicate with data normalised to GAPDH expression levels.
-
bioRxiv - Microbiology 2023Quote: ... containing 5 mL dissociation buffer (RPMI-1640 (Cytiva) with 25 mM HEPES and 10 µg/mL gentamicin sulfate) supplemented with 2 mg/mL Collagenase-D (Roche) and 100 U DNaseI (Roche) ...
-
bioRxiv - Neuroscience 2023Quote: Hippocampi were homogenized in 500µl of RIPA medium lysis buffer (Beyotime, #P0013E-2) in the presence of protease inhibitor cocktail (Roche, #11836170001). Samples were centrifuged at 10,000 × g for 30 minutes ...
-
bioRxiv - Biophysics 2023Quote: Cell lysate powder from 12 L of cell culture was resuspended in 300 mL of room temperature eIF4G Lysis Buffer supplemented with 2 EDTA-free protease inhibitor tablets (Roche), 20mM imidazole ...
-
bioRxiv - Molecular Biology 2024Quote: ... a total of 4.5–6 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). The second transfection was performed 3 days after the first transfection and the cells were harvested after an additional 5 days ...
-
bioRxiv - Biochemistry 2024Quote: ... For lysis the pellets are thawed and resuspended in 2 ml of lysis buffer containing benzonase and cOmplete protease inhibitors (Roche), and processed as described for small scale expression in E ...
-
bioRxiv - Systems Biology 2024Quote: The eluted samples were used as a template for a PCR reaction for amplifying Tn5 tagmented fragments and sample barcoding (2× Kappa HIFI [Roche] ...
-
bioRxiv - Molecular Biology 2024Quote: ... a total of 3–5 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). Transfection was repeated 2 days and 5 days after the first transfection and the cells were harvested after an additional 4 days ...
-
bioRxiv - Immunology 2020Quote: ... and a 30-gauge needle was used to inflate each pancreas through the common bile duct with 4 mL of media supplemented with 0.8 mg/mL of collagenase P (Roche, cat. number 11-249-002-001) and filtered at 0.22 μm prior to injection ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... qPCR mix was prepared in a total volume of 10 μL with 5 μL of LightCycler® 480 SYBR Green I Master (Roche, Basel, Switzerland), 4 μL of diluted cDNA (or water for the negative control ...
-
bioRxiv - Microbiology 2022Quote: ... The 10 μL reaction mixture included 5 μL Kapa SYBR Fast Universal 2x qPCR master mix (Kapa Biosystems, Lab Supplies Scientific SA, Hellas), 5 ng of cDNA template ...
-
bioRxiv - Microbiology 2022Quote: ... Approximately 800 μg of VLP lysate was then reduced and alkylated as described above and treated with 5 U PNGase F (Roche Cat. Nr. 11365177001) at 37 °C for 12 hours followed by 12 hours with 11 μg of trypsin ...
-
bioRxiv - Microbiology 2021Quote: ... and Fwd-P2 (5′-CCATCTCATCCCTGCGTGTCTCCGACTCAGBBBBBBAGARTTTGATCYTGGTTCAG and the reverse primer was Rev1B (5′-CCTATCCCCTGTGTGCCTTGGCAGTCTCAG. The PCR products were sequenced on the 454 GS FLX Titanium sequencer (Hoffmann-La Roche, LTD; Basel, Switzerland). Through the Research Alliance for Microbiome Science Registry (IRB no ...
-
bioRxiv - Microbiology 2019Quote: ... where N5 represents 5 random bases used to improve sequencing quality) using 25 µL of Kapa Hifi mastermix (Kapa Biosystems, Woburn, MA, USA), 2.5 µl of each primer (10 µM) ...
-
bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT, 150 mM NaCl, 5 mM MgCl2, 0.001% Triton X-100, 1mM PMSF, 0.2 mM GDP, Roche cOmplete EDTA-free protease inhibitors) and lysed with a microfluidizer ...
-
bioRxiv - Plant Biology 2022Quote: ... and then mixed with 4ml protein extraction buffer (50mM Tris/HCl pH 7.5, 5% glycerol, 150mM NaCl, 0.2% triton X-100, cOmplete™ EDTA-free protease inhibitor tablets [Roche] - one per 10ml buffer) as soon as the ground tissue had reached room temperature ...
-
bioRxiv - Plant Biology 2022Quote: ... protein extraction buffer (50mM Tris/HCl pH 7.5 or HEPES pH 7.5, 150mM NaCl, 5% glycerol, 0.5% NP-40, cOmplete™ EDTA-free protease inhibitor tablets [Roche] - one per 10ml buffer) was added to the tissue and grinding was continued until a homogenous suspension was formed ...
-
bioRxiv - Biochemistry 2023Quote: ... and collected by centrifugation at 7,500 RCF for 15 minutes and then suspended in Lysis Buffer D (20 mM HEPES pH 8, 200 mM NaCl, 5 mM imidazole, 0.5 mM Imidazole, 1x Roche EDTA free protease inhibitor). Cells were lysed using an emulsifier (AvestinEmulsiflexC3 ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were suspended in Resuspension Buffer A (20 mM HEPES pH 8, 150 mM NaCl, 5% glycerol, 0.5 mM TCEP, Roche EDTA free protease inhibitor) and lysed using an emulsifier (AvestinEmulsiflexC3) ...
-
bioRxiv - Cancer Biology 2021Quote: ... and estrous cycle phase were pooled in PBS and minced followed by enzymatic digestion in DMEM/F12 media containing 2 mg/ml Collagenase A (Roche #11088793001) and 100 U/ml Hyaluronidase (Sigma #H3506 ...
-
bioRxiv - Immunology 2021Quote: ... Size selection using SPRI-beads isolated low molecular weight DNA which was then PCR amplified using 2 × HiFi HotStart Ready mix (Roche Diagnostics) and Nextera Indexing Primers (Illumina ...
-
bioRxiv - Immunology 2021Quote: ... PBMC were seeded at 1 × 106 cells/mL in erythroid differentiation-promoting medium based on StemSpan™ Serum-Free Expansion Medium (SFEM) supplemented with human recombinant EPO (2 U/ml, Roche), human recombinant stem cell factor (25 ng/ml ...
-
bioRxiv - Biochemistry 2022Quote: ... and 0.1 mM EDTA) supplemented with 10 mM 2-mercaptoethanol (BME) and four EDTA-free cOmplete protease inhibitor tablets (Roche Applied Science) prior to lysis using the Nano DeBEE High Pressure Homogenizer (BEE International) ...
-
bioRxiv - Developmental Biology 2019Quote: ... washed in HM, resuspended in lysis buffer (SDS 2%, Tris-HCl pH 8.0 100 mM containing a mix of protease (Roche complete cocktail) and phosphatase inhibitors (Biotools cocktail or lab-made cocktail ...
-
bioRxiv - Molecular Biology 2020Quote: ... cDNA was diluted 1:2 (20 µL reverse transcription reaction to 40 µL water) and 2 µL of diluted cDNA was amplified in the LightCycler ® 480 Instrument II (Roche) in a 20 µL reaction containing 10 µL LightCycler ® 480 SYBR Green I Master Mix (Roche ...
-
bioRxiv - Immunology 2019Quote: ... containing recombinant IL-2 (obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH from Dr. Maurice Gately, Hoffmann - La Roche Inc61), to give a final IL-2 concentration of 100 units/mL ...
-
bioRxiv - Immunology 2019Quote: ... the HEK cells were lysed with a non-denaturing lysis buffer (20 mM Tris, pH 8.0, 137 mM NaCl, and 2 mM EDTA) supplemented with a protease inhibitor (Roche, Basel, Switzerland). After 10 min of incubation on ice ...
-
bioRxiv - Immunology 2021Quote: ... variable region) was used as template in a 25 µl PCR reaction with 2× KAPA HiFi Hot Start Ready Mix (Roche #KK2602) and 1 µl (10 µM ...
-
bioRxiv - Immunology 2021Quote: ... cells were maintained in media containing 10 IU/ml rhIL-2 (AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH from Dr. Maurice Gately, Hoffmann - La Roche Inc). Day 6-9 post-activation ...
-
bioRxiv - Microbiology 2021Quote: ... mgc2 adhesin and mgc2-like adhesin (list of primers used in Table 2) were made by real-time PCR (rtPCR) on LightCycler 96 thermocycler (Roche®). The LightCycler SYBR Green I Master reaction mix (Roche® ...
-
bioRxiv - Cancer Biology 2020Quote: ... Recombinant MYC was eluted from the beads using SUMO elution buffer (250 mM imidazole, 10% glycerol, 1X PBS, 2 mM DTT) + PIC (Roche 05056489001).
-
bioRxiv - Cell Biology 2021Quote: ... lysed by sonication in ice-cold Tris buffer (50 mM Tris pH 8.0, 150 mM NaCl, 10 mM imidazole, 2 mM βME, complete protease inhibitors (Roche, Basel, Switzerland), 1 μg/ml DNase ...
-
bioRxiv - Genomics 2020Quote: ... Note that all other characteristics observed for a retrotransposon insertion when using the exome capture kit from Roche (see Figure 2) are also observed when using the kit from Agilent.
-
bioRxiv - Cell Biology 2022Quote: ... Purified and digested DNA was ligated to PCR products at 16°C overnight in a final volume of 20 μl using 2 U of T4 DNA ligase and 1x ligation buffer (Roche, 10481220001). Plasmid DNA was transformed into TOP10 chemically competent cells (Invitrogen ...
-
bioRxiv - Developmental Biology 2022Quote: ... pellets were dissolved with 300 µl 1X PMPG buffer (1x PBS no Mg2+/Ca2+, 2% Empigen) containing protease inhibitors (Roche, 11873580001) and RNasin (Promega) ...
-
bioRxiv - Cell Biology 2022Quote: ... Medium calcium content was measured by photometrical absorption measurement of calcium EDTA complex using a Calcium Gen.2 kit (Roche, Germany). After 7 days ...
-
bioRxiv - Neuroscience 2021Quote: ... the L4/5 DRG were rapidly harvested from the isoflurane anesthetized naïve rats and were incubated in 0.01% blendzyme 2 (Roche Diagnostics, Madison, WI) for 30 min ...
-
bioRxiv - Cell Biology 2021Quote: TUNEL staining including antigen retrieval (microwave 5min x 2) and staining process was performed following manufacturer’s instruction (11864795910, Roche Applied Science). FITC-labeled TUNEL-positive cells were imaged under fluorescent microscopy ...
-
bioRxiv - Microbiology 2022Quote: ... Immunoreactivity in the lung tissues was assessed using a rabbit anti-SARS-CoV-2 Nucleocapsid pAb as the primary antibody (Sinobiological, China) and further processed using a Ventana Discovery Ultra (Roche, USA) system.
-
bioRxiv - Molecular Biology 2019Quote: ... Dehydrated slides were then placed on coverslips containing 70 µL hybridization mix (10 mM Tris-HCl, 2% blocking reagent (Roche, #11096176001), 70% formamide and 0.1 µM Cy3 labeled (CCCTAA)3 PNA probe (PNA Bio ...
-
bioRxiv - Immunology 2020Quote: ... 14 and 21 of life were homogenized in protein lysis buffer (Tris 50 mM, 2% SDS, 1mM Na3VO4) containing proteinase inhibitors (Complete Mini, Roche Diagnostics) and benzonase (Novagen ...
-
bioRxiv - Immunology 2021Quote: The viral load was inferred on nasopharyngeal swabs through cycle threshold (Ct) determination with Cobas® SARS-CoV-2 Test (Roche), a real-time PCR dual assay targeting ORF-1a/b and E-gene regions on SARS-CoV- 2 genome ...