Labshake search
Citations for Roche :
8251 - 8300 of 10000+ citations for Cow Caprin 1 CAPRIN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... and ceramic beads (Precellys Lysing Kit CkMix, Precellys) in lysis buffer (Lysis Buffer 1X, Milliplex) to which a cocktail of protease inhibitors (Roche Complete Mini) and phosphatase inhibitors (PhosSTOP EASYpack ...
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bioRxiv - Cancer Biology 2023Quote: ... 300 µL of the aqueous phase was mixed with 375 µL ethanol and loaded onto a column from the hiPure miRNA purification kit (Roche, Cat. 5080576001). The samples were then washed and eluted according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: Apoptotic cells present in gonadal histological preparations were revealed by the terminal deoxyribonucleotidyl transferase (TDT)-mediated dUTP-digoxigenin nick end labelling (TUNEL) assay using the Fluorescent In Situ Cell Death Detection Kit (Roche ref. 11684795910, Roche, Mannheim, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: The TUNEL assay was performed to detect apoptosis using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and In-Situ cell death detection Fluorescein kit (Roche Cat#11684795910), as published ...
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bioRxiv - Genomics 2023Quote: Approximately 500ng Total RNA was used for RNA-seq library preparation by following the KAPA Stranded RNA-Seq Kit with RiboErase (HMR) sample preparation guide (Cat. No: KR1151, KAPA Biosystems, USA). The first step in the workflow involved the depletion of rRNA by hybridization of complementary DNA oligonucleotides ...
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bioRxiv - Immunology 2023Quote: Min6.luc and Min6.CD44 cells were harvested for total RNA isolation using the High Pure RNA isolation kit (Roche Applied Science) and reverse-transcribed using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time quantitative PCR was performed using gene-specific qPCR primers listed in Supplementary Materials and the KAPA SYBR Fast qPCR Master Mix (2X) kit (Kapa Biosystems, #KK4602), and ran on CFX96 Real-Time PCR Detection System (BIO-RAD ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Whole-genome libraries were prepared from sheared samples using the KAPA Hyper Plus library preparation kit (KK8514, Roche Diagnostics, Santa Clara, California), NEBNext Multiplex Oligos for Illumina (E7600 ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was synthesized by a reverse transcriptase reaction with 500 ng of total RNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche, Basal, Switzerland), and was used as a template for qPCR with LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Microbiology 2023Quote: ... a 710 bp region was amplified by PCR using previously described primers (45) and the KAPA HiFi HotStart ReadyMix PCR Kit (Roche, Basel, Switzerland) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Average library size and quality control were performed using a Fragment Analyzer (High Sensitivity NGS kit) and qPCR (Roche Light Cycler 480). CUT&RUN libraries were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA samples were submitted to the Dana-Farber Molecular Biology Core Facility for mRNA sequencing using the Roche Kapa mRNA Hyper Prep kit (Roche, Basel, Switzerland) with samples sequenced on an Illumina NextSeq or NovaSeq ...
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bioRxiv - Physiology 2023Quote: ... 500ng of total purified RNA was used for library preparation with the KAPA RNA HyperPrep Kit with RiboErase (HMR) for Illumina Platforms (KAPA Biosystems KK8561) according to the manufacturers protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared by the Van Andel Institute Genomics Core from 500 ng of total RNA using the KAPA stranded mRNA kit (v4.16) (Kapa Biosystems, Wilmington, MA). RNA was sheared to 300-400 bp ...
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bioRxiv - Physiology 2024Quote: ... RNA probes were synthesised and labelled using the T7-ScribeTM Standard RNA IVT Kit (Madison, Wisconsin, United States) and Digoxigenin-11-UTP (Roche, Basel, Switzerland). Embryos at various stages were fixed in 4% paraformaldehyde overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... For this 2 µl of RNA sample were mixed with the PCR ingredients of the one-step LightCycler 480 RNA Master Hydrolysis Probes kit (Roche Holding AG), the JFH1-specific probe (5’-6FAM-AAA GGA CCC AGT CTT CCC GGC AA-TMR-3’ ...
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bioRxiv - Microbiology 2023Quote: One microgram of RNA of each isolate was converted into cDNA using the Transcriptor high-fidelity cDNA synthesis kit (Roche, Basel, Switzerland). RT-PCR was performed in 96-well plates using the PowerUp SYBR green master mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... The concentration of ligated fragments in each library was quantified with the KAPA Library Quantification Kits for Illumina platforms (Roche/KAPA Biosystems, KK4824) on a Roche LightCycler 480 Instrument (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: ... The bulk transcriptome of these cases was obtained after macrodissection of the whole tumor area on the same block as the one used for the IHC and obtained from FFPE sections using a high-purity FFPE RNA isolation kit (Roche, Basel, Switzerland) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: Cytotoxicity was assessed by measuring the release of lactate dehydrogenase (LDH) into the culture supernatant using the cytotoxicity detection kit PLUS (Roche, Basel, Switzerland). 100 μl cell-free supernatant ...
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bioRxiv - Microbiology 2023Quote: Transcriptional start sites (TSS) of rokL6 and sco1448 were determined by 5’ RACE using a 5’/3’ RACE Kit 2nd generation (Roche, California, US). Total RNA (2 μg ...
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bioRxiv - Biochemistry 2023Quote: ... Final quantification of the RNA-seq library was done with the Library Quantification Kit for Illumina® platforms (KAPA Biosystems, M.A, U.S.A.). The C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The primary antibody was either visualized with the multimer-technology based UltraView Universal DAB Detection kit (Ventana® BenchMark® XT; Roche) or a fluorochrome-labeled secondary antibody (Alexaconjugated ...
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bioRxiv - Pathology 2023Quote: Cell death was assessed in tissue sections using terminal deoxynucleotidyl transferase-mediated dUTP nick- end labeling (TUNEL, Roche TUNEL kit (12156792910; Roche, Basel, Switzerland), as previously described (Cade et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Deletion of FGSG_09786 was further verified by Southern blot with the Digoxigenin High Prime DNA Labeling and Detection Starter Kit I (Roche, Mannheim, Germany). Using a similar strategy ...
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR analysis was carried out using the qScript One-Step PCR kit (Quanta Biosciences, Gaithersburg, MD) on a LightCycler 96 System (Roche, Indianapolis, IN). Fold-change values were calculated using the 2-ΔΔCt method ...
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bioRxiv - Evolutionary Biology 2023Quote: The Illumina library was prepared with 250 ng of unsheared DNA using the Kapa Hyperplus Library Preparation Kit with only one third of the volume reactions as described in the manufacturer’s protocol (Kapa Biosystems, Wilmington, MA). The total fragmentation volume was 16.66 μl and was incubated at 37°C for 7:45 min ...
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bioRxiv - Microbiology 2024Quote: ... while supernatants from enriched soil/water samples and rectal swabs were extracted using the High Pure PCR Template Preparation kit (Roche, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Both DNA samples were also converted into Illumina sequencing libraries with the KAPA HyperPrep library construction kit (Roche Sequencing Solutions, Indianapolis, IN) and paired-end sequenced for 150 cycles on a NovaSeq6000 (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche cat# KK4824) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Neuroscience 2024Quote: ... Accurate quantification of the final libraries for sequencing applications was determined using the qPCR-based KAPA Biosystems Library Quantification kit (Kapa Biosystems, Inc.). Each library was diluted to a final concentration of 1.4 nM and pooled equimolar prior to clustering ...
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bioRxiv - Neuroscience 2024Quote: ... A DNA library targeting polyadenylated transcripts was constructed for each sample in a 12-cycle PCR (100ng total RNA, KAPA hyper mRNA stranded library prep kit, Roche, catalogue# KK8581). Final cDNA libraries were checked for quality by TapeStation and qPCR (Kapa’s library quantification kit for Illumina Sequencing platforms ...
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bioRxiv - Neuroscience 2024Quote: ... Final cDNA libraries were checked for quality by TapeStation and qPCR (Kapa’s library quantification kit for Illumina Sequencing platforms, catalogue# KK4835, Kapa Biosystems, Wilmington MA). Samples were clustered and sequenced using NovaSeq S2 reagents (NovaSeq S2 Run ...
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bioRxiv - Neuroscience 2024Quote: ... DNA was isolated from tail clips taken from neonatal mice during sacrifice using the Kapa Express Extract Kit (Kapa Biosystems, Cat. #KK7100) with one minor modification – sample lysis was assisted via physical homogenization using a 1.5 mL tube plastic pestle ...
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bioRxiv - Evolutionary Biology 2024Quote: A PCR free Illumina library (based on the same HMW extraction) was prepared using the Kapa Hyper Prep Kit (Roche, Basel, Switzerland), following manufacturer instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The relative expression of genes in leaves was determined by reverse transcription quantitative PCR performed on an Applied Biosystems StepOne Real-Time PCR System with KAPA SYBR® FAST qPCR Master Mix (2X) Kit (Kapa Biosystems) according to the protocol of the manufacturer ...
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bioRxiv - Plant Biology 2020Quote: ... 1mM Phenylmethylsulfonyl fluoride) supplemented with 1 x plant protease inhibitor cocktail (Complete EDTA-free, Roche) and 1 x phosphatase inhibitor cocktail (PhosSTOP ...
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bioRxiv - Plant Biology 2020Quote: ... 1mM Phenylmethylsulfonyl fluoride) supplemented with 1 x plant protease inhibitor cocktail (Complete EDTA-free, Roche). Cell lysates were centrifuged at 12,000 x g for 15min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... 75 U benzonase and 1 Complete Mini EDTA-free protease inhibitor tablet (Roche Applied Science). Insoluble proteins were removed by centrifugation twice at 20,000 x g for 20 min each ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM DTT) Supplemented with mini complete protease inhibitor cocktail tablet (Roche Diagnostics, Mannheim, Germany) (1) ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were blocked for 1 hr at room temperature (RT) in 3% BSA (Roche; 10735086001), 0.05% Tween (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... was used for staining Cse4-GFP and rat anti-HA (1:200 dilution: Roche, 11867423001) for Mad2-3HA staining as primary antibodies ...
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bioRxiv - Microbiology 2020Quote: ... Exponential phase cultures were inoculated 1:40 into 7H9 supplemented with kanamycin (Roche, 20ug/ml) and ATc (Sigma 100ng/ml ...
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bioRxiv - Neuroscience 2021Quote: ... DIG-labelled Arc riboprobe signal was amplified with anti-DIG-POD (1:300; Roche Diagnostics), Tyramide Signal Amplification (TSA ...
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bioRxiv - Developmental Biology 2020Quote: ... probes were detected with anti-digoxigenin Fab fragments conjugated with alkaline-phosphatase (1:2000, [Roche]). 5-Bromo-4-chloro-3-indolyl phosphate (BCIP ...
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bioRxiv - Genomics 2020Quote: ... Cells were washed in 2X PIC (Roche mini-tabs, 1 tab in 5 ml = 2X) and stored at -80°C until needed.
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bioRxiv - Microbiology 2022Quote: ... Pub-NS(1F) or Pub-NS(5F) using 1 µL of X- tremeGENE HP (Roche) and incubated for 3 h before refreshing the medium ...
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bioRxiv - Immunology 2021Quote: We used DOTAP (N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate) (Roche) to introduce LPS or oxPLs into cells following the method by Zanoni et al (Zanoni et al. ...
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bioRxiv - Cell Biology 2019Quote: Reverse transcription was performed from 1 µg of total RNA using Expand reverse transcriptase (Roche) and oligo dT or random hexamer oligonucleotides as a primer ...
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bioRxiv - Developmental Biology 2019Quote: ... These fragments were pooled and dissociated using 1:100 Liberase™ (2.5mg/ml, Roche 5401135001) in CMFB at 30°C with gentle agitation at 300 rpm for 30 minutes on a ThermoMixer™ Eppendorf Mixer ...