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Citations for Roche :
8201 - 8250 of 8559 citations for 6 Methyl 2 3 4 9 tetrahydro 1H carbazol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... For quantitative-RT PCR analysis 1 µg of total RNA of each sample was reversed-transcribed to cDNA (Transcriptor First Strand cDNA Synthesis kit, Roche 04379012001), cDNA was diluted 1:20 and used as template for amplification reactions ...
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bioRxiv - Zoology 2019Quote: cDNA (1 μL) from control larvae was amplified using the KAPA HiFi HotStart DNA Polymerase Taq (Kapa Biosystems, Wilmington, MA, USA), insect-HSP70 degenerate primers (from Bernabò et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... the lysed cells were resuspended in LB2 (10 mM Tris-HCl, pH 8.0, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, Roche cOmplete protease inhibitors) and incubated at 35 rpm for 5 min followed by centrifugation ...
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bioRxiv - Genomics 2019Quote: ... transcripts derived from self-reporting transposons) involved mixing 1 µl cDNA template with 12.5 µl Kapa HiFi HotStart ReadyMix (Kapa Biosystems #KK2601), 0.5 µl 25 µM SMART primer ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... sample pellets were resuspended in 200 µL lysis buffer (100 mM triethylammonium bicarbonate buffer (TEAB) with 1% (v/v) sodium deoxycholate (SDC) and 1x protease inhibitor cocktail solution (Roche, Switzerland)) ...
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bioRxiv - Immunology 2020Quote: ... The resulting membrane pellet was resuspended in ice cold 500 μl TNE buffer (10 mM Tris/Cl [pH 7.4], 150 mM NaCl, 5 mM EDTA, 1% Triton X-100 [Sigma], 10X protease inhibitors [Complete tablets, Roche, Indianapolis, IN]). Sucrose gradients for the preparation of lipid rafts were assembled previously described (18) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Endogenous peroxidase activity was blocked by incubation with 3% H2O2 for ten minutes followed by two washes with PBS containing 0.1% Tween-20 and blocking with PBS containing 1% bovine serum albumin (Roche, Mannheim, Germany) and 0.2% skim milk powder (Easis ...
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bioRxiv - Genomics 2020Quote: ... cells were washed with buffer MB#1 (10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.2% NP-40, 1x Roche complete EDTA-free). Chromatin was fragmented with MNase for 10 min at 37°C and digestion was stopped with 5 mM EGTA at 65°C for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... and subjected to a protease protection assay by resuspension in 250 μl of freshly prepared 1 mg/mL Pronase (Roche, 10165921001)/0.01% Saponin/PBS at 12°C for 1h ...
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bioRxiv - Molecular Biology 2020Quote: ... rocked at 4°C for 10 min and after centrifugation resuspended in 10 ml ice cold buffer LB2 (10 mM Tris-HCl pH 8.0, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1x Roche cOmplete protease inhibitors), rocked at 4°C for 5 min and centrifuged again ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μl of each unpurified amplicon was then carried to a second PCR reaction using KAPA HiFi Library Amp (Roche Sequencing). NEBNext Multiplex Oligos (New England Biolabs ...
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bioRxiv - Physiology 2021Quote: ... Collagen was then extracted from the tissue samples using a salt extraction buffer (1 M NaCl, 25 mM EDTA, 50 mM Tris-HCl, pH 7.4) containing protease inhibitors (Roche, Basel, Switzerland). Samples were extracted overnight at 4°C with agitation ...
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bioRxiv - Neuroscience 2020Quote: ... After centrifugation, cells were lysed (750 mM NaCl, 10 mM Tris, 1 mM EDTA pH 7.6, protease inhibitor (Roche, Mannheim, Germany)) ...
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bioRxiv - Genomics 2021Quote: ... Four PCR reactions were then carried out with 1 μl of the purified DNA using the KAPA HotStart HiFi Ready Mix (KAPA Biosystems) for 10 cycles inserting a 15 nt homology sequence for the subsequent cloning step (IF_fwd/IF_rev) ...
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bioRxiv - Immunology 2021Quote: ... and were enzymatically digested in a shaker incubator at 37°C for 40 minutes in RPMI medium containing 1 mg/ml Collagenase D (Roche-Diagnostics), 1 mg/ml hyaluronidase and 50 U/ml DNase I (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed in Tris-buffered saline (TBS) supplemented with 1% Triton X-100 and protease/phosphatase inhibitor cocktails (Roche Diagnostics), the insoluble material was cleared by centrifugation for 10 min at 20,000 × g ...
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bioRxiv - Evolutionary Biology 2021Quote: ... For large-scale purification cells were resuspended in lysis buffer (50 mM NaPO4 pH=7.0; 250 mM NaCl; 1 tablet complete protease inhibitor EDTA free (Roche, Basel, Switzerland) per 50 mL ...
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bioRxiv - Physiology 2021Quote: ... containing cOmplete EDTA-free protease inhibitor cocktail at a concentration of 1 tablet/7 ml (Roche Applied Science, Indianapolis, IN, USA)) by repeated passage through a 20-gauge needle to obtain plasma membrane-enriched preparations ...
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bioRxiv - Physiology 2020Quote: ... was homogenized by probe sonication in ice-cold membrane buffer (50 mM Tris-HCl pH 7.4, 1 mM EDTA, 250 mM sucrose, and complete protease inhibitors table (Roche Diagnostics, A32965)) ...
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bioRxiv - Microbiology 2021Quote: ... the beads were collected on a magnetic stand for 2 min and washed four times with lysis buffer and four times with wash buffer (50mM Tris pH 7.6, 300mM NaCl, 1% Triton X-100, + complete protease inhibitor/ Roche Cat# 11836153001). The beads were resuspended in 1x Laemmli sample buffer ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated in 1% Triton X-100 for 30 min on ice followed by treatment with proteinase K (Roche 3115879001) at the indicated concentrations at 37°C for 5 min ...
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bioRxiv - Cell Biology 2020Quote: ... Fusion was carried out by resuspending the mixed cell pellet slowly in 1 mL of 50% PEG 1500 (Roche, Basel, Switzerland) at 37°C over the course of 1 minute and incubated for a further 1 minute at 37°C ...
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bioRxiv - Cell Biology 2020Quote: Cells were lysed in lysis buffer (50 mM Tris-HCl, pH 7.8, 150 mM NaCl, 1% Nonidet-P40 and protease inhibitor cocktail tablets (Roche, Basel, Switzerland)) ...
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bioRxiv - Microbiology 2020Quote: Frozen fecal samples were diluted in lysis buffer [100mg feces per 1 ml of PBS containing protease inhibitors (Roche, Indianapolis, IN)] ...
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bioRxiv - Molecular Biology 2021Quote: ... Planarians were treated with 5% NAC for 5 minutes and 4% fixation for 20 minutes at room temperature followed by bleaching overnight and treated with proteinase K (1 µg/ml, Roche, 03115844001). After 2 hours of blocking with 1% BSA ...
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bioRxiv - Immunology 2020Quote: Skin biopsy specimens were collected and minced before digestion in 1mL of DMEM with 1 mg/ mL collagenase D (Roche Diagnostics) for 3 hours at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: Cos-1 cells were grown in DMEM supplemented with 10% heat inactivated FCS and transfected with Fugene HD (Roche Applied Science) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... 2 mg/ml bovine serum albumin dissolved in TBST) for 1 h and subsequently incubated with anti-DIG-Fab antibody coupled to alkaline phosphatase (Roche: 11093274910) in blocking solution (1 in 5000 ...
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bioRxiv - Genetics 2019Quote: ... 10mM KCl, 1mM EDTA, 0.1mM activated Na3VO4, 0.5% NP-40, 10% glycerol, 1mM DTT and 1:1000 Roche cOmplete protease inhibitor) after the third wash ...
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bioRxiv - Genomics 2021Quote: ... Pelleted cells were resuspended in 1 ml of Dig-Wash buffer (0.02% digitonin, 20 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 1 Roche Complete tablet -EDTA (11873580001) per 50 ml buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... Riboprobes were hybridized for 16 hr at 65°C and embryos were incubated with anti-DIG-AP (Roche, dilution 1/4000) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... 1 MM EDTA) supplemented with a cocktail of protease inhibitors (Complete Protease Inhibitor without EDTA, Roche Applied Science, Indianapolis, IN, USA) and phosphatase inhibitors (Phosphatase Inhibitor Cocktail 3 ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were incubated with 1:10 TdT buffer for 10 min and subsequently in reaction mixture [0.12% v/v TdT (3333574001, Roche applied science); 0.25% v/v dUTP (11093070910 ...
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bioRxiv - Microbiology 2022Quote: ... amplification was performed in a 20-μl reaction mixture containing 1× Light Cycler Fast Start DNA master hybridization probes (Roche Diagnostics), 4 mM MgCl2 ...
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bioRxiv - Developmental Biology 2022Quote: ... at 15-20k cells/ well in Cardiac Mesoderm Patterning Media One made up of CDM containing ROCKi and for all protocols besides the LV cardioids 1 µg/ml of insulin (Roche, #11376497001) plus specific factors depending on cardioid subtype (see below) ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were rinsed with PBS and incubated with 5% (v/v) normal donkey serum and 1% (w/v) BSA (Roche, 10.7350.86001) in PBS for 1 hour at room temperature to block the non-specific antibody binding ...
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bioRxiv - Bioengineering 2022Quote: ... The optimal cycle numbers for PCR2 were determined for 1 μL of each PCR1 individually by conducting a qPCR amplification using KAPA HiFi HotStart Ready Mix (Roche, 7958927001) and 1× EvaGreen (Biotium ...
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bioRxiv - Bioengineering 2022Quote: ... Encapsulated or adherent cells were plated in a 96 well plate for 24 or 48h when WST-1 reagent (Roche, Switzerland) was added ...
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bioRxiv - Cell Biology 2022Quote: ... Pellets were washed in PBS and resuspended in lysis solution (1xPBS pH 7.4, 1x Roche plant protease Inhibitor, 1 mM PMSF) to a final concentration of 5x108 cells/mL ...
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bioRxiv - Microbiology 2022Quote: ... and cells were lysed on the plates for 30 minutes at 4ºC in Lysis Buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.5% NP-40, PhosStop, Roche Complete Protease Inhibitor). Lysates were clarified by centrifugation at 13,000 RPM ...
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bioRxiv - Microbiology 2022Quote: ... The total HIV-1 DNA was amplified by quantitative real-time PCR using the Light Cycler instrument (Roche Diagnostics, Meylan, France). Amplification was performed in a 20 μl reaction mixture containing 1× Light Cycler Fast Start DNA master hybridization probes (Roche Diagnostics) ...
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bioRxiv - Microbiology 2022Quote: ... Amplification was performed in a 20 μl reaction mixture containing 1× Light Cycler Fast Start DNA master hybridization probes (Roche Diagnostics), 3 mM MgCl2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Cells were lysed with tissue lysis buffer supplemented with 0.2 mM sodium vanadate and 1 mM protease inhibitor cocktail (Roche, Basel, Switzerland), and for immunoblotting ...
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bioRxiv - Molecular Biology 2023Quote: ... total RNA (50 μg) was separated on 1% agarose–formaldehyde gels and transferred to Hybond-N+ nylon membranes (Roche, Basel, Switzerland). Northern blotting was conducted with biotin-labeled DNA (bio-CTGTAGAAAGTCTGCTGATCGATACCGCGACG ...
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bioRxiv - Microbiology 2022Quote: ... and cells were lysed by resuspension in 10mL cold NP-40 lysis buffer (150mM NaCl, 10mM Tris pH 7.2, 1% NP-40, 5mM DTT, 2mM MgCl2) containing protease inhibitors (Roche, Laval, QC) on ice for 30 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... and the cell pellet resuspended in 1 ml of PBS with the addition of a protease inhibitor cocktail (cOmplete™ Protease Inhibitor Cocktail, Roche) in order to protect histones from degradation by proteases during the next step of cell lysis ...
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bioRxiv - Microbiology 2022Quote: Single cell suspensions from the ear were obtained by using tweezers to separate the dorsal and ventral sides of the mouse ear and placing the sheets dorsal-side down in a 24 well plate with 1 mL/well of RPMI1640 containing 0.25 mg/mL of Liberase TL (Roche, Diagnostics Corp.) and 10 μg/mL of DNase I (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with protease and phosphatase inhibitors (1 tablet per 5ml buffer of cOmplete™, mini, EDTA-free protease inhibitor cocktail [Roche] ...
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bioRxiv - Neuroscience 2022Quote: Protein was extracted from iNs by lysis in NP-40 lysis buffer (1% NP40, 0.5M EDTA, 5M NaCl, 1M Tris) containing cOmplete protease inhibitors and phosSTOP (Roche, Penzberg, Germany). Lysates were analyzed using the Multi-Spot Phospho (Thr 231)/Total Tau ELISA (Meso Scale Discovery ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclear pellets were suspended in RIPA buffer (50 mM Tris-HCl, 150 mM NaCl, 1% Nonidet P-40, 0.5% NaDeoxycholate, 0.1% SDS, 1x Roche protease inhibitor mixture) and sonicated 5 times x 5 min (30 s ON/ 30 s OFF ...