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Citations for Roche :
7951 - 8000 of 8835 citations for FH1 FH2 domain containing protein 1 FHOD1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... rat colon was homogenized in lysis buffer using 1 mm glass beads (Thistle Scientific) in a MagnaLyser system (Roche). The samples were filtered through a binding column ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM TCEP and 10 % glycerol) supplemented with 2x EDTA-free protease inhibitor cocktail tablets (Roche, cat. No. 11836153001) and 4 μg/ml DNase I ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated by dissociating tumor tissue in the presence of collagenase D (1 mg/ml) (Roche-Merck) for 10 min at 37°C ...
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bioRxiv - Cell Biology 2022Quote: all the samples were lysed with High Salt Buffer (50 mM Tris pH 7.5, 300 mM NaCl, 1% Triton X-100, Protease inhibitors (Roche), Phosphatase inhibitors Halt cocktail ...
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bioRxiv - Genomics 2022Quote: ... 25 mM potassium chloride, 5 mM magnesium chloride, 10 mM Tris-HCl pH 8.0, 1 μM DTT, 1x Roche Protease Inhibitor Cocktail ...
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bioRxiv - Biochemistry 2022Quote: ... 1-2×108 cells were lysed by mechanical lysis with glass beads in native lysis buffer (150 mM Tris/HCl pH 7.5, 50 mM NaCl, 1 mM PMSF and Complete protease inhibitors (Roche)) ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.34 M Sucrose, 10% Glycerol, 1 mM DTT, 0.1% TritonX100 added with 1x PI and 1x Phosphatase Inhibitors - Roche) and left on ice for 8 min ...
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bioRxiv - Biophysics 2022Quote: ... culture medium was collected and cells were gently washed twice with 5 ml cold PBS and lysed in 0.5 ml of lysis buffer (PBS, 1% (v/v) Triton X-100 and protease inhibitor cocktail (Roche)) ...
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bioRxiv - Bioengineering 2022Quote: Mouse hearts were harvested from euthanized mice and enzymatically digested with collagenase A (1 mg/mL; Roche Life Science) and dispase (2.5 U/mL ...
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bioRxiv - Biochemistry 2022Quote: ... cells were harvested and solubilized in the lysis buffer (20 mM HEPES, pH 7.5, 150 mM NaCl, 10% glycerol) plus 1% LMNG and Protease Inhibitor Cocktail (Roche) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The bacterial pellet was resuspended in lysis buffer (7.5 mL PBS supplemented with 1% Triton, 10 μg/mL DNaseI, EDTA-free Protease Inhibitor Cocktail (Roche)) and was incubated for 1 h on ice ...
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bioRxiv - Cell Biology 2022Quote: ... The pellet was resuspended with 20 mL lysis buffer (50 mM NaH2PO4, 0.5 M NaCl, pH 8) and 1 cOmplete Mini Protease Inhibitor Cocktail tablet (Roche) per 1 L of expression ...
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bioRxiv - Cell Biology 2022Quote: H3K4me3-immunoprecipitated genomic DNA was diluted 1:100 and Chip-qPCR performed with a Light Cycler 480II machine (Roche) using technical duplicates and ChIP-qPCR signals were calculated as % of input ...
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bioRxiv - Genomics 2022Quote: NGS data from German field samples was analysed by mapping all reads against the ASFV Germany 2020/1 (LR899193) [18] genome sequence as reference using Newbler 3.0 (Roche) with default parameters including adapter and quality trimming ...
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bioRxiv - Genetics 2022Quote: ... resuspended in lysis buffer (25 mM Tris-HCl at pH 7.4, 150 mM NaCl, 10% glycerol, 1% NP-40, Roche Complete EDTA-free protease inhibitor cocktail ...
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bioRxiv - Developmental Biology 2022Quote: ... The probe was mixed in hybridization buffer (0.1 g/ml dextran sulfate [Sigma D8906-50G], 1 mg/ml Escherichia coli tRNA [ROCHE 10109541001] ...
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bioRxiv - Plant Biology 2022Quote: ... 1 µg of purified RNA was subjected to reverse transcription using a Transcriptor First Strand cDNA Synthesis Kit (Roche) and 1 µl of RNase A/T1 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: 7-day-old Q28 and Q69 seedlings were lysed in lysis buffer (1% Triton X-100, 50 mM Tris–HCl pH 8.0) supplemented with protease inhibitor cocktail (Roche) and 25 mM N-ethylmaleimide ...
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bioRxiv - Molecular Biology 2022Quote: ... 60 mM KCl, 15 mM NaCl, 1 mM CaCl2, 0.9% Triton X-100, and 13 protease inhibitor cocktail [Roche]). After homogenization ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM EDTA, 0.5% NP-40, 1 mM DTT, 1X protease inhibitor [EDTA-free complete Protease Inhibitor Cocktail, Roche, 11873580001] and 1:100 phosphatase inhibitor [Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... cell pellets were lysed in whole-cell extraction buffer (150 mM NaCl, 50 mM Tris pH 7.5, 1% NP-40) supplemented with proteinase and phosphatase inhibitors (Roche). The lysates were cleared by centrifugation at 14,000 rpm for 30 min at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... ground in a fine powder with liquid nitrogen and resuspended with 1 ml of Guanidine hydrochloride extraction buffer (6M GuaHCl, HEPES 1 M, EDTA: 5 Mm, adjusted to pH 7.5) supplemented with protease inhibitor cocktail (Roche). Homogenates were filtered through a 100 µm cotton mesh ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected according to the manufacturer’s protocol at a µL lipofectamine: µg plasmid ratio of 3:1 (X-tremeGENE 9, Roche). After 48 hours ...
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bioRxiv - Plant Biology 2023Quote: ... in 100μl GTEN-buffer (10% Glycerol, 25mM TRIS pH 8, 0.5mM EDTA, 150 mM NaCl,1 x protease inhibitors (Roche), 5mM DTT ...
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bioRxiv - Plant Biology 2023Quote: ... A GTEN-based buffer (10% Glycerol, 25mM TRIS pH 8, 0.5mM EDTA, 150 mM NaCl, 1 x protease inhibitors (Roche), 5mM DTT ...
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 1000 µL cold cytoplasmic lysis buffer (10 mM HEPES pH 7.9, 10 mM KCl, 2 mM Mg acetate, 3 mM CaCl2, 340 mM sucrose, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA to 1× ...
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bioRxiv - Microbiology 2023Quote: ... cells were resuspended in 1000 µL cold sucrose buffer containing (10 mM HEPES pH 7.9, 10 mM KCl, 2 mM Mg acetate, 3 mM CaCl2, 340 mM sucrose, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA to 1× ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were collected as the cytoplasmic fraction and pellets were resuspended in 200 µL cold nuclear lysis buffer (20 mM HEPES pH 7.9, 100 mM NaCl, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA free ...
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bioRxiv - Plant Biology 2022Quote: ... and vortexed vigorously in extraction buffer 1 (50 mM Tris–HCl pH 7.5, 150 mM NaCl, Complete EDTA-free Protease Inhibitor cocktail (Roche), PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... rat cortical neurons cultured as described above were lysed directly in buffer (50 mM HEPES pH 7.0, 2% SDS, 1 mM EDTA plus protease inhibitor mixture (PIC, Roche) and 20 mM MMTS (to block free thiols) ...
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bioRxiv - Biochemistry 2023Quote: Cell pellets were snap frozen in Eppendorf tubes and lysed with glass beads in 500 μl GFP pull-down buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...
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bioRxiv - Biochemistry 2023Quote: Cell pellets were snap frozen in Eppendorf tubes and lysed with glass beads in 500 μl GFP pull-down buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free ...
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bioRxiv - Microbiology 2022Quote: ... Quantification of OsHV-1 and total bacteria were performed using quantitative PCR (qPCR, Roche LightCycler 480 Real-Time thermocycler) with the following program ...
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bioRxiv - Cell Biology 2023Quote: ... cardiomyocytes were dissociated using DPBS for 20 min at 37 °C followed by 1:200 Liberase TH (Roche, 5401151001) diluted in DPBS for 20 min at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... washed and resuspended in lysis buffer (50 mM Tris pH 7.5; 150 mM NaCl; 1 mM MgCl2; 10% Glycerol; Complete protease inhibitor (Roche); 1 mM PMSF or 2 mM AEBSF-hydrochloride (BioChemica APA1421.0001) ...
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bioRxiv - Biophysics 2022Quote: ... 5 EDTA and 1% Triton X-100) supplemented with cOmplete™ Mini-EDTA free protease inhibitor cocktail (Roche, Germany). Lysates were incubated 5 min on ice and centrifuged 10 min - 14000 x g at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting cDNA was analysed by qRT-PCR on a LightCycler 480 using SYBR Green 1 Master Mix (Roche). Relative fold change in expression was determined by the ddCt method ...
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bioRxiv - Genetics 2023Quote: Mouse tissue was homogenized in 400 μl of RIPA homogenizing buffer (50-mM Tris HCl pH 7.4, 150-nM NaCl, 1-mM EDTA, supplemented with protease-inhibitor cocktails (Roche)) and lysed with a MagNA Lyser ...
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bioRxiv - Immunology 2023Quote: 50,000 sorted cells were washed with PBS and resuspended in Buffer 1 (1X eBioscience Perm/Wash Buffer, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Immunology 2023Quote: ... and incubated on ice for 1 hr and washed twice with Buffer 2 (0.05% w/v Saponin, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Microbiology 2023Quote: ... released by trypsin and incubated in ice-cold lysis buffer (Tris-HCl pH 8.0, 50mM, NaCl 150mM, EDTA 1mM, NP40 1%, cOmplete Mini Protease Inhibitor Cocktail (Roche) and phosphatase inhibitor PhosSTOP (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then released by trypsin and lysed in ice-cold lysis buffer (Tris-HCl pH 8.0, 50mM, NaCl 150mM, EDTA 1mM, NP40 1%, cOmplete Mini Protease Inhibitor Cocktail (Roche) and phosphatase inhibitor PhosSTOP (Roche) ...
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bioRxiv - Developmental Biology 2023Quote: ... Minced lung was enzymatically dissociated to a single cell suspension using 1 mg/mL collagenase A (Roche, Cat# 10103578001), 2-4U/mL elastase (Worthington ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001], PhosSTOP [Roche, PHOSS-RO] ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed 1x with PBS and lysed with lysis buffer (25 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001] ...
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bioRxiv - Plant Biology 2023Quote: ... 5% glycerol, 50mM KCl, 5 mM EDTA, 0.1% Triton X-100, 5 mM DTT and 1 × protease inhibitor cocktail [Roche]). The homogenized lysates were incubated at 4 °C for 1 hour for western blot analysis of eIFiso4G1 or overnight for mass spectrometry analyses with 50 µl of prewashed immobilized γ-aminophenyl-m7GTP (C10-spacer)-agarose beads (Jena Bioscience) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed in with glass beads in 500 µl SPOT PD buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% Glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed in with glass beads in 500 µl SPOT PD buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% Glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free ...
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bioRxiv - Immunology 2023Quote: ... enzymatic digestion of tissue pieces was done with collagenase D (1 mg/ml) and DNAseI (0.1 mg/ml) (both from Roche) in HBSS/10%FBS for 45 min (37°C ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended in lysis buffer (300 mM KCl, 1 mM MgCl2, 0.1 mM ATP, 1X Roche Protease/PPase inhibitor ...