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Citations for Roche :
751 - 767 of 767 citations for SARS Coronavirus Spike Glycoprotein S2 Mosaic C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... The Block C and Block O blocking oligos were replaced with an equivalent volume of SeqCap EZ Developer Reagent (Roche Diagnostics, Mannheim, Germany). Each reaction was amplified for 16 cycles ...
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bioRxiv - Bioengineering 2022Quote: ... Fetal livers were cut in small fragments and then treated for 45 min at 37°C with collagenase D (Roche; 100 ng/ml), and a cell suspension was prepared ...
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bioRxiv - Immunology 2023Quote: ... a left lobe was digested for 1 hour at 37°C in RPMI containing 13 mg/mL DNase I (Roche, Randburg, South Africa) and 50 U/mL collagenase IV (Gibco ...
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bioRxiv - Immunology 2023Quote: ... The gut was then added to a 1.5 mL Eppendorf tubed filled with 1 mL pre-warmed 37°C collagenase/dispase solution (Roche 10 269 638 001) and incubated for 90 seconds before adding to a 40-micron filter as above ...
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bioRxiv - Neuroscience 2024Quote: ... Oocytes were then incubated for 60 min at 4 °C with 100 μg/ml rat monoclonal anti-HA antibody (clone 3F10, Roche Pharmaceuticals, Basel, Switzerland) in 1% BSA/ND96 ...
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bioRxiv - Immunology 2021Quote: ... This lysate was incubated at 37 °C for 80 minutes and samples subsequently diluted in ice-cold DISC buffer (containing 2 mM NEM and Roche complete protease-inhibitor cocktail), cleared by centrifugation ...
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bioRxiv - Immunology 2020Quote: ... Lyzed cells were centrifuged at 20,000 x g for 30 min at 4°C and the supernatant was mixed with protease inhibitor (cOmplete ULTRA Tablets, Roche, Sigma-Aldrich, Cat: 05892953001). Protein concentration was determined using the Qubit Protein Assay Kit and Fluorometer (Invitrogen) ...
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bioRxiv - Developmental Biology 2020Quote: ... hybridized with sense or antisense probes overnight at 65 °C and incubated with the alkaline phosphatase-coupled sheep anti-digoxigenin antibody (1:2000, Roche Applied Science, Manheim, Germany) overnight at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: U2OS cells were seeded in conditioned medium in triplicate in 6 cm plates and allowed to attach for 4 h before treatment with mitomycin C (Roche 10 107 409 001) or olaparib (Selleckchem AZD2281 ...
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bioRxiv - Genomics 2020Quote: ... the tissues were cut into 1-2 mm2 pieces and put in 1mg/ml collagenase and dispase (C/D) solution (Roche Diagnostics GmbH Roche Applied Science, Mannheim, Germany) at 37°C for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... The KSHV genome-enriched CHi-C library DNA was eluted and PCR enrichment (12 cycles) performed with high-fidelity KAPA HiFi HotStart DNA Polymerase (Kapa Biosystems, Inc., Wilmington, MA). Libraries were multiplex sequencing (2 × 150 bp ...
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bioRxiv - Bioengineering 2023Quote: ... followed by 10 minutes at 37°C in a 3% (w/v) collagenase A solution in PBS (Collagenase A, Roche Diagnostics GmbH, Germany, 10103586001). Cells were recovered by centrifugation for 5 minutes at 300g prior cell counting on Malassez cells after Trypan blue staining ...
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bioRxiv - Neuroscience 2024Quote: ... resuspended in 150 μL buffer C (50 mM Tris pH 8, 5 mM EDTA, 1% SDS, 100 mM NaCl, 1x Roche complete mini protease inhibitors) and incubated on ice for 10 min ...
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bioRxiv - Molecular Biology 2019Quote: ... and coupled with 10 μg carrier-free anti-epitope tag antibody (c-myc: Santa Cruz #sc-40; FLAG: Sigma #F1804; GluGlu: Biolegend #901801; HA: Roche #11867423001; VSVG: Sigma #SAB4200695) in a total volume of 500 μL 50 mM MES (pH 5.0) ...
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bioRxiv - Immunology 2023Quote: ... mLNs were incubated at 37°C in digestion medium (RPMI-1640 supplemented with 0.8 mg/mL dispase, 0.2 mg/mL Collagenase P [Roche] and 0.1 mg/mL DNase I [Invitrogen]) and gently pipetted at 5-minute intervals to ensure complete dissociation of the tissue ...
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bioRxiv - Evolutionary Biology 2023Quote: ... mRNA was purified from approximately 200 ng of total RNA with oligo-dT beads and sheared by incubation at 94 C in the presence of Mg (Roche Kapa mRNA Hyper Prep Catalog # KR1352).
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bioRxiv - Immunology 2021Quote: Adipose and other tissues were removed from isolated thymi which were subsequently subjected to three rounds of enzymatic digestion for which cut up lobes were incubation at 37°C in Liberase digestion buffer (PBS Gibco 1X, Liberase Roche 2.5 mg/ml, DNase Roche 10 mg/ml). Cells were incubated with anti-CD45 beads (Miltenyi Biotec ...