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Citations for Roche :
451 - 500 of 767 citations for SARS Coronavirus Spike Glycoprotein S2 Mosaic C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The TUNEL reaction was performed in a humid chamber for 2.5 h at 37 °C with the in Situ Cell Death Detection Kit (Roche) using 100 μL of reaction mixture per slide ...
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bioRxiv - Microbiology 2021Quote: ... RNA samples were incubated for 30 min at 37°C with 20 U of RNase-free DNase I (Roche) in a final reaction volume of 30 μl ...
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Toxoplasma GRA15 limits parasite growth in IFNγ-activated fibroblasts through TRAF ubiquitin ligasesbioRxiv - Immunology 2020Quote: ... The membrane was blotted overnight at 4°C with rat antibody against the HA tag (Roche, 1/500 dilution), TRAF2 and TRAF6 rabbit antibodies (Suppl ...
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bioRxiv - Immunology 2022Quote: ... Collected tissue was mechanically dissociated by chopping followed by enzymatic digestion for 45 minutes at 37°C in DMEM containing collagenase D (1.5 mg/ml, Roche) and DNase I (60 U/ml ...
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bioRxiv - Immunology 2020Quote: ... minced with scissors and digested at 37°C for 20-30 minutes in digestion buffer (0.52 U/ml Liberase TM [Roche] and 60 U/ml DNase I [Roche] in RPMI 1640 [Cellgro] with 5% fetal bovine serum) ...
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bioRxiv - Cell Biology 2021Quote: ... at 37°C in a 0.4 μm filtered solution of 0.5 mg/mL collagenase D (Roche, Indianapolis, IN, USA), 1 Unit/mL Dispase (Stemcell Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... Crosslinks were reversed at 65°C overnight and the eluates were treated with RNase A and Proteinase K (Roche), prior to an extraction with phenol:chloroform:isoamyl alcohol ...
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bioRxiv - Genetics 2021Quote: ... Capture-C libraries were made using the Arima HiC kit (Arima Genomics) and the KAPA HyperPrep kit (KAPA Biosystems) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... CaCl2 was added to a final concentration of 1.25 mM and incubated with 200 μl Anti-Protein C Affinity Matrix (Roche) for 4 hrs while rotating at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... brains were dissected in a cold Ringer’s solution and dissociated by incubation with collagenase/dispase mix at 29°C (Roche, 15 minutes for larval and pupal brains and 30 minutes for adult brains) ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated overnight at 4°C in B1 buffer with alkaline phosphatase-conjugated anti-DIG (1/2000; Roche 11633716001). After three washes in B1 buffer and one wash in B3 buffer (100 mM Tris–HCl pH 9 ...
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bioRxiv - Genomics 2021Quote: ... Samples were then incubated overnight at 4°C with a rabbit anti-DIG HRP-conjugate antibody (1:500, Roche). Then ...
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bioRxiv - Synthetic Biology 2021Quote: ... 72 °C for 20 seconds] and acquired fluorescence after each cycle using a Lightcycler® 96 (Roche Life Science).
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bioRxiv - Synthetic Biology 2021Quote: ... 62 °C for 24 seconds] and acquired fluorescence after each cycle using a Lightcycler® 96 (Roche Life Science).
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bioRxiv - Developmental Biology 2020Quote: ... embryos were incubated overnight at 4°C with 1:2000 alkaline phosphatase-conjugated anti-DIG antibodies (Sigma/Roche, 11093274910). After washes and prior to development ...
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bioRxiv - Developmental Biology 2020Quote: ... washed three times 15 min each in PBST and pre-incubated for 1h at 60°C in Hybridization Buffer (HB: 50% Formamide, 2X SSC, 1mg/ml Torula RNA, 0.05mg/ml, Heparin, 2% Roche blocking reagent ...
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bioRxiv - Evolutionary Biology 2020Quote: ... tissues were incubated at 4°C in a solution of 0.05% alkaline phosphatase-conjugated DIG antibodies (Roche cat #11093274910) overnight ...
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bioRxiv - Immunology 2020Quote: Single-cell suspensions of the lung were prepared by digesting minced lung lobes for 30 min at 37°C with 0.2 U/ml Liberase TL (Roche) and 80 U/ml DNase (Life Tech ...
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bioRxiv - Molecular Biology 2021Quote: ... on a rotating wheel at 4 °C and all buffers were supplemented with protease inhibitor cocktail (#11873580 001, Roche). Prior to elution ...
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bioRxiv - Immunology 2019Quote: Tissues from donor 390c were manually diced and <5.0g was added per Miltenyi C tube with 5mL tissue dissociation media (1x Liberase TL (0.13U/mL/DNase; Roche) and Benzonase nuclease (10U/mL ...
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bioRxiv - Immunology 2020Quote: ... The organs were chopped mechanically with scalpels and transferred into gentleMACS C tubes containing DMEM supplemented with 1μg/mL Collagenase D (Roche) and 25mg/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... tissue was cut into pieces and digested for 15 minutes in a shaking water bath held at 37°C in a 0.5mg/ml solution of Dispase II (Roche) in PBS ...
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bioRxiv - Genomics 2020Quote: ... The quality and quantity of the capture Hi-C libraries was tested by Bioanalyzer and KAPA qPCR (Kapa Biosystems). Capture Hi-C libraries were analysed by 75 bp paired-end Next Generation Sequencing on an Illumina NextSeq500 (My-La ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 μl of SEC samples were either pre-treated for 40 min at 37 °C with DNase I (Roche) at a concentration of 1 IU/μl ...
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bioRxiv - Neuroscience 2021Quote: ... Tween-20 0.3%) for 1 hour and finally incubated overnight at 4°C with anti-Dig-POD antibody (Roche) diluted 1:500 in blocking solution ...
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bioRxiv - Neuroscience 2022Quote: ... Myelin was washed by dilution in HBSS and centrifuged at 400 x g for 5 min at 4 °C before suspending in 3 mL ice cold 0.32 M sucrose solution containing protease inhibitor cocktail (Roche). Next ...
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bioRxiv - Biophysics 2022Quote: ... constructs were resuspended in Buffer C (20 mM at Tris pH 7.6, 300 mM NaCl, 5 mM DTT, and Roche Complete Mini protease inhibitor cocktail (Roche Diagnostics 11836153001)) ...
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bioRxiv - Neuroscience 2022Quote: ... Colonies were picked and grown individually on a feeder layer of MEFs treated with mitomycin C (Roche, Indianapolis, IN) as described previously76 ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes were incubated overnight at 4° C on a rotator with primary antibody in Western Blocking Reagent (Roche), if not stated otherwise ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were incubated overnight at 4°C with monoclonal mouse anti-GFP antibody (Roche; Basel, CH; dilution 1:500) or monoclonal rat anti-HA antibody (Roche ...
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bioRxiv - Physiology 2023Quote: ... enzymatic digestion was carried out for 20-25 min at 36 °C in the low-Ca2+ solution containing Liberase TH Research Grade (0.15 mg/mL; Roche) and elastase (0.5 mg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... the supernatant was incubated at 4°C overnight with 20 μl anti-HA antibody coated beads (Roche, Rotkreuz, Switzerland) at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... the isolated skin was incubated overnight at 4 °C in a tube containing 10 mL of 1x Dispase (Roche) and 2x Pen/Strep (Sigma ...
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bioRxiv - Immunology 2022Quote: ... was added and cells were centrifuged at 350g for 7 min at 4°C and resuspended in PBS with 0.5% BSA (Roche) and 1% FBS (Sorting Buffer) ...
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bioRxiv - Biophysics 2022Quote: ... The transcription mixture was incubated at 37 °C for 4 h and treated with 50U/mL DNAse I (Roche) at 37 °C for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated overnight at 4°C with anti-digoxigenin-alkaline phosphatase antibodies (1:5000; Roche; 11093274910; AB_514497) diluted in blocking solution ...
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bioRxiv - Developmental Biology 2023Quote: ... dissected tissues were dissociated for 30 mins at 37 °C in 200ul of LiberaseTM (Roche, 100ug/ml in PBS). 2 mL of cold PBS (without ca/Mg ...
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bioRxiv - Molecular Biology 2023Quote: ... Mouse atria were minced and digested with mild agitation for 30 minutes at 37°C in DMEM containing 1% P/S and 0.14 Wunsch U ml-1 Liberase (5401119001, Roche). MAFs were enriched via negative selection with magnetic beads against mouse CD45 (leukocytes) ...
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bioRxiv - Genetics 2023Quote: ... incubated the islets for 10 min at 37°C in 2 mL PBS with 4U Dispase I (Roche Diagnostics) / 2U DNase I (ThermoFisher Scientific) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Tissues were washed in three changes of 0.2× SSC at 44°C for 15 minutes each and blocked in 4×SSC containing 1% blocking reagent (Roche) in 4× SSC for 1 hour at RT ...
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bioRxiv - Immunology 2023Quote: ... The DNA samples were treated with 0.5 μg/μl of RNase A for 30 min at 37°C and then with 0.25 μg/μl of Proteinase K (Roche) in the presence of 10mM EDTA ...
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bioRxiv - Cell Biology 2023Quote: ... cardiomyocytes were dissociated using DPBS for 20 min at 37 °C followed by 1:200 Liberase TH (Roche, 5401151001) diluted in DPBS for 20 min at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Primary and secondary antibodies were incubated for 1 h at 37°C while nuclei were counterstained with DAPI (Roche) at RT for 5 min before mounting the glass coverslips using DAKO mounting medium (Agilent) ...
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bioRxiv - Immunology 2023Quote: ... The epidermis and dermis layers were separated after incubation at 37°C for 1h in 0.4 mg/mL dispase II (Roche). The epidermis was collected and then cut into small pieces using scalpels and incubated for 30 minutes with agitation at 37°C in RPMI containing 10% FCS and 0.5 mg/mL DNAse I ...
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bioRxiv - Immunology 2023Quote: ... The epidermis and dermis layers were separated after incubation at 4°C for 16h in 0.2 mg/mL dispase II (Roche). Epidermal layers were fixed in 4% Para-formaldehyde for 20 min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 75 μl of the sample were treated for 30 min at 50°C with 1.2 mg/ml Proteinase K (Roche) in Proteinase K Buffer (100 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... Brain and spinal cord were cut into pieces and digested for 30 min at 37 °C with collagenase D (3.75 mg/ml; Roche) and DNase I (1 mg/ml ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies were incubated overnight at 4°C: rabbit anti-HA high affinity at 1:500 (Roche Diagnostics #ROAHAHA), mouse anti-FLAG M2 at 1:500 (Sigma-Aldrich #F1804) ...
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bioRxiv - Molecular Biology 2023Quote: ... Pre-hybridization was done at 68 °C for 1 h and using DIG Northern Blot Starter KIT (12039672910, Roche). DIG-labeled in vitro transcribed lncMtDloop ...
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bioRxiv - Genetics 2023Quote: ... the mixture was incubated at 42 °C overnight and then added to 10 ml DIG-Easy hybridization solution (Roche).