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Citations for Roche :
8351 - 8400 of 8559 citations for 6 Methyl 2 3 4 9 tetrahydro 1H carbazol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... ChIPs were performed by combining sonicated chromatin (500 ng DNA content) with either 1 μg mouse mAb α-GFP (Roche Diagnostics #11814460001) or 1 μg rabbit α-PfHP1 12 in incubation buffer [(5% Triton-X-100 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl of TruSeq PCR primer cocktail (50 μM) and 20 μl of KAPA HiFi HotStart ReadyMix (KAPA Biosystems, cat. no. KK2601). USER digestion was performed at 37 °C for 15 min ...
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bioRxiv - Plant Biology 2021Quote: Frozen inflorescence tissue was ground with liquid nitrogen and resuspended in lysis buffer (50 mM Tris pH 8, 150 mM NaCl, 5 mM MgCl2, 10% glycerol, 1% IGEPAL, 0.5 mM DTT, 1mM PMSF, 1X Roche protease inhibitor cocktail) and homogenized with mixing for 15 minutes at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... containing Roche mini-complete protease inhibitor EDTA-free and Roche PhosSTOP (1 tablet of each per 10 mL of buffer) (Roche, Basel, Switzerland). Samples (on ice ...
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bioRxiv - Biochemistry 2021Quote: ... for 10 min and then harvested using 1 ml of ice-cold 1X PBS supplemented with 0.01% Phosphatase inhibitor (PhosSTOP, Roche, Cat. no. 04906845001). Pellets corresponding to 10 plates of a 10 cm plate were pooled together and centrifuged at 5,000 rpm for 5 min at 4°C ...
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bioRxiv - Genetics 2021Quote: ... Hybridizations of the probe with the RNA were detected with an alkaline phosphatase-conjugated antibody (Anti-Digoxigenin-AP, Fab Fragments, 1:5000, Roche, Cat# 11093274910). Finally ...
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bioRxiv - Microbiology 2021Quote: Bacteria-captured beads were incubated at 37°C for 1 hour with 10 mg/mL of lysozyme (Cat # 10837059001, Roche, Basel, Switzerland) diluted in 10 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... Lung lobes were then placed in 1 milliliter Triazol and homogenized with zirconia beads with a MagNA Lyser instrument (Roche Life Science).
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bioRxiv - Bioengineering 2019Quote: ... Fluorescence scanning was performed from 25°C- 95°C at a rate of 1°C/min using a Lightcycler 480 Instrument (Roche Life Scientific). Melting temperatures were calculated from the inflection point in the first-derivative curve.
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bioRxiv - Evolutionary Biology 2022Quote: ... we mixed 5 μl of crude lysate with 45 μl of TNG buffer (100 mM Tris, 100 mM NaCl, 10% glycerol, 10 mM DTT, 1×cOmplete™ (EDTA-free Protease Inhibitor Cocktail, Roche 11873580001), pH 7.2-7.5) ...
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bioRxiv - Cell Biology 2022Quote: Isolated mouse islets and MIN6 cells were lysed in Tris-lysis buffer (10 mM Tris-Cl pH 7.5, 100 mM NaCl, 1% Triton X-100, 10% glycerol, and cOmplete™ protease inhibitor cocktail [Roche, Basel, Switzerland]) on ice ...
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bioRxiv - Epidemiology 2020Quote: ... plasmids or DNA samples (Table 1) by real-time TaqMan PCRs assays on a LightCycler® 480 (LC480) (Roche Applied Science, Germany). Real-time PCR assays were performed with LightCycler® 480 Probe Master Mix 1× (Roche Applied Science ...
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bioRxiv - Immunology 2019Quote: ... assay (dynamic range 20-10m) prior to 2010 and using the COBAS Ampliprep/COBAS TaqMan HIV-1 Test version 2.0 by Roche (CAP/CTM v2.0) (dynamic range 20-10m subsequent to 2010 ...
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bioRxiv - Developmental Biology 2019Quote: ... These steps were followed by an incubation of the embryos in anti-digoxigenin-POD (peroxidase) Fab fragments (Roche, 1:50 in TNB). The riboprobe signals were finally detected using fluorescein-tyramide (FITC ...
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bioRxiv - Developmental Biology 2019Quote: ... The samples were boiled at 95°C for 5 minutes and diluted 1:1000 in dilution buffer provided in ATP luminescence kit HSII (Roche, Cat# 11699709001). The further assay was performed as instructed by the kit’s manual in Luminometer (Promega GloMax96 Microplate Luminometer) ...
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bioRxiv - Microbiology 2019Quote: HA-tagged constructs for western blotting were detected using a 1:5000 dilution of anti-HA-peroxidase antibody (Roche clone 3F10, #12013819001). HA-tagged constructs for indirect immunofluorescence were detected using anti-HA (Zymed ...
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bioRxiv - Physiology 2019Quote: Quantitative real time PCR (qPCR) was performed with primer sets (Supplemental Table 1) designed using Roche Universal Probe Library Assay Design Center (Roche Diagnostics, UK) in conjunction with corresponding FAM-labelled probes ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were washed twice in 500 µl SPC buffer (1 M Sorbitol, 20 mM PIPES pH 6.3, 0.1 mM CaCl2, with Roche cOmplete protease inhibitors (Sigma)) and spun at 2k RPM for 3 minutes at 4°C between washes ...
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bioRxiv - Plant Biology 2020Quote: ... In situ nick-end labeling of nuclear DNA fragmentation was performed for 1 h in the dark at 37°C using the In-Situ cell death detection kit (Roche Applied Science) according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 mio cells were lysed in 100 μl Buffer-B (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1% SDS, 1x Roche cOmplete protease inhibitors) and sonicated in a microtube (Covaris ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl of immunoprecipitated RNA was used for quantification of mRNA expression levels by RT-qPCR as described in the section ‘Quantitative PCR’ except that DNase treatment was omitted and 1 μg yeast RNA (Roche, Basel, Switzerland) was added in the cDNA reaction.
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bioRxiv - Cell Biology 2020Quote: ... Mip1α and housekeeping gene β-actin was evaluated by quantitative RT-PCR using the LightCycler 480 SYBR Green 1 Master kit and LightCycler 480 II (Roche, Indianapolis, IN) and oligos ...
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bioRxiv - Microbiology 2020Quote: ... Treatment of organs was as followed: PBS supplemented with protease inhibitor (1 tablet cOmplete protease inhibitor, Roche in 35 mL of PBS) was added to the frozen organs (volume depending on the weight of the organ ...
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bioRxiv - Pathology 2020Quote: ... Microwave decloaking was performed in 10 mM sodium citrate (pH 6.0) and non-specific sites were blocked in TBSTw containing 1% Blocking Reagent (Roche Diagnostics, Indianapolis, IN), 5% normal goat or donkey sera ...
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bioRxiv - Neuroscience 2019Quote: ... The PVDF membranes were treated and immersed into the rabbit anti-mouse PSD-95 monoclonal antibody (1:1000, Roche Group, Nutley, USA) to incubate at 4 °C overnight ...
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bioRxiv - Biochemistry 2019Quote: Cultured cells were lysed in HEPES-buffered saline (HBS) with 1% NP-40 supplemented with protease inhibitor cocktail (complete protease inhibitor cocktail tablets; Roche Applied Science) on ice for 20 mins ...
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bioRxiv - Plant Biology 2019Quote: ... and 1 µg RNA was used for cDNA synthesis with oligo(dT)12-18 using Transcriptor first-strand synthesis kit (Roche, Basel, Switzerland). Transcript levels were measured by qRT-PCR using LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Genomics 2021Quote: ... For a 15 ul reaction 1 ul of undiluted cDNA was combined with 1x FastStart Universal Probe Master Mix with Rox (Roche Diagnostics, Australia), 50 nm of the forward and universal reverse primer ...
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bioRxiv - Cell Biology 2021Quote: ... 1% Triton X-100, and 1 mM EDTA) containing a phosphatase inhibitor cocktail (Nacalai Tesque, Kyoto, Japan) and protease inhibitor cocktail (Roche, Basel, Switzerland). After centrifugation at 20,380×g for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... 100 mM KCl, 5 mM MgCl2, 1 mM dithiothreitol, 5% glycerol, and 0.1% Triton X-100 supplemented with Roche Protease Inhibitor cocktail) and then roc ked for 10 min at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... TES (Kieser et al. 2000) was used for transformation and all fusion experiments supplemented with 1 mg/mL DnaseI (Roche Diangnostics GmbH). Antibiotics apramycin (Duchefa Biochemie ...
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bioRxiv - Biophysics 2020Quote: ... Pellets were resuspended in lysis buffer (1 M NaCl, 20 mM Tris, 20 mM imidazole, Roche EDTA-free protease inhibitor, pH 7.5) and lysed by sonication ...
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bioRxiv - Immunology 2020Quote: ... The cDNA samples were diluted 1:10 in water and analyzed in triplicate using a LightCycler 480 detection system (Roche, Meylan, France). PCR conditions were 95°C for 5 min and 45 cycles of 95°C for 15 s ...
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bioRxiv - Cell Biology 2021Quote: ... and finally washed with HK buffer (25mM HEPES pH 7.2, 150mM KCl, 1mM DTT, 1 mM EDTA, Protease Inhibitors (Roche, Complete EDTA free)) ...
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Glucocorticoid receptor collaborates with pioneer factors and AP-1 to execute genome-wide regulationbioRxiv - Genomics 2021Quote: ... followed by addition of 100 μL ChIP Elution Buffer (50 mM Tris-HCl, pH 7.5 @ RT, 10 mM EDTA, 1% SDS) and 20 μL Pronase (Roche, 20 mg/mL) for incubation at 42°C for 2 hours ...
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bioRxiv - Cell Biology 2022Quote: ... and processed in digestion buffer (1 mg/ml of Collagenase D and 80 U/ml DNase I, both from Roche, in DMEM) and a GentleMACS dissociator (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2022Quote: ... The mRNA abundance for the genes (Supplementary Table 1) of interest was determined by quantitative PCR using Fast Start SYBR Green (Roche, Cat# 4673484001). The data was quantified by the comparative ΔCT method and expressed relative to β-actin mRNA.
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bioRxiv - Genomics 2022Quote: ... nuclei were incubated with streptavidin conjugated secondary antibody diluted 1:100 in Wash Buffer (20 mM HEPES pH7.5, 150 mM NaCl, 0.5 mM spermidine, Roche Complete Protease Inhibitor Cocktail) for 45 minutes at RT ...
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bioRxiv - Microbiology 2022Quote: ... Adapter ligation was performed for 1 hour at 20°C using the Kapa Unique-Dual Indexed Adapters diluted to 1.5 μM concentration (Roche Sequencing Solutions Inc.). After ligation ...
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bioRxiv - Molecular Biology 2022Quote: ... ESCs or HEK293T cells were harvested and lysed in TEN buffer (50 mM Tris-HCl, 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 0.5% Na-Deoxycholate, supplement with Roche cOmplete Protease Inhibitor). The lysates were quantified by the Bradford method and equal amount of proteins were loaded for Western blot assay ...
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bioRxiv - Pathology 2022Quote: RNA was reverse-transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (LifeTechnologies, Carlsbad, CA) on the LightCycler 480 or LC96 instrument (Roche, Indianapolis, IN), and quantified by interpolation onto a standard curve made up of serial tenfold dilutions of in vitro transcribed RNA ...
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bioRxiv - Molecular Biology 2023Quote: ... and pIZT-CS2 (1 μg) containing the complete cDNA sequence of BmCPV S2 dsRNA encoding RdRp using Roche-X Gem (Switzerland, Roche, Cat: 6366236001). At 48 h posttransfection ...
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bioRxiv - Cancer Biology 2024Quote: ... Exome libraries were prepared from 1 μg of genomic DNA from each analyzed section using the Nimblegen EZ Exome kit V3 (Roche, Nutley, NJ). Paired-end 100 bp sequencing was performed on a HiSeq2500 sequencer (Illumina Inc. ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA was isolated by isopropanol precipitation of cells and tumor lysates (lysis buffer was Tris [pH 8] 100 mM, EDTA 5 mM, SDS 0.2%, NaCl 200 mM, and 1 mg/mL proteinase K [Roche Diagnostics, Mannheim, Germany]) and resuspended in Tris/EDTA (TE ...
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bioRxiv - Cell Biology 2024Quote: ... or 48 h after seeding in a Triton X-100-based buffer (150 mM NaCl, 20 mM Tris-HCl pH 7.5, 5 mM EDTA, 1% Triton X-100, Roche protease inhibitor cocktail). A linear sucrose density gradient was prepared in 13x51 mm centrifuge tubes (Beckman Coulter ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with a solution containing horseradish peroxidase (HRP)- conjugated anti-Dig (at a dilution of 1:500; #11207733910, Roche Applied Science) and goat anti-mCherry (at a dilution of 1:250 ...
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bioRxiv - Immunology 2024Quote: ... Tumours were dilacerated using scalpels to obtain <1mm sized pieces and resuspended in R10 supplemented with 1 mg/mL Liberase TL (Roche, Cat. 5401020001) and 10 μg/mL DNase I (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... The qPCR was performed using PerfectStartTM Green qPCR Super Mix (TransGen Biotech, 1) on a Real-time PCR Detection System (Roche, LightCycle480 II). RPLP0 served as an internal control ...
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bioRxiv - Microbiology 2023Quote: ... Vero/TMPRSS2 cells (JCRB Cell Bank, Cat# JCRB1818) were cultured in DMEM containing 5% FBS and 1 mg/mL G418 (Roche, Basel, Switzerland). VeroE6/TMPRSS2 cells (JCRB Cell Bank ...
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bioRxiv - Microbiology 2023Quote: ... 50 mM Tris-HCl, 10% glycerol, 1% IGEPAL [Sigma, St Louis, MO], and cOmplete EDTA-free protease inhibitor cocktail [Roche] [pH 7.4]). Cells were vortexed ...