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Citations for Roche :
751 - 800 of 7686 citations for rno mir 16 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... 16S rRNA genes were amplified using KAPA2G™ Robust HotStart ReadyMix PCR Kit (Kapa Biosystems, Wilmington, MA). Amplification conditions for fast PCR using the KAPA2G™ polymerase were as follows ...
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bioRxiv - Pathology 2020Quote: ... these protocols were adjusted to a real-time format with Green Essential FastStart Master kit (Roche Diagnostics), in a Nano LightCycler (Roche Diagnostics) ...
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bioRxiv - Cell Biology 2022Quote: ... Two different HRMA-compatible platforms were used (Applied Biosystems ViiA 7 Real-Time PCR System, using the MeltDoctor HRM Master Mix, and Roche LightCycler 480 System ...
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bioRxiv - Physiology 2019Quote: ... The reverse transcription product was diluted according to the efficiency curve and submitted in duplicates to real-time quantitative PCR using LightCycler® 480 SYBR Green I Master (Roche) in 7500 System (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... mgc2 adhesin and mgc2-like adhesin (list of primers used in Table 2) were made by real-time PCR (rtPCR) on LightCycler 96 thermocycler (Roche®). The LightCycler SYBR Green I Master reaction mix (Roche® ...
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bioRxiv - Neuroscience 2020Quote: ... Results were obtained with the Basic Relative Quantification Analysis of the LightCycler® 480 Real-Time PCR System software (Roche, Switzerland) and analyzed using the ΔΔCt method ...
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bioRxiv - Biochemistry 2021Quote: ... The expression of targeted genes and GAPDH transcripts were monitored by LightCycler®480 Real-time PCR (Roche Diagnostics GmbH, Germany) using gene-specific primers (Eurofins ...
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bioRxiv - Molecular Biology 2020Quote: ... for 40 cycles at 95°C for 5 s and 60°C for 30 s on a Light Cycler 480 Real-Time PCR System (Roche, Switzerland). All primer sequences used in this study are listed in Supplementary materials (Supplementary Table S1).
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was quantified by Real-Time PCR on Light Cycler II using Light Cycler 480 SYBR Green I Master (Roche, 04887352001).
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bioRxiv - Synthetic Biology 2022Quote: ... Total RNA (1 μg) was reverse transcribed into cDNA and then quantitative real-time PCR was performed using a LightCycler 96 System (Roche, Switzerland). Relative gene expression was calculated using the 2-ΔΔCt method after normalizing to GAPDH expression ...
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bioRxiv - Plant Biology 2021Quote: ... and the mRNA content was quantified by real-time PCR using the LightCycler® 96 System (Roche Diagnostics K.K., Tokyo, Japan) with a KAPA SYBR FAST One-Step qRT-PCR Kit (Nippon Genetics Co. ...
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bioRxiv - Physiology 2021Quote: ... The expression of targeted genes and GAPDH transcripts were monitored by LightCycler®480 Real-time PCR (Roche Diagnostics GmbH, Germany) using gene-specific primers (Eurofins ...
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bioRxiv - Immunology 2021Quote: ... The transcripts for genes of interest were measured by real-time PCR with a Lightcycler 480 II system (Roche, Basel, Switzerland) and a Brilliant III SYBR Green Master mix (Agilent Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted DNA was quantified by real-time PCR on a Roche LightCycler by using FastStart Universal SYBR Green Master (Roche, #4913850001) with the set of primers listed in Supplementary Table 4 ...
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bioRxiv - Microbiology 2022Quote: ... The total HIV-1 DNA was amplified by quantitative real-time PCR using the Light Cycler instrument (Roche Diagnostics, Meylan, France). Amplification was performed in a 20 μl reaction mixture containing 1× Light Cycler Fast Start DNA master hybridization probes (Roche Diagnostics) ...
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bioRxiv - Plant Biology 2022Quote: Real-time PCR was performed using the HS-qPCR SYBR Blue (2x) (Biolabmix, Russia) on a LightCycler®96 (Roche, Germany). qPCR was carried out in three biological and three technical replicates ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 ug of the total RNA was reverse transcribed into cDNA and the quantitative real-time PCR was performed using a LightCycler 96 System (Roche, Switzerland). Relative gene expression was calculated using the 2−ΔΔCt method by normalizing it to GAPDH expression ...
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bioRxiv - Plant Biology 2023Quote: ... The expression patterns of candidate genes were explored by Real-time fluorescence quantitative PCR using a Roche thermocycler (LightCycler480, Roche, Switzerland). The ACTIN2 (At3g18780 ...
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bioRxiv - Cancer Biology 2023Quote: ... HSJD-DIPG-012 and HSJD-DIPG-014 cell lines by real-time quantitative QRT-PCR on a LightCycler® 480 instrument (Roche) using a RT² Profiler PCR Array (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... RT-qPCR was performed in duplicate for each sample using the LightCycler 480 Real-Time PCR System (F. Hoffmann-La Roche, Ltd.) with the following conditions ...
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bioRxiv - Neuroscience 2024Quote: ... titer was determined at a concentration of 1.1013 gcp/ml by quantitative real-time PCR using the Light Cycler 480 SYBR green master mix (Roche, cat # 04887352001) with primers specific for the AAV2 ITRs (fwd 50-GGAACCCCTAGTGATG GAGTT-3’ ...
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bioRxiv - Cell Biology 2020Quote: ... through the xCELLigence Real-Time Cell Analysis (RTCA; Roche Diagnostics) system ...
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bioRxiv - Plant Biology 2021Quote: ... Real Time qPCR was done in a Lightcycler 480 (Roche), using Lightcycler 480 Master I Mix (Roche) ...
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bioRxiv - Plant Biology 2023Quote: ... Real-time qPCR was done in a Lightcycler 480 (Roche) using the Lightcycler 480 SYBR Green I Master mix (Roche) ...
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bioRxiv - Immunology 2023Quote: ... One step real time RT-PCR was performed using 100 ng of total RNA and KAPA SYBR FAST One-Step qRT-PCR Kits (Kapa Biosystems) according to the directions provided by the manufacturer ...
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bioRxiv - Plant Biology 2021Quote: ... with chemiluminescent detection using DIG Luminescent Detection Kit (Roche, Switzerland).
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bioRxiv - Molecular Biology 2024Quote: ... The detection was done using DIG Luminiscent Detection Kit (Roche) following the recommendations of the manufacturer ...
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bioRxiv - Immunology 2022Quote: ... RT-PCR was performed using the LightCycler Multiplex RNA Virus Master Kit (Roche Diagnostics, Mannheim, Germany) on a Cobas z480 analyzer (Roche Diagnostics ...
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bioRxiv - Genomics 2020Quote: ... Reverse transcribed cDNA was then assayed for RT-qPCR using Sybr green PCR kit (KAPA biosystems) at 60°C melting temperature and quantitated using Biorad quantitative PCR system ...
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bioRxiv - Microbiology 2019Quote: RT-PCR was performed using LightCycler® RNA Amplification Kit SYBR Green (Roche Diagnostics Ltd., UK). The primers were (csf2-fw ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was obtained after reverse transcription using a First strand cDNA synthesis RT-PCR kit(Roche) and quantitative PCR was performed using SYBR Green (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was conducted on a LightCycler 480 II Detection System (Roche, Switzerland) with TransStar Tip Top Green qPCR Supermix (Transgen Biotech ...
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bioRxiv - Immunology 2022Quote: Cytotoxicity Detection Kit (Roche CustomBiotech), which quantifies cytotoxicity/cytolysis based on the measurement of lactate dehydrogenase (LDH ...
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bioRxiv - Microbiology 2019Quote: ... PCR targeting the V4 region of archaeal 16S rRNA genes was conducted with KAPA HiFi HotStart PCR kit (KAPA Biosystems, Cape Town, South Africa) and barcoded primer sets Arc519F/806R (Table S1) ...
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bioRxiv - Plant Biology 2019Quote: ... The blots were hybridized with probes that were labeled using PCR DIG Probe Synthesis Kit and hybridization was detected with DIG Luminescent Detection Kit following the manufacturer’s instructions (Roche, Switzerland).
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bioRxiv - Microbiology 2024Quote: Quantitative RT-PCR (RT-qPCR) was performed using SYBR green master mix (Roche, Indianapolis, IN) and a Light Cycler 480 Instrument II (Roche) ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction was performed in 96-well plates with a final volume of 20 μl.The thermal melting curve were monitored using a LightCycler 480 II Real-Time PCR System (Roche Diagnostics, Rotkreuz, Switzerland) with a ramp rate of 1 °C at the temperature range from 25 °C to 80 °C ...
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bioRxiv - Neuroscience 2020Quote: Expression of UPR marker and CDNF mRNAs was quantified using the LightCycler® 480 Real-Time PCR System and Lightcycler 480 SYBR Green I master mix (Roche, Switzerland). β-actin was chosen as a reference gene ...
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bioRxiv - Microbiology 2020Quote: Quantification of precipitated DNA was determined using real-time quantitative PCR (qPCR) with SYBER Green in FastStart Essential DNA Green Master (Roche, Basel, Switzerland). Each resultant DNA was diluted in nuclease-free water and was analyzed in triplicated for EBV-associated genes and CTCF binding sites ...
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bioRxiv - Epidemiology 2020Quote: ... plasmids or DNA samples (Table 1) by real-time TaqMan PCRs assays on a LightCycler® 480 (LC480) (Roche Applied Science, Germany). Real-time PCR assays were performed with LightCycler® 480 Probe Master Mix 1× (Roche Applied Science ...
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bioRxiv - Cancer Biology 2019Quote: ... The primers used for real-time PCR were designed based on the Universal Probe Library (Roche, Roche Life Science, Pleasanton, CA, USA) with GAPDH as reference ...
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bioRxiv - Neuroscience 2020Quote: ... Real-time semiquantitative PCR with TaqMan and SYBRGreen were performed in the LightCycler 480 Systems (384-well format, Roche Applied Science, Germany) with a reaction mix prepared to the final volume of 10 µL ...
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bioRxiv - Pathology 2020Quote: ... The specific pairs of primers used for the real-time PCR are listed in Supplementary Table 4 (designed by Universal ProbeLibrary Assay Design Center tool from Roche, Indianapolis, IN). The real-time PCR was performed with FastStart Universal Probe Master mix ...
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bioRxiv - Microbiology 2021Quote: ... A single sample (one mouse) was assessed on each array plate on a Roche Light Cycler 480 II real time PCR thermal cycler (Roche, Basel, Switzerland). All data were analyzed with Qiagen’s Gene Globe online analysis application ...
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bioRxiv - Immunology 2020Quote: ... pursued by 95°C for 3 minutes and then 45 cycles at 95°C for 15 seconds and 58°C for 30 seconds using a LightCycler 480 Real-Time PCR system (Roche, Rotkreuz, Switzerland). The primers and the probes were designed against the E gene (20).
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bioRxiv - Physiology 2023Quote: ... The cDNA was analyzed using real-time PCR with THUNDERBIRD SYBR qPCR Mix (TOYOBO) and a LightCycler 96 instrument (Roche, Basel, Switzerland), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The expression levels of individual genes of interest were determined by quantitative real time PCR (qPCR, Roche Diagnostics, Germany or Stratagen, USA), primer pairs used for amplification are listed in Table 1 ...
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bioRxiv - Developmental Biology 2019Quote: ... Quantitative RT-PCR (Q-PCR) was performed using a LightCycler 480 II (Roche) and LightCycler DNA Master SYBR Green I detection (Roche ...
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bioRxiv - Genetics 2021Quote: ... RT-PCR samples were analyzed using qPCR with SYBR green PCR mastermix (Roche), SYBR Premix Ex Taq II (Tli RNase H Plus ...
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bioRxiv - Microbiology 2021Quote: ... and RT-PCR was performed using Taqman reagents (Roche) using a StepOnePlus (ThermoFisher) ...