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Citations for Roche :
801 - 850 of 919 citations for D Fructose U 13C6 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... A single-cell solution was obtained by tissue digestion using 1mgml−1 collagenase D and 0.1mgml−1 DNase I (Roche) in HBSS (Lonza ...
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bioRxiv - Immunology 2021Quote: ... For experiments in which islets were analyzed using flow cytometry single cell suspensions were generated by digestion with 0.4 Wunsch Units/ml Collagenase D (Roche) and 250 µg/ml DNAse I (Roche ...
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bioRxiv - Immunology 2022Quote: ... Collected tissue was mechanically dissociated by chopping followed by enzymatic digestion for 45 minutes at 37°C in DMEM containing collagenase D (1.5 mg/ml, Roche) and DNase I (60 U/ml ...
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bioRxiv - Cell Biology 2021Quote: ... at 37°C in a 0.4 μm filtered solution of 0.5 mg/mL collagenase D (Roche, Indianapolis, IN, USA), 1 Unit/mL Dispase (Stemcell Technologies ...
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bioRxiv - Immunology 2021Quote: ... brain and dura mater were chopped and subject to enzymatic digestion in Hanks’ Balanced Salt Solution (HBSS) containing 2.5 mg/mL collagenase D (Roche) and 0.1 mg/mL DNase I (Roche ...
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bioRxiv - Immunology 2021Quote: ... Livers and liver tumours were excised and then minced with scissors before incubation in RPMI/10% (v/v) FBS/2mM EDTA/1.2mg/mL Collagenase D (Roche)/1mg/mL DNase I (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were dissociated in 1-2 ml of digestion mix (D-PBS, 250ug/ml Liberase TL (Roche; cat: 5401020001), 0.1 mg/ml DNaseI (Sigma ...
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bioRxiv - Immunology 2021Quote: L1/L2 larvae were collected via pipette in 200-300 µl of PBS then added directly to lysing matrix D (1.4-mm ceramic spheres, MagNA Lyser Green Bead tubes, Roche) along with 1 ml of TRIzol LS ...
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bioRxiv - Immunology 2020Quote: ... The organs were chopped mechanically with scalpels and transferred into gentleMACS C tubes containing DMEM supplemented with 1μg/mL Collagenase D (Roche) and 25mg/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... Right lung lobes were chopped finely and digested in pre-warmed RPMI1640 with ‘collagenase cocktail’ (0.625mg ml−1 collagenase D (Roche), 0.425mg ml−1 collagenase V (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2023Quote: ... cells were released by incubating gels in a cell culture incubator in a protease solution of 2.5 mg/mL collagenase D (Roche) in PBS until gels were fully degraded ...
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bioRxiv - Immunology 2022Quote: ... and lung were further digested in RPMI + 50U/mL DNase I + 1mg/mL hyaluronidase + 1mg/mL collagenase D (Roche) on a shaker at 220rpm for 45 minutes at 37°C ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Hippocampus was then sliced with a scalpel and digested for 1 h at room temperature in HBSS supplemented with 0.5 mg/ml collagenase D (Roche) and 10 µg/ml DNase I (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... euthanized mice were perfused with PBS and the left lung lobes digested for 1 h with collagenase D (1.5 mg/ml, Roche) and DNAse A (40 U/ml ...
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bioRxiv - Immunology 2023Quote: ... Popliteal lymph nodes were harvested from immunized mice 1 day later and digested with collagenase D (2.5 mg/mL, Roche) in RPMI-1640 medium supplemented with 10% FBS for 30 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated by dissociating tumor tissue in the presence of collagenase D (1 mg/ml) (Roche-Merck) for 10 min at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Brain and spinal cord were cut into pieces and digested for 30 min at 37 °C with collagenase D (3.75 mg/ml; Roche) and DNase I (1 mg/ml ...
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bioRxiv - Immunology 2023Quote: ... enzymatic digestion of tissue pieces was done with collagenase D (1 mg/ml) and DNAseI (0.1 mg/ml) (both from Roche) in HBSS/10%FBS for 45 min (37°C ...
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bioRxiv - Immunology 2024Quote: ... Lungs were processed with a gentle MACS dissociator (Miltenyi Biotech) according to manufacturer’s protocol and digested with Collagenase D (11mg/ml; Roche) and DNase I (301µg/ml ...
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bioRxiv - Immunology 2024Quote: ... kidneys were cut and digest for 30 min at 37°C with 0.5mg/ml collagenase D (Roche, Mannheim, Germany) and 0.05 mg/ml DNase I (Roche ...
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bioRxiv - Immunology 2024Quote: Lung tissue was prepared by coarsely chopping the tissue followed by incubation in a 0.5 mg/mL solution of collagenase D (Roche) and 500 Units of DNase (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... was used to recover and then amplify the converted DNA fragments through PCR with a maximum of 12 cycles with Kapa HiFi U+ Master Mix (Kapa Biosystems, cat.: KK2801). Barcodes were introduced during this process.
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bioRxiv - Cancer Biology 2020Quote: ... 150 mM NaCl, 1% NP-40, 1 mM EDTA, 5% glycerol, supplemented with 100 U/ml SUPERase-IN and 1X Roche protease inhibitor cocktail) for 10 minutes ...
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bioRxiv - Microbiology 2021Quote: ... The infected PBMCs and CD4+ T cells were cultured at 37ºC in humidified air with 5% CO2 in the presence of 20 U/mL of IL-2 (Roche Diagnosis, Indianapolis, IN, USA), with various concentrations of S100A8 or S100A9 in RPMI-140 (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... and the cDNA template was digested for 30 min at 37 °C after the addition of 1 µl DNase I (10 U/µl; Roche Diagnostics, Rotkreuz, Switzerland), 5 µl 1M Tris/HCl buffer (pH 8.0) ...
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bioRxiv - Microbiology 2022Quote: ... Approximately 800 μg of VLP lysate was then reduced and alkylated as described above and treated with 5 U PNGase F (Roche Cat. Nr. 11365177001) at 37 °C for 12 hours followed by 12 hours with 11 μg of trypsin ...
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bioRxiv - Microbiology 2021Quote: ... tissue pieces were incubated with their epidermal side facing up in RPMI 1640 medium supplemented with 2.4 U/ml Dispase II (Roche Diagnostics GmbH, Mannheim, Germany) overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... 150 mM NaCl, 1% NP-40, 1 mM EDTA, 5% glycerol, supplemented with 100 U/mL SUPERase-IN and 1X Roche protease inhibitor cocktail) for 10 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... one 10 cm plate of Hepa1c1c7 cells at 80% confluence was lysed in 1 mL Pierce IP Lysis Buffer (supplemented with 100 U/mL SUPERase-IN and 1X Roche protease inhibitor cocktail) and incubated on ice for 10 min ...
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bioRxiv - Biochemistry 2021Quote: ... The pellets of harvested bacteria were resuspended in 5 mL of lysis buffer (20mM NaP pH 7.5, 300mM NaCl, 15mM imidazole, 5% glycerol, 0.5mM TCEP, 1 mg/ml lysozyme, 5 U/ml DNase, 1 Roche protease inhibitor tablet/100mL) per 1 g of wet weight culture ...
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bioRxiv - Biochemistry 2021Quote: ... The pellets were resuspended in 5 mL of lysis buffer (20mM NaP pH 7.5, 300mM NaCl, 15mM imidazole, 5% glycerol, 0.5mM TCEP, 1 mg/ml lysozyme, 5 U/ml DNase, 1 Roche protease inhibitor tablet/100mL) per 1 g of wet weight culture ...
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bioRxiv - Immunology 2024Quote: ... pipetting thymus fragments up and down to remove lymphocytes and then digesting the fragments with RPMI 1640 medium containing Liberase (Roche, 0.05 U/ml) plus DNase I (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... and 50 mg of the cells were suspended in 500 μL of ice-cold PBS supplemented with 100 U/mL DNase I (Roche Diagnostics, Mannheim, Germany) to digest extracellular DNA in the colonies ...
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bioRxiv - Immunology 2024Quote: ... THP-1-monocyte-derived IFN-γ-primed macrophages were induced by co-stimulation with PMA and 100 U/ml human IFN-γ (Roche, 11040596001) for 24 h ...
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bioRxiv - Neuroscience 2024Quote: ... 1 μL of each respective primer (5 μM stock) and 0.2 μL (5 U/μL) FastStart Taq DNA Polymerase (Roche Diagnostics GmbH, Mannheim, Germany) in a total volume of 25 μL ...
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bioRxiv - Cell Biology 2020Quote: Cells were pelleted and lysed with 1.5% n-dodecyl-D-maltoside (DDM) in PBS with cOmplete™ protease inhibitor (Roche) for 15 min on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... Dissected and pulverized TA samples were placed in TRIzol in Lysing Matrix D Tubes (MP Biomedical) and were homogenized in a MagNa Lyser machine (Roche) programmed to 7,000 RPM for a total of three 20-second bursts separated by 10-second cool-downs on ice ...
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in complete media (DMEM with 10% FBS and 1% antibiotics) supplemented with Collagenase-D (1 mg/mL; Roche) and incubated at 37°C for 30 min with shaking to form a single-cell suspension ...
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bioRxiv - Microbiology 2020Quote: ... and digested in digest buffer (RPMI supplemented with 10% FBS, 1% penicillin/streptomycin [Invitrogen], 1mg/mL collagenase D [Sigma], 1mg/mL dispase [Sigma], 40 μg/mL DNAse [Roche]) at 37° C for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... The tonsil and lung were gentleMACS dissociated in 5mL digestion buffer (RPMI + 50U/mL DNase I + 1mg/mL hyaluronidase + 1mg/mL collagenase D (Roche)) and then agitated on a shaker at 220rpm for 45 minutes at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting fat pads were rinsed in PBS and minced in ice-cold digestion buffer (7.5 mg/ml Collagenase D (Roche 1108886001), and 4.8 mg/ml Dispase II (Sigma D4693 ...
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bioRxiv - Cancer Biology 2022Quote: ... genomic (g)DNA was extracted both from primary Irf4−/− tumours as well as FACS-sorted control B220+mIgM- BM fr.A-D cells using the High Pure PCR Template Preparation kit from Roche (11796828001). Integrity of resultant gDNA was confirmed in a 2 % Agarose gel ...
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bioRxiv - Immunology 2022Quote: ... were placed in 5mL of digest media (RPMI + 50U/mL DNase I + 1mg/mL hyaluronidase + 1mg/mL collagenase D (Roche)) and homogenized in gentleMACS C tubes (Miltenyi Cat#130096334 ...
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bioRxiv - Immunology 2020Quote: ... mice were perfused with sterile PBS and the right inferior lung lobes were digested at 37°C with 630 µg/ml collagenase D (Roche) and 75 U/ml DNase I (Sigma ...
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bioRxiv - Pathology 2020Quote: Lungs harvested and cut into small pieces were digested (1h, 37c) with 4 mg/ml collagenase D (Roche, Mannheim, Germany). Tissue was meshed through a 40-µm cell strainer ...
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bioRxiv - Immunology 2021Quote: ... the dura mater was carefully removed from the brain and then chopped for enzymatic digestion in Hanks’ Balanced Salt Solution (HBSS) containing 15 mg/mL collagenase D (Roche) and 1 mg/mL DNase (Roche ...
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bioRxiv - Physiology 2020Quote: 1 μg RNA was reverse transcribed into cDNA with Oligo(d)T primers using the Transcriptor First Strand cDNA Synthesis kit (Roche). The qRT-PCR was performed with the LightCycler 480 SYBR Green I Master mix (Roche ...
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bioRxiv - Genomics 2020Quote: ... the tissues were cut into 1-2 mm2 pieces and put in 1mg/ml collagenase and dispase (C/D) solution (Roche Diagnostics GmbH Roche Applied Science ...
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bioRxiv - Immunology 2020Quote: ... Spleens were digested for 30 min at 37°C in digestion buffer [(RPMI 1640 supplemented 0.5 mg/ml collagenase D (Roche; 11088866001) and 10 µg/ml DNase I (Roche ...
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bioRxiv - Immunology 2020Quote: ... were cut into small pieces with a scalpel and incubated in a Collagenase D (1 mg/ml)/ of DNase I (70 μg/ml) enzymatic cocktail (Roche) in RPMI ...