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Citations for Roche :
801 - 850 of 1954 citations for Centromere Protein O CENPO Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with 30 μl of pre-washed Protein A agarose bead slurry (#11134515001, Roche) for an additional hour (4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... and 250 mg or 500mg protein was digested with 20 µg/ml proteinase K (PK) (Roche) for PrPSc blots for 1 hour at 37C ...
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bioRxiv - Microbiology 2020Quote: ... Protein lysates were obtained by lysing cells in 0.1% SDS and protease inhibitor cocktail tablets (Roche #04693124001). Cell lysates were separated by SDS-PAGE (Bio-Rad 4-20% Mini-PROTEAN TGX Precast Gel ...
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bioRxiv - Molecular Biology 2019Quote: ... proteins were digested by addition of 40 µl proteinase K (Roche #03115852001, dissolved to 10 µg/µl) and incubation for 2 hours at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... proteins were digested by addition of 40 µl proteinase K (Roche #03115852001, dissolved to 10 µg/µl) and incubation for 2 hours at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 µl anti-CENP-ACnp1 (sheep in-house antiserum) and 25 µl Protein-G-Agarose beads (Roche) or 2 µl of anti-GFP (A11122 ...
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bioRxiv - Cell Biology 2019Quote: ... samples were further incubated for 2 hrs in 40 µl pre-cleared protein-G agarose beads (Roche). Beads with immunocomplexes were centrifuged at 3,000 × g and washed four times in lysis buffer with intermittent incubations ...
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bioRxiv - Genetics 2019Quote: Total protein was extracted from transfected HEK293 cells lysed in RIPA buffer (Pierce) containing protease inhibitors (Roche) 24 hours post transfection and size separated using SDS-PAGE ...
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bioRxiv - Biochemistry 2019Quote: ... and then scraped in 10 ml PBS + 1X Protein Inhibitor Cocktail buffer (catalog no. COUL-RO, Roche) and centrifuged for 5 min at 1,000 x g at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... The eluted proteins were subjected to 10% SDS-PAGE gels for immunoblot analyses using anti-GFP (Roche) and anti-Myc (Roche ...
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bioRxiv - Microbiology 2021Quote: ... transferred to PVDF membranes and protein expression was detected using anti HA (3F10, Roche 11867423001, 1:5000) followed by horseradish peroxidase (HRP)-conjugated anti-rat antibodies (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse/rabbit IgG overnight at 4°C and further with protein G-coupled agarose beads (ROCHE) for 1-2 h ...
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bioRxiv - Cell Biology 2020Quote: Cells were lysed and proteins were purified by Complete™ Lysis-M EDTA-free kit (Roche, 04719964001). Protein samples were run on Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: Total proteins were extracted from cells using RIPA lysis buffer with a protease inhibitor cocktail (Roche, Germany). The proteins were quantified using Thermo Scientific Pierce BCA Protein Assay ...
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bioRxiv - Microbiology 2020Quote: Protein lysates of HEK-293T cells were extracted with RIPA buffer (PIERCE) and protease inhibitor cocktail (Roche). Loading buffer (Life Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: Cells were harvested, lysed in RIPA buffer (Cell Signalling, 9806) containing a protein inhibitor cocktail (Roche, 11873580001), and centrifuged at 4°C for 10 min at 12,000 g/min ...
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bioRxiv - Molecular Biology 2020Quote: ... (a) Proteins were extracted from cryogrinded cell powders with different extraction solutions supplemented with protease inhibitors (Roche) (solution compositions listed in Supplementary Table 1) ...
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bioRxiv - Cell Biology 2019Quote: ... Cells expressing GST fusion proteins were resuspended in PBS containing protease inhibitor cocktail (Roche Diagnostics, Penzberg, Germany) and lysed by lysozyme treatment (1 mg/ml ...
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bioRxiv - Bioengineering 2020Quote: ... and protein was extracted via Dounce homogenizer in a cocktail of RIPA buffer (Thermo) and proteinase (Roche) supplemented with phosphatase (Roche ...
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bioRxiv - Systems Biology 2019Quote: ... Proteins from fracture femora were extracted by RIPA buffer combined with protease inhibitors and phosphatase inhibitors (Roche). The supernatants were obtained by centrifugation (12,000g ...
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bioRxiv - Cell Biology 2020Quote: Whole cell or mitochondrial samples were lysed in RIPA buffer supplemented with cOmplete protein inhibitor cocktail (Roche) and 1mM phenylmethylsulfonyl fluorid (PMSF) ...
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bioRxiv - Genetics 2020Quote: ... we assessed levels of protein in mice urine using Chemstrip® 10 (Roche Diagnostics GmgH, Mannheim, Germany).
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bioRxiv - Microbiology 2020Quote: Protein was extracted from cells using Radioimmunoprecipitation assay (RIPA) lysis buffer containing 1X cOmplete Protease InhibitorCocktail (Roche) and 1X Phenylmethylsulfonyl fluoride (Sigma Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... and 500-750 μg lysate was pre-cleared with Protein G agarose beads (Roche; Cat. No.11719416001) for 1 hour at 4°C with rotation at 5 rpm in a rotator ...
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bioRxiv - Plant Biology 2022Quote: ... For every biological replicate 500 μg protein was digested with sequencing grade trypsin (1:100 trypsin:protein; Roche) overnight at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... HA:SQSopsin was immunoprecipitated from the detergent-soluble fraction using 70 μl of 50% protein G resin (Roche) slurry and anti-HA antibody (12CA5) ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were pre-cleared by incubating with 30 μl of protein G-agarose beads (Roche, cat. # 11719416001) for 3 h at 4°C on mini-rotator ...
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bioRxiv - Cell Biology 2023Quote: Testis protein extraction was performed in RIPA buffer with a cocktail of protease inhibitors (Roche cat. 11836170001), which was freshly added before beginning protein extraction ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... Total protein was extracted with radio immune precipitation assay (RIPA) buffer containing phenylmethylsulphonyl fluoride (PMSF) (Roche, Switzerland) and a protease inhibitor cocktail (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then either immunoprecipitated by adding 2 μg mouse anti-GFP (Clones 7.1 and 13.1, Roche) and protein G sepharose beads or ALFA selector ST beads (NanoTag Biotechnologies) ...
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bioRxiv - Neuroscience 2023Quote: ... pH 7.5) was prepared with one tablet of protein inhibitor cocktail (cOMPLETE Mini lacking EDTA, 11873580001, Roche) along with 15uL of RNAsin (N2615 ...
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bioRxiv - Physiology 2023Quote: ... cells/tissue were lysed in mammalian protein extraction reagent (M-PER) supplemented with protease inhibitor cocktail (Roche). Soluble proteins and insoluble matrix were separated by centrifugation at 10,000 × g ...
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bioRxiv - Plant Biology 2024Quote: ... The expression of the 35S-driven IAR3 gene was monitored by protein immunodetection with anti-His (Roche) and anti-mouse IgG-peroxidase (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: Proteins were extracted using 1xPBS pH 7.4 with Protease Inhibitor Cocktail (1 tablet/10 mL) (Roche Diagnostics) and sonicated during 10 seconds at 10% power ...
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bioRxiv - Cell Biology 2020Quote: ... Immuno blotting was performed using anti-HA antibody 3F10 (Roche, IN) at 1:2000 dilution ...
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Resurgent Na+ currents promote ultrafast spiking in projection neurons that drive fine motor controlbioRxiv - Neuroscience 2021Quote: ... incubated with alkaline phosphatase conjugated anti-DIG antibody (Roche, 1:600), and developed overnight in BCIP/NBT chromagen (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary antibody were rat anti-HA (MAb 3F10 1:20, Roche) or rabbit anti-FLAG (M2 ...
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bioRxiv - Cell Biology 2022Quote: Cells were stained with antibodies against GFP (Roche; 1181446001; 1:1000), GFP (Abcam ...
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bioRxiv - Developmental Biology 2019Quote: ... overnight incubation in anti-DIG antibody solution (1/2000; Roche #11093274910) at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was incubated with mouse polyclonal anti-GFP antibody (Roche) (1:1000) ...
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bioRxiv - Developmental Biology 2019Quote: ... by using peroxidase-conjugated primary antibodies against fluorescein (1:250, Roche) and digoxigenin (1:50 ...
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bioRxiv - Developmental Biology 2019Quote: ... The following antibodies were used: sheep anti-Digoxigenin (1:2000; Roche), sheep anti-Digoxigenin conjugated with alkaline phosphatase (1:2000 ...
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bioRxiv - Cell Biology 2021Quote: ... the supernatants were incubated with anti- GFP antibody (Roche, cat.no. 11814460001) coupled to protein G Dynabeads (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... Primary antibodies used were rat anti-HA (1:200, Roche, 3F10), rabbit anti-NeuN (1:200 ...
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bioRxiv - Cell Biology 2021Quote: Antibodies used for western blots were α-HA clone 12CA5 (Roche), α-myc 9E10 (Babco) ...
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bioRxiv - Plant Biology 2020Quote: ... Coimmunolocalization was performed with a rabbit anti GFP primary antibody (Roche) and appropriate combination of Alexa-conjugated secondary antibodies (Invitrogen).
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bioRxiv - Plant Biology 2021Quote: ... which was then blotted with α-HA antibodies (Roche, Cat # 12013819001).
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies used were mouse anti-HA 12CA5 (Roche; 1:1000), mouse anti-HA 11 (Biolegend, ...
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bioRxiv - Molecular Biology 2021Quote: Monoclonal anti-HA antibody (3F10, 1:1000) was purchased from Roche. Monoclonal anti-GFP (B-2 ...