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Citations for Roche :
8151 - 8200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... microvessels obtained in the pellet were combined into a new conical sterile tube and further digested with collagenase-dispase (1 mg/ml, Roche) and DNase I (6.7 μg/ml ...
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bioRxiv - Microbiology 2023Quote: ... were ligated to the individual cDNA products to barcode the samples and Kapa HiFi HotStart Library Amplification kit (kk2612, Roche Sequencing and Life Science) was used for library amplification.
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bioRxiv - Neuroscience 2023Quote: ... and Digoxygenin (DIG) labeled dNTP’s (Roche), the resultant RNA cyp19a1 probe was then used for ISH.
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bioRxiv - Neuroscience 2023Quote: ... 0,5×106 cells were lysed in RIPA buffer containing cOmplete Protease Inhibitor cocktail (Roche). Protein extraction and Western blotting is performed as previously described [109] ...
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bioRxiv - Neuroscience 2023Quote: ... and BCIP (94 mg /ml; Roche) for 12-16 hours at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 5mM Levamisole (Tetramisole)) with NBT (37.5 mg /ml; Roche) and BCIP (94 mg /ml ...
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bioRxiv - Neuroscience 2023Quote: ... Anti-DIG antibody fragments conjugated with alkaline phosphatase (Roche; 1: 5000) were diluted in MABT ...
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bioRxiv - Microbiology 2023Quote: ... Virus stocks were made using HEK-293/17 cells that were transfected with 5 μg of viral DNA using X-tremeGENE HP (Roche, Basel, Switzerland) after 48 h incubation ...
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bioRxiv - Microbiology 2023Quote: ... PCR was performed using the Expand High Fidelity PCR System dNTPack (Roche Diagnostics GmbH, Mannheim, Germany).
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bioRxiv - Neuroscience 2023Quote: ... and 1× cOmplete EDTA-free protease inhibitor (Roche). Lysis was allowed on ice for 30min ...
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bioRxiv - Neuroscience 2023Quote: ... Pellets were then dissolved in RIPA buffer with 1× Phos-STOP (Roche) and 1× cOmplete EDTA-free protease inhibitor (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... Pellets were resuspended in lysis buffer (20mM Tris-HCL pH 8.0, 300mM NaCl; 4ml/100ml culture) with freshly added protease inhibitors (Roche #11697498001: 1 tablet/50ml) and lysozyme (0.1mg/ml) ...
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bioRxiv - Neuroscience 2023Quote: ... protease inhibitor (Roche; 11836153001) and phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... and phosphatase inhibitor cocktail (Roche; 4906837001) and quantified using a Pierce™ BCA protein assay (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... RNA was reverse transcribed into complementary DNA (cDNA) using Transcriptor reverse transcriptase kit (Roche Diagnostics, UK). qRT-PCR was carried out for Spy0107 ...
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bioRxiv - Microbiology 2023Quote: ... 0.5% Triton X100 and protease inhibitor cocktail (Roche), pH 8 ...
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bioRxiv - Microbiology 2023Quote: ... as well as by quantitative PCR (KAPA Biosystems, Wilmington, MA, USA). DNA libraries were multiplexed and loaded on an Illumina MiSeq instrument according to manufacturer’s instructions (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... qPCR and qRT-PCR was performed using LightCycler 480 SYBR Green I Master (Roche). The thermal cycling conditions were 95°C for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... anti-DIG antibody (Anti-Digoxigenin-AP Fab fragments) and Chemiluminescent detection with CSPD following the instructions of the kit (all products and kits from ROCHE).
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bioRxiv - Microbiology 2023Quote: ... and reverse transcripted using the Transcriptor First Strain cDNA Synthesis Kit (Roche).
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bioRxiv - Neuroscience 2023Quote: ... Glutamine quantifications were measured using Cedex Bio analyser 7100 (Roche), and concentrations were normalized by the protein content of each sample (mmol/L/µg).
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bioRxiv - Microbiology 2023Quote: ... 0.1% SDS) both supplemented with 1X EDTA-free protease inhibitor cocktail (#04693132001, Roche). For the two latter lysis conditions lysates were complemented with XT sample buffer (#1610791 ...
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bioRxiv - Microbiology 2023Quote: ... 1% NP-40, 1 mM EDTA, 1 mM EGTA, 0.1% SDS, 1X EDTA-free protease inhibitor cocktail [#04693132001, Roche], and 0.5% sodium deoxycholate) and collected using a cell scraper ...
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bioRxiv - Microbiology 2023Quote: ... and sequenced on a Roche 454 GS20/FLX platform with Titanium chemistry (Roche, Branford, Connecticut). Flow-grams were processed with the 454 Basecalling pipeline (v2.5.3).
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bioRxiv - Microbiology 2023Quote: ... Each PCR reaction consisted of 12.5 μl KAPA 2x HiFi Master Mix (KAPA Biosystems, Wilmington, MA, USA), 0.5 μl of 10 μM forward primer ...
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bioRxiv - Microbiology 2023Quote: ... and a KAPA qPCR library quantification kit (Roche Sequencing, Pleasanton, CA, USA). Confirmation of the amplified region size (bp ...
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bioRxiv - Microbiology 2023Quote: We used genomic DNA extracted by physical disruption for shotgun library construction with a KAPA Hyper Prep kit for Illumina (KAPA Biosystems)69 ...
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bioRxiv - Microbiology 2023Quote: ... the specified primer concentrations and 2X SYBR Green I Master Mix solution (LightCycler® 480 SYBR Green I Master, Roche). Standard curves were generated from known concentrations of 10-fold serial diluted DNA from B ...
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bioRxiv - Microbiology 2023Quote: ... protease inhibitor cocktail (cOmplete, Mini, EDTA-free, Roche)) and treated with 3 × 45 s bead beating with glass beads (particle size ≤106 μm ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions with a total volume of 25 μL included: 2× KAPA HiFi Hot Start Ready Mix (KAPA Biosystems), a final concentration of 0.1 mM for each primer and 7.5 μL of DNA sample ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 20 ml PBS plus two cOmplete Mini EDTA- free protease inhibitor tablets (Roche) and 25 μg/ml phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Microbiology 2023Quote: ... We recircularized linearized plasmid PCR products with a Rapid DNA Ligation Kit (Roche) and transformed plasmids into DH5α-competent cells (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... 1 mM EDTA, 0.1 % NP-40, 150 mM NaCl, 0.5 mM DTT, plus a protease inhibitor cocktail from Roche; used in pull-down experiments with the GFP-Trap resin of Proteintech) ...
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bioRxiv - Microbiology 2023Quote: ... GFP- or HA3x-tagged proteins were detected using α-GFP (1:1000; Roche) or α-HA3x (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with a protease inhibitor cocktail (Complete, Roche)) ...
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bioRxiv - Microbiology 2023Quote: Frozen cells were thawed and suspended in 50 mM Tris-HCl (pH 7.5) supplemented with a few crystals of DNase I (Roche) and protease inhibitors (PI ...
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bioRxiv - Microbiology 2023Quote: ... pH 4.3) containing two dissolved cOmpleteTM EDTA-free protease inhibitor cocktail tablets (Roche Applied Science, Penzberg, Germany) and vacuum-infiltrated at 900 mbar for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... and protease inhibitors (PI) (cOmplete mini, EDTA-free, Roche). To ensure anaerobic conditions ...
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bioRxiv - Microbiology 2023Quote: ... and 1X complete protease inhibitor (Roche) were added to the cell lysate and incubated on ice for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... TUNEL detection Kit (TUNEL488 probe, Roche) & DAPI (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... harvested by centrifugation and resuspended in 0.3 ml PBS supplemented with complete mini protease Inhibitor Cocktail (Roche). Whole cell protein extracts were obtained as described above and protein concentrations determined using Bradford reagent (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... washed one time with 1 ml phosphate-buffered saline (PBS) and resuspended in 0.3 ml PBS supplemented with complete mini protease Inhibitor Cocktail (Roche). Cell suspensions were transferred to lysis tubes containing glass beads and subjected to mechanical disruption in a homogenizer SpeedMill Plus (Bioanalytik Jena ...
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bioRxiv - Microbiology 2023Quote: ... and 12.5 µl of KAPA HiFi HotStart ReadyMix (Kapa Biosystems; Wilmington, MA, USA). A second PCR was performed using dual Illumina indices as per the Illumina Nextera XT Index Kit ...
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bioRxiv - Microbiology 2023Quote: ... qPCR analysis was done on 100ng of DNA using a LightCycler 480 Probe Master-Mix kit (Roche, Cat. No. 04707494001) and a primer and probe set specific to MHV68 gB to quantify the number of viral genome copies ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time PCR was carried out using FastStart Universal SYBR Green Master Mix (Roche) on a CFX 384 Bio-Rad thermal cycler (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... and biotin RNA labeling mix (Roche 11685597910).
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bioRxiv - Molecular Biology 2023Quote: ... on a LightCycler 480 II (Roche, Mannheim, Germany), using manufacturer’s recommended thermocycling conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... Expand™ Long Template PCR System (Roche) was used with the following thermocycling program ...
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bioRxiv - Molecular Biology 2023Quote: ... were lysed by sonication in ∼140 mL lysis buffer (50 mM Tris-HCl pH 8, 250 mM KCl, 1 mM TCEP) supplemented with protease inhibitor cocktail (Roche) and DNase I (5 μg/ml ...
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bioRxiv - Microbiology 2023Quote: ... Cells were harvested in non-denaturing buffer (20 mM Tris, 500 mM NaCl, 20 mM Imidazole and protease inhibitor cocktail (Roche), pH 7.5 ...