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Citations for Roche :
7851 - 7900 of 8559 citations for 6 Methyl 2 3 4 9 tetrahydro 1H carbazol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Blotting was done as described in (Konrad et al. 2010) using primary rat-derived anti-HA antibody (dilution 1:500, Roche) followed by anti-rat (dilution 1:2000 ...
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bioRxiv - Immunology 2022Quote: ... Yield of RNA yield was quantitated by nano-drop and 1 µg of RNA was use to reverse-transcribed to cDNA using the iScript cDNA synthesis kit (BIORAD; #1708891) (Roche). 1:5 diluted cDNAs was used for qPCR by using KAPA SYBR® FAST qPCR Master Mix (5X ...
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bioRxiv - Cell Biology 2022Quote: ... then precipitated and cleaned before resuspension in hybridization buffer (65% formamide, 5X SSC, 5X Denhardts with 1% blocking reagent (Roche), 0.5 mg/mL salmon sperm DNA added fresh) ...
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bioRxiv - Microbiology 2020Quote: ... proteins were collected by lysing in lysis buffer (50mM TRIS-HCl, 150mM NaCl, 1% NP40 and protease inhibitor cocktail [Complete Mini EDTA-free, Roche]) and subjected to western blotting with the corresponding antibody (anti-GFP ...
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bioRxiv - Neuroscience 2020Quote: ... Protein aggregation was evaluated by western blot in total cell extracts prepared in 1% Triton X-100 in PBS containing proteases and phosphatases inhibitors (Roche). Protein quantification was performed with the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Neuroscience 2020Quote: Surface expression of HA-C99 and cc-del(X) fusion proteins was measured with monoclonal HA-antibody (Roche 1:100) by flow cytometry as described previously (Staerk et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated for 30 min at 37 °C in a solution (1 ml per well) containing 10 U/ml of dispase II (Roche) and 125 U/ml of collagenase type IV (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated with α-DIG or α-fluorescein antibody conjugated with alkaline phosphatase (Roche, 1:2000 in 10% FBS/PBST) at 4°C overnight ...
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bioRxiv - Plant Biology 2021Quote: ... For IPs 0.3 g of flower buds were ground in 1.5 ml of ice-cold lysis buffer (50 mM Tris-HCl pH 8, 50 mM NaCl, 1 % Triton X-100, supplemented with Roche cOmplete ...
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bioRxiv - Molecular Biology 2021Quote: ... The washed pellet was resuspended in approximately 1/3 of packed cell volume of ORC lysis buffer containing cOmplete Protease Inhibitor Cocktail Tablet (1 tablet per 25 mL total volume; Roche) and frozen dropwise in liquid nitrogen.
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bioRxiv - Microbiology 2020Quote: ... pelleted and resuspended in 150 μl sonication buffer [(50 mM Tris pH 8, 1% SDS, 10 mM EDTA, 1x protease inhibitor (Roche)] ...
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bioRxiv - Molecular Biology 2021Quote: ... un-probed input RNA was also subjected to Sanger sequencing: RNA was dissolved in 5 μl water and supplemented with 1 μl of 10 mM corresponding ddNTP (Roche), then reverse-transcribed into cDNA and analysed by PAGE as described below.
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bioRxiv - Microbiology 2020Quote: ... 30 mM imidazole, 1mM DTT, 0.75% CHAPS, 1 μM DNases, 5◻mM MgCl2 and EDTA-free protease inhibitor cocktail [Roche]). Purification was similar as the above instead of the elution buffer used (50 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: Cells were washed once with ice-cold PBS and lysates harvested at the indicated times post infection with lysis buffer (1% NP-40, 2mM EDTA, 10% glycerol, 150mM NaCl, 50mM Tris HCl) supplemented with protease inhibitors (Roche – complete mini EDTA-free protease inhibitor ...
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bioRxiv - Molecular Biology 2020Quote: ... Explants were diced and then digested by sequential incubations with pronase (70 U/mL for 1 hour at 37°C; Roche) and collagenase (0.2% w/v ...
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bioRxiv - Neuroscience 2020Quote: ... 10 mM Tris-HCl (pH 7.4) and 1 mg/ml BSA) supplemented with 10 μl of DIG RNA labeling mix (Roche 12039672910) and incubated for 1 h at 37°C with occasional gentle tapping ...
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bioRxiv - Neuroscience 2020Quote: ... slides were incubated overnight at room temperature with anti-DIG antibody conjugated with the alkaline phosphatase (1/5,000, Roche Diagnostics) in B1 containing 1% NGS ...
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bioRxiv - Cell Biology 2020Quote: ... protein extracts were prepared as previously described (Alao et al., 2014) with addition of 1 × PhosStop phosphatase inhibitor cocktail (Roche Diagnostics Scandinavia AB ...
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bioRxiv - Cell Biology 2020Quote: ... TNbuffer 10 min at RT and TNT buffer 15’ at RT) and by the incubation of cryo-section using anti-digoxigenin-ap fab fragments (1/100, #11093274910, Roche) dissolved in TNbuffer for 2 hours at RT ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM EDTA) supplemented with 150 mM NaCl and the Completeä Mini EDTA-free protease inhibitor cocktail (Roche Applied Bioscience). Cell aliquots (200 ml ...
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bioRxiv - Immunology 2020Quote: ... 1 mM of EDTA) supplemented with protease and phosphatase inhibitors (EDTA-free protease mixture inhibitor; Roche Diagnostics, Almere, the Netherlands). The protein pellets were homogenized passing through a 27G needle ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBST with 5% milk protein and probed with the following antibodies: 1:1000 anti-HA (Roche), then 1:1500 goat anti-rat IgG-HRP (Dako) ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then pretreated with TdT buffer (25 mm Tris-HCl, 200 mm sodium cacodylate, 0.25 mg/ml BSA, 1 mm cobalt chloride, Roche Diagnostics) at RT for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... RNAseq libraries were prepared from 1 μg of total RNA per sample using the KAPA Stranded mRNA Sequencing Kit (Roche). In short ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were treated for 1 hr at 55⁰C with proteinase K solution (0.4 mg/mL proteinase K (Roche; 3115828001), 50 mM EDTA ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was quantitated by NanoDrop and 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche). Diluted cDNAs (1:5 ...
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bioRxiv - Neuroscience 2022Quote: ... Hippocampi were isolated and stored in 10 volumes of sucrose buffer 1 (320mM sucrose, 1mM NaHCO3, 1mM MgCl2, 0.5mM CaCl2, 1µM PMSF) containing protease inhibitor cocktail (Roche, Mannheim, Germany). Tissues were homogenized for 10 strokes using a motor-driven 2 ml Potter-Elvehjem homogenizer fitted with a Teflon pestle ...
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bioRxiv - Molecular Biology 2022Quote: ... IMAC lysis buffer (1.5 ml buffer per gram cell pellet) and the complete stock solution (1 tablet Complete EDTA-free (protease inhibitor cocktail, Roche) and 50 μl benzonase nuclease cell resuspension (PSF ...
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bioRxiv - Genetics 2022Quote: ... Samples were blocked for 30 minutes in a solution of 1% BSA in 2x SSC with 0.1% tween at room temperature and then rhodamine-conjugated anti-digoxigenin (Roche, #11207750910) or FITC-conjugated anti-biotin (AbCam ...
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bioRxiv - Cell Biology 2022Quote: ... 25mM Tris-HCl pH=7.5, 1% Triton-X, 10mM Na3VO4, 40mM β-glycerophosphate, 10mM NaF and protease cocktail inhibitor from Roche). 1mg of total protein lysate was incubated with 1µg/mL of purified GST or GST-SidI overnight at 4°C in a rotary mixer ...
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bioRxiv - Genetics 2022Quote: ... APRE-19 were cultured in a 100 mm dish for 24 hours and then lysed with 1×NP40 lysis buffer supplemented with protease inhibitors Coaktails (Roche) and phosphatase inhibitors(Roche) ...
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bioRxiv - Genomics 2022Quote: ... Pellets were harvested after 16-18h and resuspended in lysis buffer (50 mM Tris-HCl, 500 mM NaCl, 5% glycerol, 1 tablets per 50 ml protease inhibitor (EDTA-free, Roche), 0.2 mM PMSF ...
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bioRxiv - Cell Biology 2022Quote: ... the RNA concentration was determined and 1 µg RNA was used for the cDNA synthesis according to the Transcriptor First Strand cDNA Synthesis kit (Roche). The expression of the following genes FTSH7 (AT3G47060) ...
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bioRxiv - Cell Biology 2022Quote: 2 μL of FLAG or IgG pull down and of a 1:10 dilution of the INPUT were used for amplification using qPCRBIO SyGreen Mix (PCR Biosystem) in a LightCycler96 (Roche) instrument ...
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bioRxiv - Cancer Biology 2019Quote: Reverse transcription (RT) reactions were carried out using 1 μg of RNA using Transcriptor RT enzyme (Catalog No. 03531287001) from Roche Diagnostics ...
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bioRxiv - Immunology 2019Quote: ... LN were pierced and torn with sharp forceps in 24-well plates and incubated for 15 min at 37° C in 1 ml digestion buffer (100 U/ml collagenase A (Roche), 500 U/ml collagenase D (Roche) ...
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bioRxiv - Neuroscience 2019Quote: ... Remaining pellets were washed with PBS and resuspended in 1% sarkosyl in PBS containing complete mini protease inhibitor cocktail (Roche), vortexed for 30 sec and incubated at room temperature for 1 hr ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were pelleted by centrifugation and resuspended in sonication buffer (50 mM Tris-HCl pH 8, 1% SDS, 10 mM EDTA, 1x cOmplete ULTRA Tablet (Roche)) and sonicated using a Bioruptor sonicator (Diagenode ...
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bioRxiv - Physiology 2019Quote: ... sections were incubated for 1 hrs at 37°C in the dark with the in situ Cell Death Detection Kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Damage to the human colon epithelial cell line HT-29 and macrophages J774A.1 (ATCC TIB-67) were assessed using a lactate dehydrogenase (LDH) cytotoxicity detection kitPLUS (Roche), which measures the release of LDH in the growth medium ...
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bioRxiv - Microbiology 2019Quote: ... resuspended in 1 mL ice-cold lysis buffer (100 mM NaCl, 5% glycerol, 1 mM dithiothreitol and protease inhibitor cocktail (cOmplete, EDTA-free; Roche) in 50 mM Tris HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... the USER digestion product was directly performed index PCR with 1 x KAPA HiFi HotStart Uracil+ ReadyMix (Kapa Biosystems, Cat.No.KK2801). Protocol of Strand Test 3 was almost same as Strand Test 2 ...
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bioRxiv - Neuroscience 2019Quote: ... cells were lysed in Hepes buffer (20 mM Hepes, 1% CHAPS, 100 mM NaCl, 5 mM EDTA, protease inhibitor cocktail (Roche), phosphatase inhibitor cocktail III (Sigma)) ...
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bioRxiv - Biochemistry 2019Quote: ... 10 mM imidazole and 10% glycerol supplemented with EDTA-free protease inhibitor tablets (Roche; 1 tablet per 100 ml buffer) and lysozyme (1 mg/ml) ...
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bioRxiv - Systems Biology 2019Quote: ... 1 mM EDTA) supplemented with phosphatase inhibitors (5 mM b-glycerophosphate, 5 mM NaF, 1 mM Na3VO4 and protease inhibitors (Roche cOmplete ULTRA Tablets ...
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bioRxiv - Genomics 2019Quote: ... amplicons were diluted 1:100 and 1 µL was used into a 40-µL barcoding reaction using 20 µL 2x KAPA HiFi Hotstart reagents (Roche) and 80pmol each barcoded primer ...
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bioRxiv - Cancer Biology 2019Quote: Pancreatic tumors were resected from the mice and mechanically and enzymatically digested in Hanks Balanced Salt Solution (HBSS) with 1 mg/mL Collagenase P (Roche) Cell suspensions were centrifuged for 5 min at 1500 rpm ...
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bioRxiv - Microbiology 2019Quote: Whole cell lysates were prepared by suspending purified parasite pellets in whole cell lysis buffer (1XPBS, 1% v/v Triton X-100) containing Protease Inhibitors (cOmplete EDTA-free, Roche). 24 h in vitro ookinetes were also subjected to cellular fractionation using the following method ...
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bioRxiv - Biochemistry 2019Quote: ... were lysed with lysis buffer (25mM Tris, 150mM NaCl, 1mM EDTA, 1% NP-40, 5% Glyceral) with proteinase inhibitor cocktail (Roche) on ice for 30 min ...
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bioRxiv - Cell Biology 2019Quote: Cell pellets were washed twice with ice-cold PBS prior to lysis in lysis buffer supplemented with 1% Triton X-100 and protease inhibitors (Roche) on ice ...