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Citations for Roche :
751 - 800 of 919 citations for D Fructose U 13C6 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The reaction was incubated at 26°C for 20 min before being rapidly quenched by adding stop buffer (1X Recon buffer, 37.5 mM glucose and 0.04 U/μl Hexokinase (Roche Diagnostics, REF:11426362001)) in a 3:10 v/v ratio ...
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bioRxiv - Physiology 2024Quote: ... TA muscles were dissociated and digested in DMEM F/12 medium containing 10 mg/ml of collagenase B and 2.4 U/ml Dispase (Roche Diagnostics GmBH) at 37°C for 30 min and passed through a 30 μm cell strainer ...
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bioRxiv - Cell Biology 2024Quote: ... minced, and digested in 1x PBS containing 150 μg/ml collagenase (MilliporeSigma, C5138) and 20 U/ml DNase I (Roche, 04716728) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Final DNA concentration of 1μM and increasing concentrations of Stl (0.5-8 μM) were mixed with 1μg/ml poly(d[I-C]) (Roche) in EMSA buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Immunology 2021Quote: ... which were subsequently incubated with digestion buffer (IMDM supplemented with 2% FBS, 1 mg/mL Collagenase D [Roche] ...
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bioRxiv - Plant Biology 2021Quote: ... and 0.33 M D-sorbitol supplemented with one tablet (per 50 mL) of cOmplete protease inhibitor cocktail (Roche)] using a Waring blender ...
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bioRxiv - Immunology 2022Quote: ... and then minced and dissociated in digestion buffer (RPMI containing collagenase (1 mg ml−1 collagenase D; Roche), DNase I (100 μg ml−1 ...
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bioRxiv - Microbiology 2022Quote: ... and the right inferior lung lobes were digested at 37°C with 630 µg/mL collagenase D (Roche) and 75 U/mL of DNase I (Sigma–Aldrich ...
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bioRxiv - Immunology 2020Quote: ... The remaining tissue was incubated in digestion buffer (RPMI 1640, 10% FCS, 1.25 mg/ml collagenase D (Roche), 0.85 mg/ml collagenase V (Sigma– Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by a 20-min incubation at 37°C in CSS containing 1.5 mg/ml Collagenase D (Roche), 0.6 mM EDTA ...
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bioRxiv - Immunology 2020Quote: Single cell suspensions of the tumor were obtained after tumor digestion with 400U/mL of collagenase D (Roche) at 37C for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... lung tissue was minced with a scalpel and digested enzymatically with 0.15 WU/mL of D-Liberase (Roche) and 800□U/mL of DNase I (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 10 ml of DMEM media and 1 mg/ml collagenase D (Sigma-Aldrich, COLLD-RO Roche, #11088866001). The tubes were placed on a gentleMACS Dissociator (MACS Miltenyi Biotec ...
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bioRxiv - Genomics 2021Quote: ... The concentration of each 10x single cell and V(D)J library was determined through qPCR (Kapa Biosystems) to produce cluster counts appropriate for the NovaSeq 6000 platform ...
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bioRxiv - Immunology 2021Quote: ... and digested for 20 min at 37 °C in medium supplemented with 1 mg/mL Collagenase D (Roche) and 2000 U/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... Inguinal stromal-vascular fractions (SVF) were isolated from 2-week-old C57BL/6J mice with collagenase D (Roche) digestion and differentiated as described previously34 ...
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bioRxiv - Immunology 2022Quote: ... they were digested for 30 minutes at 37°C in PBS 1X containing 1mg/mL Collagenase D (Roche), 100 U/mL DNAse I (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... Lamina propria-resident immune cells from large intestine were isolated by digesting intestinal tissue with Collagenase D (Roche), Collagenase V (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... Lactate was quantified using a lactate assay kit (D-Lactic/L-Lactic acid UV method, r-biopharm, Roche). All samples were measured using fresh growth medium as control.
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bioRxiv - Bioengineering 2024Quote: ... the infected lungs were transferred to gentleMACSTM C tubes and homogenized on a gentleMACSTM tissue dissociator (“m_lung_01” program) using HEPES buffer (2 mg/ml collagenase D (Roche) and 80 U/mL DNAse I (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: Orthotopically implanted syngeneic KPC3 tumor tissues were harvested and digested with 1 mg/mL Collagenase D (Roche, Switzerland) plus with 100 μg/mL DNase I (Roche ...
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bioRxiv - Immunology 2023Quote: ... Lungs were perfused with sterile PBS and digested for 1 hour with 625µg/mL collagenase D (Roche 11088875103) and 75U/mL DNase I (Sigma D4527) ...
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bioRxiv - Immunology 2023Quote: ... the lung was perfused with PBS and digested for 45 minutes with 625μg/mL collagenase D (Roche, 11088875103) and 75U/mL DNase I (Sigma ...
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bioRxiv - Neuroscience 2023Quote: Histological staining included terminal transferase mediated d-UTP nick end labelling (TUNEL) with Co/Ni enhancement (Roche, UK) performed following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Cells were isolated from the brain by mechanical disruption followed by collagenase D digestion (Roche Pharmaceuticals, Mannheim, Germany). The resulting cell suspension was resuspended in 40% Percoll (GE Healthcare Biosciences ...
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bioRxiv - Immunology 2023Quote: Lungs were perfused with sterile PBS and digested for 45 minutes with 625μg/mL collagenase D (Roche 11088875103) and 75U/mL DNase I (Sigma D4527) ...
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bioRxiv - Physiology 2024Quote: ... samples were diluted using buffer D (20mM HEPES, pH7.9, 10% Glycerol, 1mM EDTA, 1mM DTT, 0.5mM PMSF, 10mM NaF, Roche PhosSTOP ...
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bioRxiv - Microbiology 2024Quote: ... and its activity was measured by addition of 1 mM chlorophenol red-β-D-galactopyranoside (Roche Diagnostics, GmbH) in 100 mM HEPES pH 8 (Dutscher) ...
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bioRxiv - Neuroscience 2024Quote: ... then resuspended in 2mL of BSA-free HBSS supplemented with 2.5 mg/mL Collagenase D (Cat. #11088858001; Roche) and 12.5 μL of 0.5 mg/mL DNAseI (Cat ...
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bioRxiv - Immunology 2024Quote: ... and then minced and dissociated in digestion buffer (RPMI containing collagenase (1 mg ml−1 collagenase D; Roche), DNase I (100 μg ml−1 ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue pieces were then moved to a 6-well plate and incubated with Collagenase D (0.24U/mg, Roche) and DNase I (10U/µl ...
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bioRxiv - Immunology 2024Quote: ... Digestion was performed for 30 min at 37⍰C with 2 mg/ml collagenase D (Roche, Meylan, France), 1 mg/ml dispase (Invitrogen ...
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bioRxiv - Immunology 2024Quote: Spleens and thymi were injected with an enzyme mixture containing 0.2% collagenase D (Roche Diagnostics, Indianapolis, IN, USA) and 0.01% DNase I (Roche ...
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bioRxiv - Immunology 2024Quote: ... Tissue pieces were further finely minced (∼0.5 cm) and incubated in RPMI 1640 with 0.5 mg/ml collagenase D (Roche) and 2% FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA was diluted 3-fold with nuclease-free water and 1.5 µL was PCR-amplified using 0.15 U KAPA HiFi HotStart DNA Polymerase (Kapa Biosystems, Wilmington, MA, USA) in a final volume of 10 µL ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were dehydrated and rehydrated in ethanol and water and incubated with 10 μM dNTP mix containing Digoxigenin-dUTP and 20 U/mL Klenow Fragment (Roche; Basel, Switzerland) at room temperature for 2 h ...
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bioRxiv - Cancer Biology 2020Quote: ... 40 mM Tris-HCl pH 7.5, 20 mM MgCl2, and 4% Triton X-100 supplemented with 100 U/ml SUPERase-IN and 1X Roche protease inhibitor cocktail), and 24 ml nuclease free water on ice for 20 min with frequent mixing ...
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bioRxiv - Cancer Biology 2020Quote: ... An overnight blunt ligation was induced for the 5’ of exon 1 and the 3’ of exon 3 (5 U T4 DNA ligase (Roche, Basel, Switzerland)) at room temperature ...
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bioRxiv - Pathology 2023Quote: Human normal and IPF lungs were minced with a razor blade in a 100 mm petri dish in a cold DMEM medium containing 0.2 mg/ml Liberase DL and 100 U/ml DNase I (Roche, Indianapolis, IN, USA). The mixture was transferred into 15 ml tubes and incubated at 37 °C for 35 min in a water bath under continuous rotation to allow enzymatic digestion ...
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bioRxiv - Pathology 2023Quote: ... The lungs were immediately harvested and minced with a razor blade in a 100 mm petri dish in a cold DMEM medium containing 0.2 mg/ml Liberase DL and 100 U/ml DNase I (Roche, Indianapolis, IN, USA). The mixture was transferred into 15 ml tubes and incubated at 37 °C for 35 min in a water bath under continuous rotation to allow enzymatic digestion ...
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bioRxiv - Cell Biology 2023Quote: ... Livers were collected on ice and incubated in digestion buffer (0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum) at room temperature ...
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bioRxiv - Cell Biology 2024Quote: Human peripheral blood mononuclear cells isolated from buffy coats were activated in vitro for 1 week with 20 U/ml of IL-2 (Teceleukin; Roche, Nutley, NJ) and 5 μg/ml phytohemagglutinin (Roche ...
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bioRxiv - Cancer Biology 2020Quote: ... and pancreatic tumor bearing mice by mechanical separation of the tumor followed by digestion with an enzymatic cocktail consisting of 1mg/ml collagenase A and collagenase D and 0.4mg/ml DNase I (all from Roche) in PBS at 37°C for 2-3h with rotation at 600rpm ...
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bioRxiv - Immunology 2021Quote: ... Lungs were dissected and digested for 30min at 37°C using RPMI media (2% FBS+ Pen/Strep, glutamine, 10mM HEPES) containing 0.5mg/mL Collagenase D (Roche). The digested fragments were passed through a 70μm cell strainer (Greiner bio-one ...
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bioRxiv - Biophysics 2021Quote: ... 0.1% (v/v) 2-mercaptoethanol and 60 × 10−3 М n-Octyl-β-D-Glucopyranoside) containing protease inhibitor (Roche). Total protein was collected and incubated with anti-p75NTR (Alomone ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg poly[d(I-C)] and 0.1 μg poly-Lysine (based on DIG Gel Shift Kit, 2nd Generation, Roche) for 15 min at RT ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The imaginal discs were removed and suspended whole in 350μl of sucrose solution (250mM D-sucrose, 10mM Tris-HCl, 1mM. MgCl2, 1x protease inhibitors (Roche)) inside labelled 2ml dounce homogenisers (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... C-reactive protein and D-dimer levels were measured using Cobas Integra 400 plus analyzer (Roche Diagnostics Nederland B.V.).
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bioRxiv - Immunology 2021Quote: ... ankle joints were teased apart and shaken at 110 rpm at 37°C for 25 minutes with 2.68mg/mL collagenase D (Roche) in RPMI 1640 (Gibco ...
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bioRxiv - Cell Biology 2022Quote: Tissue leukocytes were extracted from surgically dissociated lung tissue by enzymatic digestion with 2ml enzyme mix (RPMI with 0.625 mg/ml collagenase D (Roche), 0.85 mg/ml collagenase V (Sigma-Aldrich) ...